Affiliations 

  • 1 Centre for Biospectroscopy, School of Chemistry, Faculty of Science, Monash University, Clayton, Victoria 3800, Australia; Department of Food Industry, Faculty of Bioresource and Food Industry, Universiti Sultan Zainal Abidin, 22200 Terengganu, Malaysia; CSIRO Agriculture and Food, Werribee, Victoria 3030, Australia
  • 2 CSIRO Agriculture and Food, Werribee, Victoria 3030, Australia
  • 3 Centre for Biospectroscopy, School of Chemistry, Faculty of Science, Monash University, Clayton, Victoria 3800, Australia. Electronic address: Donald.McNaughton@monash.edu
Food Chem, 2017 Oct 15;233:197-203.
PMID: 28530566 DOI: 10.1016/j.foodchem.2017.04.086

Abstract

Confocal Raman microscopy (CRM) was able to quantify the β-carotene concentration in oil droplets and determine the partitioning characteristics of β-carotene within the emulsion system in situ. The results were validated by a conventional method involving solvent extraction of β-carotene separately from the total emulsion as well as the aqueous phase separated by centrifugation, and quantification by absorption spectrophotometry. CRM also enabled the localization of β-carotene in an emulsion. From the Raman image, the β-carotene partitioning between the aqueous and oil phases of palm olein-in-water emulsions stabilized by whey protein isolate (WPI) was observed. Increasing the concentration of β-carotene in an emulsion (from 0.1 to 0.3g/kg emulsion) with a fixed gross composition (10% palm olein:2% WPI) decreased the concentration of β-carotene in the oil droplet. CRM is a powerful tool for in situ analyses of components in heterogeneous systems such as emulsions.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.