A new thio-Schiff base fluorophore with copper ion sensing, DNA binding and nuclease activity

Vikneswaran R 1 , Syafiq MS 2 , Eltayeb NE 3 , Kamaruddin MN 2 , Ramesh S 4 , Yahya R 5

Affiliations 

  • 1 Centre for Ionics University of Malaya, Department of Physics, Faculty of Science, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia. Electronic address: r.vikneswaran@yahoo.com.my
  • 2 Centre for Ionics University of Malaya, Department of Physics, Faculty of Science, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia
  • 3 Department of Chemistry, Sciences & Arts College - Rabigh, King Abdulaziz University, Rabigh, Saudi Arabia
  • 4 Centre for Ionics University of Malaya, Department of Physics, Faculty of Science, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia. Electronic address: rameshtsubra@gmail.com
  • 5 Department of Chemistry, Faculty of Science, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia. Electronic address: rosiyah@um.edu.my
Spectrochim Acta A Mol Biomol Spectrosc, 2015 Nov 5;150:175-80.
PMID: 26046495 DOI: 10.1016/j.saa.2015.05.087

Abstract

Copper ion recognition and DNA interaction of a newly synthesized fluorescent Schiff base (HPyETSC) were investigated using UV-vis and fluorescent spectroscopy. Examination using these two techniques revealed that the detection of copper by HPyETSC is highly sensitive and selective, with a detection limit of 0.39 μm and the mode of interaction between HPyETSC and DNA is electrostatic, with a binding constant of 8.97×10(4) M(-1). Furthermore, gel electrophoresis studies showed that HPyETSC exhibited nuclease activity through oxidative pathway.

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