Antioxidant potential assessment of phenolic and flavonoid rich fractions of Clematis orientalis and Clematis ispahanica (Ranunculaceae)

Karimi E 1 , Ghorbani Nohooji M 2 , Habibi M 3 , Ebrahimi M 4 , Mehrafarin A 2 , Khalighi-Sigaroodi F 2

Affiliations 

  • 1 a Faculty of Science, Department of Biochemistry and Biophysics , Islamic Azad University, Mashhad Branch , Mashhad , Iran
  • 2 b Medicinal Plants Research Center , Institute of Medicinal Plants, ACECR , Karaj , Iran
  • 3 c Faculty of Science and Engineering, Department of Biology , Gonbad Kavous University , Gonbad Kavous , Iran
  • 4 d Faculty of Veterinary Medicine, Department of Veterinary Preclinical Sciences , Universiti Putra Malaysia , Serdang , Malaysia
Nat Prod Res, 2018 Aug;32(16):1991-1995.
PMID: 28774179 DOI: 10.1080/14786419.2017.1359171

Abstract

The antioxidant activities of crude extract fractions using Hexane, Chloroform, Ethyl Acetate, Butanol and Water of Clematis orientalis and Clematis ispahanica were investigated. 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay and the ferric reducing/antioxidant potential (FRAP) were used to evaluate the antioxidant capacity. The total phenolics were found to be 4.37-9.38 and 1.32-11.37 mg gallic acid equivalents (GAE)/g in different fractions for C. orientalis and C. ispahanica, respectively. The ethyl acetate fraction of C. orientalis and chloroform fraction of C. ispahanica showed the highest DPPH and FRAP activities at a concentration of 300 μg/mL. The predominant phenolic compounds identified by HPLC in C. orientalis were Resorcinol (603.5 μg/g DW) in chloroform fraction and Ellagic acid (811.7 μg/g DW) in chloroform fraction of C. ispahanica.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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