LC-MS/MS quantitation of antimalarial drug piperaquine and metabolites in human plasma

Aziz MY 1 , Hoffmann KJ 2 , Ashton M 3

Affiliations 

  • 1 Unit for Pharmacokinetics and Drug Metabolism, Dept. Pharmacology, Sahlgrenska Academy at University of Gothenburg, Sweden; Advanced Medical and Dental Institute, Universiti Sains Malaysia (USM), Malaysia
  • 2 Unit for Pharmacokinetics and Drug Metabolism, Dept. Pharmacology, Sahlgrenska Academy at University of Gothenburg, Sweden
  • 3 Unit for Pharmacokinetics and Drug Metabolism, Dept. Pharmacology, Sahlgrenska Academy at University of Gothenburg, Sweden. Electronic address: michael.ashton@gu.se
J Chromatogr B Analyt Technol Biomed Life Sci, 2017 Sep 15;1063:253-258.
PMID: 28863865 DOI: 10.1016/j.jchromb.2017.06.035

Abstract

PURPOSE: This study aimed to develop a sensitive, quantitative assay for the antimalarial piperaquine (PQ) and its metabolites M1 and M2 in human plasma.

RESULTS: Analytes were gradiently separated on a C18 column and detected with a Sciex API 4000 MS/MS with an ESI source operated in the positive ion mode with deuterated PQ as internal standard. The response was linear in the range 3.9-2508nM with a runtime of 7.0min per sample. The method was applied to clinical samples from healthy volunteers.

CONCLUSION: This LC-MS/MS method for the simultaneous quantitation of PQ and two of its metabolites in plasma may prove helpful for assessment of metabolite safety issues in vivo.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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