• 1 Laboratory of Animal Behavior and Environmental Science, School of Agriculture, Meiji University, Kanagawa, 214-8571, Japan
  • 2 Center for Material Cycles and Waste Management Research, National Institute for Environmental Studies, Tsukuba, 305-8506, Japan
  • 3 Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Tokyo, 183-0054, Japan
  • 4 Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University, Okayama, 701-4303, Japan
  • 5 Brain Research Institute, School of Medicine and Health Sciences, Monash University, PJ, 46150, Malaysia
  • 6 Laboratory of Animal Physiology, Department of Animal Sciences, Teikyo University of Science, Yamanashi, 409-0193, Japan
J Appl Toxicol, 2018 05;38(5):649-655.
PMID: 29271492 DOI: 10.1002/jat.3569


Exposure to endocrine-disrupting chemicals may adversely affect animals, particularly during development. Tris(1,3-dichloroisopropyl) phosphate (TDCIPP) is an organophosphate with anti-androgen function in vitro that is present in indoor dust at relatively high concentrations. In male rats, androgens are necessary for the development of reproductive organs, as well as the endocrine and central nervous systems. However, we currently do not know the exact effects of TDCIPP exposure through suckling on subsequent reproductive behavior in males. Here, we show that TDCIPP exposure (25-250 mg kg-1 via oral administration over 28 consecutive days post-birth) suppressed male sexual behavior and reduced testes size. These changes were dose-dependent and appeared first in adults rather than in juveniles. These results demonstrate that TDCIPP exposure led to normal body growth and appearance in juveniles, but disrupted the endocrine system and physiology in adults. Therefore, assays should be performed using adult animals to ensure accuracy, and to confirm the influence of chemical substances given during early mammalian life.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.