Affiliations 

  • 1 Nanotechnology and Catalysis Research Centre (NANOCAT), Institute of Graduate Studies, University of Malaya, Kuala Lumpur 50603, Malaysia
  • 2 Nanotechnology and Catalysis Research Centre (NANOCAT), Institute of Graduate Studies, University of Malaya, Kuala Lumpur 50603, Malaysia; Centre for Research in Biotechnology for Agriculture (CEBAR), University of Malaya, Kuala Lumpur 50603, Malaysia; Institute of Halal Research University Malaya (IHRUM), University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: eaqubali@um.edu.my
  • 3 Deparment of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University, Kuantan 25200, Pahang, Malaysia
Food Res Int, 2018 03;105:19-28.
PMID: 29433207 DOI: 10.1016/j.foodres.2017.10.065

Abstract

Species substitution, the use of a low value fish in place of a high value fish, is the biggest problem in international trade and the leading cause of fraud in the fisheries arena sector. Current DNA barcoding systems have partly solved this problem but also failed in many instances to amplify PCR targets from highly processed products because of the degradation of a longer barcode marker (~650bp). In the present study, a novel mini barcode marker (295bp) was developed to discriminate fish species in raw and processed states forms. The barcode primers were cross-tested against 33 fish species and 15 other animal species and found to be universal for all the tested fish varieties. When 20 commercial fish products of five different categories were screened, all commercial fish sample yielded positive bands for the novel fish barcode. PCR product was sequenced to retrieve the species IDs that reflected 55% (11/20) of Malaysian fish products were mislabeled.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.