This study aimed to determine the best parameters (types of buffer, hydrolysis time and enzyme concentration) used to produce good quality of liquid protein hydrolysate from Yellowstripe scad in terms of high yield, protein content and concentration. The choice of buffer (sodium or potassium buffer), hydrolysis time (1 h, 2 h, 3 h or 4 h) and enzymes concentrations (0.5%, 1.0%, 1.5% and 2.0%) were investigated. The results obtained from two way ANOVA showed that these parameters had significant difference (p