Identification and Quantification of Quercetin, A Major Constituent of Artocarpus altilis by Targeting Related Genes of Apoptosis and Cell Cycle: In Vitro Cytotoxic Activity Against Human Lung Carcinoma Cell Lines

Jalal TK 1 , Khan AYF 1 , Natto HA 1 , Abdull Rasad MSB 1 , Arifin Kaderi M 1 , Mohammad M 1 Show all authors , Johan MF 2 , Omar MN 3 , Abdul Wahab R 1

Affiliations 

  • 1 a Department of Biomedical Sciences , Kulliyyah of Allied Health Sciences, International Islamic University Malaysia (IIUM), Jalan Sultan Ahmad Shah, Bandar Indera Mahkota , Kuantan , Pahang , Malaysia
  • 2 c Department of Haematology, School of Medical Sciences , Universiti Sains Malaysia , Kubang Kerian , Kelantan , Malaysia
  • 3 d Department of Biotechnology Sciences , Kulliyyah of Science, International Islamic University Malaysia (IIUM), Jalan Sultan Ahmad Shah, Bandar Indera Mahkota , Kuantan , Pahang , Malaysia
Nutr Cancer, 2019;71(5):792-805.
PMID: 30614285 DOI: 10.1080/01635581.2018.1516790

Abstract

Nine phenolic compounds were identified and quantified in Artocarpus altilia fruit. One of the main compounds was quercetin, which is the major class of flavonoids has been identified and quantified in pulp part of A. altilis fruit of methanol extract. The aim of this study was to evaluate in vitro cytotoxic assay. Inhibitory concentration 50% concentration was determined using trypan blue exclusion assay. Apoptosis induction and cell cycle regulation were studied by flow cytometric analysis. The expression of apoptosis and cell cycle-related regulatory genes were assessed by RT-qPCR study of the methanol extract of pulp part on human lung carcinoma (A549) cell line. A significant increase of cells at G2/M phases was detected (P 

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