Fulltext

Expression profile and down-regulation of argininosuccinate synthetase in hepatocellular carcinoma in a transgenic mouse model

Shiue SC 1 , Huang MZ 2 , Tsai TF 3 , Chang AC 4 , Choo KB 5 , Huang CJ 6 Show all authors , Su TS 7

Affiliations 

  • 1 Institute of Microbiology & Immunology, National Yang-Ming University, Taipei, Taiwan. benson.shiue@gmail.com
  • 2 Department of Medical Research, Taipei Veterans General Hospital, Taipei, 112, Taiwan. mzhuang@vghtpe.gov.tw
  • 3 Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Taipei, Taiwan. tftsai@ym.edu.tw
  • 4 Institute of Neuroscience, National Yang-Ming University, Taipei, Taiwan. acchang@ym.edu.tw
  • 5 Department of Preclinical Sciences, Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, Selangor, Malaysia. chookb@utar.edu.my
  • 6 Department of Animal Science, Chinese Culture University, Taipei, Taiwan. hqr2@faculty.pccu.edu.tw
  • 7 Institute of Microbiology & Immunology, National Yang-Ming University, Taipei, Taiwan. su31659@gmail.com
J Biomed Sci, 2015;22:10.
PMID: 25616743 DOI: 10.1186/s12929-015-0114-6

Abstract

Argininosuccinate synthetase (ASS) participates in urea and nitric oxide production and is a rate-limiting enzyme in arginine biosynthesis. Regulation of ASS expression appears complex and dynamic. In addition to transcriptional regulation, a novel post-transcriptional regulation affecting nuclear precursor RNA stability has been reported. Moreover, many cancers, including hepatocellular carcinoma (HCC), have been found not to express ASS mRNA; therefore, they are auxotrophic for arginine. To study when and where ASS is expressed and whether post-transcriptional regulation is undermined in particular temporal and spatial expression and in pathological events such as HCC, we set up a transgenic mouse system with modified BAC (bacterial artificial chromosome) carrying the human ASS gene tagged with an EGFP reporter.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

MeSH terms
Similar publications