Chlorogenic acid attenuates kidney fibrosis via antifibrotic action of BMP-7 and HGF

Yunus J 1 , Salman M 2 , Lintin GBR 2 , Muchtar M 3 , Sari DCR 4 , Arfian N 5 Show all authors , Romi MM 5

Affiliations 

  • 1 Universitas Gadjah Mada, Public Health, and Nursing, Faculty of Medicine, Department of Anatomy, Jl. Farmako Sekip Utara, Yogyakarta, Indonesia. junaedy_yunus@ugm.ac.id
  • 2 Universitas Tadulako, Faculty of Medicine, Department of Anatomy, Tondo, Mantikulore, Palu City, Central Sulawesi, Indonesia
  • 3 Universitas Alkhairaat, Faculty of Medicine, Department of Anatomy, Jl. Diponegoro, Palu City, Central Sulawesi, Indonesia
  • 4 International Medical University, Jalan Rasah, 70300 Seremban, Negeri Sembilan, Malaysia
  • 5 Universitas Gadjah Mada, Public Health, and Nursing, Faculty of Medicine, Department of Anatomy, Jl. Farmako Sekip Utara, Yogyakarta, Indonesia
Med J Malaysia, 2020 05;75(Suppl 1):5-9.
PMID: 32471962

Abstract

BACKGROUND: Kidney fibrosis, characterised by tubulointerstitial fibrosis, is a histological landmark of chronic kidney disease. The body attempts to compensate for progressive detrimental process of kidney fibrosis by producing antifibrotic substances, such as bone morphogenetic protein-7 (BMP-7) and hepatocyte growth factor (HGF). Chlorogenic acid is known to have renoprotective and antifibrotic properties. This study aims to evaluate the effect of chlorogenic acid on unilateral ureteral obstruction (UUO)-induced kidney fibrosis mice model.

METHODS: This study was a quasi-experimental with posttestonly control group design. Twenty-five adult male Swiss Webster mice were randomly divided into five groups: shamoperated group (SO), UUO-control day-7 (U7), UUO-control day-14 (U14), UUO-chlorogenic acid day-7 (UC7), and UUOchlorogenic acid day 14 (UC14). Myofibroblasts were identified by immunohistochemical staining of alphasmooth muscle actin (α-SMA) while collagen fibers were identified by Sirius Red staining. Both data were presented as area fraction. BMP-7 and HGF mRNA expressions were assessed by reverse transcription PCR (RT-PCR). Data were quantified using ImageJ software.

RESULTS: UUO-control groups (U7 and U14) showed higher α- SMA-immunopositive (6.52±1.33, 18.24±1.39 vs. 0.22±0.01; p<0.05) and Sirius Red-positive area fractions (6.61±0.8, 12.98±2.31 vs. 0.62±0.10; p<0.05), lower BMP-7 (1.02±0.47, 1.18±0.65 vs. 2.09±0.87; p<0.05) and HGF mRNA expressions (1.06±0.31, 0.89±0.14 vs. 1.88±0.81; p<0.05) compared to SO group. UUO-chlorogenic acid groups (UC7 and UC14) showed lower α-SMA-immunopositive (1.24±0.37, 4.58±0.61; p<0.05) and Sirius Red-positive area fractions (4.76±1.03, 3.72±0.54; p<0.05), higher BMP-7 (1.84±0.49, 2.19±0.43; p<0.05) and HGF (1.58±0.38; p>0.05, 1.84±0.42; p<0.05) mRNA expressions compared to UUO-control groups. UUOchlorogenic acid groups showed BMP-7 and HGF mRNA expressions that were not significantly different from the SO group.

CONCLUSION: Chlorogenic acid administration prevents kidney fibrosis in UUO mice model through modulating antifibrotic pathway.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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