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MicroRNA-155 complementation on a chemically functionalized dual electrode surface for determining breast cancer

Gopinath SCB 1 , Perumal V 2 , Xuan S 3

Affiliations 

  • 1 School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600 Arau, Perlis Malaysia
  • 2 Mechanical Engineering Department, Universiti Teknologi PETRONAS, 32610 Seri Iskandar, Perak Darul Ridzuan Malaysia
  • 3 Department of Mammary and Thyroid Surgery, Central Hospital Affiliated to Shandong First Medical University, No. 105 Jiefang Road, Lixia District, 250013 Jinan, Shandong Province People's Republic of China
3 Biotech, 2020 Jun;10(6):270.
PMID: 32523864 DOI: 10.1007/s13205-020-02261-x

Abstract

This study correlated and quantified the expression of microRNA-155 with breast cancer to determine breast cancer progression. The target microRNA-155 sequence was identified by complementation on a capture-probe sequence-immobilized interdigitated dual electrode surface. The sensitivity was found to be 1 fM, and the limit of detection fell between 1 and 10 fM. The specific sequence selectivity with single mismatches, triple mismatches, and noncomplementary bases failed to complement the capture-probe sequence. The obtained results demonstrate the selective determination of the microRNA-155 sequence and can help to diagnose breast cancer.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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