Affiliations 

  • 1 Department of Tuberculosis Complications, Xi'an Chest Hospital, East Section of Aerospace Avenue, Chang'an District, Xi'an, 710100 Shaanxi China
  • 2 2School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600 Arau, Malaysia
3 Biotech, 2020 May;10(5):227.
PMID: 32373419 DOI: 10.1007/s13205-020-02216-2

Abstract

Herein, a rapid and sensitive current-volt measurement was developed for identifying the IS6110 DNA sequence to diagnose Mycobacterium tuberculosis (TB). An aminated capture probe was immobilized on a 1,1'-carbonyldiimidazole-functionalized interdigitated electrode (IDE) silica substrate, and the target sequence was detected by complementation. It was found that all tested concentrations displayed a higher response in current changes than the control, and the limit of detection was 10 fM. The sensitivity ranged from 1 to 10 fM. The control sequences with single-, triple-mismatch and noncomplementary sequences showed great discrimination. This rapid and easy DNA detection method helps to identify M. tuberculosis for early-stage diagnosis of TB.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.