Displaying publications 1 - 20 of 124 in total

  1. Gunasekaran, G., Muhamad Fitri C.A., Chandrashegkar, S., Hajar Amalnina A.B., Raishan, S., Nurul Faiznani, Z., et al.
    Medicine & Health, 2017;12(2):202-209.
    Madu gelam telah didapati mempunyai kesan anti-oksidatif, anti-kanser dan antiinflamasi
    terhadap banyak jenis kanser. Tujuan kajian ini adalah untuk menentukan
    kesan madu gelam terhadap aras tekanan oksidatif sel kanser peparu manusia.
    IC50 madu gelam ditentukan dengan merawat sel A549 dengan dos madu yang
    berbeza (50-200 mg/ml). Sel dibahagikan kepada 4 kumpulan dan diaruh tekanan
    oksidatif dengan menggunakan hidrogen peroksida (H2
    ) mengikut kumpulan
    tertentu: kawalan, H2
    , madu gelam, H2
    + madu gelam. Selepas 24 jam
    rawatan, biopetanda tekanan oksidatif seperti malondialdehid (MDA) dan protein
    karbonil ditentukan. Aruhan tekanan oksidatif meningkatkan aras MDA (p
    Matched MeSH terms: Diagnostic Tests, Routine
  2. Maria Abu Bakar, Azman Jalar, Roslina Ismail
    Sains Malaysiana, 2018;47:805-810.
    Pencerapan dua dimensi (2D) yang diperoleh daripada keratan rentas sampel selalunya digunakan untuk mendapatkan
    maklumat ketebalan lapisan sebatian antara logam (IMC). Walau bagaimanapun, pencerapan 2D amatlah terhad
    berbanding maklumat yang sepatutnya diperoleh daripada struktur tiga dimensi (3D) sampel. Kajian ini bertujuan untuk
    menilai semula kaedah pengukuran lapisan IMC daripada pemerhatian 2D dengan mempertimbangkan faktor-faktor
    sebenar dalam bentuk 3D IMC. Nilai mod ketebalan didapati lebih mewakili nilai ketebalan IMC setelah mempertimbangkan
    pelbagai faktor stereometri berbanding dengan nilai purata. Ini memberikan suatu penanda aras dalam aktiviti
    menentukan ketebalan lapisan IMC berdasarkan pencerapan 2D daripada struktur 3D.
    Matched MeSH terms: Diagnostic Tests, Routine
  3. Lee GE
    Data Brief, 2020 Feb;28:104958.
    PMID: 31890799 DOI: 10.1016/j.dib.2019.104958
    The morphological data of Malesian Lejeunea is described in detail based on examination of about 600 fresh specimens and 1500 herbarium specimens of Lejeunea from other herbaria. Scanning electron microscopy (SEM) images and photographs illustrating the characters of Lejeunea are presented.
    Matched MeSH terms: Diagnostic Tests, Routine
  4. Naing C, Htet NH, Aye SN, Aung HH, Tanner M, Whittaker MA
    Malar J, 2022 Feb 16;21(1):50.
    PMID: 35172833 DOI: 10.1186/s12936-022-04082-0
    BACKGROUND: Achieving malaria elimination requires the targeting of the human reservoir of infection, including those patients with asymptomatic infection. The objective was to synthesise evidence on the accuracy of the rapid-onsite diagnostic tests (RDTs) and microscopy for the detection of asymptomatic malaria as part of the surveillance activities in Asian countries.

    METHODS: This was a meta-analysis of diagnostic test accuracy. Relevant studies that evaluated the diagnostic performance of RDTs and microscopy for detection of asymptomatic malaria were searched in health-related electronic databases. The methodological quality of the studies included was assessed using the QUADAS-2 tool.

    RESULTS: Ten studies assessing RDT and/or microscopy were identified. The diagnostic accuracies in all these studies were verified by PCR. Overall, the pooled sensitivities of RDT, as well as microscopy for detection of any malaria parasites in asymptomatic participants, were low, while their pooled specificities were almost ideal. For the detection of Plasmodium falciparum, pooled sensitivity by RDT (59%, 95%CI:16-91%) or microscopy (55%, 95%CI: 25-82%) were almost comparable. For detection of Plasmodium vivax, pooled sensitivity of RDT (51%, 95% CI:7-94%) had also the comparable accuracy of microscopy (54%, 95%CI,11-92%). Of note are the wide range of sensitivity and specificity.

    CONCLUSION: The findings of this meta-analysis suggest that RDTs and microscopy have limited sensitivity and are inappropriate for the detection of asymptomatic Plasmodium infections. Other methods including a combination of PCR-based strategies, Loop-Mediated Isothermal Amplification (LAMP) technique must be considered to target these infections, in order to achieve malaria elimination. However, more data is needed for the wide acceptance and feasibility of these approaches. Studies to explore the role of asymptomatic and sub-patent infections in the transmission of malaria are of critical importance and are recommended.

    Matched MeSH terms: Diagnostic Tests, Routine/methods
  5. Phing SH, Mazhorova A, Shalaby M, Peccianti M, Clerici M, Pasquazi A, et al.
    Sci Rep, 2015;5:8551.
    PMID: 25711343 DOI: 10.1038/srep08551
    Terahertz technologies recently emerged as outstanding candidates for a variety of applications in such sectors as security, biomedical, pharmaceutical, aero spatial, etc. Imaging the terahertz field, however, still remains a challenge, particularly when sub-wavelength resolutions are involved. Here we demonstrate an all-optical technique for the terahertz near-field imaging directly at the source plane. A thin layer (<100 nm-thickness) of photo carriers is induced on the surface of the terahertz generation crystal, which acts as an all-optical, virtual blade for terahertz near-field imaging via a knife-edge technique. Remarkably, and in spite of the fact that the proposed approach does not require any mechanical probe, such as tips or apertures, we are able to demonstrate the imaging of a terahertz source with deeply sub-wavelength features (<30 μm) directly in its emission plane.
    Matched MeSH terms: Diagnostic Tests, Routine
  6. Choo SW, Wong YL, Beh CY, Lokanathan N, Leong ML, Ong CS, et al.
    Genome Announc, 2013 Jan;1(1).
    PMID: 23405341 DOI: 10.1128/genomeA.00063-12
    Mycobacterium abscessus is an emerging clinical pathogen commonly associated with non-tuberculous mycobacterial infections. We report herein the draft genome of M. abscessus strain M156.
    Matched MeSH terms: Diagnostic Tests, Routine
  7. Chua KH, Lee PC, Chai HC
    Malar J, 2016;15:134.
    PMID: 26931146 DOI: 10.1186/s12936-016-1183-z
    Detection of Plasmodium spp. is sometimes inconvenient especially in rural areas that are distant from a laboratory. In this study a portable diagnostic test of Plasmodium spp. was developed using insulated isothermal polymerase chain reaction (iiPCR) as an alternative approach to improve this situation.
    Matched MeSH terms: Diagnostic Tests, Routine
  8. Kareem KY, Hooi Ling F, Teck Chwen L, May Foong O, Anjas Asmara S
    Gut Pathog, 2014;6:23.
    PMID: 24991236 DOI: 10.1186/1757-4749-6-23
    The present study aimed to determine the inhibitory activity of postbiotic produced by L. plantarum using reconstituted media supplemented with different levels of inulin and to select the best combination based on the modified inhibitory activity (MAU/mL) against pathogens.
    Matched MeSH terms: Diagnostic Tests, Routine
  9. Al-Hardan N, Abdullah M, Abdul Aziz A, Ahmad H
    Sains Malaysiana, 2011;40:1123-1127.
    A ZnO gas sensor was successfully prepared by RF sputtering. The maximum sensitivity of the sensor for vinegar test application was at 400oC. The ZnO based sensor showed good sensitivity for vinegar test in the concentration range of 4% to 9%. The work reveals the ability of using ZnO gas sensor to determine the acid concentrations of the vinegars for food requirements.
    Matched MeSH terms: Diagnostic Tests, Routine
  10. Suzana S, Nik Nur Izzati N, Siti Hajar M, Hasnah H, Kamaruddin M
    Sains Malaysiana, 2016;45:1823-1834.
    Intervensi pemakanan berpotensi untuk melambatkan penurunan fungsi kognitif dalam kalangan warga tua yang mengalami kecelaan kognitif ringan (MCI). Walau bagaimanapun, kemampanan program sedemikian masih belum dapat dipastikan. Oleh itu, suatu kajian susulan dijalankan bagi menentukan kemampanan suplementasi minyak ikan dan pendidikan kesihatan bagi mengurangkan kemerosotan fungsi kognitif dalam kalangan warga tua yang mengalami MCI selepas setahun kajian intervensi dijalankan. Kajian ini melibatkan 18 orang subjek MCI iaitu 6 orang bagi setiap kumpulan intervensi suplementasi minyak ikan (A) dan pendidikan kesihatan (B) dan kumpulan kawalan (C). Analisis menunjukkan kesan intervensi yang signifikan bagi ujian Span Digit (p<0.05) dan aras serum folat (p<0.05) dengan kumpulan B menunjukkan min yang paling tinggi, diikuti dengan A dan C. Intervensi pendidikan kesihatan dan suplementasi minyak ikan mempunyai kesan kemampanan bagi meningkatkan aras serum folat dan memperbaiki status fungsi kognitif yang dinilai menggunakan Ujian Span Digit. Terdapat keperluan bagi promosi kesihatan dan suplementasi minyak ikan dalam mengekalkan fungsi kognitif individu warga tua yang mengalami MCI
    Matched MeSH terms: Diagnostic Tests, Routine
  11. Lee CK
    Med J Malaysia, 2007 Mar;62(1):1-2.
    PMID: 17682559 MyJurnal
    In 1985, when HIV testing first became available, the main goal of such testing was to ensure blood safety. Hemophiliacs and other patients who were transfusion-dependent were the initial patients that needed to be protected as they were exposed to iatrogenic risk. It dawned very quickly to health authorities even then that alternative testing sites had to be quickly established to deter persons from using blood bank facilities for HIV testing purposes. At that time, professional opinion was divided regarding the value of HIV testing and whether HIV testing should be encouraged because no consensus existed regarding whether a positive test predicted transmission to sex partners or from mother to infant. No effective treatment existed then and stigma and discrimination faced by those who were found positive was rampant in many parts of the world. Counseling was designed, in part, to ensure that persons tested were aware of the implications of a positive result and in part, to address the person’s risk behavior to reduce transmission to others.
    Matched MeSH terms: Diagnostic Tests, Routine*
  12. Selvarajah D, Naing C, Htet NH, Mak JW
    Malar J, 2020 Jun 19;19(1):211.
    PMID: 32560728 DOI: 10.1186/s12936-020-03283-9
    BACKGROUND: The global malaria decline has stalled and only a few countries are pushing towards pre-elimination. The aim of the malaria elimination phase is interruption of local transmission of a specified malaria parasite in a defined geographical area. New and improved screening tools and strategies are required for detection and management of very low-density parasitaemia in the field. The objective of this study was to synthesize evidence on the diagnostic accuracy of loop-mediated isothermal amplification (LAMP) test for the detection of malaria parasites among people living in endemic areas.

    METHODS: This study adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis for Diagnostic Test Accuracy (PRISMA-DTA) guideline. Relevant studies in the health-related electronic databases were searched. According to the criteria set for this study, eligible studies were identified. The quality of included studies was evaluated with the use of a quality assessment checklist. A summary performance estimates such as pooled sensitivity and specificity were stratified by type of LAMP. Bivariate model for data analyses was applied. Summary receiver operating characteristics plots were created to display the results of individual studies in a receiver operating characteristics space. Meta-regression analysis was performed to investigate the sources of heterogeneity among individual studies.

    RESULTS: Twenty-seven studies across 17 endemic countries were identified. The vast majority of studies were with unclear risk of bias in the selection of index test. Overall, the pooled test performances were high for Pan LAMP (sensitivity: 0.95, 95% CI 0.91 to 0.97; specificity: 0.98, 95% CI 0.95 to 0.99), Plasmodium falciparum (Pf) LAMP (sensitivity: 0.96, 95% CI 0.94 to 0.98; specificity: 0.99, 95% CI 0.96 to 1.00) or for Plasmodium vivax (Pv) LAMP from 6 studies (sensitivity: 0.98, 95% CI 0.92 to 0.99; specificity: 0.99, 95% CI 0.72 to 1.00). The area under the curve for Pan LAMP (0.99, 95% CI 0.98-1.00), Pf LAMP (0.99, 95% CI 0.97-0.99) and Pv LAMP was (1.00, 95% CI 0.98-1.00) indicated that the diagnostic performance of these tests were within the excellent accuracy range. Meta-regression analysis showed that sample size had the greatest impact on test performance, among other factors.

    CONCLUSIONS: The current findings suggest that LAMP-based assays are appropriate for detecting low-level malaria parasite infections in the field and would become valuable tools for malaria control and elimination programmes. Future well-designed larger sample studies on LAMP assessment in passive and active malaria surveillances that use PCR as the reference standard and provide sufficient data to construct 2 × 2 diagnostic table are needed.

    Matched MeSH terms: Diagnostic Tests, Routine/methods*
  13. Abdul Rahim FH, Thambiah CS, Samsudin IN, Mohamed Mokhtar N
    Malays J Pathol, 2020 Aug;42(2):297-300.
    PMID: 32860386
    INTRODUCTION: Cystic fibrosis (CF) is a life-limiting autosomal recessive disorder as a result of CF transmembrane conductance regulator gene mutation. It has a wide range of disease severity in patients with the same genotype.

    CASE REPORT: A 5-year-old Malay boy with a history of recurrent pneumonia, presented with productive cough, fever and worsening tachypnoea. Physical examination revealed coarse crepitations, reduced breath sounds and clubbing. Biochemical investigations showed that he had respiratory type 2 failure as a result of bronchiectasis. Sweat conductivity done twice was raised supporting a diagnosis of CF. Other investigations such as bronchoscopy to look for congenital anomaly of the lung, infectious disease screening and tuberculosis, fungal and viral culture and sensitivity were negative. Further cascade screening revealed high sweat conductivity results in his siblings.

    DISCUSSION: Although CF prevalence is low in Malaysia, it is nevertheless an important diagnosis to be recognised as it is associated with increased morbidity.

    Matched MeSH terms: Diagnostic Tests, Routine/methods
  14. Ismail A, Teh LK, Ngeow YF, Norazmi MN, Zainul ZF, Tang TH, et al.
    Genome Announc, 2013;1(3).
    PMID: 23788553 DOI: 10.1128/genomeA.00397-13
    We report the annotated genome sequence of a clinical isolate, Mycobacterium tuberculosis strain PR05, which was isolated from the human cerebrospinal fluid of a patient diagnosed with tuberculosis.
    Matched MeSH terms: Diagnostic Tests, Routine
  15. Kohno A, Nik Farid ND, Musa G, Abdul Aziz N, Nakayama T, Dahlui M
    BMJ Open, 2016;6(3):e010668.
    PMID: 27006344 DOI: 10.1136/bmjopen-2015-010668
    While living overseas in another culture, retirees need to adapt to a new environment but often this causes difficulties, particularly among those elderly who require healthcare services. This study examines factors affecting healthcare service utilisation among Japanese retirees in Malaysia.
    Matched MeSH terms: Diagnostic Tests, Routine
  16. Issa R, Seradja VH, Abdullah MK, Abdul H
    Genome Announc, 2016;4(3).
    PMID: 27340055 DOI: 10.1128/genomeA.00517-16
    This is a report of the annotated genome sequence of Mycobacterium tuberculosis MTBR3/09. The organism was isolated from a sputum sample in Malaysia.
    Matched MeSH terms: Diagnostic Tests, Routine
  17. Issa R, Seradja VH, Abdullah MK
    Genome Announc, 2016;4(3).
    PMID: 27365342 DOI: 10.1128/genomeA.00376-16
    Here, we report of the annotated genome sequence of Mycobacterium tuberculosis MTB221/11. The organism was isolated from the cerebrospinal fluid of a patient in Malaysia.
    Matched MeSH terms: Diagnostic Tests, Routine
  18. Syarifah Haizan Sayed Kamar, Nasruddin Jaafar
    Mothers can be trained to screen caries in their children. The aim of this study is to
    assess the validity of mothers’ caries screening of their preschool children. (Copied from article).
    Matched MeSH terms: Diagnostic Tests, Routine
  19. Alifah Nadia Abu Hassan, Ezalia Esa, Nur Aisyah Aziz, Faidatul Syazlin Abd Hamid, Zubaidah Zakaria, Siti Aisyah Lazim
    Thalassaemia screening programme was conducted to reduce the burden of the disease [1]. Here, we describe one unexpected discovery in a 33-year-old gentleman and also the importance of DNA analysis in detecting the globin gene mutation.
    Matched MeSH terms: Diagnostic Tests, Routine
  20. Low GK, Looi SY, Yong MH, Sharma D
    J Vector Borne Dis, 2018 10 4;55(2):79-88.
    PMID: 30280705 DOI: 10.4103/0972-9062.242568
    Diagnosing severe dengue from those who do not develop complication is important to prevent death. The objective of this systematic review was to evaluate the diagnostic test accuracy of ultrasonography in differentiating severe dengue from nonsevere dengue; and to assess if ultrasonography/ultrasound can be used as a predictive (screening) and diagnostic tool in the course of dengue infection. An electronic search was conducted in different databases via OvidSP platform. The included studies were cohort studies between 1995 and 2016 wherein cases were confirmed by dengue blood test. Severity of dengue was assessed and compared using standard WHO references. The methodological quality of the paper was assessed by two independent reviewers by using QUADAS-2 tool. In total 12 studies were included in this review after suitable screening. Overall, the studies included had a low and unclear risk of bias. Seven out of nine studies that compared severe dengue and nonsevere dengue, performed an ultrasonography on gallbladder (wall thickness cutoff-3 mm) with a sensitivity of 24.2-100% and a specificity of 13.2-98.7%. Other parameters such as splenic subcapsular fluid collection, pericardial fluid and hepatic subcapsular fluid collection had a specificity of >90%, though the sensitivity was poor. There were insufficient evidence that ultrasonography is able to differentiate severe dengue from nonsevere dengue accurately. The predictive and diagnostic value of ultrasonography could not be concluded due to insufficient reporting on the temporality of the ultrasonography performed with regard to the diagnosis. However, it might serve as an adjunct investigation to support the clinical diagnosis.
    Matched MeSH terms: Diagnostic Tests, Routine
Contact Us

Please provide feedback to Administrator (tengcl@gmail.com)

External Links