Affiliations 

  • 1 Key Laboratory of Straw Biology and Utilization, Ministry of Education, College of Life Science, JiLin Agricultural University, Changchun, 130000, JiLin, China; Innovation Platform of Straw Comprehensive Utilization Technology in Jilin Province, Changchun, 130000, Jilin, China
  • 2 Faculty of Science and Marine Environment, University Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, China
  • 3 Key Laboratory of Straw Biology and Utilization, Ministry of Education, College of Life Science, JiLin Agricultural University, Changchun, 130000, JiLin, China; Innovation Platform of Straw Comprehensive Utilization Technology in Jilin Province, Changchun, 130000, Jilin, China. Electronic address: sun_jlau@163.com
Environ Res, 2020 09;188:109829.
PMID: 32798948 DOI: 10.1016/j.envres.2020.109829

Abstract

Intensive studies have been performed on the improvement of bioethanol production by transformation of lignocellulose biomass. In this study, the digestibility of corn stover was dramatically improved by using laccase immobilized on Cu2+ modified recyclable magnetite nanoparticles, Fe3O4-NH2. After digestion, the laccase was efficiently separated from slurry. The degradation rate of lignin reached 40.76%, and the subsequent cellulose conversion rate 38.37% for 72 h at 35 °C with cellulase at 50 U g-1 of corn stover. Compared to those of free and inactivated mode, the immobilized laccase pre-treatment increased subsequent cellulose conversion rates by 23.98% and 23.34%, respectively. Moreover, the reusability of immobilized laccase activity remained 50% after 6 cycles. The storage and thermal stability of the fixed laccase enhanced by 70% and 24.1% compared to those of free laccase at 65 °C, pH 4.5, respectively. At pH 10.5, it exhibited 16.3% more activities than its free mode at 35 °C. Our study provides a new avenue for improving the production of bioethanol with immobilized laccase for delignification using corn stover as the starting material.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.