Fulltext

Multi-Laboratory Evaluation of a Lateral Flow Rapid Test for Detection of Amebic Liver Abscess

Noordin R 1 , Yunus MH 1 , Saidin S 2 , Mohamed Z 3 , Fuentes Corripio I 4 , Rubio JM 4 Show all authors , Golkar M 5 , Hisam S 6 , Lee R 7 , Mahmud R 8

Affiliations 

  • 1 Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang, Malaysia
  • 2 Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, Perak, Malaysia
  • 3 Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia
  • 4 Parasitology Department, National Microbiology Centre, Instituto de Salud Carlos III, Madrid, Spain
  • 5 Parasitology Department, Pasteur Institute of Iran, Tehran, Iran
  • 6 Parasitology Unit, Institute for Medical Research, NIH Complex Setia Alam, MOH Malaysia, Selangor, Malaysia
  • 7 NSW Health Pathology, Centre for Infectious Disease and Microbiology Laboratory Services, Westmead Hospital, Westmead, Australia
  • 8 Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
Am J Trop Med Hyg, 2020 12;103(6):2233-2238.
PMID: 32996457 DOI: 10.4269/ajtmh.20-0348

Abstract

Independent evaluations of XEh Rapid®, an IgG4-based rapid dipstick test, were performed to assess its diagnostic performance to detect amebic liver abscess (ALA) using 405 samples at seven laboratories in four countries. The test showed high diagnostic specificity (97-100%) when tested with samples from healthy individuals (n = 100) and patients with other diseases (n = 151). The diagnostic sensitivity was tested with a total of 154 samples, and the results were variable. It was high in three laboratories (89-94%), and moderate (72%) and low (38%) in two other laboratories. Challenges and issues faced in the evaluation process are discussed. Nevertheless, XEh Rapid is promising to be developed into a point-of-care test in particular for resource-limited settings, and thus merits further confirmation of its diagnostic sensitivity.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

MeSH terms
Similar publications