Affiliations 

  • 1 College of Tobacco Science, Henan Agricultural University, Zhengzhou, 450002, China; Henan Biochar Engineering Technology Research Center, 450002, China
  • 2 College of Tobacco Science, Henan Agricultural University, Zhengzhou, 450002, China
  • 3 Pyrolysis Technology Research Group, Institute of Tropical Aquaculture and Fisheries (Akuatrop), Universiti Malaysia Terengganu, 21030, Kuala Terengganu, Terengganu, Malaysia
  • 4 Nanping Branch, Fujian Tobacco Sciences Research Institute, Nanping, 353000, China
  • 5 College of Resources and Environment, Henan Agricultural University, Zhengzhou, 450002, China
  • 6 College of Resources and Environment, Henan Agricultural University, Zhengzhou, 450002, China. Electronic address: lilantao@henau.edu.cn
  • 7 Pyrolysis Technology Research Group, Institute of Tropical Aquaculture and Fisheries (Akuatrop), Universiti Malaysia Terengganu, 21030, Kuala Terengganu, Terengganu, Malaysia; Henan Province Engineering Research Center for Biomass Value-added Products, School of Forestry, Henan Agricultural University, Zhengzhou, 450002, China. Electronic address: lam@umt.edu.my
Environ Res, 2021 Jan;192:110273.
PMID: 33002505 DOI: 10.1016/j.envres.2020.110273

Abstract

Pot experiments were conducted to investigate the influence of biochar addition and the mechanisms that alleviate Cd stress in the growth of tobacco plant. Cadmium showed an inhibitory effect on tobacco growth at different post-transplantation times, and this increased with the increase in soil Cd concentration. The growth index decreased by more than 10%, and the photosynthetic pigment and photosynthetic characteristics of the tobacco leaf were significantly reduced, and the antioxidant enzyme activity was enhanced. Application of biochar effectively alleviated the inhibitory effect of Cd on tobacco growth, and the alleviation effect of treatments is more significant to the plants with a higher Cd concentration. The contents of chlorophyll a, chlorophyll b, and carotenoids in the leaves of tobacco plants treated with biochar increased by 9.99%, 12.58%, and 10.32%, respectively, after 60 days of transplantation. The photosynthetic characteristics index of the net photosynthetic rate increased by 11.48%, stomatal conductance increased by 11.44%, and intercellular carbon dioxide concentration decreased to 0.92. Based on the treatments, during the growth period, the antioxidant enzyme activities of tobacco leaves comprising catalase, peroxidase, superoxide dismutase, and malondialdehyde increased by 7.62%, 10.41%, 10.58%, and 12.57%, respectively, after the application of biochar. Our results show that biochar containing functional groups can effectively reduce the effect of Cd stress by intensifying the adsorption or passivation of Cd in the soil, thereby, significantly reducing the Cd content in plant leaves, and providing a theoretical basis and method to alleviate soil Cd pollution and effect soil remediation.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.