Affiliations 

  • 1 Centre for Chemical Sciences and Technology, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, Telangana, 500085, India
  • 2 Medicinal Chemistry Division, GVK Biosciences Private Limited, Plot No. 28A, IDA Nacharam, Hyderabad, Telangana, 500076, India. rajeshbagepallimadhu@gmail.com
  • 3 Centre for Chemical Sciences and Technology, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, Telangana, 500085, India. jayashreeanireddy@gmail.com
  • 4 Department of Biomedical Sciences and Therapeutics, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, 88400, Kota Kinabalu, Sabah, Malaysia. pvrao@ums.edu.my
  • 5 Chemical Engineering Institute, Ural Federal University, Yekaterinburg, Russian Federation, 620002. vijaykumarreddy.jntuh@gmail.com
  • 6 Medicinal Chemistry Division, GVK Biosciences Private Limited, Plot No. 28A, IDA Nacharam, Hyderabad, Telangana, 500076, India
  • 7 Drug Discovery Research Laboratory, Department of Pharmacy, Birla Institute of Technology and Science - Pilani, Hyderabad Campus, Hyderabad, Telangana, 500078, India
  • 8 Chemical Engineering Institute, Ural Federal University, Yekaterinburg, Russian Federation, 620002
Sci Rep, 2020 11 26;10(1):20720.
PMID: 33244007 DOI: 10.1038/s41598-020-77590-1

Abstract

Acridone based synthetic and natural products with inherent anticancer activity advancing the research and generating a large number of structurally diversified compounds. In this sequence we have designed, synthesized a series of tetracyclic acridones with amide framework viz., 3-(alkyloyl/ aryloyl/ heteroaryloyl/ heteroaryl)-2,3-dihydropyrazino[3,2,1-de]acridin-7(1H)-ones and screened for their in vitro anti-cancer activity. The in vitro study revealed that compounds with cyclopropyl-acetyl, benzoyl, p-hydroxybenzoyl, p-(trifluoromethyl)benzoyl, p-fluorobenzoyl, m-fluorobenzoyl, picolinoyl, 6-methylpicolinoyl and 3-nicotinoyl groups are active against HT29, MDAMB231 and HEK293T cancer cell lines. The molecular docking studies performed for them against 4N5Y, HT29 and 2VWD revealed the potential ligand-protein binding interactions among the neutral aminoacid of the enzymes and carbonyl groups of the title compounds with a binding energy ranging from - 8.1394 to - 6.9915 kcal/mol. In addition, the BSA protein binding assay performed for them has confirmed their interaction with target proteins through strong binding to BSA macromolecule. The additional studies like ADMET, QSAR, bioactivity scores, drug properties and toxicity risks ascertained them as newer drug candidates. This study had added a new collection of piperazino fused acridone derivatives to the existing array of other nitrogen heterocyclic fused acridone derivatives as anticancer agents.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.