• 1 Department of Chemical Engineering, Khalifa University, P.O. Box 127788, Abu Dhabi, United Arab Emirates
  • 2 Department of Chemical Engineering, Loughborough University, Loughborough LE11 3TU, Leicestershire, UK
  • 3 Department of Chemical Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, 43500 Selangor Darul Ehsan, Malaysia. Electronic address:
Bioresour Technol, 2021 Jan;319:124243.
PMID: 33254466 DOI: 10.1016/j.biortech.2020.124243


This study highlights biohydrogen production enrichment through NiO and CoO nanoparticles (NPs) inclusion to dark fermentation of rice mill wastewater using Clostridium beijerinckii DSM 791. NiO (~26 nm) and CoO (~50 nm) NPs were intrinsically prepared via facile hydrothermal method with polyhedral morphology and high purity. Dosage dependency studies revealed the maximum biohydrogen production characteristics for 1.5 mg/L concentration of both NPs. Biohydrogen yield was improved by 2.09 and 1.9 folds higher for optimum dosage of NiO and CoO respectively, compared to control run without NPs. Co-metabolites analysis confirmed the biohydrogen production through acetate and butyrate pathways. Maximum COD reduction efficiencies of 77.6% and 69.5% were observed for NiO and CoO inclusions respectively, which were higher than control run (57.5%). Gompertz kinetic model fitted well with experimental data of NPs assisted fermentation. Thus, NiO and CoO inclusions to wastewater fermentation seems to be a promising technique for augmented biohydrogen production.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.