• 1 Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, Buraidah 52571, Saudi Arabia
  • 2 Department of Pharmacology, Amity Institute of Pharmacy, Amity University Haryana, Gurugram-122413, India
  • 3 Department of Pharmacology and Toxicology, College of Pharmacy, Qassim University, Buraidah 51452, Saudi Arabia
  • 4 Department of Dental Hygiene, College of Applied Health Sciences in Alrass, Qassim University, Alrass region 51921, Saudi Arabia
  • 5 School of Pharmacy, Faculty of Health and Medical Sciences, Taylor's University, Subang Jaya, Kuala Lumpur 47500, Malaysia
  • 6 Department of Pharmacology, Faculty of Medicine, AIMST University, Semeling 08100, Kedah, Malaysia
  • 7 Department of Pharmaceutics, Buraydah College of Dentistry and Pharmacy, Buraydah 51418, Saudi Arabia
Saudi J Biol Sci, 2021 Oct;28(10):5579-5584.
PMID: 34588868 DOI: 10.1016/j.sjbs.2021.05.072


The current study primarily focused on the pharmacognostical and phytochemical screening of Canna indica and further analyzing the leaves extract for toxicological profile and neuroprotective potential. The microscopic, dry powder properties of the leaf material and phytochemical, physicochemical analysis was evaluated for pharmacognostical assessment. Dry leaves of C. indica were extracted using methanol and then further studied for both in vitro and in vivo toxicological study. The acute toxicity was measured by estimating the antioxidant defense system and anatomical impairment in the rat's organs. Also, the neuroprotective activity of the plant extract was assessed using anticholinesterase enzymatic inhibitory assay. The extract was found to be hemocompatible and showed absences of induction of behavioural changes. Likewise, no changes were seen on the anatomical structure of the rat's organs. The methanolic extract portrayed a significant upsurge in the reduced glutathione level and showed a comparable acetylcholinesterase inhibition in a dosedependent manner with an IC50 value of 14.53 μg/mL compared to the standard Donepezil with an IC50 value of 13.31 μg/mL. C. indica has compelling pharmacognostical characteristics, good safety reports, and significant antioxidant as well as the neuroprotective potential that shows great potential for its further in-depth research for pharmacological use.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.