The objective of this study was to investigate the degree to which Auditory Steady State Response (ASSR) thresholds correlate with behavioral thresholds in two groups of adult subjects, one with normal hearing and the other with sensorineural hearing impairment. When the relationship between ASSR and behavioral thresholds were analyzed separately according to different groups of subjects, significant correlations were only found for the hearing impaired group. The mean differences between the actual and the predicted thresholds derived from linear regression analysis for that group of subjects were found to be 5 dB (SD = 4), 3 dB (SD = 3), 4 dB (SD = 3) and 4 dB (SD = 4) with correlation coefficients of 0.80, 0.88, 0.91 and 0.97 for the 500, 1000, 2000 and 4000 Hz carrier frequencies, respectively. When the relationship between ASSR and behavioral thresholds were analyzed using data from both groups of subjects, correlation coefficients were found to be higher across carrier frequencies of 500 to 4000 Hz (r ³ 0.96) with mean differences between the actual and the predicted thresholds of 6 dB (SD = 3), 4 dB (SD = 3), 4 dB (SD = 3) and 6 dB (SD = 3) for the hearing impaired group and 11dB (SD = 7), 8 dB (SD = 8), 8 dB (SD = 6) and 10 dB (SD = 7) for the normal hearing group. However, it was observed that the range of differences between the actual and the predicted thresholds were quite large reaching 34 dB for the 500 and 4000 Hz carrier frequencies. This suggests that in clinical setting, ASSR cannot predict the presence or absence of a hearing loss accurately. In general, it can be concluded that ASSR allow for an accurate prediction of behavioral thresholds within ± 10 dB in subjects with hearing impairment. However, ASSR cannot accurately predict hearing thresholds in normally hearing individuals.
Key words: auditory steady-state response threshold, behavioral threshold, adult, normal hearing, hearing impairment
The abdominal pelvic cavity has always been regarded as one of the most vulnerable regions of the body. Injuries to this part have been known to be very serious. Based on this a retrospective study was performed on postmortem cases with abdominal and pelvic trauma from 1 October 2002 to 31 January 2003 at the Forensic Institute, Hospital Kuala Lumpur to determine the common pattern of abdomino-pelvic injuries, the injury’s relationship to epidemiological and socio-economic factors, risk organ within the abdominopelvic region and relationship between injuries and survival period. A total of 122 cases abdomino-pelvic were obtained stemming from medico legal postmortems procedures. Results indicated that those aged between 21 to 30 years (38.5%) had the highest incidence of this type of injury. Categorization based on gender showed that males (90.2%) dominated most of the cases. Prevalence based on socio-economic status showed that lower socioeconomy class (52.5%) had the highest incident. This was followed by the middle income (39.3%) and finally the higher income group. The most numerous type of trauma was non-penetrating trauma (94.3%). It was also found that victims with low injury severity score (ISS) had a longer survival period as compared to those with high ISS. It was also noted that victims with two or more region injuries either were spot dead or brought dead. The most common combination of injuries was abdomen, pelvic, chest and limb. In most blunt trauma cases majority of victims had one or two organ involvement while penetrating injuries were difficult to asses. The most common organ involved in this type of trauma was liver (72.1%), pelvic bone (47.5%) and spleen (43.4%). In conclusion, abdomino-pelvic injury is a serious condition and should not be overlooked. Proper attention towards their diagnosis and management is important and so any patient of head injury with coma must be considered as having intra-abdominal injury until proven otherwise.
The relationship between anaemia and cognitive function was evaluated among 35 Chinese elderly (24 men and 11 women) aged 60 to 85 years (mean age 70.1 ± 6.7 years) from five old folks homes in Klang Valley. They were interviewed to obtain information on social and health status, habitual dietary intake and cognitive function. Hodkinson's Abbreviated Mental Test was used to measure the cognitive function. Haematological indices which included Full Blood Count (FBC), serum iron, serum ferritin, Total Iron Binding Capacity (TIBC), serum folate and serum cobalamine (vitamin B12) were measured using an automated analyzer. Anthropometric measurements and clinical signs of anaemia were also examined. The findings indicated that the prevalence of anaemia as assessed using haemoglobin alone was 22.9%, while iron deficiency anaemia based on low serum iron, microcytic and hypochromic criterion was detected among 5.7% of the sample. Subclinical folate and vitamin B12 deficiencies were diagnosed among 34.3% and 8.6% of the subjects. However, there was no occurrence of megaloblastic anaemia. There was a positive correlation between cognitive score with mid upper arm circumference (MUAC) (r=0.547, p
Kajian garis pangkal pengimejan resonans magnet kefungsian (fMRI) telah dijalankan ke atas 2 orang subjek lelaki sihat (kidal dan tidak kidal) masing-masing berumur 22 dan 25 tahun. Imbasan fMRI dijalankan menggunakan sistem pengimejan resonans magnet (MRI) 1.5 T di Jabatan Radiologi, Hospital Universiti Kebangsaan Malaysia. Kajian ini menggunakan gerakanjari tangan kanan dan kiri untuk merangsang aktiviti neuron di dalam korteks serebrum. Paradigma 5 kitar aktifIrehat digunakan dengan setiap kitar mengandungi satu blok aktif dan satu blok rehat yang masing-masing mengandungi 10 siri pengukuran. Imej fMRI dianalisis menggunakan pekej perisian MatLab dan pemetaan statistik berparameter 2 (sPM2). Proses pendaftaran jasad tegar menggunakan penjelmaan afin 6 parameter dilakukan ke atas kesemua imej kefungsian berwajaran T2*. Keputusan menunjukkan bahawa pergerakan subjek adalah minimum sama ada dalam arah translasi (< 1 mm) atau putaran (< 1 ). Kesemua imej dinormalkan melalui proses peledingan tak linear menggunakan penjelmaan afin 12 parameter dan didapati sepadan dengan pencontoh yang telahpun mematuhi ruang anatomi piawai. Walau bagaimanapun, bentuk, resolusi dan kontras imej kefungsian telah berubah sedikit berbanding dengan imej asal. Pelicinan imej menggunakan kernel Gaussian isotropik 6 mm menyebabkan data imej lebih bersifat parametrik dengan kehilangan yang ketara dalam resolusi dan kontras. Pengasingan struktur yang dilakukan ke atas imej berwajaran T1 mengklaskan tisu otak kepadajirim kelabu, jirim putih dan bendalir serebrospina. Pasca pemprosesan ruang bagi imej kefungsian dan struktur menjadikan data imej bersifat parametrik dengan taburan jenis Gaussian dan sedia untuk dianalisis menggunakan model linear am dan teori medan rawak Gaussian.
Bawang merah merupakan tumbuhan yang mengandungi pelbagai khasiat dan telah dijadikan sebagai makanan dan
ubatan sejak dahulu lagi oleh pelbagai kaum dan bangsa. Kajian ini dilakukan bagi mengenal pasti khasiat bawang
merah terhadap tubuh manusia dari perspektif sarjana-sarjana perubatan Islam dan kajian-kajian saintifi k. Data-data
literatur telah dikumpulkan dari empat buah buku perubatan Islam dan 14 artikel daripada pelbagai jurnal berkaitan.
Data terkumpul dianalisis menggunakan Nvivo10.0 bagi membentuk kategori bersesuaian seterusnya menghasilkan
dapatan kajian. Hasil kajian menunjukkan terdapat sekurang-kurangnya 17 khasiat bawang merah yang merangkumi
enam khasiat dari perspektif sarjana perubatan Islam seperti merawat penyakit mata sentiasa berair, merawat masalah
dalam telinga, merawat jangkitan dari gigitan anjing, meneutralkan racun, merangsang syahwat dan merawat penyakit
akibat air tercemar. Manakala 11 manfaat lagi dikenal pasti daripada kajian-kajian saintifi k seperti ia merupakan agen
antiplatelet, agen anti-pembentukan lemak, agen antioksidan, agen antitrombotik, agen antikarsinogen, agen antidepresen/
antimurung, agen antiinfl amasi, agen antiasma, agen antibiotik, menurunkan tahap hiperglisemia dan mengurangkan
risiko penyakit aterosklerosis. Justeru kajian ini mencadangkan agar bawang merah diketengahkan sebagai salah satu
rawatan organik serta alternatif kepada rawatan konvensional sedia ada.
Hibiscus sabdariffa Linn. (roselle) is a polyphenol rich fruit. This study aimed to identify the neuroprotective effect of
roselle on LPS-induced cell proliferation and nitric oxide-induced free radical in microglia and neuroblastoma cells.
MTT assay was used to identify the appropriate concentration of roselle and LPS for microglia and neuroblastoma cells
proliferation study. Griess assay were used to determine the level of nitric oxide accumulated based on the reaction of
Griess to estimate the activity of iNOS in nitric oxide production. The results showed that roselle at the concentration of
50 μg/mL and 100 μg/mL and LPS at concentration of 1 μg/mL does not give cytotoxic effect towards microglia C8-B4 and
neuroblastoma LN18 cells. The roselle treatment at 50 μg/mL and 100 μg/mL showed a protective effect on LPS-induced
microglia C8-B4 cells. However, in neuroblastoma LN18 cells, no protective effect was seen on both 50 μg/mL and 100
μg/mL of roselle treatment following induction with 1 μg/mL of LPS. On the other hand, the production of nitric oxide
(NO) was reduced when LPS-induced microglia C8-B4 cells were treated with 50 μg/mL of roselle. Treatment of roselle
at concentration 100 μg/mL on LPS-induced neuroblastoma LN18 cells also reduced the production of nitric oxide. As a
conclusion, roselle had the ability to give neuroprotective effect by the inhibition of LPS induction activity on microglia
activation for normal and cancer cells at different concentrations
Two species of plants, Andrographis paniculata and Euphorbia hirta were screened for antibacterial activities against three Gram positives and Gram negatives. The leaves from both plants were extracted by methanol extraction. The antibacterial activity was detected with spread plate well diffusion method. The extracts of both plants demonstrated inhibitory activity against both Gram negative and positives bacteria. Staphylococcus aureus, Bacillus subtilis, Streptococcus epidemidis, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa. The minimum inhibitory concentration (MIC determination using micro dilution method showed that the A/tic of A. paniculata for the tested bacteria were 1.56 mg/ml (Staph. aureus), 3.13 mg/ml (Bacillus subtilis), 3.13 mg/ml (Strept. epidemidis), 1.56 mg/ml (Escherichia cob), 12.50 mg/ml (Klebsiella pneumoniae) and 3.13 mg/ml (Pseudomonas aeruginosa) respectively. The MIC value for E. hirta was 6.25 mg/ml (Staph. aureus) and 3.13 mg/ml (Bacillus subtilis), 3.13 mg/ml (Strept. Epidemidis), 3.13 mg/ml (Pseudomonas aeruginosa),12.5 mg/ml (Escherichia coli), and 6.25 mg/ml (Klebsiella pneumoniae). Both plants represent a potential for pharmaceutical and agricultural applications and are worthy of further study.
Natural peptides in insect vectors played an important role in the control of
pathogens. Musca domestica Linnaeus and Chrysomya megacephala Fabricius were
two species of local fly chosen to detect presence of antimicrobial peptide substance.
The screening of the antimicrobial activity was carried using a spectrophotometric
method. Results were obtained much quicker and less laborious. The results showed
larva hemolymph of M. domestica lysed Bacillus subtilis and two Gram negatives,
Escherichia coli and Pseudomona. aeruginosa. The pupae hemolymph only lysed E.
coli. Whereas, the hemolymph of C. megacephala larva showed bactericidal effect
against both of the Gram positives tested, i.e. B. subtilis and Staph. aureus. and no
effect was against the Gram negatives. The pupa showed lytic activity against Staph.
aureus and P. aeruginosa. As a conclusion, the larva and pupa hemolymph of M.
domestica and C. megacephala demonstrated antibacterial activity. However, larva
hemolymph of M. domestica and C. megacephala has broader antibacterial activity
against both Gram positive and negative bacteria.
The leaf and bark oils of Cinnamomum verum J.S. Presl. were examined for their antifungal activity against 6 dermatophytes (Trichophyton rubrum, T. mentagrophytes, T. tonsurans, Microsporum canis, M. gypseum and M. audouini), one filamentous fungi (Aspergillus fumigatus) and 5 strains of yeasts (Candida albicans, Ca. glabrata, Ca. tropicalis, Ca. parapsilosis and Crytococcus neoformans) by using the broth microdilution method. The antifungal activities of 4 standard compounds (cinnamaldehyde, eugenol, linalool and a-terpineol) which were major constituents in the oils were also investigated in an effort to correlate the effectiveness of the oils with those of the components of the oils. The combined antifungal effect of the oils against M. canis, M. gypseum and Cr. neoformans was investigated by the checkerboard assay. Isobolograms were constructed and Fractional Inhibitory Concentrations Index (FICI) were calculated to determine the combination effects between the oils. The chemical composition of the oils was analyzed by gas chromatography (GC) and gas chromatography- mass spectrometry (GC-MS). The oils showed strong activity against all the tested fungi with Minimum Inhibition Concentration (MIC) values ranging from 0.04 to 0.31 mg/ml. Cinnamaldehyde which was the most abundant component of the bark oil of C. verum showed the strongest activity against all the fungi studied. Based on the results of the assay on standard samples, it may be that the high levels of cinnamaldehyde and eugenol in the oils and in combination with the minor components could be responsible for the high antifungal activity of the oils. The antifungal effect of the leaf and bark oils of C. verum in combination against the tested fungi was not synergistic. However, the effect was additive against M. gypseum and antagonistic against Cr. neoformans and M. canis.
Hypoglycaemic and antihyperglycemic activity of oil palm Elaeis guineensis fruit extract on normal and Streptozotocininduced
diabetic rats was studied. The oil palm fruit extract (OPF) were administered orally at different concentrations (100,
200 and 500 mg kg-1 b.w.) in fasting and post-prandial rats. Hypoglycaemia was not observed in the group of normal rats
treated with OPF. In fasting rats, OPF (500 mg kg-1 b.w.) has caused the blood glucose level (BGL) to reduce significantly.
For post-prandial diabetic rats, the antihyperglycemic activity was observed after OPF treatment at concentrations 200
and 500 mg kg-1. Chronic OPF treatments (for 28 days) had increased the diabetic rat’s body weight and reduced BGL as
well as improved plasma insulin secretion. The result of this study suggests E. guineensis palm fruit extract show evidence
of antihyperglycemic properties from the reduction of the BGL in diabetic rats.
Ficus deltoidea or Mas cotek is one of the common medicinal plants used in Malaysia has been claimed to have antidiabetic activity. However, scientific evidence to confirm its efficacy is still lacking. Thus, the present study was undertaken to evaluate the potential of ethanolic extract of Ficus deltoidea to reduce hyperglycaemia in streptozotocininduced diabetic rats at different prandial state. The results showed that, ethanolic extract of Ficus deltoidea significantly reduced fasting and postprandial hyperglycemia particularly after 4 and 6 hours of extract administration. Likewise, glucose tolerance activity was significantly improved in the presence of Ficus deltoidea ethanolic extract at a low dose, 100 mg/kg. It is suggested that ethanolic extract of Ficus deltoidea at particular doses, possess fasting and postprandial antihyperglycemic activity as well as glucose tolerance activity in streptozotocin-induced diabetic rats.
The aqueous and ethanol extracts of Stichopus chloronotus Brandt were investigated for their effectiveness against guinea pig dermatophytosis caused by Microsporum canis and Trichophyton mentagrophytes using the hair root invasion test. The ethanol extract at 10 mg/ml showed 82.8 % efficacy against T. mentagrophytes while the aqueous extract at similar concentration showed 84.8% efficacy against M. canis infection, as compared to econazole which showed 100% efficacy against both infections. No adverse effect on the skin was observed in the treated animals. In conclusion, aqueous and ethanol extracts of S. chloronotus showed high antimycotic activity against experimentally induced dermatophytosis in guinea pigs.
Neuroplasticity has been subjected to a great deal of research in the last century. Recently, significant emphasis has been
placed on the global effect of localized plastic changes throughout the central nervous system, and on how these changes
integrate in a pathological context. The present study aimed to demonstrate the functional cortical reorganization before
and after surgery using magnetoencephalography (MEG) in a participant with brain tumor. Results of Visual Evoked
Magnetic Field (VEF) based on functional MEG study revealed significantly different of MEG N100 waveforms before and
after surgery. Larger and additional new locations for visual activation areas after the surgery were found suggesting
neuroplasticity. The present study highlight a physiological plasticity in a teenage brain and the alterations regarding
neural plasticity and network remodeling described in pathological contexts in higher-order visual association areas.
This study was conducted to evaluate the oxidative damage in diabetic mellitus induced rats. The evaluation of DNA damage was carried out by the Alkaline Comet Assay using peripheral lymphocyte cells taken from streptozotocin-induced diabetic rats (50 mg/kg) and control rats. The levels of malondealdehyde (MDA), 4-hydroxynonenal (4-HNE), fasting blood glucose (FBG) and HbA1c were also measured. All the induced diabetic rats were hyperglycemic until the end of the study with significantly higher levels of FBG and HbA1c as compared to the control rats. The results showed the percentage of tail DNA and tail moment values were also significantly higher in the diabetic induced rats. The same observations were made on the levels of plasma MDA and 4-HNE. In conclusion, this study indicated that hyperglycemic condition in diabetic induced rats could generate oxidative DNA damage.
ABSTRACT
Clinacanthus nutans (C. nutans) leaf extracts have been widely used by cancer patients in Malaysia and local practice claims a cure to cancer. There were several studies done to determine the cytotoxicity potency of C. nutans extracts on various types of cells. However, there is still lacking on the knowledge regarding the combination effect of C. nutans with anticancer drugs. Thus, the study was carried out to determine the cytotoxicity potency of C. nutans extracts and paclitaxel (PTX) alone and, in combination on MDA-MB-231 cells. The cells were treated with 100% ethanol extract of C. nutans (CNE) and water extract of C. nutans (CNA), PTX and combination of both extracts and PTX for 72 hours and the cytotoxic activity was determined using SRB assay. Result showed that CNE had little cytotoxic activity, whereas CNA showed no cytotoxic activity on MDA-MB-231 cells. For combination treatment of C. nutans extracts and PTX, only CNE showed significant enhanced PTX-induced cytotoxicity (p < 0.05), meanwhile CNA inhibited PTX-induced cytotoxicity significantly (p < 0.05). As a conclusion, CNE was able to increase PTX potency to inhibit the viability of MDA-MB-231 cells.
Aging is associated with increased risk of frailty and malnutrition. However, food insecurity has rarely been highlighted
in the elderly population, especially among the low income group. Thus, a cross-sectional study was conducted to
determine the association between nutritional status, food insecurity and frailty among elderly in low income residences
in Klang Valley. A total of 72 elderly individuals aged 60 years and above was selected (mean age 66 ± 6 years) through
convenient sampling. Participants were interviewed to obtain information on socio-demographic, health status, food
insecurity and cognitive status. Anthropometrics parameters and frailty assessments was measured using standard
criteria. Results showed that 75.0% of the participants had abdominal obesity. Nearly half of the participants were
overweight (41.7%), followed by normal (43.0%) and underweight (15.3%). With respect to food insecurity, most of them
reported that they had enough food (93.1%). There were significant correlation (p < 0.05) between food insecurity with
height (r = -0.263, p = 0.026). Most of the participants were pre-frail (58.3%), frail (27.8%) and followed by non-frail
(13.9%). Calcium intake is inversely associated with frailty (t = -2.62, p = 0.011). In conclusion, food insecurity was not
a problem, however, half of the subjects were overweight and pre-frail. Three out four subjects had abdominal obesity.
There is a need to investigate further the pathogenesis of fat frail in this low income elderly population and formulate
effective intervention strategies.
A preliminary study was conducted to determine the level of oxidative DNA damage, fruits and vegetables intake among 50 breast cancer patients (cases) as compared to 50 healthy women (controls) with no known medical history of breast cancer in Klang Valley. Both groups were matched for age and ethnicity. Data on socio-demographic, health status and medical history, fruits and vegetables intake, and supplements intake were obtained through an interviewbased questionnaire. Anthropometry measurements included weight, height, and waist and hip circumference were also carried out on subjects. A total of 3mL fasting venous blood was drawn to assess lymphocytes oxidative DNA damage using Alkaline Comet Assay. Results indicated that the mean intake of fruits and vegetables was lower in cases (4.09 ± 1.17 servings/d) than controls (4.77 ± 0.90 servings/d)(p < 0.05) The intake of fruits and vegetables from family groups of solanaceae, myrtaceae, caricaceae, apiaceae, brinjal, rutaceae, broccoli, orange, carrot, watermelon were 0.5 - 1 servings/week significantly higher among controls as compared to cases (p < 0.05 for all parameters). However, the intake of fruits from rosaceae family and apple was higher among controls than cases (p < 0.05). The estimated intake of ß-carotene, carotenoids, vitamin A, vitamin C (p < 0.001), a-carotene and lycopene (p < 0.05) from fruits and vegetables were higher among controls than cases. Mean DNA damage level of cases (4.55 ± 1.78 % DNA in tail, %TD; 0.35 ± 0.21 tail moment, TM) were 3.5 and 3.9 times higher than the value of controls (1.3 ± 0.70% TD; 0.09 ± 0.09 TM) (p < 0.001) and the damage increased with higher values of waist hip ratio (% TD, r = 0.396, p < 0.05; TM, r = 0.349, p < 0.05) and waist circumference (% TD, r = 0.334, p < 0.05; TM, r = 0.360, p < 0.05). There was an inverse relationship between oxidative DNA damage with intake of total fruits and vegetables, cauliflowers and water convolvulus and also consumption from rutaceae and solanaceae families. Similar trend was noted for estimated intake of vitamin A, carotenoids, vitamin C, ß-carotene and lycopene. In conclusion, the intake of fruits and vegetables of five servings/d and the consumption of specific families and types of fruits and vegetables might protect against oxidative DNA damage and further reduce breast cancer risk.
Childhood obesity is a global epidemic, which leads to the increasing number of studies on genetic locations associated with obesity-related traits. Polymorphisms of insulin (INS) gene have been shown to be associated with obesity-related phenotypes in Europeans; while insulin receptor (INSR) gene has been associated with energy regulation. Therefore, this study was conducted to investigate the association between the INS (rs689) and INSR (rs3745551) gene polymorphisms with childhood obesity risk in a Malay childhood population. Normal weight (538) and overweight or obese (557) children aged 6-12 years old were genotyped using semi-automated Sequenom iPLEX® Gold. Body mass index (BMI) was calculated from measured body weight and height. The rs689 (T/T: 0.006, A/T: 0.159 and A/A: 0.835) and rs3745551 (G/G: 0.054, A/G: 0.378 and A/A: 0.568) genotype distributions were consistent with Hardy Weinberg equilibrium. The T-minor allele frequency for rs689 was 8.6% and G-minor allele frequency for rs3745551 was 24.3%. Minor allele of INS gene polymorphisms significantly increased risk of obesity among Malay children (sex- and age-adjusted
OR=1.580; 95%CI: 1.134-2.201). However, INSR gene polymorphisms were not significantly associated with childhood obesity. In conclusion, the polymorphisms of INS gene, rather than INSR gene, were associated with childhood obesity in the Malay population.
Loss of teeth can affect masticatory efficiency in older adults. This may result in avoidance or modifications in food
choices and lead to lower intake of important nutrients among older individuals. The aim of this study was to determine
the association between tooth loss and body mass index, BMI, among older adults in Kuala Pilah, Negeri Sembilan. A
cross-sectional study was carried out on 428 older adults aged 50 years and above from selected villages in district of
Kuala Pilah. Respondents were interviewed to collect information on their demographic characteristics. Number of tooth
loss was determined through oral assessment, followed by anthropometric assessment to calculate the BMI of respondents.
Findings showed that majority of the respondents were overweight and obese, 40.4% and 19.9% respectively, while only
a small proportion was underweight, 3.9%. The proportion of edentulism (total tooth loss) was 18.3% and majority of
the older adults had lost more than 12 teeth (77.1%) and less than 4 pairs of occluding posterior teeth (86.0%). Total
and partial tooth loss was found to be not significantly associated with BMI. In relation to the arrangement of teeth,
older adults with reduced number of posterior occluding pairs of teeth were more likely to experience unsatisfactory
BMI (OR = 3.61, 95% CI: 1.48, 8.76). This may suggest that functional arrangement of the tooth in the oral cavity is more
important than the number of tooth loss alone. Thus, maintaining an optimum number of teeth for chewing is essential
for maintenance of ideal BMI.
Daily food intake of women may affect their bone health by altering their bone mineral density (BMD) as the lack of certain
nutrients may affect bone integrity whilst, BMD also can be a predictor of breast cancer. To date, many studies have been
conducted to discuss on association of BMD and mammographic breast density (MBD) and how both are related to breast
cancer risks but no consideration has been made on dietary intake. Therefore, this study was designed to determine
the association of dietary intake with BMD and other breast cancer risk factors. A cross-sectional study on 76 pre- and
postmenopausal women above 40 years underwent mammogram screening and Dual Energy X-ray Absorptiometry (DEXA)
was conducted in Hospital Kuala Lumpur (HKL) for the duration of 1 year. Purposive sampling method was used to choose
the respondents. Women who are diagnosed with breast cancer and underwent cancer treatment were excluded from this
study. DEXA unit (Hologic Discovery W, Hologic, Inc) were used to measure BMD at the femoral neck and lumbar spine in
grams per centimetre squared (g/cm2
) and they were classified into normal and abnormal group based on the T-scores.
The subjects were asked about their daily dietary pattern for a duration of three days using Diet History Questionnaire
(DHQ). The mean of selected characteristics were compared between groups. Additionally, binary logistic regression was
used to determine the association between diet intake with BMD and other risk factors of breast cancer. The total number
of pre- and postmenopausal women who consented to participate in this study are equal. The mean age was 47.1 years
and 54.9 years for premenopausal and postmenopausal women respectively. The results indicate only menopausal age of
the women was statistically significant (p < 0.05). A number of 17% premenopausal and 9% of postmenopausal women
showed to have family history of breast cancer, however, it was not statistically significant (p = 0.12). There was no
significant difference in daily energy intake of food in both groups (p = 0.22). None of the nutrients in daily food intake
showed to be statistically significant. Menstrual status showed an association with BMD with p < 0.05 and the remaining
risk factors did not show any association. Logistic regression revealed that only menstrual status had correlation with
BMD in both groups. This study provided the dietary pattern and the effects on bone health. The association of other risk
factors of breast cancer with BMD were also analysed and most of it showed a negative association.