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Fulltext Larval aggregation on a burned human remain

Kumara TK, Abu Hassan A, Che Salmah MR, Bhupinder S

Trop Biomed, 2012 Mar;29(1):197-9.
PMID: 22543622 MyJurnal

A burned human remain was found outdoor (5º 27' N, 100º 16' E) in Penang Island. The deceased was last seen alive on 23 April 2010 at 2230 h and was found burned on 24 April 2010 at 1920 h. Larval aggregation of second instar Chrysomya megacephala was observed on the chest of the deceased.

Matched MeSH terms: Cadaver*
Fulltext A report on the pupae of Desmometopa sp. (Diptera: Milichiidae) recovered from a human corpse in Malaysia

Kumara TK, Abu Hassan A, Che Salmah MR, Bhupinder S

Trop Biomed, 2010 Apr;27(1):131-3.
PMID: 20562823

The pupae of Desmometopa sp. (Diptera: Milichiidae) were collected from a human corpse found indoor in active decay stage together with the larvae of Sarcophagidae, Synthesiomyia nudiseta (Wulp), Chrysomya megacephala (Fabricius) and Chrysomya rufifacies (Macquart). This research note is the first report of the Desmometopa sp. recovered from a human corpse in Malaysia.

Matched MeSH terms: Cadaver*
Fulltext The infestation of Dermestes ater (De Geer) on a human corpse in Malaysia

Kumara TK, Abu Hassan A, Che Salmah MR, Bhupinder S

Trop Biomed, 2009 Apr;26(1):73-9.
PMID: 19696730 MyJurnal

A human corpse at an advanced stage of decomposition was found in a house in the residential area of Bukit Mertajam, Penang, Malaysia. Entomological specimens were collected during the post-mortem and the live specimens were subsequently reared at room temperature. The time of death was estimated to have been 14 days previous to the discovery of the body based on the police investigation. Both adult and larvae of the beetle Dermestes ater (De Geer) were found to be infesting the corpse and from the stage of decomposition of the body and the estimated time of death it would appear that infestation may have begun at a relatively early stage of decomposition.

Matched MeSH terms: Cadaver*
Fulltext Larval growth of the muscid fly, Synthesiomyia nudiseta (Wulp), a fly of forensic importance, in the indoor fluctuating temperatures of Malaysia

Kumara TK, Abu Hassan A, Che Salmah MR, Bhupinder S

Trop Biomed, 2009 Aug;26(2):200-5.
PMID: 19901906 MyJurnal

Larvae of the Synthesiomyia nudiseta (Wulp) were collected from a decomposed human corpse at the Department of Forensic Medicine, Penang Hospital. A colony of this species was established and the eggs were collected for rearing. The developmental times, rearing temperatures, and relative humidity were recorded twice daily from the time the eggs collected until adult emergence. An average of 5 larvae were randomly collected from the rearings twice daily, warm-water killed and preserved in Kahle's solution. The larval instar stages were determined by observing the number of posterior spiracular slits and the length of the preserved larvae were measured. When the larval life cycle was completed, the accumulated developmental times were calculated. A total of 8 replicates were carried out. The temperature of the rearing room was 28.5+/-1.5 degrees Celcius while the relative humidity was within 67-85%. The total developmental time for S. nudiseta was 322+/-19 hours (13.4+/-0.8 days).

Matched MeSH terms: Cadaver
Comparison between percutaneous fluoroscopic-guided and conventional open pedicle screw placement techniques for the thoracic spine: a safety evaluation in human cadavers

Kwan MK, Chiu CK, Lee CK, Chan CY

Bone Joint J, 2015 Nov;97-B(11):1555-61.
PMID: 26530660 DOI: 10.1302/0301-620X.97B11.35789

Percutaneous placement of pedicle screws is a well-established technique, however, no studies have compared percutaneous and open placement of screws in the thoracic spine. The aim of this cadaveric study was to compare the accuracy and safety of these techniques at the thoracic spinal level. A total of 288 screws were inserted in 16 (eight cadavers, 144 screws in percutaneous and eight cadavers, 144 screws in open). Pedicle perforations and fractures were documented subsequent to wide laminectomy followed by skeletalisation of the vertebrae. The perforations were classified as grade 0: no perforation, grade 1: < 2 mm perforation, grade 2: 2 mm to 4 mm perforation and grade 3: > 4 mm perforation. In the percutaneous group, the perforation rate was 11.1% with 15 (10.4%) grade 1 and one (0.7%) grade 2 perforations. In the open group, the perforation rate was 8.3% (12 screws) and all were grade 1. This difference was not significant (p = 0.45). There were 19 (13.2%) pedicle fractures in the percutaneous group and 21 (14.6%) in the open group (p = 0.73). In summary, the safety of percutaneous fluoroscopy-guided pedicle screw placement in the thoracic spine between T4 and T12 is similar to that of the conventional open technique.

Matched MeSH terms: Cadaver
The Safety and Strength of a Novel Medial, Partial Nonthreaded Pedicle Screw: A Cadaveric and Biomechanical Investigation

Kwan MK, Chan CY, Saw LB, Rukmanikanthan S, Lenke LG

Clin Spine Surg, 2017 04;30(3):E297-E304.
PMID: 28323715 DOI: 10.1097/BSD.0b013e3182aab29d

STUDY DESIGN: Cadaveric and biomechanical study.
OBJECTIVE: The aim of this study was to assess the safety and pullout strength of medial, partial nonthreaded thoracic pedicle screws compared with conventional screws.
SUMMARY OF BACKGROUND DATA: The perforation rate of the pedicle screws has been reported as high as 41%. Nerve injury and irritation can result from the compression of malpositioned screw on neural structures.
METHODS: Ten fresh cadavers were studied. Screws, 5.0 and 6.0 mm, were inserted from T1 to T6 and T7 to T12, respectively. Pedicle perforations and fractures were recorded upon screw insertion and final positioning (nonthreaded portion facing medially) after a wide laminectomy. Pullout strength of novel and conventional screws were then tested using an Instron machine in an artificial bone substitute.
RESULTS: A total of 240 thoracic pedicle screws were inserted. Of them, 88.8% (213 screws) were fully contained during screw insertion. There were 5.0% (12 screws) grade 1 medial perforations and 6.2% (15 screws) grade 1 lateral perforations during screw insertion. Upon final positioning, 93.8% (225 screws) were fully contained. All grade 1 medial perforations, which occurred during insertion, were converted to grade 0. No dural or nerve root injuries occurred. Pedicle split fractures were noted in 6.7% (16 screws). The use of medial, partial nonthreaded screws reduced the overall perforation rate from 11.2% to 6.2%. The mean pullout load for the 5 mm fully threaded screw versus medial, partial nonthreaded was 1419.3±106.1 N (1275.8-1538.8 N) and 1336.6±44.2 N (1293.0-1405.1 N) respectively, whereas 6 mm pullout load averaged 2126.0±134.8 N (1986.3-2338.3 N) and 2036.5±210.0 N (1818.4-2355.9 N). The difference was not statistically significant.
CONCLUSIONS: The use of medial, partial nonthreaded pedicle screws reduced the medial perforation rate from 5.0% to 0%; however, the pullout strength was not significantly reduced. The use of this novel screw can potentially reduce the incidence of nerve injury or irritation after medial pedicle perforations.

Matched MeSH terms: Cadaver
In vitro comparison of linear vs triangular screw configuration to stabilize complete uniarticular parasagittal fractures of the proximal phalanx in horses

Labens R, Khairuddin NH, Murray M, Jermyn K, Ahmad RS

Vet Surg, 2019 Jan;48(1):96-104.
PMID: 30403407 DOI: 10.1111/vsu.13123

OBJECTIVE: To assess fracture gap reduction and stability of linear vs triangular 4.5-mm lag screw repair of experimental, uniarticular, and complete forelimb proximal phalanx (P1) fractures.
STUDY DESIGN: Experimental.
SAMPLE POPULATION: Fourteen equine cadaver limbs/horses.
METHODS: Simulated fractures were repaired with 2 lag screws under 4-Nm insertion torque (linear repair). Computed tomography (CT) imaging was performed with the leg unloaded and loaded to forces generated while walking. The fracture repair was revised to include 3 lag screws placed with the same insertion torque (triangular repair) prior to CT. The width of the fracture gap was assessed qualitatively by 2 observers and graded on the basis of gap measurements relative to the average voxel size at dorsal, mid, and palmar P1 sites. Interobserver agreement was assessed with Cohen's κ. The effect of repair type, loading condition, and measurement site on fracture gap grades was evaluated by using Kendall's τ-b correlation coefficients and paired nonparametric tests. Significance was set at P ≤ .05.
RESULTS: Agreement between loading and fracture gap widening was fair in triangular (κ = 0.53) and excellent in linear (κ = 0.81) repairs. Loading resulted in fracture gap distraction in linear repairs (Plinear = .008). Triangular repairs reduced fractures better irrespective of loading (Punloaded = .003; Ploaded

Matched MeSH terms: Cadaver
Recovery of forensically important insect larvae from human cadavers in Malaysia (1993-1996)

Lee HL

Malays J Pathol, 1996 Dec;18(2):125-7.
PMID: 10879234

Forensically important entomological specimens recovered from 95 forensic cases of human cadavers from April 1993 to May 1996 in Malaysia were identified and analysed. The results indicated that 73.7% of these specimens were Chrysomya species, occurring either as single or mixed infestations. Of these, the most prominent species were Ch megacephala (F.) and Ch rufifacies (Macquart). Other fly maggots recovered included Sarcophaga spp., Lucilia spp. and Hermetia spp., mostly occurring together with other calliphorine flies. A member of Muscidae fly, Ophyra spp. was also recovered for the first time.

Matched MeSH terms: Cadaver*
Recovery of forensically important entomological specimens from human cadavers in Malaysia--an update

Lee HL

Malays J Pathol, 1989 Aug;11:33-6.
PMID: 2632998

A total of 101 entomological specimens recovered from human cadavers were processed and studied. Analysis of the data indicated that about 95% of these specimens were maggots of flies. Maggots of the blowfly Chrysomya (Family: Calliphoridae) especially Ch. rufifacis and Ch. megacephala were predominantly found in 77 cases (76.2%) while larvae of several other flies of the genera Sarcophaga, Calliphora, Lucilia and hermetia were also recovered. It was notable that Musca domestica or other related flies were not found in all these specimens. The age of these larvae was useful in the determination of the minimum time lapsed after death. However, more biological studies on animal carcases should be conducted for more accurate determinations. Methods of collection, preservation and despatching of specimens were also discussed.

Matched MeSH terms: Cadaver*
Fulltext Review of forensically important entomological specimens in the period of 1972 - 2002

Lee HL, Krishnasamy M, Abdullah AG, Jeffery J

Trop Biomed, 2004 Dec;21(2):69-75.
PMID: 16493401

Forensic entomological specimens received by the Unit of Medical Entomology, IMR., from hospitals and the police in Malaysia in the last 3 decades (1972 - 2002) are reviewed. A total of 448 specimens were received. From these, 538 identifications were made with the following results: Eighteen species of cyclorrphaga flies were identified consisting of Chrysomya megacephala (Fabricius) 215 cases (47.99%), Ch. rufifacies (Masquart) 132 (29.46%), Ch. villeneuvi Patton 10 (2.23%), Ch. nigripes Aubertin 7 (1.56%), Ch. bezziana Villeneuve 4 (0.89%), Ch. pinguis (Walker) 1 (0.22%), Chrysomya sp. 47 (10.49%), Sarcophaga sp. 28 (6.25%), Lucilia sp. 21 (4.69%), Hermetia sp. 15 (3.35%), He. illucens (Linnaeus) 1 (0.22%), Hemipyrellia ligurriens (Wiedemann) 3 (0.67%), Hemipyrellia sp. 2 (0.45%), Ophyra spinigera 1 (0.22%), Ophyra sp. 6 (1.34%), Calliphora sp. 24 (5.36%), Synthesiomyia nudiseta (Wulp) 1 (0.22%) and Eristalis sp. 1 (0.22%). Other non - fly insect specimens are Pthirus pubis (Linnaeus) (Pubic louse) 2 (0.45%) and Coleoptera (Beetles) 1 (0.22%). Ch. megacephala and Ch. rufifacies were the commonest species found in cadavers from different ecological habitats. Sy. nudiseta is an uncommon species, thus far found only on cadavers from indoors. Sy. nudiseta is reported for the second time in Peninsular Malaysia. A total of 329 cases (73.44%) had a single fly infestation, 109 cases (24.33%) had double fly infestation and 10 cases (2.23%) had triple fly infestation. Five cases (1.12%) had eggs and 3 cases (0.67%) had larval stages that were not identifiable. No arthropods were retrieved from cadavers in 8 cases (1.79%). In conclusion, although large number of fly species were found on human cadavers, the predominant species are still those of Chrysomya.

Matched MeSH terms: Cadaver
Fulltext Awareness, attitude and willingness regards cadaveric organ donation and awaness of brain death among Sarawakians in Kuching, Malaysia

Lim KG, Tan AS, Wong SC, Tee EG, Burud IA

Ceylon Med J, 2022 Nov 30;67(3):113-120.
PMID: 37933994 DOI: 10.4038/cmj.v67i3.9699
Matched MeSH terms: Cadaver
Fulltext A new clinical test for radial tunnel syndrome--the Rule-of-Nine test: a cadaveric study

Loh YC, Lam WL, Stanley JK, Soames RW

J Orthop Surg (Hong Kong), 2004 Jun;12(1):83-6.
PMID: 15237127 DOI: 10.1177/230949900401200115

Radial tunnel syndrome refers to pain on the lateral aspect of the forearm as a result of compression of the posterior interosseous nerve within a tunnel with specific anatomical boundaries. Diagnosis of the condition is difficult because of its close association with lateral epicondylitis, which warrants different methods of treatment. Based on a cadaveric study, a new clinical test, the Rule-of-Nine test, is proposed to improve the diagnostic accuracy in radial tunnel syndrome. The test involves constructing 9 equal squares on the anterior aspect of the forearm and noting those squares where tenderness can be elicited.

Matched MeSH terms: Cadaver
Role of simulation in arthroscopy training

Madan SS, Pai DR

Simul Healthc, 2014 Apr;9(2):127-35.
PMID: 24096921 DOI: 10.1097/SIH.0b013e3182a86165

Arthroscopy uses a completely different skill set compared with open orthopedic surgery. Hitherto, arthroscopy had not been given enough emphasis in the core orthopedic curricula. Simulation has been seen as an excellent way to teach the skills required in arthroscopy. The simulators used for arthroscopy training can be broadly classified into physical simulators such as cadavers, animals, models and box trainers, virtual-reality simulators, and hybrid simulators that combine virtual-reality simulation with physical components that allow real tactile feedback. The advantages and disadvantages of each of these types have been described in this article. The factors that determine skill acquisition using these simulators have been highlighted. In conclusion, simulation seems to be a valuable tool for arthroscopy training, although further studies are needed to state whether this translates into better operative skill on real patients.

Matched MeSH terms: Cadaver
Patterns of oviposition and development of Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) and Chrysomya rufifacies (Macquart) (Diptera: Calliphoridae) on burned rabbit carcasses

Mahat NA, Zainol-Abidin NL, Nordin NH, Abdul-Wahab R, Jayaprakash PT

Forensic Sci Int, 2016 Mar;260:9-13.
PMID: 26779962 DOI: 10.1016/j.forsciint.2015.12.047

Considering that crimes against animals such as illegal killing and cruelty have been alarmingly increasing and since burning is one of the common ways for disposing cadavers, ability to estimate minimum postmortem interval (PMI) using entomological data merits consideration. Chrysomya megacephala and Chrysomya rufifacies are common necrophagous species recovered from cadavers in many countries including Malaysia. Specific studies focusing on the oviposition and developmental patterns of both species on cadavers manifesting different levels of burn as described by the Crow-Glassman Scale (CGS) remain scarce. In four replicates, rabbit carcasses were burned to CGS levels #1, #2 and #3 by varying the amount of petrol used and duration of burning. Oviposition by C. megacephala and C. rufifacies was delayed by one day in the case of carcasses burned to the CGS level #3 (p<0.05) when compared with that of controls. Such delay in oviposition was not observed in the CGS level #1 and #2 carcasses. No significant differences (p>0.05) in the duration of development were found between control and burned carcasses. These findings deserve consideration while estimating minimum PMI since burning as a mean for disposing animal and human cadavers is gaining popularity.

Matched MeSH terms: Cadaver
Fulltext Malathion extraction from larvae of Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) for determining death due to malathion

Mahat NA, Jayaprakash PT, Zafarina Z

Trop Biomed, 2012 Mar;29(1):9-17.
PMID: 22543598 MyJurnal

The use of Chrysomya megacephala larvae for detecting malathion for diagnosing the cause of death was investigated. This could prove useful when the visceral organs have become liquefied during decomposition and therefore cannot be sampled. A field experiment was conducted in which C. megacephala were allowed to colonise naturally the corpses of rabbits that had died of malathion poisoning. The concentration of malathion increased gradually during the larval stages of C. megacephala reaching the maximum concentration in the third instar larvae. The concentration of malathion declined during prepupal stage and reached its lowest level among tenerals. The average malathion concentrations in C. megacephala growing in poisoned rabbit corpses left in a sunlit habitat were significantly higher (p<0.05) than those growing on poisoned rabbits left in a shaded habitat. The concentrations of malathion in the different stages of development of C. megacephala were moderately correlated (r = 0.51-0.69) with the administered doses as well as with those estimated in visceral organs. Thus, it would not be reliable to suggest the formulation of mathematical algorithms for relating the concentration of malathion found in the different stages of development of C. megacephala with those found in the visceral organs. However, in the context of forensic investigation, the qualitative detection of malathion in C. megacephala may prove useful in diagnosing the cause of death, since malathion is a common cause of accidental and suicidal deaths.

Matched MeSH terms: Cadaver
Fulltext Cadaver dissection: attitude and perception of its importance among medical and health science students in learning anatomy

Manah Chandra Changmai, Kastury Gohain, Akma Asyira Binti Zulkarnaie

Malaysian Journal of Medicine and Health Sciences, 2020;16(107):52-56.
MyJurnal

Introduction: Anatomy continues to be an important basic subject in medicine and other related health sciences which is delivered by method of either through lectures or demonstration. Thus, having a gross anatomy practi- cal with cadaver is a crucial phase for the students to have a deep understanding about anatomy of human body. The objective of this study is to explore the attitude and perception of medical and health science students in the importance of cadaver dissection in learning anatomy. Methods: A cross-sectional study was conducted by distrib- uting closed structured questionnaires to 270 respondents consisting of socio-demographic in part one, frequency on practical with dissected cadaver in part two, the attitude of student towards the dissected cadaver in part three, suggestion for improvement during practical with cadaver in part four and the value of cadavers during practical in part five. The collected data were processed by using Statistical Package for Social Sciences (SPSS) version 23.0. Result: Descriptive statistical evaluation shows majority of the students perform practical with cadavers. They find cadaveric dissection exciting with no feeling of stress and anxiety. Many of them never had an emotional shock when exposing themselves to the cadavers for the first time accepting the cadaveric dissection ethically. The students recommended keeping cadaveric dissection in the health science curriculum. Conclusion: The cadaveric dissection helps in grounded understanding of anatomy. It promotes and develops psychomotor skills in students. The findings of the study discovered dissection to be a motivating tool in learning anatomy.

Matched MeSH terms: Cadaver
Fulltext Novel method to estimate the length of the palmaris longus tendon

Manohar, A., Ramal, L.

Malaysian Journal of Medicine and Health Sciences, 2013;9(2):19-24.
MyJurnal

Palmaris longus is tendon of choice in reconstructive, plastic and cosmetic surgeries. Thus, a suitable length is required and it would be more convenient if the length of the tendon of the palmaris longus could be estimated before harvesting. This study was carried out to determine the relationship between the length and width of the palmaris longus tendon and the length of forearm and hand, the relationship between the length and the width of the palmaris longus tendon and wrist width and wrist circumference, as well as to identify a technique to estimate the length of the tendinous part of palmaris longus before harvesting it. A standardized proforma was used to collect the data of each of the 31 cadavers upper limbs. These data were analysed using SPSS software version 17. It is important to note that p value less than 0.05 is considered as statistically significant in this study. Out of the 31 upper limbs, palmaris longus tendon was absent in 3 (9.68%). Meanwhile, the mean length and width of the palmaris longus tendon was found to be 16.20 cm and 0.48 cm, respectively. The mean length of the forearm and hand was 26.6 cm and 21.2 cm, respectively. The mean width of the wrist and wrist circumference was 8.2 cm and 14.82 cm, respectively. These indicate a significant and moderate relationship between the length of palmaris longus tendon and the length of forearm (r = 0.49, r2 = 0.24, p < 0.01). In addition, there was also a significant relationship between the length of palmaris longus tendon and the length of hand (r = 0.56, p < 0.01). This paper presents the technique used to estimate the length and width of the tendinous part of palmaris longus before harvesting. The length of palmaris longus can be estimated pre-operatively by measuring the length of the hand by using the technique explained in this paper.

Matched MeSH terms: Cadaver
Some anthropometric studies of the femur of the male West Malaysian Chinese

Manuel JK, Yusof Bin Mohd M

Am. J. Phys. Anthropol., 1974 Jul;41(1):133-7.
PMID: 4843090
Matched MeSH terms: Cadaver
Species richness of scavenger insects on different carcass types

Mashaly AM, Al-Ajmi RA, Rady A, Al-Musawi Z, Farrukh A

Trop Biomed, 2019 Sep 01;36(3):630-639.
PMID: 33597485

The type and amount of resources available significantly influences the structure and dynamics of food webs. In this study, we analyzed differences in species richness of scavengers based on carcass type in Riyadh, Saudi Arabia. We collected insects from experimental carcasses of three different types, domestic dogs (Canidae, Canis lupus familiaris), Hijazi goats (Bovidae, Capra aegagrus hircus), and camels (Camelidae, Camelus dromedarius). Data collection was conducted during the decay stage in June, 2016. We used mitochondrial cytochrome c oxidase subunit I (mtCOI) barcodes as a marker for the molecular identification of the scavenger insects. The results showed that there were more insects on the camels and goats than the dogs. In total, seven species were found on all carrions. Six species were found on the camels and goats, but only five were found on the dog. Musca domestica was the most collected species of flies whereas, Necrobia rufipes was the most collected species of beetles. Overall, this study showed that carrion type had an effect on the type and number of insects attracted to the carrions. Thus, one of the significant factors that influence the associated scavenger assemblage is a carcass type.

Matched MeSH terms: Cadaver*
Anatomical variations of median nerve formation, distribution and possible communication with other nerves in preserved human cadavers

Mat Taib CN, Hassan SN, Esa N, Mohd Moklas MA, San AA

Folia Morphol (Warsz), 2016 09 26;76(1):38-43.
PMID: 27665953 DOI: 10.5603/FM.a2016.0045

Formation, distribution and possible communication of the median nerve are essential to know in treatment and surgeries of various conditions of injuries e.g. repair or reconstruction of the median nerve post traumatic accident. In the present study, 44 upper limbs were dissected. Root forming the median nerve, the median nerve in relation with the axillary artery and communication of the median nerve with other nerves were noted.

Matched MeSH terms: Cadaver

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