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Fulltext Anaesthetists' nightmare: masseter spasm after induction in an undiagnosed case of myotonia congenita

Looi I, Bakar AAA, Lim CH, Khoo TH, Samuel PE

Med J Malaysia, 2008 Dec;63(5):423-5.
PMID: 19803309

We report an undiagnosed case of myotonia congenita in a 24-year-old previously healthy primigravida, who developed life threatening masseter spasm following a standard dose of intravenous suxamethonium for induction of anaesthesia. Neither the patient nor the anaesthetist was aware of the diagnosis before this potentially lethal complication occurred.
Are deep eutectic solvents benign or toxic?

Hayyan M, Hashim MA, Hayyan A, Al-Saadi MA, AlNashef IM, Mirghani ME, et al.

Chemosphere, 2013 Feb;90(7):2193-5.
PMID: 23200570 DOI: 10.1016/j.chemosphere.2012.11.004

In continuation of investigation for environmentally benign protocol for new solvents termed deep eutectic solvents (DESs), it is herein reported results concerning the toxicity and cytotoxicity of choline chloride (ChCl) based DESs with four hydrogen bond donors including glycerine, ethylene glycol, triethylene glycol and urea. The toxicity was investigated using two Gram positive bacteria Bacillus subtilis and Staphylococcus aureus, and two Gram negative bacteria Escherichia coli and Pseudomonas aeruginosa. The cytotoxicity effect was tested using the Artemia salina leach. It was found that there was no toxic effect for the tested DESs on all of the studied bacteria confirming their benign effects on these bacteria. Nevertheless, it was found that the cytotoxicity of DESs was much higher than their individual components (e.g. glycerine, ChCl) indicating that their toxicological behavior is different. For our best knowledge this is the first time that toxicity and cytotoxicity of DESs were studied. The toxicity and cytotoxicity of DESs varied depending on the structure of components. Careful usage of the terms non-toxicity and biodegradability must be considered. More investigation on this matter is required.
STR data for the 13 CODIS loci in Singapore Malays

Ang HC, Sornarajah R, Lim SE, Syn CK, Tan-Siew WF, Chow ST, et al.

Forensic Sci Int, 2005 Mar 10;148(2-3):243-5.
PMID: 15639622

Allele frequencies for the 13 CODIS (Combined DNA Index System, USA) STR loci included in the AmpFISTR Profiler Plus and AmpFISTR Cofiler kits (Applied Biosystems, Foster City, USA) were determined in a sample of 197 unrelated Malays in Singapore.
Microstructure and properties of crystalline bioglass compositions prepared by polymeric route

Doreya MI, Mona EW, Afaf ES, Hanan HB

Med J Malaysia, 2004 May;59 Suppl B:21-2.
PMID: 15468799

The standard bioglass composition GS45 as well as with excess silica GS50 or with the addition of 5% titanium oxide GS45+Ti5, were prepared by the polymeric route. The different glass components were added to the formed polymer. Firing at 700 degrees C gave an amorphous product with microporous texture that readily crystallizes out at 900 degrees C. The prepared materials were highly porous with two modes of pore system micro-pores and macro-pores with a size ranging between 100 microm to 0.006 microm and a porosity reaching 73%. The measured bulk density was between 0.36 to 1.1g/cm3. The fired material preserved the former structure of the polymer precursor. Biocompatibility was verified in vitro and vivo. IR of the specimens previously immersed in SBF revealed the formation of apatite like layer. While the histology sections of implants in rate femurs showed new bone tissue or bone trabeculae after 21 days.
Effect of induced expression of an antimicrobial peptide melittin on Chlamydia trachomatis and Mycoplasma hominis infections in vivo

Lazarev VN, Shkarupeta MM, Titova GA, Kostrjukova ES, Akopian TA, Govorun VM

Biochem Biophys Res Commun, 2005 Dec 16;338(2):946-50.
PMID: 16246304

A plasmid construct was designed in which the gene of antimicrobial peptide melittin is controlled by the tetracycline-responsive promoter of human cytomegalovirus, aided by a constitutively expressed trans-activator protein gene. Its vaginal administration and induction of melittin gene transcription with doxycycline markedly suppressed subsequent genital tract infection of mice by Mycoplasma hominis and Chlamydia trachomatis. At least half of the melittin-protected animals proved free of either pathogen within 3-4 weeks. Recombinant plasmids expressing genes of antimicrobial peptides hold much promise as agents for prevention and control of urogenital latent infections.
Bathroom greywater recycling using polyelectrolyte-complex bilayer membrane: Advanced study of membrane structure and treatment efficiency

Oh KS, Poh PE, Chong MN, Chan ES, Lau EV, Saint CP

Carbohydr Polym, 2016 Sep 05;148:161-70.
PMID: 27185127 DOI: 10.1016/j.carbpol.2016.04.039

Polyelectrolyte-complex bilayer membrane (PCBM) was fabricated using biodegradable chitosan and alginate polymers for subsequent application in the treatment of bathroom greywater. In this study, the properties of PCBMs were studied and it was found that the formation of polyelectrolyte network reduced the molecular weight cut-off (MWCO) from 242kDa in chitosan membrane to 2.71kDa in PCBM. The decrease in MWCO of PCBM results in better greywater treatment efficiency, subsequently demonstrated in a greywater filtration study where treated greywater effluent met the household reclaimed water standard of <2 NTU turbidity and <30ppm total suspended solids (TSS). In addition, a further 20% improvement in chemical oxygen demand (COD) removal was achieved as compared to a single layer chitosan membrane. Results from this study show that the biodegradable PCBM is a potential membrane material in producing clean treated greywater for non-potable applications.
Mental health in Asia: social improvements and challenges

Tseng WS, Ebata K, Kim KI, Krahl W, Kua EH, Lu Q, et al.

Int J Soc Psychiatry, 2001;47(1):8-23.
PMID: 11322408

Remarkable improvements in economic conditions and a considerable upgrade in the quality of life have been observed in many parts of Asia during the past several decades. At the same time, many mental health challenges face the people of Asia. Various social mental health indexes are reviewed here, with available data from China, Japan, Korea, Singapore, Malaysia, and other Asian societies. The data are compared with data from the United States, Australia in the Pacific Rim, and some other Western countries to examine patterns of similarity or difference between East and West in the process of modernization. Common trends in mental health issues associated with rapid sociocultural change observed in different Asian societies are discussed, as well as the relative shortage of mental health personnel available in many Asian societies. It is emphasized that, in addition to expanding psychiatric services, there is an even more urgent need to promote mental health knowledge and concern through education in the general population. Mental health needs to be cultivated and maintained by social forces and cultural strengths. It is stressed that there is a challenge for Asian people to advance mental health beyond economic development in the 21st century.
Fulltext Prevalence of primary aldosteronism among Asian hypertensive patients in Singapore

Loh KC, Koay ES, Khaw MC, Emmanuel SC, Young WF

J Clin Endocrinol Metab, 2000 Aug;85(8):2854-9.
PMID: 10946893

Recent studies using the ratio of plasma aldosterone concentration (PAC) to PRA as the screening test for primary aldosteronism in hypertensive populations suggested that the prevalence may be as high as 5-15%, with well over half of the subjects having normal serum potassium concentrations. Despite an increasing clinical awareness of this entity, many clinicians are reluctant to consider routine screening for primary aldosteronism in essential hypertensive patients because there are few community-based prevalence studies of primary aldosteronism in different populations. Furthermore, genetic and environmental differences may affect the prevalence and presentation of primary aldosteronism in distinct populations. This study was designed to determine the prevalence of primary aldosteronism in the predominantly Chinese population in Singapore. Three hundred and fifty unselected adult hypertensive patients attending two primary care clinics had random ambulatory measurements for PAC (nanograms per dL) and PRA (nanograms per mL/h). Serum urea, creatinine, and electrolyte measurements were obtained simultaneously. Subjects with renal insufficiency (serum creatinine, >140 micromol/L) and those treated with glucocorticoids or spironolactone were excluded. Screening was considered positive if the PAC: PRA ratio was more than 20 and the PAC was more than 15 ng/dL (>416 pmol/L). Primary aldosteronism was confirmed with the determination of PAC after 2 L saline administered iv over 4 h. Adrenal computed tomographic (CT) scans were performed in biochemically confirmed cases of primary aldosteronism. Further localization with adrenal vein sampling was carried out in selected patients with equivocal findings on adrenal CT scan. Sixty-three (18%) of the 350 hypertensive patients (215 women and 135 men; age range, 23-75 yr) were screened positive for primary aldosteronism. Only 13 of these 63 subjects (21%) were hypokalemic (serum potassium, <3.5 mmol/L). Confirmatory studies were carried out in 56 (89%) of the subjects with a positive PAC:PRA ratio. Using a PAC above 10 ng/dL (>277 pmol/L) after saline infusion as the diagnostic cut-off, 16 of the 56 patients had biochemically confirmed primary aldosteronism. Hypokalemia was found in 6 of the 16 patients (37.5%) with primary aldosteronism. Subtype evaluation with adrenal CT scan and adrenal vein sampling indicated that half of the patients with primary aldosteronism may have had potentially curable unilateral adrenal adenoma. Our data suggest that primary aldosteronism occurs in at least 5% of the adult Asian hypertensive population, and approximately half of these individuals may have potentially curable, unilateral, aldosterone-producing adrenal adenoma. Our findings also confirm the poor predictive value of hypokalemia in both the diagnosis and the exclusion of primary aldosteronism.
Medicinal plants: conception / contraception

Chaing HS, Merino-chavez G, Yang LL, Wang FN, Hafez ES

Adv Contracept Deliv Syst, 1994;10(3-4):355-63.
PMID: 12287843
Fulltext A study on the food and nutritional status of the Armed Forces

Chong YH, Tee ES, Ng TKW, Yap SB, Mok SK

Med J Malaysia, 1982 Mar;37(1):46-51.
PMID: 7121346

The nutritional status of 158 soldiers drawn from four army camps in Selangor, Negeri Sembilan and Pahang has been assessed by biochemical procedures, nutritional anthropometry and dietary (chemical) analysis. Biochemical assessment showed that with the exception of thiamin and riboflavin nutriture, the nutritional status of the soldiers appeared generally satisfactory and seemed to have improved slightly over those examined by the Inter-Departmental Committee on Nutrition and National Defence (ICNND), USA, in 1962 by similar methods. The anthropometric assessment indicated that the present day soldiers have the same mean height as those examined in 1962. Although they appeared slightly heavier, obesity did not seem to be a problem. Chemical analysis of their diet showed that there is room for improvement in the dietary supply of vitamin A and some of the water-soluble vitamins, particularly riboflavin, thiamin and vitamin C. There was also a significant discrepancy between the nutritional content of the chemically-analysed cooked meals and those calculated from the ration scales using Food Composition Tables, suggesting a need to review the current system of food supply,
preparation and food sertnce unthin army camps in
Malaysia.
Fulltext Development of Photovoltaic Module with Fabricated and Evaluated Novel Backsheet-Based Biocomposite Materials

Alaaeddin MH, Sapuan SM, Zuhri MYM, Zainudin ES, M Al-Oqla F

Materials (Basel), 2019 Sep 17;12(18).
PMID: 31533207 DOI: 10.3390/ma12183007

Photovoltaic backsheets have considerable impact on the collective performance of solar cells. Material components should withstand certain temperatures and loads while maintaining high thermal stability under various weather conditions. Solar modules must demonstrate increased reliability, adequate performance, safety, and durability throughout the course of their lifetime. This work presents a novel solar module. The module consists of an innovative polyvinylidene fluoride-short sugar palm fiber (PVDF-SSPF) composite backsheet within its structure. It was electrically and thermally evaluated. The current-voltage characteristics (I-V) were obtained using the solar module analyzer, PROVA 210PV. A thermal evaluation was accomplished using a temperature device, SDL200. The thermal test consisted of two different assessments. The first targeted the surface and backsheet of the developed module to correlate their performance from within. The second assessment compared the thermal performance of the fabricated backsheet with the conventional one. Both tests were combined into a heatmap analysis to further understand the thermal performance. Results revealed that the developed module exhibited reasonable electrical efficiency, achieving appropriate and balanced I-V curves. PVDF-SSPF backsheets proved to be thermally stable by displaying less heat absorbance and better temperature shifts. Additional research efforts are highly encouraged to investigate other characteristics. To enhance performance, further analyses are needed such as the damp heat analysis, accelerated aging analysis, and heat dissipation phenomena.
Fulltext Denaturing gradient-gel electrophoresis screening of familial defective apolipoprotein B-100 in a mixed Asian cohort: two cases of arginine3500-->tryptophan mutation associated with a unique haplotype

Choong ML, Koay ES, Khoo KL, Khaw MC, Sethi SK

Clin Chem, 1997 Jun;43(6 Pt 1):916-23.
PMID: 9191540

The Arg-to-Trp substitution at codon 3500 in the apolipoprotein (apo) B-100 gene is established as a cause of familial defective apo B-100 (FDB), a functional mutation, resulting in reduced LDL receptor binding and manifest hypercholesterolemia. In a search for similar mutations in 163 Malaysians, we screened the putative receptor-binding region (codons 3456-3553) of the apo B-100 gene by PCR amplification and denaturing gradient-gel electrophoresis. Four single-base mutations were detected and confirmed by DNA sequencing. Two females, a Chinese and a Malay, had the same CGG3500-->TGG mutation, resulting in an Arg3500-to-Trp substitution. This is the second published report of such an independent mutation involving the same codon as the established Arg3500-to-Gln mutation. The two other mutations detected, CTT3517-->CTG and GCC3527-->GCT, resulted in degenerate codons with no amino acid substitutions. All four mutations were associated with a unique apo B haplotype, different from those found in Caucasian FDB patients but concurring with that previously reported for two other Asians with FDB.
Fulltext Investigation of natural environmental radioactivity concentration in soil of coastaline area of Ado-Odo/Ota Nigeria and its radiological implications

Joel ES, Maxwell O, Adewoyin OO, Olawole OC, Arijaje TE, Embong Z, et al.

Sci Rep, 2019 03 12;9(1):4219.
PMID: 30862825 DOI: 10.1038/s41598-019-40884-0

Natural radioactivity in coastaline area soil of Ado-Odo/Ota has been carried out to ascertain the presence of radionuclides using gamma-ray spectroscopy (HPGe detector). The result showed that U-238, Th-232 and K-40 ranged from 24 ± 7-49 ± 10; 67 ± 6-120 ± 9 and 88 ± 17-139 ± 20 Bqkg-1 respectively. The radium equivalent for the samples ranged from 132.51 to 230.91 Bqkg-1 with mean value of 185.89 Bqkg-1. The mean value for the gamma dose rate for the soil samples was estimated to be 81.32 nGyh-1. The estimated values of annual effective dose equivalent ranged from 0.61 to 1.07 mSv y-1. The estimation of alpha index representative (Iα) ranged from 0.12 to 0.24 with mean value of 0.21 while the gamma representative index ranged between 0.465 and 0.810. The activity utilization index of the soil samples ranged from 1.09 to 1.89 with mean value of 1.53. The radiological implication in the study area has shown that the soil samples with gamma dose rate value of 89.99 nGyh-1, 94.39 nGyh-1, 97.40 nGyh-1 and 101.04 nGyh-1 respectively are higher than the recommended value of 80 nGyh-1 and may pose health implication for long term exposure.
Fulltext Corn Starch (Zea mays) Biopolymer Plastic Reaction in Combination with Sorbitol and Glycerol

Hazrol MD, Sapuan SM, Zainudin ES, Zuhri MYM, Abdul Wahab NI

Polymers (Basel), 2021 Jan 12;13(2).
PMID: 33445740 DOI: 10.3390/polym13020242

The research included corn starch (CS) films using sorbitol (S), glycerol (G), and their combination (SG) as plasticizers at 30, 45, and 60 wt %, with a traditional solution casting technique. The introduction of plasticizer to CS film-forming solutions led to solving the fragility and brittleness of CS films. The increased concentration of plasticizers contributed to an improvement in film thickness, weight, and humidity. Conversely, plasticized films reduced their density and water absorption, with increasing plasticizer concentrations. The increase in the amount of the plasticizer from 30 to 60% showed a lower impact on the moisture content and water absorption of S-plasticized films. The S30-plasticized films also showed outstanding mechanical properties with 13.62 MPa and 495.97 MPa, for tensile stress and tensile modulus, respectively. Glycerol and-sorbitol/glycerol plasticizer (G and SG) films showed higher moisture content and water absorption relative to S-plasticized films. This study has shown that the amount and type of plasticizers significantly affect the appearances, physical, morphological, and mechanical properties of the corn starch biopolymer plastic.
Fulltext Nutritional status, dietary intake patterns and nutrition knowledge of children aged 5-6 years attending kindergartens in the Klang Valley, Malaysia

Poh BK, Kathryn Tham BL, Wong SN, Winnie Chee SS, Tee ES

Malays J Nutr, 2012 Aug;18(2):231-42.
PMID: 24575669 MyJurnal

Early childhood is a period during which many factors influence the development of lifelong eating habits. This study aimed to assess the nutritional status of young children and to determine factors related to eating habits.
Extended half-life pegylated, full-length recombinant factor VIII for prophylaxis in children with severe haemophilia A

Mullins ES, Stasyshyn O, Alvarez-Román MT, Osman D, Liesner R, Engl W, et al.

Haemophilia, 2017 Mar;23(2):238-246.
PMID: 27891721 DOI: 10.1111/hae.13119

INTRODUCTION: Primary factor VIII (FVIII) prophylaxis is the optimal treatment in children with severe haemophilia A. They are expected to benefit from extended half-life (T1/2 ) FVIII coverage by reduced infusion frequency while maintaining haemostatic efficacy.
AIMS: To determine immunogenicity, pharmacokinetics (PK), efficacy, safety and quality of life of prophylaxis with a polyethylene glycol (peg)-ylated FVIII (BAX 855) based on full-length recombinant FVIII (ADVATE) in paediatric previously treated patients (PTPs) with severe haemophilia A.
METHODS: PTPs <12 years without history of FVIII inhibitors received twice-weekly infusions of 50 ± 10 IU kg(-1) BAX 855 for ≥50 exposure days. Prophylactic dose increases to ≤80 IU kg(-1) were allowed under predefined conditions. PK was evaluated after single infusions of 60 ± 5 IU kg(-1) .
RESULTS: T1/2 and mean residence time were extended 1.3- to 1.5-fold compared to ADVATE (n = 31), depending on the analysis used. The point estimate for the mean annualized bleeding rate in 66 subjects receiving a median of 1.9 weekly infusions of 51.3 IU kg(-1) of BAX 855 each was 3.04 (median 2.0); 1.10 (median 0) for joint and 1.16 (median 0) for spontaneous bleeds. Overall, 38% of subjects had zero bleeds. No bleeds were severe. Haemostatic efficacy was rated excellent or good for 90% of bleeds; 91% were treated with one or two infusions. In 8/14 subjects all target joints resolved. No subject developed FVIII inhibitors or persistent binding antibodies that affected safety or efficacy. No adverse reactions occurred.
CONCLUSION: Twice-weekly prophylaxis with BAX 855 was safe and efficacious in paediatric PTPs with severe haemophilia A.
Aerosolised Mesenchymal Stem Cells Expressing Angiopoietin-1 Enhances Airway Repair

Halim NSS, Ch'ng ES, Kardia E, Ali SA, Radzi R, Yahaya BH

Stem Cell Rev Rep, 2019 02;15(1):112-125.
PMID: 30178289 DOI: 10.1007/s12015-018-9844-7

BACKGROUND: The aim of this study was to investigate the effects of MSCs and MSC-expressing ANGPT1 (MSC-pANGPT1) treatment via aerosolisation in alleviating the asthma-related airway inflammation in the rabbit model.
METHODS: Rabbits were sensitised and challenged with both intraperitoneal injection and inhalation of ovalbumin (Ova). MSCs and MSC-pANGPT1 cells were aerosolised into rabbit lungs using the MicroSprayer® Aerosolizer Model IA-1B 48 h after injury. The post mortem was performed 3 days following cell delivery. Histopathological assessments of the lung tissues and inflammatory response were quantitatively scored following treatments.
RESULT(S): Administration of aerosolised MSCs and MSC-pANGPT1 were significantly reduced inflammation of the airways (p S): Our findings suggest that treatment with MSCs alone attenuated airway inflammation and structural changes of the airway. Treatment with MSC-pANGPT1 provided an additional effect in reducing the expression levels of various pro-inflammatory genes. Both of these treatment enhancing airway repair and therefore may provide a basis for the development of an innovative approach for the treatment and prevention of airway inflammatory diseases.
Fulltext Radionuclides proportion and radiological risk assessment of soil samples collected in Covenant University, Ota, Ogun State Nigeria

Adewoyin OO, Omeje M, Joel ES, Akinwumi SA, Ehi-Eromoseled CO, Embong Z

MethodsX, 2018;5:1419-1426.
PMID: 30456176 DOI: 10.1016/j.mex.2018.10.023

The activity levels of 238U, 232Th and 40K in soil surrounding major office complexes in Covenant University were analyzed for radiological hazards to determine the safety of the residents in such environment. Sixteen (16) soil samples were collected, prepared and sent to Acme laboratory in Canada for analysis with the use of high purity germanium detector. The mean activity concentrations of 238U, 232Th and 40K were found to be 45 ± 10, 135 ± 8 and 195 ± 20 respectively. The concentrations of 238U and 232Th were found to be higher than the world recommended standard of 35 and 30, while the result for 40K was noted to be lower than the world safe limit. The average values of Raeq, D, AED, Iyr, Hex and ELCR in this study were estimated to be 252.33 Bq/kg, 110.15 nGy/h, 0.13 mSv/y, 1.78, 0.68 and 0.47 × 10-3 respectively. It was noticed that none of the measured parameters was higher than the internationally recommended safe limits. 232Th was found to be the major contributor to the environmental radionuclides in the area of study. Therefore, the inhabitants of the office complexes whose environment was assessed are considered not be exposed to any radiological hazards.
Fulltext Microbiology of diabetic foot infections in three district hospital in Malaysia and comparison with South East Asian Countries

Kow RY, Low CL, Ruben JK, Zaharul Azri WMZ, Mor Japar Khan ESK

Med J Malaysia, 2019 Oct;74(5):394-399.
PMID: 31649215

INTRODUCTION: Diabetic foot infection, a complication that is associated with lower-limb amputation, incurs a huge economic burden to the hospital and health care system of Malaysia. The bacteriological profile of pathogens in diabetic foot infections in Malaysia has been sparsely studied. We investigated the microbiology of diabetic foot infections in patients admitted to the district hospitals on the east coast of Malaysia.

METHODS: A retrospective analysis was conducted in three district hospitals (Hospital Kuala Lipis, Hospital Bentong and Hospital Raub) in Malaysia from 1st of January 2016 to 31st December 2016. The clinical specimens were cultured using Clinical and Laboratory Standards Institute (CLSI) guidelines. Antibiotic sensitivity testing to different antibiotics was carried out using the disc diffusion method.

RESULT: A total of 188 pathogens were isolated from 173 patients, with an average of 1.09 pathogens per lesion. Majority of the pathogens isolated were gram negative pathogens (73.4%). The most commonly isolated pathogens were Staphylococcus aureus (17.5%). This was followed by Klebsiella spp. (17%), Pseudomonas spp. (15.4%) and Proteus spp. (13.8%). Gram positive pathogens were sensitive to most of the antibiotics tested except penicillin and fusidic acid. Gram negative pathogens were sensitive to all antibiotics tested except ampicillin and amoxicillin/clavulanic acid. Amikacin provide coverage for all gram negative pathogens in DFI.

CONCLUSION: For the management of patient with infection in diabetic foot, the choice of antibiotic therapy depends on the sensitivity of the pathogens, the severity of the infection, the patient's allergies history, toxicity and excretion of the antibiotics.
Understanding the genetic relationships between Indonesian bambara groundnut landraces and investigating their origins

Redjeki ES, Ho WK, Shah N, Molosiwa OO, Ardiarini NR, Kuswanto, et al.

Genome, 2020 Jun;63(6):319-327.
PMID: 32097026 DOI: 10.1139/gen-2019-0137

A total of 170 bambara groundnut (Vigna subterranea) accessions were evaluated using both simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers generated using genotyping-by-sequencing (GbS), of which 56 accessions were collected from West and East Java. Principal coordinate analysis (PCoA), population structure, and cluster analysis suggest that the East Java accessions could be a result of the introduction of selected West Java accessions. In addition, the current Indonesian accessions were likely introduced from Southern Africa, which would have produced a very marked founding effect such that these accessions present only a fraction of the genetic variability that exists within this species.

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