Displaying publications 121 - 140 of 150 in total

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  1. Biologic interaction of three-dimensional periodontal fibroblast spheroids with collagen-based and synthetic membranes
    Berahim Z, Moharamzadeh K, Rawlinson A, Jowett AK
    J. Periodontol., 2011 May;82(5):790-7.
    PMID: 21080786 DOI: 10.1902/jop.2010.100533
    Cell-based therapy using autologous cells has been suggested as a potential approach for periodontal tissue regeneration. Spheroid systems are a form of three-dimensional cell culture that promotes cell matrix interaction, which could recapitulate the aspect of cell homeostasis in vivo. The aim of this study is to assess the interaction of periodontal fibroblast spheroids with synthetic and collagen-based membranes that have been used in guided tissue regeneration.
    Matched MeSH terms: Cell Adhesion/physiology; Cell Adhesion Molecules/analysis
  2. Human amnion as a novel cell delivery vehicle for chondrogenic mesenchymal stem cells
    Tan SL, Sulaiman S, Pingguan-Murphy B, Selvaratnam L, Tai CC, Kamarul T
    Cell Tissue Bank, 2011 Feb;12(1):59-70.
    PMID: 19953328 DOI: 10.1007/s10561-009-9164-x
    This study investigates the feasibility of processed human amnion (HAM) as a substrate for chondrogenic differentiation of mesenchymal stem cells (MSCs). HAM preparations processed by air drying (AD) and freeze drying (FD) underwent histological examination and MSC seeding in chondrogenic medium for 15 days. Monolayer cultures were used as control for chondrogenic differentiation and HAMs without cell seeding were used as negative control. Qualitative observations were made using scanning electron microscopy analysis and quantitative analyses were based on the sulfated glycosaminoglycans (GAG) assays performed on day 1 and day 15. Histological examination of HAM substrates before seeding revealed a smooth surface in AD substrates, while the FD substrates exhibited a porous surface. Cell attachment to AD and FD substrates on day 15 was qualitatively comparable. GAG were significantly highly expressed in cells seeded on FD HAM substrates. This study indicates that processed HAM is a potentially valuable material as a cell-carrier for MSC differentiation.
    Matched MeSH terms: Cell Adhesion
  3. Fulltext Changes in the vascular cell adhesion molecule-1, intercellular adhesion molecule-1 and c-reactive protein following administration of aqueous extract of piper sarmentosum on experimental rabbits fed with cholesterol diet
    Amran AA, Zakaria Z, Othman F, Das S, Al-Mekhlafi HM, Nordin NA
    Lipids Health Dis, 2011 Jan 09;10:2.
    PMID: 21214952 DOI: 10.1186/1476-511X-10-2
    BACKGROUND: Inflammation process plays an important role in the development of atherosclerosis. Hypercholesterolemia is one of the major risk factors for atherosclerosis. The present study aimed to evaluate the effect of aqueous extract of Piper sarmentosum (P.s) on inflammatory markers like vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), and C-reactive protein (CRP).

    METHODS: Forty two male New Zealand white rabbits were divided equally into seven groups; (i) C- control group fed normal rabbit chow (ii) CH- cholesterol diet (1%cholesterol) (iii) X1- 1% cholesterol with water extract of P.s (62.5 mg/kg) (iv) X2- 1% cholesterol with water extract of P.s (125 mg/kg (v) X3- 1% cholesterol with water extract of P.s (250 mg/kg) (vi) X4- 1% cholesterol with water extract of P.s (500 mg/kg) and (vii) SMV group fed with 1% cholesterol supplemented with simvistatin drug (1.2 mg/kg). All animals were treated for 10 weeks. Blood serum was taken for observing the inflammatory markers at the beginning and end of the experiment.

    RESULTS: Rabbits fed with 1% cholesterol diet (CH) showed significant increase in the level of VCAM-1, ICAM-1 and CRP compared to the C group. The levels of VCAM-1, ICAM-1 and CRP in the 1% cholesterol group and supplemented with P.s (500 mg/kg) were significantly reduced compared to the cholesterol group. Similar results were also reported with simvistatin group.

    CONCLUSION: These results suggest that the supplementation of Piper sarmentosum extract could inhibit inflammatory markers which in turn could prevent atherosclerosis.

    Matched MeSH terms: Vascular Cell Adhesion Molecule-1/blood*
  4. Kesan masa pengeraman nanozarah zink oksida yang dihasilkan menggunakan afrons gas koloid
    Saifful Kamaluddin Muzakir, Shahidan Radiman
    Sains Malaysiana, 2011;40:1123-1127.
    Nanozarah zink oksida telah disintesis menggunakan afrons gas koloid sebagai acuan. Zink sulfat (ZnSO4.7H2O) dan gas ammonia digunakan sebagi bahan tindak balas. Masa pengeraman yang dikaji adalah 2 jam dan 18 jam. Daripada analisis mikroskop elektron imbasan, morfologi nanohelaian dapat diperhatikan dengan ketebalan helaian 125 nm hingga 200 nm. Daripada analisis spektroskopi ultra lembayung-boleh nampak, saiz purata yang dianggarkan bagi sampel nanozarah zink oksida yang disintesis dengan masa pengeraman 2 jam adalah 2.03 nm dan 2.1 nm untuk sampel yang dieramkan selama 18 jam.
    Matched MeSH terms: Cell Adhesion Molecules
  5. Development of a novel model for the investigation of implant-soft tissue interface
    Chai WL, Moharamzadeh K, Brook IM, Emanuelsson L, Palmquist A, van Noort R
    J. Periodontol., 2010 Aug;81(8):1187-95.
    PMID: 20450401 DOI: 10.1902/jop.2010.090648
    In dental implant treatment, the long-term prognosis is dependent on the biologic seal formed by the soft tissue around the implant. The in vitro investigation of the implant-soft tissue interface is usually carried out using a monolayer cell-culture model that lacks a polarized-cell phenotype. This study developed a tissue-engineered three-dimensional oral mucosal model (3D OMM) to investigate the implant-soft tissue interface.
    Matched MeSH terms: Cell Adhesion
  6. Fulltext Proliferative activity of cells from remaining dental pulp in response to treatment with dental materials
    Lutfi AN, Kannan TP, Fazliah MN, Jamaruddin MA, Saidi J
    Aust Dent J, 2010 Mar;55(1):79-85.
    PMID: 20415916 DOI: 10.1111/j.1834-7819.2009.01185.x
    The biological examination of pulp injury, repair events and response of dental pulp stem cells to dental restorative materials is important to accomplish restorative treatment, especially to commonly used dental materials in paediatric dentistry, such as glass ionomer cement (GIC) and calcium hydroxide (Ca(OH)(2)) lining cement.
    Matched MeSH terms: Cell Adhesion Molecules, Neuronal/analysis
  7. Fulltext Production of Newcastle disease virus by Vero cells grown on cytodex 1 microcarriers in a 2-litre stirred tank bioreactor
    Arifin MA, Mel M, Abdul Karim MI, Ideris A
    J Biomed Biotechnol, 2010;2010:586363.
    PMID: 20625497 DOI: 10.1155/2010/586363
    The aim of this study is to prepare a model for the production of Newcastle disease virus (NDV) lentogenic F strain using cell culture in bioreactor for live attenuated vaccine preparation. In this study, firstly we investigated the growth of Vero cells in several culture media. The maximum cell number was yielded by culture of Vero cells in Dulbecco's Modified Eagle Medium (DMEM) which was 1.93 x 10(6) cells/ml. Secondly Vero cells were grown in two-litre stirred tank bioreactor by using several commercial microcarriers. We achieved the maximum cell concentration about 7.95 x 10(5) cells/ml when using Cytodex 1. Later we produced Newcastle Disease virus in stirred tank bioreactor based on the design developed using Taguchi L4 method. Results reveal that higher multiplicity of infection (MOI) and size of cell inoculums can yield higher virus titer. Finally, virus samples were purified using high-speed centrifugation based on 3( * *)(3-1) Fractional Factorial Design. Statistical analysis showed that the maximum virus titer can be achieved at virus sample concentration of 58.45% (v/v), centrifugation speed of 13729 rpm, and centrifugation time of 4 hours. As a conclusion, high yield of virus titer could be achieved through optimization of cell culture in bioreactor and separation by high-speed centrifugation.
    Matched MeSH terms: Cell Adhesion/drug effects
  8. Fulltext The effects of short-term, rapid glycemic control on the peroneal nerve function and serum VCAM-1 and AGE in type 2 diabetic patients in Malaysia
    Norlinah MI, Hamizah R, Md Isa SH, Wan Nazaimoon WM, Khalid BA
    Indian J Med Sci, 2009 Apr;63(4):131-8.
    PMID: 19414982
    BACKGROUND: The role of endothelial injury and circulating adhesion molecule in the development and progression of diabetic peripheral neuropathy in the long-term has been established previously.
    AIMS: To study the effects of short-term glycemic control using insulin and oral hypoglycemic agent therapy (OHA) on the peroneal nerve function and vascular cell adhesion molecule-1 (VCAM-1) and advanced glycation endproducts (AGE) levels in type 2 diabetic patients.
    SETTINGS AND DESIGN: A randomized controlled study involving poorly controlled (HbA1c, 7.5%-11%) type 2 diabetic patients attending the endocrinology outpatient center in a tertiary hospital in Kuala Lumpur.
    MATERIALS AND METHODS: Twenty-nine patients were randomized to receive insulin (n=15) or OHA (n=14) for 8 weeks. The glycemic variables (HbA1c, fasting plasma glucose [FPG], fructosamine), VCAM-1, serum AGE and the peroneal motor conduction velocity (PMCV) were measured at baseline and at 4-week intervals.
    STATISTICAL ANALYSIS USED: Paired 't' test or Kruskal Wallis test; and the unpaired 't' test or Mann-Whitney U test were used for within-group and between-group analyses, respectively. Correlation was analyzed using Spearman's correlation coefficient.
    RESULTS: Within-group analysis showed significant progressive improvement in HbA1c at weeks 4 and 8 in the insulin group. The PMCV improved significantly in both groups by week 8, and by week 4 (P = 0.01) in the insulin group. PMCV correlated negatively with VCAM-1 (P = 0.031) and AGE (P = 0.009) at week 8.
    CONCLUSION: Aggressive glycemic control with insulin improves the peroneal nerve function within 4 weeks. Improvement in the serum VCAM-1 and AGE levels correlated significantly with improvement in peroneal nerve conduction velocity only in the insulin group.
    Study site: Tertiary endocrinology outpatient center in Kuala Lumpur, Malaysia
    Matched MeSH terms: Vascular Cell Adhesion Molecule-1/blood; Vascular Cell Adhesion Molecule-1/drug effects
  9. Fulltext The role of cells, neurotrophins, extracellular matrix and cell surface molecules in peripheral nerve regeneration
    Naidu M
    Malays J Med Sci, 2009 Apr;16(2):10-4.
    PMID: 22589652 MyJurnal
    Wallerian degeneration is a complicated process whereby axons and myelin sheaths undergo degeneration, and eventually are phagocytosed by macrophages and Schwann cells following nerve damage. Schwann cells proliferate and the endoneural tubes persist. In addition, neurotrophins, neural cell adhesion molecules, cytokines and other soluble factors are upregulated to facilitate regeneration. The important role of cellular components, neurotrophins, and extracellular matrix components, including cell surface molecules involved in this regenerative process, is highlighted and discussed in this review.
    Matched MeSH terms: Neural Cell Adhesion Molecules
  10. Fulltext Major cellular events in peripheral nerve regeneration: A brief overview
    Naidu, M., David, P.
    International Medical Journal Malaysia, 2009;8(1):61-64.
    MyJurnal
    Injury to a peripheral nerve leads to degeneration of the segment distal to the site of lesion, a process referred to as Wallerian degeneration. During Wallerian degeneration, axons and myelin sheaths undergo degeneration and are phagocytosed by macrophages and Schwann cells. The Schwann cells proliferate and the endoneurial tubes persist, together the whole structure is known as the band of Büngner. Within few hours, the damaged axons in the proximal stump initiate a regeneration response, with formation of new growth cones. During Wallerian degeneration, neurotrophins, neural cell adhesion molecules, cytokines and other soluble factors are upregulated to facilitate regeneration. The recovery of the target in mammals is often variable, but almost never complete. In humans, scar tissue forms at the site of lesion and this often results in poor recovery of the target. The major events underlying this regenerative process is highlighted and discussed in this review.
    Matched MeSH terms: Neural Cell Adhesion Molecules
  11. In-vitro expression of adhesion molecules and bone specific markers are depending on the cell culture material
    Salin N, Ishak AK, Abdul Rahman S, Ali M, Nawawi HM, Said MS, et al.
    Med J Malaysia, 2008 Jul;63 Suppl A:67-8.
    PMID: 19024987
    Bone formation is an active process whereby osteoblasts are found on the surface of the newly formed bone. Adhesion to extracellular matrix is essential for the development of bone however not all surfaces are suitable for osteoblast adhesion and don't support osteoblastic functions. The objective of this study was to test the suitability of a collagen based microcarrier which would support osteoblastic functions.
    Matched MeSH terms: Cell Adhesion Molecules/physiology*
  12. Cell attachment study of chicken fibroblast cell (DF-1) using ceramic microcarrier granule in bioreactors
    Mel M, Sopyan I, Nor YA
    Med J Malaysia, 2008 Jul;63 Suppl A:18-20.
    PMID: 19024963
    Tricalcium phosphate ceramic microcarrier has been developed and introduced to a new possibility for the culture of anchorage dependent animal cells of DF1. It was observed that the number of attached cells was increased with shorter time for both spinner vessel and stirred tank (ST) bioreactor. For those bioreactors, the total viable cell number that had been obtained is about 1.2 x 10(5) cell/ml.
    Matched MeSH terms: Cell Adhesion
  13. Mouse bone marrow mesenchymal stem cells acquire CD45-CD106+ immunophenotype only at later passages
    Ooi YY, Ramasamy R, Vidyadaran S
    Med J Malaysia, 2008 Jul;63 Suppl A:65-6.
    PMID: 19024986
    Classically, MSC are identified by a CD45-CD106+ phenotype. In this study, we found that mouse MSC achieve this characteristic phenotype only at later passages. With increasing passages, CD45 (hematopoietic marker) expression shifts to negativity, whereas CD106 (vascular cell adhesion molecule-1) expression becomes increasingly positive. These results demonstrate that MSC cells cultured from mouse bone marrow acquire a classical MSC immunophenotype (CD45-CD106+) in later passages.
    Matched MeSH terms: Vascular Cell Adhesion Molecule-1/genetics*
  14. Fulltext Small cell variant of anaplastic large cell lymphoma with positive immunoreactivity for CD99
    Shiran MS, Tan GC, Sabariah AR, Chye PC, Pathmanathan R
    Med J Malaysia, 2008 Jun;63(2):150-1.
    PMID: 18942305 MyJurnal
    A 13 year old boy presented with a huge mass on his right arm of 6 months duration. Histopathological examination revealed sheets of malignant small round blue cells with immunopositivity for LCA, CD43, CD45Ro, CD30, EMA, ALK-1 and CD99, and negativity for CD20, TdT, myogenin, myoD1, NSE, bcl-6, bcl-2 and CD10. Fluorescent In-Situ Hybridization (FISH) testing excluded the diagnosis of Ewing's sarcoma/PNET. Pathologists need to be aware of the diagnosis of a small cell variant of ALCL, as well as of the fact that CD99 expression commonly occurs in cases of ALK-positive ALCL, in order to distinguish this entity from Ewing's sarcoma/PNET.
    Matched MeSH terms: Cell Adhesion Molecules/immunology*
  15. Primitive neuroectodermal tumor of the lung with pericardial extension: a case report
    Shah Mohd Shah A, Mohamed Z, Abdullah A, Abdul Malek PM, Saidin N, Maskon O
    Cardiovasc. Pathol., 2007 Nov-Dec;16(6):351-3.
    PMID: 18005874
    A 16-year-old student presented with a 4-week history of progressive shortness of breath, loss of appetite, and occasional blood-tinged sputum. The chest X-ray revealed massive right-sided pleural effusion with cardiomegaly. An echocardiogram revealed a large pericardial mass with massive pericardial effusion. Subsequent computed tomography of the thorax revealed a large heterogeneous mass in the right lung with extension into the pericardium. Lung biopsy revealed primitive neuroectodermal tumor (PNET) with small round blue cells, Homer-Wright rosettes, and CD99 positivity. We discuss pericardial metastases of PNET and its implication in this patient.
    Matched MeSH terms: Cell Adhesion Molecules/analysis
  16. Effects of fluoride-modified titanium surfaces on osteoblast proliferation and gene expression
    Isa ZM, Schneider GB, Zaharias R, Seabold D, Stanford CM
    Int J Oral Maxillofac Implants, 2006 Mar-Apr;21(2):203-11.
    PMID: 16634490
    PURPOSE: The objective of this study was to test the hypothesis that fluoride-modified titanium surfaces would enhance osteoblast differentiation. Osteoblast growth on a moderately rough etched fluoride-modified titanium surface (alteration in cellular differentiation) was compared to osteoblast growth on the same surface grit-blasted with titanium dioxide. The potential role of nanometer-level alterations on cell shape and subsequent differentiation was then compared.
    MATERIALS AND METHODS: Human embryonic palatal mesenchymal (HEPM) cultures were incubated on the respective surfaces for 1, 3, and 7 days, followed by analysis for cell proliferation, alkaline phosphatase (ALP) -specific activity, and mRNA steady-state expression for bone-related genes (ALP, type I collagen, osteocalcin, bone sialoprotein [BSP] II, Cbfa1, and osterix) by real-time polymerase chain reaction (PCR).
    RESULTS: The different surfaces did not alter the mRNA expression for ALP, type I collagen, osterix, osteocalcin, or BSP II. However, Cbfa1 expression on the fluoride-modified titanium surface was significantly higher (P < .001) at 1 week. The number of cells on this surface was 20% lower than the number of cells on the surface TiO2-blasted with 25-microm particles but not significantly different from the number of cells on the surface TiO2-blasted with 125-microm particles. Cells grown on all the titanium surfaces expressed similar levels of ALP activity.
    CONCLUSIONS: The results indicated that a fluoride-modified surface topography, in synergy with surface roughness, may have a greater influence on the level of expression of Cbfa1 (a key regulator for osteogenesis) than the unmodified titanium surfaces studied.
    Matched MeSH terms: Cell Adhesion/drug effects*
  17. Comparison of chitosan scaffold and chitosan-collagen scaffold: a preliminary study
    Norazril SA, Aminuddin BS, Norhayati MM, Mazlyzam AL, Fauziah O, Ruszymah BH
    Med J Malaysia, 2004 May;59 Suppl B:186-7.
    PMID: 15468880
    Chitosan has similar structure to glycosaminoglycans in the tissue, thus may be a good candidates as tissue engineering scaffold. However, to improve their cell attachment ability, we try to incorporate this natural polymer with collagen by combining it via cross-linking process. In this preliminary study we evaluate the cell attachment ability of chitosan-collagen scaffold versus chitosan scaffold alone. Chitosan and collagen were dissolved in 1% acetic acid and then were frozen for 24 hours before the lyophilizing process. Human skin fibroblasts were seeded into both scaffold and were cultured in F12: DMEM (1:1). Metabolic activity assay were used to evaluate cell attachment ability of scaffold for a period of 1, 3, 7 and 14 days. Scanning electron micrographs shows good cell morphology on chitosan-collagen hybrid scaffold. In conclusion, the incorporation of collagen to chitosan will enhance its cell attachment ability and will be a potential scaffold in tissue engineering.
    Matched MeSH terms: Cell Adhesion/physiology*
  18. Evaluation of suitable biodegradable scaffolds for engineered bone tissue
    Phang MY, Ng MH, Tan KK, Aminuddin BS, Ruszymah BH, Fauziah O
    Med J Malaysia, 2004 May;59 Suppl B:198-9.
    PMID: 15468886
    Tricalcium phosphate/hydroxyapatite (TCP/HA), hydroxyapatite (HA), chitosan and calcium sulphate (CaSO4) were studied and evaluated for possible bone tissue engineered construct acting as good support for osteogenic cells to proliferate, differentiate, and eventually spread and integrate into the scaffold. Surface morphology visualized by SEM showed that scaffold materials with additional fibrin had more cell densities attached than those without, depicting that the presence of fibrin and collagen fibers were truly a favourite choice of cells to attach. In comparison of various biomaterials used incorporated with fibrin, TCP/HA had the most cluster of cells attached.
    Matched MeSH terms: Cell Adhesion/physiology*
  19. Collagen as potential cell scaffolds for tissue engineering
    Annuar N, Spier RE
    Med J Malaysia, 2004 May;59 Suppl B:204-5.
    PMID: 15468889
    Selections of collagen available commercially were tested for their biocompatibility as scaffold to promote cell growth in vitro via simple collagen fast test and cultivation of mammalian cells on the selected type of collagen. It was found that collagen type C9791 promotes the highest degree of aggregation as well as cells growth. This preliminary study also indicated potential use of collagen as scaffold in engineered tissue.
    Matched MeSH terms: Cell Adhesion/physiology*
  20. Coral--polyhydroxybutrate composite scaffold for tissue engineering: prefabrication properties
    Al-Salihi KA, Samsudin AR
    Med J Malaysia, 2004 May;59 Suppl B:202-3.
    PMID: 15468888
    In this study the surface properties of two particulate coral and polyhydroxybutrate (PHB) were studied in order to characterize them prior to use in composite production. Coral powder and PHB particle were evaluated using scanning electron microscopy and confocal laser scanning microscopy, to measure surface porosity and pores size. The results showed that coral powder has multiple pleomorphic micropores cross each others give appearance of micro-interconnectivity. Some pore reached to 18 microm with an average porosity of 70%. PHB revealed multiple different size pores extended to the depth, with an average some times reach 25 microm and porosity 45%. These findings demonstrate that both coral and PHB have excellent pores size and porosity that facilitate bone in growth, vascular invasion and bone development. We believe that incorporation of coral powder into PHB will make an excellent composite scaffold for tissue engineering.
    Matched MeSH terms: Cell Adhesion/physiology*
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