Displaying publications 1 - 20 of 29 in total

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  1. Ho HC, Loh KH
    Zootaxa, 2015;4060:52-5.
    PMID: 26701590 DOI: 10.11646/zootaxa.4060.1.8
    A new ophichthid worm-eel, Neenchelys gracilis sp. nov., is described from a specimen collected from southwestern Taiwan. It differs from its congeners by having: a minute pectoral fin, many filamentous cirri on the anterior nostril rim; a very slender body; a very small gill opening; and a vertebral formula of 30-78-200.
  2. Ramos-Castro M, Loh KH, Chen HM
    Zootaxa, 2021 Aug 20;5023(4):509-536.
    PMID: 34810950 DOI: 10.11646/zootaxa.5023.4.3
    Taiwan is one of the richest in the world in terms of eel fauna. In this study, we examined the osteological and morphological characteristics of eels under order Anguilliformes. Furthermore, we focused on the neurocranium of total of 30 Anguilliformes fishes under family Congridae (10), Muraenesocidae (1), Muraenidae (7), Nemichthyidae (1), Nettastomatidae (2), Ophichthidae (5), Synaphobranchidae (4), which are caught in Taiwanese waters. This paper shows the results of a comparative study on osteological characters of the neurocranium including the ratio of seven length characters using its NCL (neurocranium length), NCW (neurocranium width), OBL (orbit length), MFW (maximum frontal width), NCDB (neurocranium depth at basisphenoid), PEVW (premaxilla-ethmovomer width) and mPOBL (mid pre-orbital length), and 20 morphological diagnostic characters for 30 eel species. Results shows that species under family Nemichthyidae and Nettastomatidae have the highest values on the ratio of NCL/MFW, NCL/NCDB, and NCW/mPOBD. In morphological characters, it shows that species of the same family mostly share similar formation of the PEV plate and frontal structure. The usage of the length measurements and morphological diagnostic characters of neurocranium allowed for a more in depth understanding of how similar or different these eels can be. The neurocranial description and morphological characters may prove valuable for identification purposes and might be necessary tool for further studies on the status of order Anguilliformes.
  3. Huang WC, Loh KH, Chen HM
    Zootaxa, 2022 Sep 23;5189(1):87-102.
    PMID: 37045199 DOI: 10.11646/zootaxa.5189.1.11
    Gymnothorax poikilospilus sp. nov. is described based on two specimens collected from Penghu Islands, western Taiwan. It is a medium-sized brown moray that body covered with several rows of inconspicuous large dark brown patches on the back of body and dorsal fin. It has slightly elongated and arched jaws similar to the common characteristic of the genus Enchelycore Kaup, but the dentition supports it belongs to typical morays of the genus Gymnothorax Bloch. The new species can be distinguished from other similar Indo-Pacific brown morays by the combination of dentition, vertebral formula, and morphometric measurements. Molecular analyses based on 612 bp of mitochondrial COI gene also support it as a distinct species.
  4. Salimi N, Loh KH, Kaur Dhillon S, Chong VC
    PeerJ, 2016;4:e1664.
    PMID: 26925315 DOI: 10.7717/peerj.1664
    Background. Fish species may be identified based on their unique otolith shape or contour. Several pattern recognition methods have been proposed to classify fish species through morphological features of the otolith contours. However, there has been no fully-automated species identification model with the accuracy higher than 80%. The purpose of the current study is to develop a fully-automated model, based on the otolith contours, to identify the fish species with the high classification accuracy. Methods. Images of the right sagittal otoliths of 14 fish species from three families namely Sciaenidae, Ariidae, and Engraulidae were used to develop the proposed identification model. Short-time Fourier transform (STFT) was used, for the first time in the area of otolith shape analysis, to extract important features of the otolith contours. Discriminant Analysis (DA), as a classification technique, was used to train and test the model based on the extracted features. Results. Performance of the model was demonstrated using species from three families separately, as well as all species combined. Overall classification accuracy of the model was greater than 90% for all cases. In addition, effects of STFT variables on the performance of the identification model were explored in this study. Conclusions. Short-time Fourier transform could determine important features of the otolith outlines. The fully-automated model proposed in this study (STFT-DA) could predict species of an unknown specimen with acceptable identification accuracy. The model codes can be accessed at http://mybiodiversityontologies.um.edu.my/Otolith/ and https://peerj.com/preprints/1517/. The current model has flexibility to be used for more species and families in future studies.
  5. Lim KC, Lim PE, Chong VC, Loh KH
    PLoS One, 2015;10(4):e0120518.
    PMID: 25867639 DOI: 10.1371/journal.pone.0120518
    Elucidating the phylogenetic relationships of the current but problematic Dasyatidae (Order Myliobatiformes) was the first priority of the current study. Here, we studied three molecular gene markers of 43 species (COI gene), 33 species (ND2 gene) and 34 species (RAG1 gene) of stingrays to draft out the phylogenetic tree of the order. Nine character states were identified and used to confirm the molecularly constructed phylogenetic trees. Eight or more clades (at different hierarchical level) were identified for COI, ND2 and RAG1 genes in the Myliobatiformes including four clades containing members of the present Dasyatidae, thus rendering the latter non-monophyletic. The uncorrected p-distance between these four 'Dasytidae' clades when compared to the distance between formally known families confirmed that these four clades should be elevated to four separate families. We suggest a revision of the present classification, retaining the Dasyatidae (Dasyatis and Taeniurops species) but adding three new families namely, Neotrygonidae (Neotrygon and Taeniura species), Himanturidae (Himantura species) and Pastinachidae (Pastinachus species). Our result indicated the need to further review the classification of Dasyatis microps. By resolving the non-monophyletic problem, the suite of nine character states enables the natural classification of the Myliobatiformes into at least thirteen families based on morphology.
  6. Li Y, Liu C, Lin L, Li Y, Xiao J, Loh KH
    Zookeys, 2020;969:137-154.
    PMID: 33013170 DOI: 10.3897/zookeys.969.52069
    The southern lesser pomfret (Pampus minor) is an economically important fish, and its numbers are declining because of overfishing and environmental pollution. In addition, owing to the similarities of its external morphological characteristics to other species in the genus Pampus, it is often mistaken for grey pomfret (P. cinereus) or silver pomfret (P. argenteus) juveniles. In this study, the genetic diversity and structure of 264 P. minor individuals from 11 populations in China and Malaysia coastal waters were evaluated for the first time, to the best of our knowledge, using mitochondrial cytochrome b fragments. The results showed that P. minor had moderate haplotype diversity and low nucleotide diversity. Furthermore, two divergent lineages were detected within the populations, but the phylogenetic structure corresponded imperfectly with geographical location; thus, the populations may have diverged in different glacial refugia during the Pleistocene low sea levels. Analysis of molecular variation (AMOVA) showed that genetic variation originated primarily from individuals within the population. Pairwise FST results showed significant differentiation between the Chinese and Malaysian populations. Except for the Xiamen population, which was classified as a marginal population, the genetic differentiation among the other Chinese populations was not significant. During the Late Pleistocene, P. minor experienced a population expansion event starting from the South China Sea refugium that expanded outward, and derivative populations quickly occupied and adapted to the new habitat. The results of this study will provide genetic information for the scientific conservation and management of P. minor resources.
  7. Wong JY, Chu C, Chong VC, Dhillon SK, Loh KH
    J Fish Biol, 2016 Aug;89(2):1324-44.
    PMID: 27364089 DOI: 10.1111/jfb.13039
    Combined multiple 2D views (proximal, anterior and ventral aspects) of the sagittal otolith are proposed here as a method to capture shape information for fish classification. Classification performance of single view compared with combined 2D views show improved classification accuracy of the latter, for nine species of Sciaenidae. The effects of shape description methods (shape indices, Procrustes analysis and elliptical Fourier analysis) on classification performance were evaluated. Procrustes analysis and elliptical Fourier analysis perform better than shape indices when single view is considered, but all perform equally well with combined views. A generic content-based image retrieval (CBIR) system that ranks dissimilarity (Procrustes distance) of otolith images was built to search query images without the need for detailed information of side (left or right), aspect (proximal or distal) and direction (positive or negative) of the otolith. Methods for the development of this automated classification system are discussed.
  8. Huang WC, Smith DG, Loh KH, Liao TY
    Zool Stud, 2021;60:e24.
    PMID: 34853614 DOI: 10.6620/ZS.2021.60-24
    Two new moray eels of the genera Diaphenchelys and Gymnothorax from Taiwan and the Philippines are described. Diaphenchelys laimospila sp. nov. is described based on two specimens that represent the third species and a new geographic record of the genus. It can be distinguished from the other two congeners by the number of cephalic sensory pores, vertebral formula, morphometric measurements, and the coloration pattern. Gymnothorax pseudokidako sp. nov. is a muraenid with a dark brown body covered by pale snowflake-like blotches. It differs from the most similar species Gymnothorax kidako (Temminck and Schlegel) by having a relatively short tail (50.5-53.0% vs. 52.9-56.4% of TL), more dentary teeth (17-26 vs. 16-20), fewer total vertebrae (134-139 vs. 137-143), and the absence of white margin on anal fin (vs. prominent white margin). These two new species were also confirmed by molecular analyses, the mitochondrial COI gene (593 bp) for D. laimospila, and the nuclear EGR3 gene (767 bp) for G. pseudokidako.
  9. Shen KN, Loh KH, Chen CH, Hsiao CD
    Mitochondrial DNA A DNA Mapp Seq Anal, 2016 11;27(6):4122-4123.
    PMID: 25585497
    In this study, the complete mitogenome sequence of the Blue-face angelfish, Pomacanthus xanthometapon (Perciformes: Pomacanthidae) has been sequenced by the next-generation sequencing method. The assembled mitogenome consisting of 16,533 bp includes 13 protein coding genes, 22 transfer RNAs, and two ribosomal RNAs genes. The overall base composition of Blue-face angelfish is 28.7% for A, 28.9% for C, 15.9% for G, 26.6% for T and show 84% identities to flame angelfish Centropyge loriculus. The complete mitogenome of the Blue-face angelfish provides essential and important DNA molecular data for further phylogeography and evolutionary analysis for marine angelfish phylogeny.
  10. Leung AJ, Then AY, Loh KH
    J Fish Biol, 2023 Mar;102(3):564-574.
    PMID: 36504128 DOI: 10.1111/jfb.15288
    Recent IUCN assessments had resulted in up listing of the status of butterfly rays due to concerns of overfishing, but inadequate biological understanding of these rays prevents meaningful conservation and management measures. Therefore, this study was undertaken to address knowledge gaps in the reproductive biology and diet of longtail butterfly ray (Gymnura poecilura) and zone tail butterfly ray (Gymnura zonura) in Malaysian waters. From surveys of landing sites and fish markets from years 2017 to 2022, size (disc width, DW), weight and maturity were recorded, and stomachs were collected from 94 G. poecilura (N = 39 females and 55 males) and 20 G. zonura (N = 10 females and 10 males) specimens. The length-weight relationships were significantly different between sexes for G. poecilura. The size at maturity (DW50) was estimated to be 476.0 mm (females), 385.0 mm (males) for G. poecilura and 442.0 mm (combined) for G. zonura. The number of embryos ranged from 1 to 6, and the embryo size was between 73.90 to 130.44 mm DW. Dietary analysis of stomach contents revealed that fish prey was dominant in both G. poecilura [94.4% Index of Relative Importance (IRI)] and G. zonura (100% IRI). Ontogenetic shift was seen in G. poecilura that fed on more variety of prey items, including shrimps, squids and crabs with an increase in body size. Both species co-occur all along coastal Malaysia although G. zonura is rarely encountered from fisheries surveys along the Strait of Malacca. Given similar habitat associations and dietary habits, G. poecilura may be able to outcompete G. zonura across their shared habitat range. The validity of G. japonica and G. micrura records in Malaysia remains questionable and requires future investigation.
  11. Lim KC, Then AY, Loh KH
    PeerJ, 2023;11:e15849.
    PMID: 37637173 DOI: 10.7717/peerj.15849
    Small coastal demersal sharks form a major proportion of the sharks landed in Malaysia. However, little is known about their feeding ecology and reproduction. This study sought to elucidate the dietary patterns, role of ontogeny in prey consumption, and reproductive biology of four dominant small demersal shark species in Malaysian waters: the Hasselt's bamboo shark, Chiloscyllium hasseltii; brownbanded bamboo shark, C. punctatum; spadenose shark, Scoliodon laticaudus; and Pacific spadenose shark, S. macrorhynchos. Dietary analyses revealed a high overlap in prey taxa consumed; clear resource partitioning among co-occurring species based on the percentage Prey-specific Index of Relative Importance (%PSIRI), with higher fish %PSIRI for Chiloscyllium hasseltii, higher cephalopod %PSIRI for C. punctatum, and higher crustacean %PSIRI for both Scoliodon species; and an ontogenetic diet shift, seen through changes in prey size. Based on the examination of reproductive organs, the results showed larger sizes at maturity for males compared to females for all four species; no obvious reproductive cycles, based on hepatosomatic and gonadosomatic indices for all species; female bias in the sex ratio of the embryos of Scoliodon species; and increased reproductive output (number of eggs or embryos and size of eggs) with larger female size for C. hasseltii and Scoliodon species. The partitioning of food resources minimizes direct competition for food and supports coexistence within shared coastal habitats. The reproductive strategies of these small coastal sharks appear to be favorable for supporting short-term population productivity; although a reduction in fishing pressure, especially from bottom trawlers, is essential for the long-term sustainable use of these sharks.
  12. Loh KH, Shao KT, Ho HC, Lim PE, Chen HM
    Zootaxa, 2015;4060:30-40.
    PMID: 26701587 DOI: 10.11646/zootaxa.4060.1.5
    The following nine elongate unpatterned muraenid species of the subfamily Muraeninae, including one new species, are recognized from Taiwan and adjacent waters: Gymnothorax albimarginatus (Temminck & Schlegel), G. dorsalis Seale, G. melanosomatus Loh, Shao & Chen, G. phasmatodes (Smith), G. prolatus Sasaki & Amaoka, G. sagmacephalus Böhlke, Pseudechidna brummeri (Bleeker), Strophidon sathete (Hamilton) and G. pseudomelanosomatus new species, described from two specimens. This new moray eel is distinguished from its similar species, G. melanosomatus, by the following features: grey brown body (vs. black), snout length 20.5% (vs. 17.8%) of head length, smaller eye diameter 8.2% (vs. 10.0%) of head length; preanal length 49.5% (vs. 58.5%) total length, and preanal vertebrae 89-89 (vs. 105-109). Phylogenetic relationships of the nine species were examined using nucleotide sequence data from partial sequences of mitochondrial ND5 gene (600 bp), and seven species form COI (600 bp). The genetic analyses suggest that G. pseudomelanosomatus is distinct from G. melanosomatus and the other six species of Gymnothorax. Morphological features and mitogenetic affinities strongly suggest that "G." dorsalis should be placed in Strophidon rather than in Gymnothorax. The results also suggest that employment of ND5 and COI gene sequences are rather useful for identification of species and for obtaining reasonable insights into the phylogeny of the muraenid species.
  13. Ho HC, Smith DG, Mccosker JE, Hibino Y, Loh KH, Tighe KA, et al.
    Zootaxa, 2015;4060:140-89.
    PMID: 26701598 DOI: 10.11646/zootaxa.4060.1.16
    An annotated checklist of eels, orders Anguilliformes and Saccopharyngiformes, occurring in Taiwanese waters is presented. The checklist is the result of a series of systematic studies conducted by the authors in the past few years. The eel fauna of Taiwan is one of the richest in the world with a total of 206 species in 74 genera and 13 families in Anguilliformes and a single species in Saccopharyngiformes. The most species-rich families are the Muraenidae with 71 species, followed by the Ophichthidae with 60 species, the Congridae with 29 species, and the Synaphobranchidae with 17 species. Moreover, three genera and 42 species have been described based on at least one type specimen collected from Taiwan. Of these, 36 species are recognized as valid and 23 species are known only from Taiwanese waters at present. Historical records of all Taiwanese eel species are reviewed by examining the original descriptions and figures, vouchers, as well as the recently collected specimens, where available. This represents the first detailed checklist of eels from Taiwanese waters.
  14. Chew LL, Chong VC, Wong RCS, Lehette P, Ng CC, Loh KH
    Mar Pollut Bull, 2015 Dec 15;101(1):69-84.
    PMID: 26581817 DOI: 10.1016/j.marpolbul.2015.11.022
    Zooplankton samples collected before (1985-86) and after (2013-14) the establishment of Kapar power station (KPS) were examined to test the hypothesis that increased sea surface temperature (SST) and other water quality changes have altered the zooplankton community structure. Elevated SST and reduced pH were detected between before and after impact pairs, with the greatest impact at the station closest to KPS. Present PAHs and heavy metal concentrations are unlikely causal factors. Water parameter changes did not affect diversity but community structure of the zooplankton. Tolerant small crustaceans, salps and larvaceans likely benefited from elevated temperature, reduced pH and shift to a more significant microbial loop exacerbated by eutrophication, while large crustaceans were more vulnerable to such changes. It is predicted that any further rise in SST will remove more large-bodied crustacean zooplankton, the preferred food for fish larvae and other meroplankton, with grave consequences to fishery production.
  15. Du J, Loh KH, Then AY, Zheng X, Teguh Peristiwady, Rizman-Idid M, et al.
    Zookeys, 2019;861:107-118.
    PMID: 31333328 DOI: 10.3897/zookeys.861.34043
    Five specimens of Epinephelusepistictus (Temminck & Schlegel, 1843) were collected from a major landing site located on the west coast of Peninsula Malaysia during a fish faunal survey on 23 August 2017. The present study extends the distribution range of E.epistictus southwards from Andaman Sea to the Strait of Malacca. Species identification was confirmed by colour pattern and DNA barcoding (567 bp of cytochrome C oxidase I) of all E.epistictus specimens and nine closely related Epinephelus species. The interspecies genetic distance ranged from 0.002-0.245. This study also presents, for the first time for Malaysia, data on length-weight relationships and otolith measurements. It contributes to a better understanding of taxonomy, and phylogenetic and genetic diversity of E.epistictus.
  16. Qiu S, Chen B, Du J, Loh KH, Liao J, Liu X, et al.
    Biodivers Data J, 2021;9:e63945.
    PMID: 33732033 DOI: 10.3897/BDJ.9.e63945
    Background: The Xisha Islands are composed of the Yongle Islands and the Xuande Islands in Hainan Province, China. It has one of the highest species diversity in the world and is also a typical oceanic distribution area of coral reefs globally. The ichthyofauna of the Xisha Islands were recorded by underwater visual census in May 2019 and July 2020. The survey data were combined with previous records of species into the checklist of the Xisha Islands presented herein. A total of 691 species, belonging to 24 orders and 97 families, was recorded. The major families were Labridae, Pomacentridae, Serranidae, Chaetodontidae, Hexanchidae, Lutjanidae, Scaridae, Gobiidae, Scorpaenidae and Carangidae. In this study, the Coral Fish iversity Index (CFDI) of six families (Chaetodontidae, Pomacanthidae, Pomacentridae, Labridae, Scaridae and Acanthuridae) was 229, indicating 756 coral fishes. In terms of the IUCN Red List, one species is Critically Endangered (Glyphis gangeticus), six species are Endangered (Stegostoma fasciatum, Aetomylaeus maculatus, Aetomylaeus vespertilio, Epinephelus akaara, Cheilinusundulatus sp. and Xiphias gladius), 16 species are Vulnerable, and 13 species are Near Threatened in the Xisha Archipelago, so conservation should be strengthened in this area in the future.

    New information: One species is a new record for China (Dischistodus pseudochrysopoecilus) and 23 species are newly found in the Xisha Islands.

  17. Tan HY, Goh ZY, Loh KH, Then AY, Omar H, Chang SW
    PeerJ, 2021;9:e11825.
    PMID: 34434645 DOI: 10.7717/peerj.11825
    Background: Despite the high commercial fisheries value and ecological importance as prey item for higher marine predators, very limited taxonomic work has been done on cephalopods in Malaysia. Due to the soft-bodied nature of cephalopods, the identification of cephalopod species based on the beak hard parts can be more reliable and useful than conventional body morphology. Since the traditional method for species classification was time-consuming, this study aimed to develop an automated identification model that can identify cephalopod species based on beak images.

    Methods: A total of 174 samples of seven cephalopod species were collected from the west coast of Peninsular Malaysia. Both upper and lower beaks were extracted from the samples and the left lateral views of upper and lower beak images were acquired. Three types of traditional morphometric features were extracted namely grey histogram of oriented gradient (HOG), colour HOG, and morphological shape descriptor (MSD). In addition, deep features were extracted by using three pre-trained convolutional neural networks (CNN) models which are VGG19, InceptionV3, and Resnet50. Eight machine learning approaches were used in the classification step and compared for model performance.

    Results: The results showed that the Artificial Neural Network (ANN) model achieved the best testing accuracy of 91.14%, using the deep features extracted from the VGG19 model from lower beak images. The results indicated that the deep features were more accurate than the traditional features in highlighting morphometric differences from the beak images of cephalopod species. In addition, the use of lower beaks of cephalopod species provided better results compared to the upper beaks, suggesting that the lower beaks possess more significant morphological differences between the studied cephalopod species. Future works should include more cephalopod species and sample size to enhance the identification accuracy and comprehensiveness of the developed model.

  18. Chu C, Loh KH, Ng CC, Ooi AL, Konishi Y, Huang SP, et al.
    Zool Stud, 2019;58:e30.
    PMID: 31966331 DOI: 10.6620/ZS.2019.58-30
    Larval descriptions of tropical marine and coastal fishes are very few, and this taxonomic problem is further exacerbated by the high diversity of fish species in these waters. Nonetheless, accurate larval identification in ecological and early life history studies of larval fishes is crucial for fishery management and habitat protection. The present study aimed to evaluate the usefulness of DNA barcodes to support larval fish identification since conventional dichotomous keys based on morphological traits are not efficient due to the lack of larval traits and the rapid morphological changes during ontogeny. Our molecular analysis uncovered a total of 48 taxa (21 families) from the larval samples collected from the Klang Strait waters encompassing both spawning and nursery grounds of marine and estuarine fishes. Thirty-two (67%) of the larval taxa were identified at the species level, two taxa (4%) at the genus level, and 14 taxa (29%) at family level. The relatively low rate of species-level identification is not necessarily due to the DNA barcoding method per se, but a general lack of reference sequences for speciose and non- commercial fish families such as Gobiidae, Blenniidae, and Callionymidae. Larval morphology remains important in species diagnoses when molecular matches are ambiguous. A lower ethanol percentage (50%) for larva preservation is also useful to keep the body of larvae intact for morphological identification, and to preserve DNA for subsequent molecular analyses. The 10% Chelex resin used to extract DNA is also cost- effective for long term monitoring of larval fishes. Hence, the DNA barcoding method is an effective and easy way to aid the identification of estuarine larval fishes at the species level.
  19. Shen KN, Chang CW, Loh KH, Chen CH, Hsiao CD
    Mitochondrial DNA A DNA Mapp Seq Anal, 2016 11;27(6):4118-4119.
    PMID: 25600747
    In this study, the complete mitogenome sequence of the Clarion angelfish, Holacanthus clarionensis (Perciformes: Pomacanthidae) has been sequenced by next-generation sequencing method. The length of the assembled mitogenome is 16,615 bp, including 13 protein coding genes, 22 transfer RNAs, and two ribosomal RNAs genes. The overall base composition of Clarion angelfish is 28.3% for A, 29.3% for C, 16.5% for G, 25.9% for T and show 85% identities to flame angelfish Centropyge loriculus. The complete mitogenome of the Clarion angelfish provides essential and important DNA molecular data for further phylogeography and evolutionary analysis for marine angelfish phylogeny.
  20. Lim KC, Then AY, Wee AKS, Sade A, Rumpet R, Loh KH
    Sci Rep, 2021 Jul 21;11(1):14874.
    PMID: 34290296 DOI: 10.1038/s41598-021-94257-7
    The demersal brown banded bamboo shark Chiloscyllium punctatum is a major component of sharks landed in Malaysia. However, little is known about their population structure and the effect of high fishing pressure on these weak swimming sharks. Both mitochondrial DNA control region (1072 bp) and NADH dehydrogenase subunit 2 (1044 bp) were used to elucidate the genetic structure and connectivity of C. punctatum among five major areas within the Sundaland region. Our findings revealed (i) strong genetic structure with little present day mixing between the major areas, (ii) high intra-population genetic diversity with unique haplotypes, (iii) significant correlation between genetic differentiation and geographical distance coupled with detectable presence of fine scale geographical barriers (i.e. the South China Sea), (iv) historical directional gene flow from the east coast of Peninsular Malaysia towards the west coast and Borneo, and (v) no detectable genetic differentiation along the coastline of east Peninsular Malaysia. Genetic patterns inferred from the mitochondrial DNA loci were consistent with the strong coastal shelf association in this species, the presence of contemporary barriers shaped by benthic features, and limited current-driven egg dispersal. Fine scale population structure of C. punctatum highlights the need to improve genetic understanding for fishery management and conservation of other small-sized sharks.
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