Displaying publications 1 - 20 of 54 in total

  1. Kundin WD, Nadchatram M, Keong A
    J Med Entomol, 1972 Dec 20;9(6):558-9.
    PMID: 4654691
    Matched MeSH terms: Larva/physiology
  2. Ara R, Arshad A, Amin SMN, Ghaffar MA
    J Environ Biol, 2016 07;37(4 Spec No):735-43.
    PMID: 28779733
    The stomach contents of Omobranchus sp. (family Blenniidae) larvae were investigated in a seagrass-mangrove based ecosystem in Johor Strait, Malaysia from October 2007 to September 2008. Specimens of larval fish were collected through subsurface towing of a Bongo net from five different stations. The stomach sacs of 267 Omobranchus sp. larvae were separated and observed, which comprised of 24 significant food stuffs belonging to 6 main groups viz. phytoplankton (62.45%), zooplankton (18.24%), algae (5.56%), plant-like particles (5.75%), debris (4.22%) and unidentified particles (2.03%). In situ water parameters were also measured throughout the sampling cruises. There was a strong and significant positive correlation between stomach phytoplankton and salinity (r = 0.658, p < 0.05).? Canonical correlation analysis indicated a weak relationship (29.8%) between stomach contents and physico-chemical parameters. Only salinity appeared to be the controlling factor for the stomach contents of Omobranchus sp. larvae in the investigated area. Based on the stomach content analysis, it could be concluded that Omobranchus sp. were mainly herbivorous during the larval stages. ?
    Matched MeSH terms: Larva/physiology
  3. Mukai Y, Sanudin N, Firdaus RF, Saad S
    Zoolog Sci, 2013 Jun;30(6):421-4.
    PMID: 23721464 DOI: 10.2108/zsj.30.421
    In general, African catfish shows higher survival rates in the dark conditions than in the light conditions. In this study, larval behavior of African catfish was observed under 0, 0.01, 0.1, 1, 10, and 100 lx using a CCD camera to investigate the reason why African catfish larvae show higher survival rates in dark conditions. The larvae showed significantly higher swimming activity under 0, 0.01, and 0.1 lx than that under 10 and 100 lx. The larvae also showed significantly increased aggressive behavior under 10 and 100 lx; the swimming larvae attacked resting individuals more frequently under 10 and 100 lx than under 0, 0.01, and 0.1 lx. The aggressive behavior and sharp teeth of the attacking larvae appeared to induce skin surface lesions on injured larvae. Chemical substances were then generated from the injured skin surface, and these chemical stimuli triggered cannibalistic behavior in other fish near the injured fish. The results of this study demonstrate that the higher survival rates of African catfish larvae under dark conditions are a result of inactivity and subsequent increase in chemical releasing stimuli concentrations around inactive individuals that triggers feeding behavior in nearby active catfish. Therefore, we recommend larval rearing of African catfish in dark or dim conditions, as it improves catfish survival rates.
    Matched MeSH terms: Larva/physiology
  4. Neoh KB, Lee CY
    Environ Entomol, 2010 Jun;39(3):835-40.
    PMID: 20550796 DOI: 10.1603/EN09212
    Misotermes mindeni Disney and Neoh is a solitary endoparasitoid of Macrotermes gilvus (Hagen) (Termitidae: Macrotermitinae), exclusively parasitizing the head of major soldiers. In this study, behavioral and morphological changes in the parasitized termite host were evaluated. We also observed the larval parasitoid emerging from the host. We found that parasitism mainly occurred in termite mounds overgrown with grass and mounds that had been broken up previously for other experiments. The parasitized soldiers showed a significantly lower level of interspecific aggressiveness compared with healthy soldiers (P < 0.05). Parasitized soldiers also changed in habitat preference to one isolated chamber of the nest. This might be an adaptive strategy that facilitates parasitoid dispersal, provides protection to parasitoids, and reduces the risk of parasitism to host colony. An abnormally rounded head capsule and remarkably short mandibles are characteristics of a parasitized soldier. The older larval fly stages were found only in major soldiers. We suggest that parasitization may first start in fourth or even earlier larval termite instars. The fly larva develops in the termite soldier's head capsule and pupates inside the host's body.
    Matched MeSH terms: Larva/physiology
  5. Mukai Y, Chai LL, Shaleh SR, Senoo S
    Zoolog Sci, 2007 Aug;24(8):829-35.
    PMID: 18217491
    This study was conducted to clarify the development of free neuromasts with growth of the barramundi, Lates calcarifer. A pair of free neuromasts was observed behind the unpigmented eyes in newly hatched eleutheroembryos with a mean total length of 1.93 mm, and two-hour-old eleuthero-embryos could respond to an approaching pipette. At 2 days after hatching, the egg yolk sac was mostly consumed, the eyes were pigmented, and the larvae commenced feeding on rotifers. Free neuromasts increased in number with growth and commenced developing into canal neuromasts in barramundi 15 days old with a mean total length of 8.07 mm. The average length of the major axis of the trunk free neuromasts attained approximately 12.9-15.5 microm, and the number of sensory cells was 15.4-17.5 at 15-20 days old. Developed cupulae of free neuromasts were observed in 1-day-old eleutheroembryos. The direction of maximum sensitivity of free neuromasts, determined from the polarity of the sensory cells, coincided with the minor axis of the lozenge-shaped outline of the apical surface of the free neuromasts. The polarity of trunk neuromasts was usually oriented along the antero-posterior axis of the fish body, but a few had a dorso-ventral direction. On the head, free neuromasts were oriented on lines tangential to concentric circles around the eye.
    Matched MeSH terms: Larva/physiology*
  6. Ara R, Arshad A, Amin SMN, Idris MH, Gaffar MA, Romano N
    J Environ Biol, 2016 07;37(4 Spec No):745-54.
    PMID: 28779734
    Our previous study demonstrated that among different habitat sites (mangrove, estuary, river, seagrass and Open Sea) in Johor Strait, Malaysia, seagrass showed highest family diversity and abundance of larval fish. However, it is unclear whether this was due to difference in habitat complexity or water quality parameters.? To test this, larval fish were collected by using a bongo net equipped with a flow meter by subsurface horizontal towing from different habitats in Johor Strait between October 2007 and September 2008.? Various physico-chemical parameters were measured and then examined for any relationship to fish larvae diversity and abundance. Among the 24 families identified from the sites, seven families (Blenniidae, Clupeidae, Mullidae, Nemipteridae, Syngnathidae, Terapontidae and Uranoscopeidae) were significantly correlated with the tested waters quality parameters.? Salinity showed a positive and negative significant correlation with Clupeidae (p < 0.01) and Uranoscopeidae (p < 0.05), respectively. Terapontidae was significantly correlated with dissolved oxygen (p < 0.01), while both Mullidae and Syngnathidae were significantly correlated with pH (p < 0.05). However, a canonical correspondence analysis test indicated weak overall correlation (36.4%) between larval assemblage and in the seagrass-mangrove ecosystem of Johor Strait, Malaysia. This likely indicates that habitat structure was more important in determining larval abundance (highest in the seagrass habitat) as compared to water quality at the tested sites. This study emphasizes the need to conserve seagrass beds as important nursery grounds for various fish larvae to ensure adequate recruitment and ultimately sustainable fisheries management. ?
    Matched MeSH terms: Larva/physiology
  7. Srisuka W, Takaoka H, Otsuka Y, Fukuda M, Thongsahuan S, Taai K, et al.
    Parasit Vectors, 2017 Nov 21;10(1):574.
    PMID: 29157269 DOI: 10.1186/s13071-017-2492-y
    BACKGROUND: Blackflies are an important medical and veterinary group of small blood-sucking insects. Ninety-three blackfly species have been reported in Thailand. However, information on their biodiversity and population dynamics in each region is lacking. The main aim of this study was to assess the regional biodiversity, seasonal abundance and distribution of blackflies in six eco-geographically different regions in the country.

    METHODS: Blackfly larvae and pupae were sampled monthly from 58 sites between May 2011 and April 2013. Diversity parameters, seasonal abundance, regional distribution and frequency of species occurrence in stream sites were analyzed.

    RESULTS: A total of 19,456 mature larvae representing 57 species, and belonging to six subgenera in the genus Simulium Latreille (s.l.), were found. The five predominant taxa were S. fenestratum (8.6%), the S. asakoae complex (8.3%), S. nakhonense (7.5%), the S. siamense complex (7.4%) and the S. doipuiense complex (6.7%). The most frequent taxa at all sites were the S. asakoae complex (84.5%), followed by S. fenestratum (82.8%), the S. siamense complex (75.9%), S. decuplum (60.3%), S. nakhonense (58.6%) and the S. tani complex (48.3%). The richness of regional species was highest (40 species) in the north and predominated in the cold season. However, blackflies in the south predominated during the hot season. The highest numbers of blackflies collected from central, northeastern, eastern and western regions of the country were observed in the rainy season. Overall, the mean number of blackflies collected across the six regions during the rainy and cold season had no statistically significant difference, but it differed significantly in the hot season.

    CONCLUSIONS: Blackflies in Thailand were surveyed in all three seasons across six geographical regions. These findings demonstrated that blackfly communities at each stream site varied with seasonality, and the regional relative abundance of blackflies differed markedly in the hot season. It was also found that the occurrence and distribution of blackflies in each region were associated strongly with elevation.

    Matched MeSH terms: Larva/physiology
  8. Ahmad R, Suzilah I, Wan Najdah WMA, Topek O, Mustafakamal I, Lee HL
    PLoS One, 2018;13(2):e0193326.
    PMID: 29474401 DOI: 10.1371/journal.pone.0193326
    A large scale study was conducted to elucidate the true relationship among entomological, epidemiological and environmental factors that contributed to dengue outbreak in Malaysia. Two large areas (Selayang and Bandar Baru Bangi) were selected in this study based on five consecutive years of high dengue cases. Entomological data were collected using ovitraps where the number of larvae was used to reflect Aedes mosquito population size; followed by RT-PCR screening to detect and serotype dengue virus in mosquitoes. Notified cases, date of disease onset, and number and type of the interventions were used as epidemiological endpoint, while rainfall, temperature, relative humidity and air pollution index (API) were indicators for environmental data. The field study was conducted during 81 weeks of data collection. Correlation and Autoregressive Distributed Lag Model were used to determine the relationship. The study showed that, notified cases were indirectly related with the environmental data, but shifted one week, i.e. last 3 weeks positive PCR; last 4 weeks rainfall; last 3 weeks maximum relative humidity; last 3 weeks minimum and maximum temperature; and last 4 weeks air pollution index (API), respectively. Notified cases were also related with next week intervention, while conventional intervention only happened 4 weeks after larvae were found, indicating ample time for dengue transmission. Based on a significant relationship among the three factors (epidemiological, entomological and environmental), estimated Autoregressive Distributed Lag (ADL) model for both locations produced high accuracy 84.9% for Selayang and 84.1% for Bandar Baru Bangi in predicting the actual notified cases. Hence, such model can be used in forestalling dengue outbreak and acts as an early warning system. The existence of relationships among the entomological, epidemiological and environmental factors can be used to build an early warning system for the prediction of dengue outbreak so that preventive interventions can be taken early to avert the outbreaks.
    Matched MeSH terms: Larva/physiology
  9. Saifur RG, Dieng H, Hassan AA, Satho T, Miake F, Boots M, et al.
    J Am Mosq Control Assoc, 2010 Dec;26(4):373-80.
    PMID: 21290932
    Moisture plays a major role in the dynamics of mosquito populations, especially those breeding in container habitats. Despite this importance, the role of moisture conditions as they affect oviposition and egg development in Aedes vectors remains largely unexplored. We investigated the effect of exposing gravid female Aedes albopictus mosquitoes and their eggs to different moisture levels (MLs) for various periods on oviposition and hatching. Overall, high-moisture substrates (HMSs; 66% and 72%) provided better environments for egg laying. The timing of initial egg laying was far longer at the lowest substrate moisture level (LSML, 25% and 41.2%) than at HMSs. The numbers of eggs laid were much lower in the drier environments. At LSMLs, gravid females retained increasing numbers of mature eggs until death, and egg retention decreased gradually with increasing ML. The HMSs also provided better environments for larval eclosion. The numbers of eggs hatched were lower at the LSML than the HSML environment. No egg hatching occurred after 1 h exposure to moisture. However, egg hatching occurred by installment, with spontaneous hatching (SH) increasing gradually with increasing ML. High-moisture conditions combined with long exposure (30 h and 48 h) favored SH. These results suggest that Ae. albopictus females can respond to better moisture conditions for increased success of embryonation and larval eclosion. This information may be useful in the colonization of floodwater Aedes species.
    Matched MeSH terms: Larva/physiology
  10. Kassim NF, Webb CE, Russell RC
    J Am Mosq Control Assoc, 2012 Dec;28(4):312-6.
    PMID: 23393755
    Culex molestus is an obligatory autogenous mosquito that is closely associated with subterranean habitats in urban areas. The objective of our study was to investigate the influence of larval and adult nutrition on the role of males in determining the expression of autogeny in Cx. molestus. Mosquitoes raised at low and high larval diets had sex ratio, wing length, mating rates, autogenous egg raft size, and hatching rates recorded. There was a higher ratio of males to females when raised at a low larval diet. Mean wing lengths of both males and females were significantly greater when raised at the high larval diet regime. Regardless of larval or adult diet, males mated with only a single female. Mosquitoes raised at the higher larval diet regimes developed significantly more autogenous eggs. However, the egg raft size was reduced when adult females were denied access to sugar. The results of this study indicate that the performance of males in the reproductive process is influenced by both larval diet and adult sugar feeding.
    Matched MeSH terms: Larva/physiology
  11. Neoh KB, Lee CY
    J Insect Sci, 2011;11:47.
    PMID: 21861651 DOI: 10.1673/031.011.4701
    The larval parasitoid Verticia fasciventris Malloch (Diptera: Calliphoridae) develops in the head of soldiers of the fungus-growing termite Macrotermes carbonarius (Hagen) (Isoptera: Termitidae). Morphological and behavioral changes in the host were evaluated and the termite castes and stages that were parasitized were identified. The larval emergence process is also described and possible mechanisms for the parasitoid fly's entry into the host body are discussed based on qualitative observations. Only a single larva per host was found. The mature larva pupated outside the host's body by exiting between the abdominal cerci. Parasitized soldiers possess a short and square-shaped head capsule, a pair of notably short mandibles, and a pair of 18-segmented antennae. Although parasitized soldiers were statistically less aggressive than healthy soldiers (P < 0.05), they expressed varying levels of aggression. Both minor and major soldiers can be parasitized and based on evidence from presoldiers, parasitization may begin during the precursor stages of soldiers. However, the stage at which parasitism first occurs has not been determined.
    Matched MeSH terms: Larva/physiology
  12. Ahmad Firdaus MS, Marwi MA, Syamsa RA, Zuha RM, Ikhwan Z, Omar B
    Trop Biomed, 2010 Apr;27(1):134-7.
    PMID: 20562824
    Hypopygiopsis violacea, a species of fly of forensic importance, was recovered from a corpse and described for the first time. The morphological structures of the second and third instar larvae of four specimens were examined using light microscope. Observations were focused on three main morphological characters: cephalopharyngeal skeleton, anterior and posterior spiracles. Cephalopharyngeal skeleton of second instar larva is darkly pigmented and without accessory sclerite below the mouth hook. The anterior spiracles of second and third instar larvae have 8-9 papillae each, arranged in a single row. The posterior spiracle of second instar larva has two spiracular slits with no thickening of peritreme. This differentiates it from the third instar, whereby the latter has three slits for each posterior spiracle. Cephalopharyngeal skeleton of third instar larva is heavily pigmented. An accessory sclerite is found below the hook part of third instar larva but is absent in second instar. Peritreme of the posterior spiracle of third instar larva is thick almost complete encircling a button. The intersegmental spines of the cuticular surface are dome-shaped and unicuspid. Third instar larva of this species is large with size approximately 15 mm long. These findings provide important identification features of immature stages of Hy. violacea which could be useful in forensic entomology.
    Matched MeSH terms: Larva/physiology
  13. Foo FK, Singham GV, Othman AS, Lee CY
    J Econ Entomol, 2011 Oct;104(5):1675-9.
    PMID: 22066198
    A survey of the infestation rate of colonies of Macrotermes gilvus (Hagen) (Termitidae: Macrotermitinae) with the koinobiont endoparasitoid Misotermes mindeni Disney & Neoh (Diptera: Phoridae) was conducted in Malaysia from September 2009 to January 2011 in the states of Kedah, Penang, Perak, Selangor, Kuala Lumpur, Johor, Terengganu, and Sarawak. Of the 1,125 M. gilvus mounds surveyed, 12.4% contained termites parasitized by M. mindeni and these mounds occurred only in the states of Penang and Perak. High frequencies of mounds containing parasitized termites were found at sites in Penang: Bayan Lepas (21.1%), Minden Campus of Universiti Sains Malaysia ([USM]; 24.5%), Teluk Bahang (28.0%), and Bukit Mertajam (35.0%); the lowest frequency (4.0%) was recorded from Gelugor. The parasitized colonies at all sites were classified as healthy, with exception of several from the Minden Campus of USM (96.4% healthy) and Ayer Itam (87.5% healthy). Most parasitized colonies (71.2%) had a low level of M. mindeni infestation. Only 16.7 and 12.1% of the infested colonies had moderate or high parasite infestation levels, respectively. The height of infected mounds was significantly higher than that of the healthy mounds, but there was no difference between the mound diameters of infested and uninfested mounds. Parasite infestation level was not significantly correlated with mound height or mound diameter. The ambient light intensity at sites with infested mounds was significantly lower than that of uninfested mounds. There was also a significant negative relationship between light intensity and degree of parasitism.
    Matched MeSH terms: Larva/physiology
  14. Armstrong JW, Tang J, Wang S
    J Econ Entomol, 2009 Apr;102(2):522-32.
    PMID: 19449631
    The late-aged egg and third-instar life stages of laboratory-reared Malaysian fruit fly, Bactrocera latifrons (Hendel); Mediterranean fruit fly, Ceratitis capitata (Wiedemann); melon fly, B. cucurbitae Coquillett; and oriental fruit fly, B. dorsalis (Hendel), (Diptera: Tephritidae); and the third instars of wild Mediterranean fruit fly were exposed to thermal treatments. A heating block system was used to determine the thermal death kinetics of the four fruit fly species. Treatments consisted of heating the fruit fly life stages to 44, 46, 48, and 50 degrees C and holding for different times ranging from 0 to 120 min depending on the thermal mortality response and time required to obtain 100% mortality for each species and life stage. The 0.5-order kinetic model had the best fit to the survival ratio for all the treatment temperatures and was used to predict lethal times. The thermal death time (TDT) curves showed a tolerance order of Mediterranean fruit fly eggs < or = third instars at 44, 46, and 50 degrees C, third instars < or = eggs at 48 degrees C, and wild third instars < the laboratory-reared third instars. Comparison between Mediterranean fruit fly third instar thermotolerance from Hawaii and Israel showed that Israel Mediterranean fruit fly was more thermotolerant. A comparison of minimum treatment times at a given temperature required to obtain 100% mortality of laboratory-reared Malaysian, Mediterranean (Hawaii and Israel strains), melon, Mexican, and oriental fruit fly eggs or third instars and wild Mediterranean fruit fly (Hawaii strain) eggs or third instars showed that oriental fruit fly was the most thermotolerant among the third instars, and the difference in heat tolerance between third instars and eggs was negligible at 50 degrees C.
    Matched MeSH terms: Larva/physiology
  15. Ivorra T, García-Martínez B, Martínez-Sánchez A
    J Med Entomol, 2021 11 09;58(6):2247-2254.
    PMID: 34279664 DOI: 10.1093/jme/tjab129
    To better understand the population dynamics and dispersal ability of insect species, it is often helpful to derive a life table containing fundamental demographic data. The aim of this study was to determine a life table for the predatory necrophagous species Synthesiomyia nudiseta (van der Wulp, 1883) on a pig liver diet and under controlled laboratory conditions (29.5 ± 2. 5°C, RH 50 ± 15%, and a photoperiod of 12:12). This species has medical and veterinary importance and its distribution extends in tropical and subtropical areas and now it has been established in the southwestern of Europe. The mean adult longevity was 36. 18 ± 2. 06 d and the net reproduction rate, R, was 27.65 offspring/female, the mean generation time, T, was 22. 09 d, the finite rate of increase, λ, was 1. 16 d-1, and the intrinsic rate of increase, r, was 0. 15 d-1. These results indicate that S. nudiseta cannot be considered an r-strategist as the most common synanthropic necrophagous blowflies due to its predatory behavior; however, its invasive and colonist abilities are discussed. This is the first life table study of this species from Palearctic region to analyze the effect of its dispersal ability.
    Matched MeSH terms: Larva/physiology
  16. Kermani N, Abu Hassan ZA, Suhaimi A, Abuzid I, Ismail NF, Attia M, et al.
    PLoS One, 2014;9(6):e100671.
    PMID: 24968125 DOI: 10.1371/journal.pone.0100671
    The diamondback moth (DBM) Plutella xylostella (L.) has traditionally been managed using synthetic insecticides. However, the increasing resistance of DBM to insecticides offers an impetus to practice integrated pest management (IPM) strategies by exploiting its natural enemies such as pathogens, parasitoids, and predators. Nevertheless, the interactions between pathogens and parasitoids and/or predators might affect the effectiveness of the parasitoids in regulating the host population. Thus, the parasitism rate of Nosema-infected DBM by Cotesia vestalis (Haliday) (Hym., Braconidae) can be negatively influenced by such interactions. In this study, we investigated the effects of Nosema infection in DBM on the parasitism performance of C. vestalis. The results of no-choice test showed that C. vestalis had a higher parasitism rate on non-infected host larvae than on Nosema-treated host larvae. The C. vestalis individuals that emerged from Nosema-infected DBM (F1) and their progeny (F2) had smaller pupae, a decreased rate of emergence, lowered fecundity, and a prolonged development period compared to those of the control group. DBM infection by Nosema sp. also negatively affected the morphometrics of C. vestalis. The eggs of female C. vestalis that developed in Nosema-infected DBM were larger than those of females that developed in non-infected DBM. These detrimental effects on the F1 and F2 generations of C. vestalis might severely impact the effectiveness of combining pathogens and parasitoids as parts of an IPM strategy for DBM control.
    Matched MeSH terms: Larva/physiology
  17. Burg NA, Pradhan A, Gonzalez RM, Morban EZ, Zhen EW, Sakchoowong W, et al.
    PLoS One, 2014;9(8):e104076.
    PMID: 25119899 DOI: 10.1371/journal.pone.0104076
    The Neotropical butterfly Dryas iulia has been collected from several locations in Thailand and Malaysia since 2007, and has been observed breeding in the wild, using introduced Passiflora foetida as a larval host plant. The butterfly is bred by a butterfly house in Phuket, Thailand, for release at weddings and Buddhist ceremonies, and we hypothesized that this butterfly house was the source of wild, Thai individuals. We compared wing patterns and COI barcodes from two, wild Thai populations with individuals obtained from this butterfly house. All Thai individuals resemble the subspecies D. iulia modesta, and barcodes from wild and captive Thai specimens were identical. This unique, Thai barcode was not found in any of the 30 specimens sampled from the wild in the species' native range, but is most similar to specimens from Costa Rica, where many exporting butterfly farms are located. These data implicate the butterfly house as the source of Thailand's wild D. iulia populations, which are currently so widespread that eradication efforts are unlikely to be successful.
    Matched MeSH terms: Larva/physiology
  18. Takaoka H, Sofian-Azirun M, Ya'cob Z, Hashim R
    Zootaxa, 2014;3765:54-68.
    PMID: 24870884 DOI: 10.11646/zootaxa.3765.1.3
    Two new black fly species, Simulium (Gomphostilbia) brinchangense and S. (G.) tanahrataense, are described on the basis of reared adult females, males, pupae and larvae from Cameron's Highlands, Peninsular Malaysia. These new species are assigned to the asakoae species-group within Simulium (Gomphostilbia) and taxonomic notes are given to distinguish each new species from six known species in Malaysia. Revised keys to identify all 21 species including 13 species from other countries are provided for females, males, pupae and mature larvae. The species diversity of the asakoae species-group in Cameron's Highlands is briefly noted.
    Matched MeSH terms: Larva/physiology
  19. Zuha RM, Omar B
    Parasitol Res, 2014 Jun;113(6):2285-94.
    PMID: 24728523 DOI: 10.1007/s00436-014-3883-z
    Cosmopolitan scuttle fly, Megaselia scalaris (Loew) (Diptera: Phoridae) is one of the commonest forensic species recorded colonizing human corpse indoors and in concealed environment. The occurrence of this species in such environments provides a higher evidential value to assist estimation of postmortem interval (PMI) compared to other forensically important dipterans. However, developmental and size data of M. scalaris are still lacking and they are derived from a limited range of thermal values. The objective of this study is to develop the growth model of M. scalaris by emphasizing the size range of larvae and puparia at different constant temperatures. This species was reared in six replicates at eight varying constant temperatures ranging from 23 to 36 °C and cow's liver was provided as food source. Larvae and puparia were sampled at set time intervals and measured by their length and weight. Because interpretation of forensic entomological evidence is subject to application of different techniques, development of M. scalaris is expressed herein by using developmental table, length/morphological stage diagrams and linear/nonlinear estimation methods. From the findings, it is very important to highlight that sexual dimorphism of M. scalaris during post feeding larva and pupa stage could be observed based on size and developmental periods. Mean length and weight ratios of male to female puparia are approximately 0.8 and 0.3-0.5, respectively, indicating sexual dimorphism of this species. Developmental period in female are 4.0-11.4 h (post feeding larval stage), 3.7-24.0 h (pupal stage), and 3.0-20.1 h (total developmental period) longer in male. Due to this dimorphism, PMI estimation using M. scalaris post feeding larva or puparium specimens must be carried out carefully by to avoid inaccuracy and misinterpretation.
    Matched MeSH terms: Larva/physiology
  20. Abu Tahir N, Ahmad AH
    J Med Entomol, 2013 Sep;50(5):999-1002.
    PMID: 24180104
    Effects of laterite cover soil with different characteristics on survival of buried eggs, third instar larvae, and pupae of Musca domestica (L.) were studied experimentally. Soil treatments were loose dry soil, loose wet soil, compacted dry soil, and compacted wet soil (CWS). Eggs, third instar larvae, and pupae were buried under 30 cm of the different soil treatments and placed under field conditions until adults emerged. Rearing medium was provided for eggs and larvae, and control treatments of all stages were unburied immatures placed on soil surface. Egg and pupal survival to adult were significantly affected by the cover soil treatments, but third instars were more resilient. Wet soil treatments (loose wet soil and CWS) resulted in significantly reduced pupal survival, but increased survival of eggs. However, CWS significantly reduced adult emergence from buried eggs. Though emergence of house flies buried as eggs was significantly reduced, some were able to hatch and emerging first instar larvae developed to pupation. Although cover soil does not completely prevent fly emergence, it did limit development and emergence of buried house flies.
    Matched MeSH terms: Larva/physiology
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