Browse publications by year: 2003

  1. Wan Nudri WD, Mohamed Rusli A, Wan Abdul Manan W, Mohd Rafi M, Naing L, Kamarul Imran M, et al.
    Malays J Nutr, 2003 Sep;9(2):95-103.
    PMID: 22691731
    A cross-sectional comparative study was conducted to determine the nutritional status among physically active groups in Kota Bharu. The study population comprised 83 adult male athletes from 8 different types of sports (athlete group), 80 active men who exercised a minimum of 30 min per day for at least 3 times per week (exercise group), and 80 inactive men (sedentary group). All the respondents were aged between 18 to 44 years. Measurements taken from the respondents were anthropometric measurements, systolic (SBP) and diastolic (DBP) blood pressure, and serum total cholesterol (TC). The results showed that the combined prevalence of pre-obese (BMI 25.0-29.9) and obese (BMI ≥30.0) was 21.7% in athletes, 29.9% in exercise group, and 47.5% in sedentary group. The mean (± SD) percentage of body fat in athletes was 15.7 ± 5.4%, which was lower compared to the exercise (18.9 ± 5.5%) and sedentary (20.6 ± 5.8%) groups. The incidence of waist-to-hip ratio above 0.9 in athlete, exercise and sedentary groups was 9.6%, 18.7% and 31.3%, respectively. The incidence of hypertension (SBP ≥140 and/or DBP ≥90 mmHg) was 13.2% in athletes, 17.5% in exercise group and 42.5% in the sedentary group. The TC values showed that the prevalence of "high risk" individuals (TC ≥6.20 mmol/l) was also lower in athletes (20.5%), compared to the exercise (36.2%) and sedentary (47.5%) groups. The study revealed that individuals who are actively involved in physical activity, particularly in sport activities have better nutritional status compared to sedentary people. However, the nutritional status in the athlete and exercise groups was still unsatisfactory. The incidence of poor health status related to over nutrition in the active groups was rather high and needs attention from health professionals. Further studies are needed to determine nutritional practices among physically active groups.
  2. Lim HM, Chee H, Kandiah M, Shamsuddin K, Jamaluddin J, Nordin N, et al.
    Malays J Nutr, 2003 Sep;9(2):105-24.
    PMID: 22691732 MyJurnal
    This study was a cross-sectional survey conducted among 122 women workers employed in the electronics factories in the Ulu Klang Free Trade Zone (FTZ) and the Bangi FTZ, Selangor, Peninsular Malaysia. The purpose of the study was to examine the problem of overweight (>25.0 kg/m2) among this group of women, and factors (socio-demographic, work, exercise, and dietary) associated with overweight, and, to study the food intake pattern of the women in both the overweight and non-overweight groups. Data was collected using a set of questionnaires, while anthropometric measurements were obtained to calculate body mass index (BMI) and waist hip ratio (WHR). The results of the study indicated that 64.0% of the women were overweight (29.5% pre-obese, 34.5% obese). About one-tenth of the women (11.5%) had a WHR of above 0.85. From the bivariate analysis, it was found that women who were older, ever married, had lower educational level, had higher salary, not living in the hostel, involved in shiftwork, and trying to lose weight were more likely to be overweight. After adjusting for age, each of the above factors, except for educational level, remained significantly associated with overweight. Women's diet was found to be monotonous and lacking in variety as accessibility to and availability of a variety of food was a problem for them due to the nature of their work. They also had a sedentary lifestyle. Therefore, further research focusing on changing the poor dietary habits and sedentary lifestyle of the women workers is necessary to address the problem of overweight.
  3. Karim NA, Mydenkather H
    Malays J Nutr, 2003 Sep;9(2):125-36.
    PMID: 22691733 MyJurnal
    A food habits and health status study was carried out among 100 Malay adults aged 40 years and above. The study protocol incorporated anthropometric measurements, evaluation of food habits and determination of blood glucose, cholesterol and blood pressure. Mean body mass index (BMI) for men and women were 27.2 ± 4.2 kg/m2 and 27.0 ± 5.2 kg/m2 respectively, which on average showed that the subjects were overweight. Food habits evaluation indicated that rice, fish and vegetables were the foods consumed almost everyday by the majority of the subjects. Meat, dairy products and fruits were eaten once to three times per week. Food intake score for sugar and salt demonstrated that a majority of men and women consumed moderate amounts of these foods. Most subjects exercised twice to three times a week for 15 min per session. Blood glucose tests revealed a mean of 5.04 ± 1.60 mmol/l in men and 4.86 ± 2.10 mmol/l for women. Mean cholesterol for men was 5.06 ± 1.22 mmol/l while it was 4.90 ± 1.34 mmol/l in women. Mean systolic and diastolic blood pressure in men was normal of 127.69 ± 13.60 mm Hg dan 85.87 ± 7.97 mm Hg, while in women it was 127.42 ± 17.54 mm Hg, 83.53 ± 9.50 mm Hg. The mean value for glucose, cholesterol and blood pressure increased with age. The food habits of these adults were satisfactory; however blood test for the nutrients of interest exhibited an increasing trend towards blood pressure, blood cholesterol and glucose with age. Middle-aged adults should adapt to a more active lifestyle and be more cautious of their food habits. This is to ensure a healthy well being throughout their life span.
  4. Mohd Sidik S, Ahmad R
    Malays J Nutr, 2003 Sep;9(2):137-44.
    PMID: 22691734
    The Ministry of Health has launched healthy lifestyle and diabetes campaigns. However, adopting healthy lifestyle practices is not easy as revealed in this case. Patients and their families need to be supervised personally and counseled regularly. Healthy lifestyle habits such as maintaining a balanced diet, ideal body weight and physical activities need to be cultivated and practised. Dietary counseling sessions need to be implemented, preferably by dietitians as this has been shown to be effective in motivating diabetic patients to achieve a better food choice, as well as better glycaemic, lipid and weight control, as shown in this case.
  5. Goh AY, Chan TL, Abdel-Latiff ME
    Acta Paediatr, 2003 Aug;92(8):965-9.
    PMID: 12948074
    AIM: Knowledge of the spectrum and frequencies of pediatric emergencies presenting to an emergency department (ED) of individual developing countries is vital in optimizing the quality of care delivered locally.

    METHODS: A prospective 6 wk review of all pediatric (< 18 y) attendees to an urban ED was done, with patient age, presenting complaints, diagnoses, time of arrival and disposition recorded.

    RESULTS: Complete data were available on 1172 patients, with an age range of 4 d to 18 y (mean +/- SD 6.9 +/- 5.6 y); 43% were aged < or = 4 y. The main presenting complaints were injuries (26.9%), fever (24%) and breathing difficulties (16.6%). The most common diagnosis was minor trauma (24.2%), with soft-tissue injuries predominating (80.6%). The other diagnoses were asthma (12.6%), upper respiratory infections (12.1%), other infections (12.1%) and gastroenteritis (11.8%). Equal proportions of patients were seen throughout the day. 25% of patients were admitted. Young age (< 1 y); presence of past medical history, general practitioner referrals, diagnosis of bronchiolitis and pneumonia were significantly associated with risk of admission.

    CONCLUSION: A wide spectrum of paediatric illnesses was seen in the ED, with an overrepresentation of young children. This supports the decision to have either a separate pediatric ED or paediatric residents on the staff. The training curricula should emphasize the management of pediatric trauma, infections and asthma. Alternatively, developing guidelines for the five most common presenting complaints would account for 82% of all attendees and could be directed towards all staff on the ED.

    MeSH terms: Adolescent; Age Factors; Child; Child, Preschool; Developing Countries*; Emergency Service, Hospital/statistics & numerical data*; Humans; Infant; Malaysia; Pediatrics*; Prospective Studies
  6. Ang HH, Lee EL, Matsumoto K
    Hum Exp Toxicol, 2003 Aug;22(8):445-51.
    PMID: 12948085 DOI: 10.1191/0960327103ht382oa
    In Malaysia, the phase 3 registration for traditional medicines was implemented on 1 January 1992 under the Control of Drugs and Cosmetics Regulation 1984, emphasizing quality, efficacy and safety (including the detection of the presence of heavy metals) in all pharmaceutical dosage forms of traditional medicine preparations. Therefore, a total of 100 products in various pharmaceutical dosage forms of a herbal preparation, were analysed for lead content using atomic absorption spectrophotometer. Results showed that 8% (eight products) possessed 10.64-20.72 ppm of lead, and therefore, do not comply with the quality requirement for traditional medicines in Malaysia. One of these products, M-Tongkat Ali (exhibited 10.64 +/-0.37 ppm of lead), was in fact already registered with the DCA Malaysia. The rest, Sukarno Tongkat Ali, Eurycoma Madu, Super Pill Tongkat Ali, Force Pill Tongkat Ali, Tender Pill Tongkat Ali, Super Pill Tongkat Ali Plus and Great Pill Tongkat Ali Plus have not registered with the DCA Malaysia and exhibited 12.24-20.72 ppm of lead. Although this study showed that only 92% of the products complied with the quality requirement for traditional medicines in Malaysia, however, they cannot be assumed safe from lead contamination because of batch-to-batch inconsistency.
    MeSH terms: Capsules; Drug Combinations; Lead/analysis*; Lead Poisoning/etiology; Lead Poisoning/epidemiology; Malaysia/epidemiology; Plants, Medicinal/chemistry*; Spectrophotometry, Atomic; Tablets; Plant Preparations/analysis*; Plant Preparations/poisoning
  7. Liu W, Zhang J, Hashim JH, Jalaludin J, Hashim Z, Goldstein BD
    Environ Health Perspect, 2003 Sep;111(12):1454-60.
    PMID: 12948883
    Burning mosquito coils indoors generates smoke that can control mosquitoes effectively. This practice is currently used in numerous households in Asia, Africa, and South America. However, the smoke may contain pollutants of health concern. We conducted the present study to characterize the emissions from four common brands of mosquito coils from China and two common brands from Malaysia. We used mass balance equations to determine emission rates of fine particles (particulate matter < 2.5 microm in diameter; PM(2.5)), polycyclic aromatic hydrocarbons (PAHs), aldehydes, and ketones. Having applied these measured emission rates to predict indoor concentrations under realistic room conditions, we found that pollutant concentrations resulting from burning mosquito coils could substantially exceed health-based air quality standards or guidelines. Under the same combustion conditions, the tested Malaysian mosquito coils generated more measured pollutants than did the tested Chinese mosquito coils. We also identified a large suite of volatile organic compounds, including carcinogens and suspected carcinogens, in the coil smoke. In a set of experiments conducted in a room, we examined the size distribution of particulate matter contained in the coil smoke and found that the particles were ultrafine and fine. The findings from the present study suggest that exposure to the smoke of mosquito coils similar to the tested ones can pose significant acute and chronic health risks. For example, burning one mosquito coil would release the same amount of PM(2.5) mass as burning 75-137 cigarettes. The emission of formaldehyde from burning one coil can be as high as that released from burning 51 cigarettes.
    MeSH terms: Animals; Carcinogens/analysis*; Fixatives/analysis; Formaldehyde/analysis; Humans; Insecticides/analysis*; Malaysia; Mosquito Control*; Particle Size; Public Health*; Volatilization; Air Pollution, Indoor/analysis*; Incineration; Risk Assessment
  8. Nakajima Y, Ho CC, Kudo T
    J Gen Appl Microbiol, 2003 Jun;49(3):181-9.
    PMID: 12949699
    The taxonomic position of three actinomycete strains isolated from Malaysian soil was established by using a polyphasic approach. The isolates formed chains composed of four spores on the tip of sporophores branching from the aerial mycelium, and their chemotaxonomic properties were common to those of members of the family Streptosporangiaceae. These phenotypic properties as well as a phylogenetic analysis based on 16S rRNA gene sequences indicated that they should be classified in the genus Microtetraspora. The three isolates showed a unique pattern of cultural, physiological and biochemical properties that distinguished them from previously described species of the genus Microtetraspora. The isolates showed more than 72% DNA relatedness to each other, but only 58% or less relatedness to any previously described species. On the basis of the data presented, a new species of the genus Microtetraspora, Microtetraspora malaysiensis, is proposed. The type strain of the new species is strain H47-7(T) (=JCM 11278(T)=DSM 44579(T)).
    MeSH terms: Base Composition; Base Sequence; DNA, Bacterial/genetics; DNA, Bacterial/chemistry; Malaysia; Microscopy, Electron, Scanning; Molecular Sequence Data; Nucleic Acid Hybridization; RNA, Ribosomal, 16S/genetics; RNA, Ribosomal, 16S/chemistry; Soil Microbiology*; Polymerase Chain Reaction; Actinobacteria/genetics; Actinobacteria/isolation & purification*; Actinobacteria/metabolism; Actinobacteria/ultrastructure
  9. Kim LH, Peh SC, Poppema S
    Int J Cancer, 2003 Nov 1;107(2):250-5.
    PMID: 12949802
    Isolation of single cells permits analysis of DNA or RNA from individual cells among heterogeneous populations. This technique is particularly useful in the study of classical Hodgkin's lymphoma (cHL) due to the scarcity of H/RS tumor cells among large numbers of reactive leukocytes. In a previous study, we found a high frequency of dual LMP-1 variant (concurrent presence of deleted and nondeleted variants) in cHL from whole-tissue sections. For the present study, we applied a single-cell isolation technique to determine the LMP-1 oncogene variant in EBV-associated H/RS cells. Five cases of EBV-infected cHL, containing nondeleted (n=1), deleted (n=1) and dual infection (n=3) based on whole-tissue section analysis, were selected for study. Paraffin-embedded tissue sections were stained with antibody to LMP-1 and positively stained H/RS cells isolated using a semiautomated micromanipulator. Each isolated single cell was subjected to PCR for amplification of the LMP-1 gene flanking the 30 bp deletion region and Xho1 restriction site. Cases with either nondeleted variant or the deleted variant showed similar LMP-1 variant expression in isolated single H/RS cells. However, 1 of the 3 cases with dual variants showed only the deleted variant in H/RS cells. The other 2 cases showed mixed patterns of deleted, nondeleted and dual LMP-1 variants in isolated single H/RS cells. All cases showed loss of the Xho1 restriction site, with the exception of the case with nondeleted LMP-1. Results of single-H/RS cell analysis of the Xho1 restriction site concur with those of whole-tissue section amplification. A mixed pattern of LMP-1 variants was observed in isolated H/RS cells, and it is speculated that this is due to the accumulation of mutation and deletion events.
    MeSH terms: Base Sequence; DNA, Viral/analysis; Herpesvirus 4, Human/genetics; Herpesvirus 4, Human/isolation & purification*; Hodgkin Disease/pathology; Hodgkin Disease/virology*; Humans; Immunoenzyme Techniques; Molecular Sequence Data; Tumor Virus Infections/virology; Genetic Variation; Viral Matrix Proteins/genetics*; Viral Matrix Proteins/metabolism; Blotting, Southern; Restriction Mapping; Immunophenotyping; Polymerase Chain Reaction/methods; Reed-Sternberg Cells/pathology; Reed-Sternberg Cells/virology*; Paraffin Embedding; Gene Deletion; Epstein-Barr Virus Infections/virology
  10. Peh SC, Shaminie J, Poppema S, Kim LH
    Singapore Med J, 2003 Apr;44(4):185-91.
    PMID: 12952030
    Castleman's disease is an uncommon disease and the histopathogenesis is poorly understood. This study aims to investigate their clinicopathological and immunophenotypic profile.
    MeSH terms: Adolescent; Adult; Child; DNA-Binding Proteins/metabolism; Female; Giant Lymph Node Hyperplasia/metabolism; Giant Lymph Node Hyperplasia/pathology*; Humans; Hyperplasia; Immunohistochemistry; Lymph Nodes/pathology; Lymphocytes/metabolism; Lymphocytes/pathology; Male; Middle Aged; Proto-Oncogene Proteins/metabolism; Palatine Tonsil/pathology; Transcription Factors/metabolism; Antigens, CD/metabolism; Gene Expression; Immunophenotyping*; In Situ Hybridization; Proto-Oncogene Proteins c-bcl-6
  11. Nissapatorn V, Lee CK, Khairul AA
    Singapore Med J, 2003 Apr;44(4):194-6.
    PMID: 12952031
    Four hundred and six AIDS patients were recruited in this retrospective study. The seroprevalence of toxoplasmosis among 406 AIDS patients was 208 (51.2%). Their age ranged from 17 to 74 years with a median of 35 years. The majority of patients were males 172 (82.6%), Malays 99 (47.5%), single 109 (52.4%), unemployed 99 (47.6%) and heterosexual with commercial sex workers (CSW) 97 (46.6%) as the risk marker to HIV infection. Thirty-one (14.9%) of 208 AIDS-related toxoplasmosis were diagnosed as active toxoplasmic encephalitis. The most common clinical manifestation was headache (67.7%). The CT scan findings showed most lesions to be multiple (87.5%), hypodense (66.7%), and in frontal region (41.7%). Twenty-two (71%) patients had chronic (latent) Toxoplasma infection as evidenced by seropositivity for anti-Toxoplasma (IgG) antibody. They were statistically significant in the association between CD4 count and toxoplasmic encephalitis (P = 0.019; OR = 2.6; 95% CI = 1.14-6.02). After the initial six weeks of anti-TE therapy, relapsing toxoplasmic encephalitis was detected in 9.7% in this study.
    MeSH terms: Adolescent; Adult; Aged; Encephalitis/epidemiology; Encephalitis/parasitology; Encephalitis/radiography; Female; Humans; Male; Middle Aged; Retrospective Studies; Singapore/epidemiology; Tomography, X-Ray Computed; Toxoplasmosis/epidemiology*; Toxoplasmosis/radiography; Seroepidemiologic Studies; Toxoplasmosis, Cerebral/epidemiology; Age Distribution
  12. Nawawi H, Osman NS, Yusoff K, Khalid BA
    Horm Metab Res, 2003 Aug;35(8):479-85.
    PMID: 12953165 DOI: 10.1055/s-2003-41805
    Hypercholesterolemia causes endothelial dysfunction, an early feature of atherosclerosis, leading to increased production of adhesion molecules and cytokines. The aim of this study was to investigate the effects of three months of treatment with low dose atorvastatin on serum levels of adhesion molecules, interleukin-6 (IL-6) and highly sensitive C-reactive protein (hs-CRP) in patients with non-familial hypercholesterolemia. Fifty-five patients with non-familial hypercholesterolemia were randomized to treatment with atorvastatin 10 mg/day or placebo for 3 months. Soluble intercellular adhesion molecules-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), E-selectin, IL-6 and hs-CRP levels were measured to assess the inflammatory activity of the endothelium. There was a significant reduction in ICAM-1 at 2 weeks (p<0.0001) with further reduction at 3 months (p<0.0001). At 3 months, there were significant reductions in VCAM-1 (p<0.02), IL-6 (p<0.0001) and hs-CRP (p<0.01), but an increase in E-selectin levels (p<0.002). Treatment with statin was an independent determinant of change in ICAM-1 (p<0.05) and IL-6 levels (p<0.05) after correcting for anthropometric indices, blood pressure and lipid profile. Low-dose atorvastatin treatment leads to reduction in proinflammatory markers of endothelial function, suggesting an attenuation of endothelial activation and improvement in endothelial function, independent of lipid lowering. This may lead to a reduction in the progression of atherosclerosis.
    MeSH terms: Atorvastatin Calcium; C-Reactive Protein/metabolism*; Dose-Response Relationship, Drug; Double-Blind Method; Drug Administration Schedule; Female; Heptanoic Acids/administration & dosage*; Humans; Hypercholesterolemia/blood*; Hypercholesterolemia/drug therapy*; Lipids/blood; Male; Middle Aged; Pyrroles/administration & dosage*; Interleukin-6/antagonists & inhibitors; Interleukin-6/blood*; Multivariate Analysis; Intercellular Adhesion Molecule-1/blood*; Vascular Cell Adhesion Molecule-1/blood*; E-Selectin/metabolism; Hydroxymethylglutaryl-CoA Reductase Inhibitors/administration & dosage*
  13. Arshad AR
    Head Neck, 2003 Sep;25(9):754-7.
    PMID: 12953311
    Kimura's disease is a chronic inflammatory disorder of unknown etiology commonly seen among orientals and characterized histologically by lymphatic follicles, vascular proliferation, and marked eosinophilic infiltration. It has a predilection for the head and neck region. The lesion is benign but can be mistaken to be a malignant lesion.
    MeSH terms: Adolescent; Adult; Angiolymphoid Hyperplasia with Eosinophilia/drug therapy*; Angiolymphoid Hyperplasia with Eosinophilia/pathology; Angiolymphoid Hyperplasia with Eosinophilia/surgery*; Diagnosis, Differential; Edema/drug therapy; Edema/surgery; Humans; Male; Middle Aged; Parotid Diseases/drug therapy*; Parotid Diseases/pathology; Parotid Diseases/surgery*; Steroids/therapeutic use; Treatment Outcome
  14. Prepageran N, Krishnan G
    Singapore Med J, 2003 Mar;44(3):123-5.
    PMID: 12953724
    To present our experience of endoscopic electrocoagulation of sphenopalatine artery for persistent posterior epistaxis despite conservative measures.
    MeSH terms: Adult; Aged; Epistaxis/therapy*; Female; Humans; Male; Nasal Cavity/blood supply*; Nasopharynx/blood supply*; Retrospective Studies
  15. Bahari MB, Nur NM, Rahman AF
    Singapore Med J, 2003 Mar;44(3):131-5.
    PMID: 12953726
    A survey was carried out among primary school teachers in the district of Kota Bharu, Malaysia to assess the level of knowledge on asthma and its management. Our findings revealed that primary school teachers were less informed about the management and treatment of asthma. They were relatively more knowledgeable about the causes and symptomatology of asthma. The majority of respondents had misunderstanding regarding the effect of rain, smoking and cold weather on asthma. It is important that teachers should be able to recognise symptoms of an asthmatic attack or take the necessary precautions to avoid such an attack. Many teachers agreed on the need to have an asthma education programme in their teaching curriculum.
    MeSH terms: Asthma*; Child; Cross-Sectional Studies; Health Education*; Humans; Health Knowledge, Attitudes, Practice*; Malaysia; Schools; Knowledge*; School Teachers*
  16. Saffor A, bin Ramli AR, Ng KH
    Australas Phys Eng Sci Med, 2003 Jun;26(2):39-44.
    PMID: 12956184
    Wavelet-based image coding algorithms (lossy and lossless) use a fixed perfect reconstruction filter-bank built into the algorithm for coding and decoding of images. However, no systematic study has been performed to evaluate the coding performance of wavelet filters on medical images. We evaluated the best types of filters suitable for medical images in providing low bit rate and low computational complexity. In this study a variety of wavelet filters are used to compress and decompress computed tomography (CT) brain and abdomen images. We applied two-dimensional wavelet decomposition, quantization and reconstruction using several families of filter banks to a set of CT images. Discreet Wavelet Transform (DWT), which provides efficient framework of multi-resolution frequency was used. Compression was accomplished by applying threshold values to the wavelet coefficients. The statistical indices such as mean square error (MSE), maximum absolute error (MAE) and peak signal-to-noise ratio (PSNR) were used to quantify the effect of wavelet compression of selected images. The code was written using the wavelet and image processing toolbox of the MATLAB (version 6.1). This results show that no specific wavelet filter performs uniformly better than others except for the case of Daubechies and bi-orthogonal filters which are the best among all. MAE values achieved by these filters were 5 x 10(-14) to 12 x 10(-14) for both CT brain and abdomen images at different decomposition levels. This indicated that using these filters a very small error (approximately 7 x 10(-14)) can be achieved between original and the filtered image. The PSNR values obtained were higher for the brain than the abdomen images. For both the lossy and lossless compression, the 'most appropriate' wavelet filter should be chosen adaptively depending on the statistical properties of the image being coded to achieve higher compression ratio.
    MeSH terms: Algorithms; Animals; Brain/radiography*; Fourier Analysis; Humans; Quality Control; Radiography, Abdominal/methods*; Signal Processing, Computer-Assisted*; Tomography, X-Ray Computed/methods*; Information Storage and Retrieval/methods*
  17. Suzina AH, Hamzah M, Samsudin AR
    J Laryngol Otol, 2003 Aug;117(8):605-8.
    PMID: 12956913
    The differences in facial anatomical structures of the major ethnic groups, may also be reflected in nasal resistance. Active anterior rhinomanometry (AAR) is the recommended technique for the objective assessment of nasal airway resistance (NAR). This study comprised of 85 adult Malay subjects. All the subjects had to undergo a primary assessment of relevant symptoms of nasal disease and nasal examination before undergoing AAR assessment. The mean value of total nasal airway resistance (NAR) was 0.19 Pa/cm(3)/s (ranged from 0.09 to 0.55 Pa/cm(3)/s) at 75 Pa pressure point and 0.24 Pa/cm(3)/s (ranged from 0.12 to 0.52 Pa/cm(3)/s) at 150 Pa pressure point. The mean unilateral NAR was 0.46 Pa/cm(3)/s at a reference pressure of 75 Pa and 0.51 Pa/cm(3)/s at a reference pressure of 150 Pa. In this study we presented normal values for NAR in healthy Malay adult subjects. AAR proves to be a valuable clinical method for recording and quantitating nasal resistance.
    MeSH terms: Adolescent; Adult; Aged; Airway Resistance/physiology*; Body Height/physiology; Female; Humans; Malaysia; Male; Middle Aged; Nose/physiology*; Pressure; Smoking/physiopathology; Rhinomanometry/methods*
  18. Suzina AH, Hamzah M, Samsudin AR
    J Laryngol Otol, 2003 Aug;117(8):609-13.
    PMID: 12956914
    Nasal obstruction is a subjective complaint in patients with nasal disease. The ability to quantitate the nasal ventilation dysfunction would be useful for making the appropriate choice of nasal disease management. This cross-sectional study comprised of 200 adult subjects. They underwent assessment of relevant symptoms, nasal examination and investigations before undergoing active anterior rhinomanometry (AAR) assessment. A group of 88 normal subjects and 112 patients with nasal disease were included. The mean total nasal air resistance (NAR) was significantly higher in patients with nasal disease (0.33 Pa/cm(3)/s) as compared to normal subjects (0.24 Pa/cm(3)/s). There was no significant difference in total NAR between patients with symptoms of nasal obstruction and those without the symptoms (p = 0.42). It is concluded that AAR is a sensitive but not a specific tool for the detection of abnormalities in NAR and it failed to relate to the symptom of nasal obstruction.
    MeSH terms: Adolescent; Adult; Airway Resistance/physiology*; Cross-Sectional Studies; Humans; Nasal Septum/physiopathology; Nose/physiopathology; Nose Diseases/diagnosis*; Nose Diseases/physiopathology; Pressure; Rhinitis, Allergic, Perennial/diagnosis; Rhinitis, Allergic, Perennial/physiopathology; Nasal Obstruction/diagnosis; Nasal Obstruction/physiopathology; Rhinomanometry/methods*
  19. Lim J, Lal S, Ng KC, Ng KS, Saha N, Heng CK
    Int J Cardiol, 2003 Aug;90(2-3):269-73.
    PMID: 12957761
    BACKGROUND: Polymorphisms of the glycoprotein IIIa receptor have been shown to be associated with differences in platelet aggregability. The PI(A2) variant of the polymorphism has been reported to be an inherited risk factor for acute coronary events. Although the allele frequency of this polymorphism is well documented in Caucasian populations, studies involving Asian Indians, Malays and Chinese are lacking. We studied 706 random male individuals to determine the genotypic distribution of this polymorphism in Singapore.

    METHODS: Male subjects included in this study were drawn from those undergoing routine annual medical examinations offered by their employers. Venous blood was obtained from these patients after an overnight fast and from which genomic DNA was extracted. Genotyping was carried out by polymerase chain reaction (PCR) followed by digestion with restriction enzyme NciI. Personal and family medical history of the subjects were also taken.

    RESULTS: The genotype distribution of the individuals studied was in accordance to a population at Hardy Weinberg equilibrium. The frequency of the PI(A2) allele was 0.1, 0.01 and 0.01 in the Indians, Malays and Chinese, respectively. The differences in frequencies of the PI(A2) variant are significant among different ethnic groups (P<0.001 for Indians vs. Chinese and Indians vs. Malays).

    CONCLUSIONS: We observed a significantly higher frequency of the PI(A2) allele among Indians relative to the Chinese and Malays in Singapore. The effect of this genotype may partially explain the higher rate of ischaemic heart disease seen among Indians compared to the Chinese and Malay ethnic groups.

    MeSH terms: Adult; China/ethnology; Coronary Disease/genetics; Gene Frequency*; Genetics, Population; Genotype; Humans; India/ethnology; Malaysia/ethnology; Male; Polymorphism, Genetic; Singapore; Chi-Square Distribution; Polymerase Chain Reaction; Integrin beta3/genetics*; Asian Continental Ancestry Group/genetics*
  20. Liu Y, Lee MA, Ooi EE, Mavis Y, Tan AL, Quek HH
    J Clin Microbiol, 2003 Sep;41(9):4388-94.
    PMID: 12958274
    A multiplex PCR method incorporating primers flanking three variable-number tandem repeat (VNTR) loci (arbitrarily labeled TR1, TR2, and TR3) in the CT18 strain of Salmonella enterica serovar Typhi has been developed for molecular typing of S. enterica serovar Typhi clinical isolates from several Asian countries, including Singapore, Indonesia, India, Bangladesh, Malaysia, and Nepal. We have demonstrated that the multiplex PCR could be performed on crude cell lysates and that the VNTR banding profiles produced could be easily analyzed by visual inspection after conventional agarose gel electrophoresis. The assay was highly discriminative in identifying 49 distinct VNTR profiles among 59 individual isolates. A high level of VNTR profile heterogeneity was observed in isolates from within the same country and among countries. These VNTR profiles remained stable after the strains were passaged extensively under routine laboratory culture conditions. In contrast to the S. enterica serovar Typhi isolates, an absence of TR3 amplicons and a lack of length polymorphisms in TR1 and TR2 amplicons were observed for other S. enterica serovars, such as Salmonella enterica serovar Typhimurium, Salmonella enterica serovar Enteritidis, and Salmonella enterica serovar Paratyphi A, B, and C. DNA sequencing of the amplified VNTR regions substantiated these results, suggesting the high stability of the multiplex PCR assay. The multiplex-PCR-based VNTR profiling developed in this study provides a simple, rapid, reproducible, and high-resolution molecular tool for the epidemiological analysis of S. enterica serovar Typhi strains.
    MeSH terms: Alleles; Asia; Humans; Salmonella typhi/classification*; Salmonella typhi/genetics; Sensitivity and Specificity; Genetic Variation; Bacterial Typing Techniques/methods*; Minisatellite Repeats*
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