Browse publications by year: 2004

  1. Ito F, Hashim R, Huei YS, Kaufmann E, Akino T, Billen J
    Naturwissenschaften, 2004 Oct;91(10):481-4.
    PMID: 15729761
    The mechanism by which palatable species take advantage of their similarity in appearance to those that are unpalatable, in order to avoid predation, is called Batesian mimicry. Several arthropods are thought to be Batesian mimics of social insects; however, social insects that are Batesian mimics among themselves are rare. In Malaysia we found a possible Batesian mimic in an arboreal ant species, Camponotus sp., which was exclusively observed on foraging trails of the myrmicine ant Crematogaster inflata. The bright yellow and black colouring pattern, as well as the walking behaviour, were very similar in both species. We observed general interactions between the two species, and tested their palatability and the significance of the remarkably similar visual colour patterns for predator avoidance. Prey offered to C. inflata was also eaten by Camponotus workers in spite of their being attacked by C. inflata, indicating that Camponotus sp. is a commensal of C. inflata. An experiment with chicks as potential predators suggests that Camponotus sp. is palatable whereas C. inflata is unpalatable. After tasting C. inflata, the chicks no longer attacked Camponotus sp., indicating that Camponotus sp. is a Batesian mimic of Crematogaster inflata.
    MeSH terms: Adaptation, Biological; Adaptation, Physiological; Aggression; Animals; Ants/physiology*; Feeding Behavior*; Predatory Behavior
  2. Chisholm LA, Whittington ID
    Folia Parasitol., 2004 Dec;51(4):304-10.
    PMID: 15729942
    Myliocotyle borneoensis sp. n. and M. multicrista sp. n. (Monocotylidae: Heterocotylinae) are described from the gills of the mottled eagle ray, Aetomylaeus maculatus (Gray), and the banded eagle ray A. nichofii (Bloch et Schneider) (Myliobatidae), respectively, collected from the northern coast of Malaysian Borneo. These are the first monogeneans to be described on elasmobranchs from Borneo. The formerly monotypic Myliocotyle (for M. pteromylaei) was distinguished from other monocotylids by the distribution and morphology of the eight sclerotised dorsal haptoral accessory structures and the morphology of the male copulatory organ. However, we have determined that M. pteromylaei has ten structures on the dorsal surface of the haptor. Myliocotyle borneoensis is distinguished from M. pteromylaei by the morphology of the male copulatory organ and its accessory piece. Myliocotyle multicrista has 12 sclerotised dorsal haptoral accessory structures and a male copulatory organ with two accessory pieces. Additional sclerotised ridges across the ventral surfaces of each loculus (except the posterior-most pair) are also present in M. multicrista. The diagnosis for Myliocotyle is revised given our discovery of additional dorsal haptoral accessory structures in the type species and to accommodate other new characters of the two new species. Anterior secretions of Myliocotyle are discussed.
    MeSH terms: Animals; Borneo; Female; Gills/parasitology; Male; Microscopy, Phase-Contrast; Skates (Fish)/parasitology*; Trematoda/anatomy & histology*
  3. Cherenet T, Sani RA, Panandam JM, Nadzr S, Speybroeck N, van den Bossche P
    Onderstepoort J Vet Res, 2004 Dec;71(4):307-12.
    PMID: 15732457
    During a period of four consecutive years, trypanosomosis surveys were conducted in a tsetse-infested and tsetse-free area of the Amhara Region of north-west Ethiopia. In each study area randomly selected communal cattle were sampled and their blood was investigated using parasitological diagnostic methods. At the same time the population of biting flies was sampled. The monthly average prevalence of trypanosome infections in cattle did not differ significantly between study areas. In both study areas, the prevalence of trypanosome infections was highest during the long rainy season. Trypanosome infections were mainly due to Trypanosoma vivax and they significantly reduced the average packed cell volume and the body condition of the animals. The monthly prevalence of infection was correlated with the density of biting flies, such as Tabanidae and Stomoxys spp., in the preceding month suggesting an important role of mechanical transmission in the epidemiology of trypanosomosis in both areas.
    MeSH terms: Animals; Cattle; Ethiopia/epidemiology; Female; Male; Rain; Seasons; Trypanosoma brucei brucei/isolation & purification; Trypanosoma congolense/isolation & purification; Trypanosomiasis, African/epidemiology; Trypanosomiasis, African/transmission; Trypanosomiasis, African/veterinary; Trypanosomiasis, Bovine/epidemiology*; Trypanosomiasis, Bovine/transmission; Tsetse Flies/parasitology*; Prevalence; Trypanosoma vivax/isolation & purification
  4. Wong ML, Che Fatimah Awang, Ng LK, Norlian D, Rashidah Dato Burhanudin, Gere MJ
    Singapore Dent J, 2004 Dec;26(1):10-4.
    PMID: 15736836
    Early orthodontic interventions are often initiated in the developing dentition to promote favourable developmental changes and remove or suppress those that are unfavourable. Early interceptive orthodontics can eliminate or reduce the severity of a developing malocclusion, the complexity of orthodontic treatment, overall treatment time and cost. It also improves self-esteem in the subjects and parental satisfaction. Early detection and appropriate referral of cases requiring interceptive orthodontics are important. However, lack of awareness among school children, parents and primary-care personnel (dental nurses and dental officers) may result in patients not being referred for timely interceptive intervention. This article presents a general view of the scope of interceptive orthodontics that can be carried out in early mixed dentition, i.e. when the permanent incisors and molars are erupting into the oral cavity.
    MeSH terms: Age Factors; Child; Dentition, Mixed; Diastema/prevention & control; Habits; Humans; Malocclusion/prevention & control; Orthodontics, Interceptive*; Space Maintenance, Orthodontic; Tooth Eruption, Ectopic/prevention & control
  5. Nik-Hussein NN, Abdul Muttalib K, Junid NZ, Wan MN, Abang A
    Singapore Dent J, 2004 Dec;26(1):30-8.
    PMID: 15736839
    The aim of the present study was to determine the oral health status of 16-year-old Malaysian school children. The prevalence of caries was 75.5%. More than 60% of subjects had caries experience by teeth (DMFT) scores of 0-3. The largest component of the DMFT and caries experience by surface index was the filled component. Females had higher caries prevalence and caries scores than males. The mouth and tooth prevalences of enamel defects were 56% and 21.8%, respectively. The most common type of enamel defects observed were diffuse opacities, affecting 95.5% of affected subjects and 92.1% of affected teeth. One-third of subjects had healthy gingival conditions, 8.6% had bleeding gingivae, 55.1% had calculus and 3% had pockets. Less than 1% of subjects wore or required dentures. Cleft lip and/or palate was uncommon. This study shows that the prevalence of caries and DMFT scores have declined over the last 30 years.
    MeSH terms: Adolescent; Dental Caries/epidemiology*; Dental Enamel Hypoplasia/epidemiology; DMF Index; Female; Humans; Incisor/injuries; Malaysia/epidemiology; Male; Oral Health; Periodontal Diseases/epidemiology*; Periodontal Index; Tooth Fractures/epidemiology; Prevalence
  6. Jantan I, Pisar M, Sirat HM, Basar N, Jamil S, Ali RM, et al.
    Phytother Res, 2004 Dec;18(12):1005-7.
    PMID: 15742349
    Ten compounds isolated from Alpinia mutica Roxb., Curcuma xanthorrhiza Roxb. and Kaempferia rotunda Linn. (Family: Zingiberaceae) were investigated for their platelet-activating factor (PAF) antagonistic activities on rabbit platelets using 3H-PAF as a ligand. Among them, four compounds showed significant inhibitory effects. Alpinetin and 5,6-dehydrokawain isolated from A. mutica exhibited IC50 values of 41.6 and 59.3 microM, respectively. The IC50 values of 3-deacetylcrotepoxide and 2-hydroxy-4,4',6'-trimethoxychalcone from K. rotunda were 45.6 and 57.4 microM, respectively. 1-Methoxy-2-methyl-5-(1',5'-dimethylhex-4'-enyl)-benzene, synthesized by methylation of xanthorrhizol which was obtained from C. xanthorrhiza, showed an IC50 value of 40.9 microM. The results indicated that these compounds were relatively strong PAF receptor binding inhibitors.
    MeSH terms: Animals; Blood Platelets/drug effects*; Blood Platelets/physiology; Fruit; Phytotherapy*; Plant Extracts/administration & dosage; Plant Extracts/pharmacology*; Plant Extracts/therapeutic use; Platelet Activating Factor/antagonists & inhibitors*; Platelet Aggregation Inhibitors/administration & dosage; Platelet Aggregation Inhibitors/pharmacology*; Platelet Aggregation Inhibitors/therapeutic use; Rabbits; Inhibitory Concentration 50; Rhizome; Curcuma; Zingiberaceae*; Alpinia
  7. Edwards JR
    Dev Biol (Basel), 2004;119:423-31.
    PMID: 15742655
    The OIE Southeast Asia Foot-and-Mouth Disease Campaign (SEAFMD) involves the coordinated control of foot-and-mouth disease by eight of the ASEAN countries. A long term vision for SEAFMD has been developed and the core element is a progressive zoning approach to the control and eradication of FMD in the region. This paper describes the current status of FMD in Southeast Asia and progress towards achievement of OIE free zone status for FMD in parts of the Philippines and Malaysia and the initiation of the Malaysia-Thailand-Myanmar (MTM) Peninsular Campaign for FMD Freedom. In mainland Southeast Asia, the progressive zoning approach involves several sub-regional groups working in parallel to oversee the epidemiological and economic studies required to determine the feasibility of the approach. Areas involved include the Lower Mekong Basin, Upper Mekong Basin, parts of Myanmar and the Red River Delta of Vietnam. The paper describes the current usage of vaccines for FMD in Southeast Asia and provides recommendations for their supply and use in the new regional initiatives.
    MeSH terms: Animals; Asia, Southeastern/epidemiology; Cost-Benefit Analysis; Disease Outbreaks/prevention & control; Disease Outbreaks/veterinary*; Foot-and-Mouth Disease/economics; Foot-and-Mouth Disease/epidemiology; Foot-and-Mouth Disease/prevention & control*; Vaccination/veterinary*; Epidemiologic Studies
  8. Kaku Y
    Uirusu, 2004 Dec;54(2):237-42.
    PMID: 15745162
    Nipah virus (NiV), emerged in Peninsular Malaysia, caused an outbreak of severe febrile encephalitis in humans and respiratory diseases in pigs between 1998 and 1999. By May of 1999, the death of 105 humans and the culling of about 1.1 million pigs were reported. Fruitbats of Pteropid species were identified as the natural reservoir hosts. The epidemiological studies suggested that NiV was introduced into pig farms by fruitbats, and was than transmitted to humans (mainly pig farmers) and other animals such as dogs, cats and horses. In 2004, NiV reappeared in Bangladesh with greater lethality. In contrast to the Malaysia case, epidemiologic characteristics of this outbreak suggested the possibility of fruitbats-to-person, or person-to-person transmission. In this article, the epidemiological comparison between two outbreaks in Malaysia and Bangladesh, and the new-trends of virological studies of NiV will be discussed.
    MeSH terms: Animals; Bangladesh/epidemiology; Cats; Chiroptera/virology; Disease Outbreaks; Disease Vectors; Dogs; Humans; Malaysia/epidemiology; Seasons; Swine; Zoonoses/epidemiology*; Zoonoses/transmission*; Zoonoses/virology; Nipah Virus*; Henipavirus Infections/epidemiology*; Henipavirus Infections/prevention & control; Henipavirus Infections/transmission; Henipavirus Infections/virology
  9. Harum H
    PMID: 15747961
    Malaysia's experience in implementing the Integrated Telehealth Project has placed her way ahead in the arena of world Telehealth. Thus, she has become the focus point, reference point and benchmark for similar endeavors around the world. In fact, it would not be presumptuous to state that the Integrated Telehealth project is a trail-blazing pioneer with e-leadership experience and skills developed over the last few years. It is hoped that the Integrated Telehealth concept will find acceptance and credence globally.
    MeSH terms: Developing Countries; Humans; Information Systems/trends*; Leadership*; Malaysia/epidemiology; National Health Programs/organization & administration*; Telecommunications/trends; Program Development*; Telemedicine/organization & administration*; Systems Integration; Health Transition; Internet*
  10. Harum H
    PMID: 15747966
    The Integrated Telehealth Project of Malaysia is considered a principal enabler for the nation's Vision 2020 as well as the National Health Vision. Being in such an unenviable position, of being not only the pioneer for such an integrated project, but also with no benchmark to compare with, the project implementers have faced manifold challenges along the way. This chapter deals with some of the challenges and lessons learnt that have accumulated as the project progressed.
    MeSH terms: Computer Communication Networks; Humans; Malaysia; National Health Programs*; Software; Program Development*; Telemedicine/organization & administration*; Systems Integration; Delivery of Health Care, Integrated*
  11. Suleiman AB
    Stud Health Technol Inform, 2004;104:182-90.
    PMID: 15747978 DOI: 10.3233/978-1-60750-947-9-182
    Malaysia, like many other countries faces major challenges in meeting increasing demands with limited resources. Changes in demography, life-style diseases, increasing consumer expectations, new medical technologies and rapid economic growth all fuel demand for more healthcare services. There are problems related to the distribution and delivery of healthcare services, and there is inadequate integration of healthcare delivery and continuity of care is a major concern. Resources tend to be concentrated in the very expensive hospital sector, although services would be cost-effectively and conveniently delivered at primary care level. There is no ideal healthcare system, and how healthcare is supported and organized for service delivery influences the country's social, economic and political well-being. Like many other countries, Malaysia is undergoing health reform in meeting these challenges, and is becoming more reliant on telemedicine and telehealth.
    MeSH terms: Continuity of Patient Care; Health Resources/economics; Health Resources/supply & distribution; Humans; Malaysia; National Health Programs*; Program Development*; Health Care Costs; Models, Organizational; Private Sector; Public Sector; Delivery of Health Care, Integrated/organization & administration*; Remote Consultation/economics; Remote Consultation/organization & administration*; Universal Coverage
  12. Nesaretnam K, Ambra R, Selvaduray KR, Radhakrishnan A, Canali R, Virgili F
    Ann N Y Acad Sci, 2004 Dec;1031:143-57.
    PMID: 15753141
    Vitamin E is important not only for its cellular antioxidant and lipid-lowering properties, but also as an antiproliferating agent. It has also been shown to contribute to immunoregulation, antibody production, and resistance to implanted tumors. It has recently been shown that tocotrienols are the components of vitamin E responsible for growth inhibition in human breast cancer cells in vitro as well as in vivo through estrogen-independent mechanisms. Although tocotrienols act on cell proliferation in a dose-dependent manner and can induce programmed cell death, no specific gene regulation has yet been identified. In order to investigate the molecular basis of the effect of a tocotrienol-rich fraction (TRF) from palm oil, we performed a cDNA array analysis of cancer-related gene expression in estrogen-dependent (MCF-7) and estrogen-independent (MDA-MB-231) human breast cancer cells. The human breast cancer cells were incubated with or without 8 mug/mL of tocotrienols for 72 h. RNA was subsequently extracted and subjected to reverse transcription before being hybridized onto cancer arrays. Tocotrienol supplementation modulated significantly 46 out of 1200 genes in MDA-MB-231 cells. In MCF-7 cells, tocotrienol administration was associated with a lower number of affected genes. Interestingly, only three were affected in a similar fashion in both cell lines: c-myc binding protein MM-1, 23-kDa highly basic protein, and interferon-inducible protein 9-27 (IFITM-1). These proteins are most likely involved in the cell cycle and can exert inhibitory effects on cell growth and differentiation of the tumor cell lines. These data suggest that tocotrienols are able to affect cell homeostasis, possibly independent of their antioxidant activity.
    MeSH terms: Antioxidants; Breast Neoplasms; Cell Cycle/drug effects; Cell Cycle/genetics; Estrogens/pharmacology; Humans; Nucleic Acid Hybridization; Plant Oils/chemistry*; RNA/analysis; RNA/isolation & purification; Tumor Cells, Cultured; Gene Expression Regulation, Neoplastic/drug effects*; DNA, Complementary/analysis; Tocotrienols/analysis; Tocotrienols/pharmacology*
  13. Foo LH, Khor GL, Tee ES, Dhanaraj P
    Int J Food Sci Nutr, 2004 Sep;55(6):517-25.
    PMID: 15762316
    Iron deficiency anaemia is the most common micronutrient deficiency worldwide. The prevalence of anaemia in the developing countries is three to four times higher than that in the developed countries. The iron status was assessed in 199 apparently healthy male and female adolescents aged 12-19 years living in a fishing community in Sabah, Malaysia. Data on socio-economic characteristics, lifestyles, anthropometry measurements, iron status, and dietary intake were gathered. Dietary intake of energy, iron, and most nutrients (with the exception of protein and vitamin C) were below the recommended levels for Malaysian adolescents. Three-quarters of the iron was derived from plant foods. The mean haemoglobin value for the male was 13.9 +/- 1.3 g/dl with 9.5% having less than 12 g/dl, while the respective figures for the female were 12.4 +/- 1.6 g/dl and 28.6%. The mean serum ferritin concentrations for male and female adolescents were 21.5 and 15.4 microg/l, respectively; with 25.7% of the males and 49.5% of the females having deficient levels of ferritin. Dietary intake of total energy and iron, and gender were found to be independent determinants of serum ferritin and haemoglobin levels, accounting for over 40% of the variations for each of these iron indicators. In males, but not in females, the intake of dietary protein and iron, and physical activity were also found to be significant determinants of serum ferritin. The age of subjects and household size were significant determinants of haemoglobin levels for male subjects, but not for female subjects. The findings indicate the importance of adequate intake of energy and dietary iron for improving the iron status of adolescents.
    MeSH terms: Adolescent; Adult; Anthropometry; Energy Intake/physiology; Child; Developing Countries*; Diet/statistics & numerical data; Female; Ferritins/blood; Hemoglobins/analysis; Humans; Iron/blood; Life Style; Malaysia/epidemiology; Male; Motor Activity/physiology; Rural Health/statistics & numerical data*; Sex Factors; Socioeconomic Factors; Transferrin/analysis; Anemia, Iron-Deficiency/etiology; Anemia, Iron-Deficiency/epidemiology*; Anemia, Iron-Deficiency/prevention & control; Iron, Dietary/administration & dosage*
  14. Rahman AJA
    Med J Malaysia, 2004 Oct;59(4):441-2.
    PMID: 15779574
    MeSH terms: Molecular Biology/trends*
  15. Low WY
    Med J Malaysia, 2004 Oct;59(4):443-9.
    PMID: 15779575 MyJurnal
    A sexual health course was offered and taught by academic staff from the Faculty of Medicine, University of Malaya during semester II of every year as a university elective course to other university students apart from medical students. The course covered a wide range of topics: adolescent sexuality, family planning and pregnancy, violence against women, alternative sexual behavior, physiology of sex, sex and the disabled, gender bias in sexuality, relationship and marriage, sexual dysfunctions, clarification of sexual attitudes and STDs and AIDS. The Sexual Knowledge and Attitude Test (SKAT-II) was used to measure students' pre- and post-course scores on sexual knowledge and attitudes. Fifty-four students who completed both the pre- and post-course tests showed a significant change in sexual knowledge and their attitudes towards sexual myths and autoeroticism. Sexual knowledge was also positively correlated with age, heterosexual relations, autoeroticism and sexual myths scores. However, sexual knowledge is negatively related to religiosity and the influence of religious beliefs on one's attitudes towards sexual matters. This study showed that the sexual health course offered does have a positive impact in increasing one's knowledge and changing one's attitudes towards sexual issues.
    MeSH terms: Female; Folklore; Humans; Health Knowledge, Attitudes, Practice; Malaysia; Male; Sex Education*; Students/psychology; Universities
  16. Yeap JS, Lee DJK, Fazir M, Kareem BA, Yeap JK
    Med J Malaysia, 2004 Oct;59(4):450-4.
    PMID: 15779576
    A retrospective study was conducted to determine the incidence of nerve injuries in anterior dislocations of the shoulder and to determine if a neurological examination is routinely performed in such cases. One hundred and fifteen shoulder dislocations in 100 patients (74 males, 26 females; mean age 35+/-18 years) were reviewed. Eighty-seven patients were examined for neurological injuries prior to reduction and 8 of these patients (9.2%) were found to have sustained neurological injuries. Following reduction, neurological examination was performed in 85 patients. Three patients who were not examined initially prior to reduction were subsequently found to have nerve injuries after reduction. Seven patients (7%) were not examined for neurological injury both before and after reduction of the dislocation. Eleven patients were found to have sustained nerve injuries in the final analysis. Axillary nerve injuries were the most common, occurring in 8 patients. In conclusion, nerve injuries are common in shoulder dislocations. The importance of performing and documenting the findings of neurological examination anterior shoulder dislocations needs to be reiterated.
    MeSH terms: Adolescent; Adult; Aged; Aged, 80 and over; Female; Humans; Male; Middle Aged; Retrospective Studies; Shoulder Dislocation/complications*; Peripheral Nerve Injuries*
  17. Koay TK, Nirmal S, Noitie L, Tan E
    Med J Malaysia, 2004 Oct;59(4):455-9.
    PMID: 15779577
    From October 13 to October 21, 1999, a total of 46 males, aged 8 to 19 years, were admitted to Beaufort Hospital after swimming in the creek near an oil palm plantation in Kampung (Kg) Kebatu, Beaufort. Thirty (30) presented with symptoms including fever, vomiting, bodyache, giddiness, headache, chest pain and cough, while 16 others, were asymptomatic. One, a 15 year old boy, died from haemorrhagic shock secondary to pulmonary haemorrhage. The onset of the illness was from 11 October to 19 October 1999. A case-control study found that the outbreak was associated with swimming in the creek (p<0.0001). A total of 44 paired sera samples were sent for microscopic agglutination test (MAT), 5 pairs showed sero-conversion, 3 pairs had 4 fold rise in titre and 18 pairs were positive at >320. The findings indicated that Leptospirosis was the cause of the outbreak of this illness and the contaminated creek water was the source of the infection. The occurrence of flooding and stagnation in the creek following the heavy rainfall during the first week of October 1999 could have contributed to the timing of the outbreak.
    MeSH terms: Adolescent; Adult; Child; Disease Outbreaks*; Humans; Leptospirosis/transmission*; Malaysia; Swimming*; Water Pollution; Case-Control Studies
  18. Sadeghi M, Roohafza HR, Kelishadi R
    Med J Malaysia, 2004 Oct;59(4):460-7.
    PMID: 15779578
    This study was done to estimate the prevalence of high blood pressure (BP) in treated and non-treated subjects with respect to age and gender and its association with other cardiovascular risk factors in Iran. This cross sectional study was performed in three cities of Iran on participants over 19 years at 2002. First a questionnaire consisting of demographic details, drug intake and smoking status was filled. Then physical examination including systolic and diastolic blood pressure (SBP, DBP), body mass index (BMI) and waist to hip circumference (WHC) was performed. Fasting blood sample was drawn for sugar (FBS), total cholesterol (TC) and triglyceride (TG) and a 2-hour postprandial glucose was also measured. In this study performed on 12494 subjects, 48% were males and 52% females. The mean age of men and women was 38.99+/-15.30 and 38.80+/-14.54 years respectively. The prevalence of high BP in men and women was 15.6% and 18.8% respectively. The prevalence of high BP was higher in women than in men, except in the younger age classes. Overall 26.7% of hypertensive men and 47.7% of hypertensive women were on anti-hypertensive pharmacological treatment. Among the treated patients, BP was under control in 6.4% of the men and 13.8% of the women. In 86.5% of men with high BP and 89.3% of women with high BP, at least one other cardiovascular risk factor was present and its prevalence increased with age in both genders. BMI >25 (especially abdominal obesity) was the most frequent associated risk factor (41.9% in male, 59% in female). Except for smoking, the prevalence of each cardiovascular risk factor increased with the severity of hypertension, except in young women. The prevalence of high BP- even in treated subjects- is high in Iran. Many subjects with high BP have at least one other associated cardiovascular risk factor. These data emphasize the necessity of implementing community-based interventions.
    MeSH terms: Blood Pressure*; Cardiovascular Diseases/etiology*; Cross-Sectional Studies; Female; Humans; Hypertension/epidemiology; Iran/epidemiology; Male; Middle Aged; Risk Factors; Prevalence
  19. Zainuddin N, Jaafar H, Isa MN, Abdullah JM
    Med J Malaysia, 2004 Oct;59(4):468-79.
    PMID: 15779579
    Loss of heterozygosity (LOH) on several loci and mutations on PTEN tumor suppressor gene (10q23.3) occur frequently in sporadic gliomas. We have performed polymerase chain reaction (PCR)-LOH analysis using microsatellite markers and single-stranded conformational polymorphism (SSCP) analysis to determine the incidence of allelic losses on chromosome 10q, 9p, 17p and 13q and mutations of exons 5, 6 and 8 of the PTEN gene in malignant gliomas. Twelve of 23 (52.2%) malignant glioma cases showed allelic losses whereas 7 of 23, (30.4%) samples showed aberrant band patterns and mutations of the PTEN gene. Four of these cases showed LOH on 10q23 and mutations of the PTEN gene. The data on LOH indicated the involvement of different genes in gliomagenesis whereas mutations of the PTEN gene indicated the role of PTEN tumor suppressor gene in the progression of glioma in Malay population.
    MeSH terms: Brain Neoplasms/genetics*; Glioma/genetics*; Humans; Malaysia; Mutation*; Phosphoric Monoester Hydrolases/genetics*; Polymorphism, Single-Stranded Conformational; Microsatellite Repeats; Loss of Heterozygosity*; Tumor Suppressor Proteins/genetics*; PTEN Phosphohydrolase
  20. Jafri AM, Sarina S, George PJ, Nizam IM
    Med J Malaysia, 2004 Oct;59(4):480-5.
    PMID: 15779580 MyJurnal
    Recent study has shown that activation of the telomerase and p16 gene mutation are both necessary for tumorigenesis. Our objectives were to detect telomerase activity and investigate the possibility of p16 gene mutations in various types of brain tumor. We analyzed 23 tumor tissues collected in 2000 to 2002. Telomerase activity was detected by a TRAP assay using a TRAPEZE Telomerase Detection Kit (Intergen, Co). PCR-SSCP (Single Strand Conformation Polymorphism) analysis was performed to screen for p16 gene mutation at exon 1 and 2. The activity was detected in 26.1% of the brain tumor samples and mostly present in high-grade tumors. There was a significant association between telomerase activity status and tumor grade but not with patient criteria. Telomerase activity was detected in the analyzed tumors, supporting the fact that activation of telomerase is an important feature for tumorigenesis. There was no mobility shift of p16 gene using SSCP and suggested no mutation at exon 1 and 2 occurred in all samples. These results suggest that another mechanism of p16 gene alterations could be involved and associated with detectable telomerase activity in the progression of tumors.
    MeSH terms: Brain Neoplasms/genetics*; Enzyme Activation; Humans; Malaysia; Mutation*; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational; Telomerase/metabolism*; Genes, p16/physiology*
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