Browse publications by year: 2008

  1. Maruyama S, Minaguchi K, Takezaki N, Nambiar P
    Leg Med (Tokyo), 2008 May;10(3):160-2.
    PMID: 18180191 DOI: 10.1016/j.legalmed.2007.11.002
    Allele frequencies for 15 short tandem repeat (STR) loci D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA (AmpF/STR Identifiler PCR Amplification kit, PE Applied Biosystems) were obtained from a sample of 110 unrelated individuals from the Malay population living in and around Kuala Lumpur, Malaysia, and the characteristics of the population was compared with other East Asian populations.
    MeSH terms: Gene Frequency*; Genetics, Population*; Humans; Malaysia; Polymerase Chain Reaction; DNA Fingerprinting; Tandem Repeat Sequences*
  2. Shashinder S, Choo PK, Gopala KG
    Eur J Cancer Care (Engl), 2008 Jan;17(1):93-7.
    PMID: 18181897 DOI: 10.1111/j.1365-2354.2007.00814.x
    Being a rapidly developing country, a study was needed to see how we faired in treating head and neck cancer patients compared with international standards. Although being a retrospective study, this research shows that there is still a lot to be done in our developing nation in educating the general public about head and neck cancers as most of them presented in the later stages to us. There also needs to be a proper review about the treatment modality offered to patient as our survival results are far behind in certain categories of cancers compared with the developed nations.
    MeSH terms: Adolescent; Adult; Aged; Aged, 80 and over; Child; Child, Preschool; Developing Countries*; Epidemiologic Methods; Female; Head and Neck Neoplasms/mortality*; Head and Neck Neoplasms/therapy; Humans; Malaysia/ethnology; Malaysia/epidemiology; Male; Middle Aged
  3. Clements R, Liew TS, Vermeulen JJ, Schilthuizen M
    Biol Lett, 2008 Apr 23;4(2):179-82.
    PMID: 18182365 DOI: 10.1098/rsbl.2007.0602
    The manner in which a gastropod shell coils has long intrigued laypersons and scientists alike. In evolutionary biology, gastropod shells are among the best-studied palaeontological and neontological objects. A gastropod shell generally exhibits logarithmic spiral growth, right-handedness and coils tightly around a single axis. Atypical shell-coiling patterns (e.g. sinistroid growth, uncoiled whorls and multiple coiling axes), however, continue to be uncovered in nature. Here, we report another coiling strategy that is not only puzzling from an evolutionary perspective, but also hitherto unknown among shelled gastropods. The terrestrial gastropod Opisthostoma vermiculum sp. nov. generates a shell with: (i) four discernable coiling axes, (ii) body whorls that thrice detach and twice reattach to preceding whorls without any reference support, and (iii) detached whorls that coil around three secondary axes in addition to their primary teleoconch axis. As the coiling strategies of individuals were found to be generally consistent throughout, this species appears to possess an unorthodox but rigorously defined set of developmental instructions. Although the evolutionary origins of O. vermiculum and its shell's functional significance can be elucidated only once fossil intermediates and live individuals are found, its bewildering morphology suggests that we still lack an understanding of relationships between form and function in certain taxonomic groups.
    MeSH terms: Animals; Animal Structures/anatomy & histology*; Biological Evolution*; Malaysia; Species Specificity; Gastropoda/anatomy & histology*; Gastropoda/classification*
  4. Teoh CY, Lim FS
    Paediatr Anaesth, 2008 Feb;18(2):119-24.
    PMID: 18184242 DOI: 10.1111/j.1460-9592.2007.02385.x
    The Proseal laryngeal mask airway (PLMA) is a relatively new supraglottic airway device with a drain tube to minimize the risk of gastric insufflation and aspiration. We compared introducer tool (IT) and gum elastic bougie (GEB)-guided techniques for insertion of the Proseal LMA in children.
    MeSH terms: Anesthesia, Inhalation/instrumentation*; Anesthesia, Inhalation/methods; Child; Child, Preschool; Equipment Design; Female; Humans; Infant; Male; Pediatrics; Laryngeal Masks/adverse effects*
  5. Mohamed M, Diabcare-Asia 2003 Study Group
    Curr Med Res Opin, 2008 Feb;24(2):507-14.
    PMID: 18184454 DOI: 10.1185/030079908X261131
    OBJECTIVE: To collect information on diabetes management, including psychosocial aspects, in patients managed by specialists 5 years after they were first surveyed in 1998.

    METHODS: Data on demography, diabetes status, management and complications were collected via medical records, interview and laboratory assessments. HbA(1c) was analysed by a central laboratory prospectively.

    RESULTS: Patient profile was similar in the 1998 (N = 21,838) and 2003 cohorts (N = 15,549): 95% were diagnosed as type 2 diabetes mellitus and were obese (BMI approximately 25 kg/m(2)). Glycaemic control was unsatisfactory in many patients (mean HbA(1c) approximately 8%; fasting glucose approximately 9 mmol/L). Lipids were well-controlled but hypertension was not. The incidence of neuropathy ( approximately 33%) and cataract ( approximately 27%) were high. The majority ( approximately 71%) of patients in both cohorts were treated with oral antidiabetic drug (OAD) monotherapy; approximately 24% were on insulin therapy. Approximately half of the 2003 cohort reported a healthy state of well-being. Quality of life did not appear to have suffered as a result of having diabetes. However, many patients were worried about hypoglycaemic risk (53.9%) or worsening of diabetes (45.8%) and insulin initiation (64.5%).

    CONCLUSIONS: Although both cohorts were separate cross-sectional studies of diabetes management status in Asia, the results showed that the demography profile, glycaemic control and cardiovascular risk factors were remarkably similar in both cohorts 5 years after the first survey. More concerted efforts are needed to increase diabetes awareness and education.

    MeSH terms: Asia; Cross-Sectional Studies; Diabetes Mellitus, Type 2/drug therapy*; Diabetes Mellitus, Type 2/physiopathology; Diabetes Mellitus, Type 2/psychology; Female; Hemoglobin A, Glycosylated/drug effects; Humans; Hypertension/drug therapy; Hypertension/epidemiology; Hypoglycemic Agents/therapeutic use; Male; Medical Audit; Middle Aged; Obesity/epidemiology; Obesity/physiopathology*; Quality of Life; Risk Factors; Self Care; Incidence; Treatment Outcome; Disease Management
  6. Ng CH, Kong KC, Von ST, Balraj P, Jensen P, Thirthagiri E, et al.
    Dalton Trans, 2008 Jan 28.
    PMID: 18185860 DOI: 10.1039/b709269e
    A series of ternary metal(ii) complexes {M(phen)(edda); 1a (Cu), 1b (Co), 1c (Zn), 1d (Ni); H(2)edda = N,N(')-ethylenediaminediacetic acid} of N,N'-ethylene-bridged diglycine and 1,10-phenanthroline were synthesized and characterized by elemental analysis, FTIR, UV-visible spectroscopy and magnetic susceptibility measurement. The interaction of these complexes with DNA was investigated using CD and EPR spectroscopy. MTT assay results of 1a-1c , screened on MCF-7 cancer cell lines, show that synergy between the metal and ligands results in significant enhancement of their antiproliferative properties. Preliminary results from apoptosis and cell cycle analyses with flow cytometry are reported. seems to be able to induce cell cycle arrest at G(0)/G(1). The crystal structure of 1a is also included.
    MeSH terms: Animals; Antineoplastic Agents/chemical synthesis*; Antineoplastic Agents/pharmacology*; Antineoplastic Agents/chemistry; Cell Cycle/drug effects; Circular Dichroism; DNA/chemistry*; Electron Spin Resonance Spectroscopy; Humans; Intercalating Agents/chemical synthesis*; Intercalating Agents/pharmacology*; Intercalating Agents/chemistry; Ligands; Male; Metals/chemistry*; Salmon; Crystallography, X-Ray; Cell Line, Tumor
  7. Sosroseno W, Musa M, Ravichandran M, Ibrahim MF, Bird PS, Seymour GJ
    Eur J Oral Sci, 2008 Feb;116(1):31-6.
    PMID: 18186729 DOI: 10.1111/j.1600-0722.2007.00501.x
    Animal studies suggest that inducible nitric oxide synthase (iNOS) may be associated with destructive periodontal disease. l-N(6)-(1-Iminoethyl)-lysine (L-NIL) has been shown to inhibit iNOS in a selective manner, and hence the aim of the present study was to test the hypothesis that treatment with l-NIL may induce a T-cell helper 1 (Th1)-like immune response by Aggregatibacter (Actinobacillus) actinomycetemcomitans lipopolysaccharide (LPS)-stimulated murine spleen cells in vitro. BALB/c mice were either sham-immunized or immunized with A. actinomycetemcomitans LPS. Spleen cells were stimulated with A. actinomycetemcomitans LPS in the presence or absence of L-NIL. Nitric oxide (NO), iNOS activity, specific IgG subclass antibodies, interferon-gamma (IFN-gamma), and interleukin-4 (IL-4) levels and cell proliferation were determined. The results showed that treatment with L-NIL suppressed both NO production and iNOS activity but enhanced specific IgG2a, IFN-gamma levels, and increased cell proliferation following stimulation with A. actinomycetemcomitans LPS-stimulated cells. The results of the present study suggest that inhibition of iNOS activity by L-NIL may skew the A. actinomycetemcomitans LPS-stimulated murine splenic immune response towards the Th1-like immune profile in vitro.
    MeSH terms: Animals; Cells, Cultured/drug effects; Enzyme Inhibitors/pharmacology*; Female; Immunoglobulin G/analysis; Interferon-gamma/analysis; Lysine/analogs & derivatives*; Lysine/pharmacology; Mice, Inbred BALB C; Spleen/cytology; Spleen/immunology*; Interleukin-4/analysis; Aggregatibacter actinomycetemcomitans/enzymology; Aggregatibacter actinomycetemcomitans/immunology*; Cell Proliferation/drug effects; Mice; Nitric Oxide Synthase Type II/analysis; Nitric Oxide Synthase Type II/antagonists & inhibitors*
  8. Shahid SK
    Ann Trop Med Parasitol, 2008 Jan;102(1):63-71.
    PMID: 18186979 DOI: 10.1179/136485908X252151
    Multidrug-resistant organisms cause late-onset ventilator-associated pneumonia (VAP). In a pilot, randomized and controlled study, the efficacy and safety of cefepime, in late-onset VAP in infants, have now been evaluated in Malaysia. Thirty children aged <1 year with late-onset VAP (i.e. VAP occurring 5 or more days after intubation) were randomized to receive cefepime or, as a control, ceftazidime. The clinical responses and the microbiological clearance of tracheal aspirates were evaluated in each arm. Adverse events, if any, were monitored clinically and by blood tests. Ten of the 15 children given cefepime and five of the 15 given ceftazidime showed a satisfactory clinical response (P<0.1). Cefepime appeared significantly better at clearing polymicrobial infections from tracheal aspirates. There were no fatalities in the cefepime arm but three in ceftazidime (P<0.1). The mean (S.E.) durations of antibiotic use were 9.4 (1.5) days for cefepime and 7.6 (1.0) days for ceftazidime (P>0.05). No serious adverse effects were observed in either arm. In conclusion, in late-onset VAP in infants, cefepime monotherapy appears to be at least as effective and safe as ceftazidime monotherapy, with better microbiological clearance.
    MeSH terms: Anti-Bacterial Agents/adverse effects; Anti-Bacterial Agents/therapeutic use*; Ceftazidime/adverse effects; Ceftazidime/therapeutic use*; Cephalosporins/adverse effects; Cephalosporins/therapeutic use*; Child, Preschool; Drug Resistance, Microbial; Female; Humans; Infant; Malaysia; Male; Microbial Sensitivity Tests; Pilot Projects; Statistics as Topic; Pneumonia, Ventilator-Associated/drug therapy*
  9. Lee SW, Liong ML, Yuen KH, Leong WS, Chee C, Cheah PY, et al.
    Am J Med, 2008 Jan;121(1):79.e1-7.
    PMID: 18187077 DOI: 10.1016/j.amjmed.2007.07.033
    Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) afflicts 2%-10% of adult men. Available therapies offer little or no proven benefit. Because acupuncture represents an attractive "natural" therapy, we compared the efficacy of acupuncture to sham acupuncture for CP/CPPS.
    MeSH terms: Adult; Chronic Disease; Double-Blind Method; Follow-Up Studies; Humans; Male; Prostatitis/complications; Prostatitis/diagnosis; Prostatitis/therapy*; Retrospective Studies; Time Factors; Acupuncture Therapy/methods*; Treatment Outcome; Pelvic Pain/diagnosis; Pelvic Pain/etiology; Pelvic Pain/therapy*
  10. Chan YY, Abd Nasir MH, Yahaya MA, Salleh NM, Md Dan AD, Musa AM, et al.
    Int J Food Microbiol, 2008 Feb 29;122(1-2):221-6.
    PMID: 18187222 DOI: 10.1016/j.ijfoodmicro.2007.11.063
    A total of 225 samples from poultry farms and the surrounding environment were screened for vancomycin-resistant enterococci (VRE) and bifunctional aminoglycoside-resistant enterococci using conventional microbiological tests and a nanoplex polymerase chain reaction (PCR) assay. Three (1.3%) of the samples were found to contain vancomycin-resistant isolates (MIC>256 microg/mL) that had a vanA genotype. The three vanA positive VRE isolates were identified as different species. Only one isolate (Enterococcus faecium F 4/13_54) was sensitive to teicoplanin (MIC<0. 12-0.35 microg/mL); the other two VRE (E. faecalis A 21_35 and E. gallinarum F 5/10_1) were resistant to teicoplanin (MIC 3.6-->16 microg/mL). The vanC genotype was observed in nine (4%) of the samples collected. High-level gentamicin-resistant (HLGR) enterococci (with MIC ranging between 100 and 500 microg/mL) were detected in 44 samples. However, only 40 of these were found to possess the aac(6')-aph(2'') gene. The overall prevalence of VRE among the samples from the poultry farms and environment was 5.3%, but the prevalence of the clinically significant vanA VRE was 1.3%, and the prevalence of bifunctional aminoglycoside-resistant enterococci was slightly higher, at 19.5%.
    MeSH terms: Aminoglycosides/pharmacology*; Animals; Anti-Bacterial Agents/pharmacology*; Consumer Product Safety; Dose-Response Relationship, Drug; Feces/microbiology; Food Contamination/analysis; Food Contamination/prevention & control; Genotype; Malaysia; Microbial Sensitivity Tests; Poultry/microbiology*; Poultry Products/microbiology; Colony Count, Microbial; Polymerase Chain Reaction/methods; Enterococcus/drug effects; Enterococcus/isolation & purification*; Vancomycin Resistance*; Drug Resistance, Bacterial
  11. Barnett T, Li Yoong T, Pinikahana J, Si-Yen T
    J Adv Nurs, 2008 Feb;61(3):300-6.
    PMID: 18197864 DOI: 10.1111/j.1365-2648.2007.04528.x
    AIM: This paper is a report of a study to examine the effectiveness of a patient education programme on fluid compliance as assessed by interdialytic weight gain, mean predialysis blood pressure and rate of fluid adherence.
    BACKGROUND: Patients with end stage renal disease who receive haemodialysis are often non-compliant with their treatment regime, especially adherence to fluid restrictions.
    METHOD: An exploratory study was conducted in 2004-05 using a quasi-experimental, single group design to examine the effectiveness of patient education on fluid compliance in a dialysis centre located in a major teaching hospital in Kuala Lumpur, Malaysia. Twenty-six patients with an interdialytic weight gain of greater than 2.5 kg were identified as non-compliant and recruited to the study. The intervention was carried out over a 2-month period and included teaching and weekly reinforcement about diet, fluids and control of weight gain.
    FINDINGS: Patients' mean interdialytic weight gain decreased following the educational intervention from 2.64 kg to 2.21 kg (P < 0.05) and adherence to fluid restrictions increased from 47% to 71% following the intervention. Predialysis mean blood pressure did not improve following the intervention, although the maximum recording for predialysis systolic pressure dropped from 220 mmHg to 161 mmHg. Whilst no statistically significant associations were detected between interdialytic weight gain and age, educational level, marital status or employment status, women demonstrated a greater decrease in mean interdialytic weight gain than men.
    CONCLUSION: Nephrology nurses often have long-term relationships with their patients and are ideally placed to provide ongoing education and encouragement, especially for those experiencing difficulties in adhering to fluid and dietary restrictions.
    MeSH terms: Drug Administration Routes; Female; Renal Dialysis/methods; Renal Dialysis/nursing*; Humans; Kidney Failure, Chronic/nursing*; Health Knowledge, Attitudes, Practice*; Malaysia; Male; Middle Aged; Nurse-Patient Relations/ethics; Patient Compliance; Patient Education as Topic/methods*; Water-Electrolyte Balance; Program Evaluation; Weight Gain; Treatment Outcome; Self Efficacy
  12. Yvonne-Tee GB, Rasool AH, Halim AS, Wong AR, Rahman AR
    Clin. Hemorheol. Microcirc., 2008;38(2):119-33.
    PMID: 18198413
    Recent development had allowed non-invasive assessment of microvascular function in vivo; however, the method has not been fully optimized and standardized. In this study, we aimed to characterize the "effective" occlusion duration needed to elicit sufficient postocclusive hyperemia (PORH) responses in forearm skin using laser Doppler fluximetry (LDF), in subjects with differing age, gender and menstrual phases.
    MeSH terms: Adult; Age Factors; Erythema/etiology; Estrogens/metabolism; Female; Humans; Hyperemia/diagnosis*; Hyperemia/pathology*; Male; Microcirculation*; Middle Aged; Purpura/etiology; Research Design; Sex Factors; Time Factors; Treatment Outcome
  13. Yip WK, Leong VC, Abdullah MA, Yusoff S, Seow HF
    Oncol Rep, 2008 Feb;19(2):319-28.
    PMID: 18202777
    The Akt pathway is one of the most common molecular alterations in various human malignancies. However, its involvement in nasopharyngeal carcinoma (NPC) tumorigenesis has not been well established. In this study, the status of Akt activation and expression of its upstream and downstream molecules was investigated in 64 NPC and 38 non-malignant nasopharyngeal tissues by immunohistochemistry. The hotspot mutations of PIK3CA, encoding the p110alpha catalytic subunit of phosphatidylinositol 3-kinase (PI3K), were also determined in 25 of these NPC tissues. No hotspot mutations were found in any of the samples tested. Akt was activated in 27 (42.2%) and 23 (35.9%) NPCs, as indicated by p-Akt (Thr308) and p-Akt (Ser473) immunoreactivity, respectively. PTEN loss did not correlate statistically with activated Akt. However, a positive correlation was observed between activated Akt and phospho-epidermal growth factor receptor (p-EGFR), suggesting that the EGFR signaling might be one of the upstream regulators of the Akt pathway. The phosphorylation of forkhead (FKHR) and Bcl-2 associated death domain (BAD), but not mammalian target of rapamycin and glycogen synthase kinase-3beta, was significantly correlated with Akt activation. This implies that Akt promotes cell proliferation (as estimated by Ki-67) and survival, at least, through the inactivation of FKHR and BAD in NPC. Our data revealed that the EGFR/PI3K/Akt signaling pathway is important in NPC pathogenesis and that PIK3CA hotspot mutations are rare in NPC.
    MeSH terms: Carcinoma/enzymology*; Carcinoma/genetics; Carcinoma/pathology; Female; Humans; Male; Mutation; Nasopharyngeal Neoplasms/enzymology*; Nasopharyngeal Neoplasms/genetics; Nasopharyngeal Neoplasms/pathology; Phosphorylation; Receptor, Epidermal Growth Factor/analysis; Receptor, Epidermal Growth Factor/metabolism*; Phosphatidylinositol 3-Kinases/genetics; Phosphatidylinositol 3-Kinases/metabolism; bcl-Associated Death Protein/analysis; bcl-Associated Death Protein/metabolism*; Proto-Oncogene Proteins c-akt/analysis; Proto-Oncogene Proteins c-akt/metabolism*; PTEN Phosphohydrolase/metabolism; Forkhead Transcription Factors/analysis; Forkhead Transcription Factors/metabolism*
  14. Qua CS, Wong CH, Goh KL
    Singapore Med J, 2008 Jan;49(1):e8-11.
    PMID: 18204759
    We report a hepatocellular carcinoma seeding following needle biopsy in a 57-year-old man who first presented with a focal hepatic lesion at another hospital. The patient had been a hepatitis B carrier for 20 years. Initial surveillance ultrasonography showed a lesion in segment IV, in the background of non-cirrhotic liver and normal serum alpha-foetoprotein level (8 ng/ml). A percutaneous needle biopsy was done and histopathology confirmed well-differentiated hepatocellular carcinoma. The patient had refused surgery and preferred to try traditional medicine. He presented to us four months after the initial biopsy with epigastric swelling. This was found to be due to a tumour seeding along the previous biopsy tract. He was given radiofrequency ablation (RFA) of tumour along the needle tract, in addition to the primary tumour. We conclude that the need for percutaneous needle biopsy should be critically evaluated in patients presenting with focal hepatic lesions and the role of RFA in treating tumour seeding needs further evaluation.
    MeSH terms: Biopsy; Biopsy, Needle/adverse effects*; Biopsy, Needle/methods*; Carcinoma, Hepatocellular/etiology*; Humans; Liver Neoplasms/etiology*; Male; Middle Aged; Neoplasm Seeding*; Tomography, X-Ray Computed; Ultrasonography; Radiography, Interventional
  15. Hamidah A, Rizal AM, Nordiah AJ, Jamal R
    Singapore Med J, 2008 Jan;49(1):26-30.
    PMID: 18204765
    We evaluated piperacillin-tazobactam in association with amikacin in the initial empirical therapy of febrile neutropenic children.
    MeSH terms: Adolescent; Adult; Amikacin/administration & dosage*; Anti-Bacterial Agents/administration & dosage; Bacterial Infections/drug therapy; Child; Child, Preschool; Drug Therapy, Combination*; Female; Humans; Infant; Male; Neoplasms/complications*; Neoplasms/drug therapy; Neutropenia/drug therapy*; Penicillanic Acid/administration & dosage; Penicillanic Acid/analogs & derivatives; Piperacillin/administration & dosage
  16. Norina TJ, Raihan S, Bakiah S, Ezanee M, Liza-Sharmini AT, Wan Hazzabah WH
    Singapore Med J, 2008 Jan;49(1):67-71.
    PMID: 18204773
    Corneal ulceration remains one of the major causes of blindness in developing countries, including Malaysia. Our objective is to determine the epidemiological characteristics, clinical features, risk factors and the aetiology of microbial keratitis in patients admitted to Hospital Universiti Sains Malaysia (HUSM).
    MeSH terms: Adult; Aspergillus/metabolism; Female; Fusarium/metabolism; Humans; Keratitis/diagnosis*; Keratitis/etiology*; Keratitis/microbiology; Malaysia; Male; Middle Aged; Pseudomonas aeruginosa/metabolism; Pseudomonas Infections/complications; Risk Factors; Streptococcus pneumoniae/metabolism; Eye Infections, Bacterial/diagnosis*; Eye Infections, Fungal/diagnosis*
  17. Elamin EI, Ibrahim MI, Sulaiman SA, Muttalif AR
    Pharm World Sci, 2008 Jun;30(3):281-6.
    PMID: 18204974 DOI: 10.1007/s11096-007-9185-0
    OBJECTIVE: To assess the costs incurred by the public health services and patients as a result of tuberculosis (TB) treatment. Setting The study was conducted in a government hospital located in the northern region of Malaysia.

    METHOD: Retrospective data were collected from medical records and the patients were observed until the completion of their medication. A pharmacoeconomic evaluation was applied to calculate direct and indirect costs.

    MAIN OUTCOME MEASURE: Direct and indirect costs of tuberculosis treatment in a government health institution.

    RESULTS: Two hundred and one tuberculosis patients were included in the study. Different regimens with various durations of treatments were used. The direct medical and non-medical costs as well as indirect costs were calculated and were found to be as follows: US$61.44 for anti-tuberculosis drugs and supplies, US$28.63 for X-ray examinations, US$28.53 for laboratory tests, US$20.03 for healthcare staff time, US$4.28 for hospitalisation, US$43.20 for overhead costs, US$608.11 for transportation and meals and US$118.78 for time away from work. The cost to the patients constitutes approximately 80% of the total cost of the treatment.

    CONCLUSION: The cost of treating the illness of tuberculosis per patient was US$916.4. The cost of anti-tuberculosis drugs constituted the highest proportion of the cost to the public health services (31.7%) while the cost to the patient constituted the major proportion of the total cost of the illness (79.4%).

    MeSH terms: Adolescent; Adult; Aged; Antitubercular Agents/economics; Antitubercular Agents/therapeutic use; Cost-Benefit Analysis; Data Interpretation, Statistical; Ethnic Groups; Female; Humans; Malaysia/epidemiology; Male; Middle Aged; National Health Programs/economics; Tuberculosis/economics*; Tuberculosis/epidemiology; Drug Costs; Cost of Illness*; Economics, Pharmaceutical
  18. Venkatesh G, Majid MI, Ramanathan S, Mansor SM, Nair NK, Croft SL, et al.
    Biomed Chromatogr, 2008 May;22(5):535-41.
    PMID: 18205140 DOI: 10.1002/bmc.965
    A simple, sensitive and specific reversed-phase high-performance liquid chromatographic method with UV detection at 251 nm was developed for quantitation of buparvaquone (BPQ) in human and rabbit plasma. The method utilizes 250 microL of plasma and sample preparation involves protein precipitation followed by solid-phase extraction. The method was validated on a C18 column with mobile phase consisting of ammonium acetate buffer (0.02 m, pH 3.0) and acetonitrile in the ratio of 18:82 (v/v) at a flow rate of 1.1 mL/min. The calibration curves were linear (correlation coefficient>or=0.998) in the selected range. The method is specific and sensitive with limit of quantitation of 50 ng/mL for BPQ. The validated method was found to be accurate and precise in the working calibration range. Stability studies were carried out at different storage conditions and BPQ was found to be stable. Partial validation studies were carried out using rabbit plasma and intra- and inter-day precision and accuracy were within 7%. This method is simple, reliable and can be routinely used for preclinical pharmacokinetic studies for BPQ.
    MeSH terms: Animals; Antiprotozoal Agents/blood; Antiprotozoal Agents/pharmacokinetics; Antiprotozoal Agents/chemistry; Chromatography, High Pressure Liquid/methods*; Humans; Naphthoquinones/blood*; Naphthoquinones/pharmacokinetics*; Naphthoquinones/chemistry; Rabbits; Spectrophotometry, Ultraviolet/methods*; Reproducibility of Results; Molecular Structure; Solid Phase Extraction/methods*
  19. Murty OP
    J Forensic Leg Med, 2008 Feb;15(2):96-100.
    PMID: 18206825 DOI: 10.1016/j.jflm.2007.01.005
    In this article, five cases with pearls in penile skin are discussed. These cases were viewed from multiple angles as number, colour, position of pearls, socio-economic status, occupation, sexual behaviour, and the background of the individuals. The number of pearls varied from one to five in these cases. The penile skin examination revealed old healed scar marks indicating that probably some had even more pearls. Three persons were directly or indirectly associated with drugs. One of the victim was HIV positive. Two of the cases were of homicide; one died in police custody; one died due to ischemic heart disease; and one had died in accident. The article also discusses the common beliefs behind this practice with review of the literature.
    MeSH terms: Adult; Coitus; Culture; Humans; Male; Penis*; Body Modification, Non-Therapeutic*; Forensic Pathology
  20. Thai AC, Mohan V, Khalid BA, Cockram CS, Pan CY, Zimmet P, et al.
    Diabetes Res Clin Pract, 2008 May;80(2):224-30.
    PMID: 18207602 DOI: 10.1016/j.diabres.2007.12.003
    In this paper, the islet autoimmunity status and relation to clinical characteristics, beta cell function and cardio-metabolic risk factors in young-onset Asian diabetic patients are evaluated at baseline. The study population consisted of 912 patients (from China, India, Malaysia and Singapore) with age 12-40 years and diabetes duration <12 months. Autoantibodies to glutamic acid decarboxylase (GADA) and tyrosine phosphatase (IA-2A), beta cell function and cardio-metabolic risk parameters were assessed. Among our young patient cohort, 105 (11.5%) patients were GADA and/or IA-2A positives (Ab +ve). Ab +ve patients were younger, leaner, had more severe hyperglycaemia and lower beta cell function. The frequency of metabolic syndrome was significantly lower in Ab +ve patients (27%) compared to Ab -ve patients (54%). However, a substantial proportion of patients in both groups of patients had atherogenic dyslipidaemia, hypertension and albuminuria (micro or macro). In our study cohort, only one in 10 Asian youth with new-onset diabetes had evidence of islet autoimmunity. At least 60% of Ab +ve and 50% of Ab -ve patients demonstrated classical features of type 1 and type 2 diabetes respectively. Regardless of autoimmunity status, the cardio-metabolic risk factors, in particular atherogenic dyslipidaemia, hypertension and albuminuria were common in our patients with young-onset diabetes.
    MeSH terms: Adolescent; Adult; Australia; Child; Diabetes Mellitus, Type 2/immunology*; Female; Glutamate Decarboxylase/immunology; Humans; Islets of Langerhans/enzymology; Islets of Langerhans/immunology*; Male; Risk Factors; Autoimmunity*; Protein Tyrosine Phosphatases/immunology; Age of Onset; Asian Continental Ancestry Group/ethnology
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