Browse publications by year: 2008

  1. Subramanian R, Asmawi MZ, Sadikun A
    Acta Biochim. Pol., 2008;55(2):391-8.
    PMID: 18511986
    There has been an enormous interest in the development of alternative medicines for type 2 diabetes, specifically screening for phytochemicals with the ability to delay or prevent glucose absorption. The goal of the present study was to provide in vitro evidence for potential inhibition of alpha-glucosidase and alpha-amylase enzymes, followed by a confirmatory in vivo study on rats to generate a stronger biochemical rationale for further studies on the ethanolic extract of Andrographis paniculata and andrographolide. The extract showed appreciable alpha-glucosidase inhibitory effect in a concentration-dependent manner (IC(50)=17.2+/-0.15 mg/ml) and a weak alpha-amylase inhibitory activity (IC(50)=50.9+/-0.17 mg/ml). Andrographolide demonstrated a similar (IC(50)=11.0+/-0.28 mg/ml) alpha-glucosidase and alpha-amylase inhibitory activity (IC(50)=11.3+/-0.29 mg/ml). The positive in vitro enzyme inhibition tests paved way for confirmatory in vivo studies. The in vivo studies demonstrated that A. paniculata extract significantly (P<0.05) reduced peak blood glucose and area under curve in diabetic rats when challenged with oral administration of starch and sucrose. Further, andrographolide also caused a significant (P<0.05) reduction in peak blood glucose and area under the curve in diabetic rats. Hence alpha-glucosidase inhibition may possibly be one of the mechanisms for the A. paniculata extract to exert antidiabetic activity and indicates that AP extract can be considered as a potential candidate for the management of type 2 diabetes mellitus.
    MeSH terms: alpha-Amylases/antagonists & inhibitors*; Animals; Blood Glucose/metabolism; Diabetes Mellitus, Experimental/blood; Diabetes Mellitus, Experimental/drug therapy; Diabetes Mellitus, Type 2/drug therapy; Diterpenes/pharmacology*; Drug Evaluation, Preclinical; Enzyme Inhibitors/pharmacology; Female; Glucose Tolerance Test; Hypoglycemic Agents/pharmacology; Phytotherapy; Plant Extracts/pharmacology; Starch/administration & dosage; Rats, Sprague-Dawley; Andrographis/chemistry*; Rats; Glycoside Hydrolase Inhibitors*; In Vitro Techniques
  2. Razali SM, Mohd Yasin MA
    Epilepsy Behav, 2008 Aug;13(2):343-9.
    PMID: 18514034 DOI: 10.1016/j.yebeh.2008.04.009
    The objective of this study was to describe and compare the pathways followed by Malay patients with psychoses (schizophrenia and schizophreniform disorder) and Malay patients with epilepsy to a tertiary health center in the northeastern area of peninsular Malaysia. There were 60 patients in each group. The most popular pathway for both groups was first contact with traditional or alternative healers. Consultation with Malay traditional healers (bomohs) and/or homeopathic practitioners (44.2%) was significantly higher for psychotic patients (61.7%) than for patients with epilepsy (26.7%) (chi(2)(2)=15.609, P<0.001). Direct access (24.2%) was the second most popular pathway and almost equally followed by both groups of patients. The third and last pathway was initial contact with private general practitioners and government doctors, respectively. Patients with epilepsy dominated the last two pathways. The treatment delay (TD) was significantly longer in epileptic than psychotic patients regardless of their visit to a bomoh and/or homeopathic practitioner (P<0001) or not (p<0.01). The socioeconomic status of psychotic patients also was significantly better than people with epilepsy (chi(2)=9.957, chi(2)(4), p=0.041).

    Study site: Psychiatric clinic, Hospital Universiti Sains Malaysia HUSM
    MeSH terms: Adolescent; Adult; Complementary Therapies/utilization; Cross-Sectional Studies; Developing Countries*; Epilepsy/diagnosis; Epilepsy/epidemiology*; Epilepsy/therapy; Family Practice/statistics & numerical data; Female; Health Services Accessibility/statistics & numerical data; Health Services, Indigenous/utilization; Homeopathy/statistics & numerical data; Hospitals, University; Humans; Malaysia; Male; Middle Aged; Outpatient Clinics, Hospital; Patient Acceptance of Health Care/statistics & numerical data*; Primary Health Care/utilization; Psychotic Disorders/diagnosis; Psychotic Disorders/epidemiology*; Psychotic Disorders/therapy; Referral and Consultation/utilization; Schizophrenia/diagnosis; Schizophrenia/epidemiology*; Socioeconomic Factors
  3. Fakurazi S, Hairuszah I, Nanthini U
    Food Chem Toxicol, 2008 Aug;46(8):2611-5.
    PMID: 18514995 DOI: 10.1016/j.fct.2008.04.018
    Initiation of acetaminophen (APAP) toxicities is believed to be promoted by oxidative stress during the event of overdosage. The aim of the present study was to evaluate the hepatoprotective action of Moringa oleifera Lam (MO), an Asian plant of high medicinal value, against a single high dose of APAP. Groups of five male Sprague-Dawley rats were pre-administered with MO (200 and 800 mg/kg) prior to a single dose of APAP (3g/kg body weight; p.o). Silymarin was used as an established hepatoprotective drug against APAP induced liver injury. The hepatoprotective activity of MO extract was observed following significant histopathological analysis and reduction of the level of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in groups pretreated with MO compared to those treated with APAP alone. Meanwhile, the level of glutathione (GSH) was found to be restored in MO-treated animals compared to the groups treated with APAP alone. These observations were comparable to the group pretreated with silymarin prior to APAP administration. Group that was treated with APAP alone exhibited high level of transaminases and ALP activities besides reduction in the GSH level. The histological hepatocellular deterioration was also evidenced. The results from the present study suggested that the leaves of MO can prevent hepatic injuries from APAP induced through preventing the decline of glutathione level.
    MeSH terms: Acetaminophen/toxicity*; Alanine Transaminase/blood; Alkaline Phosphatase/blood; Animals; Aspartate Aminotransferases/blood; Glutathione/metabolism*; Liver/pathology; Liver Function Tests; Male; Oxidation-Reduction; Plant Extracts/pharmacology; Silymarin/pharmacology; Rats, Sprague-Dawley; Plant Leaves/chemistry; Analgesics, Non-Narcotic/toxicity*; Protective Agents*; Moringa oleifera/chemistry*; Rats; Drug-Induced Liver Injury/metabolism*; Drug-Induced Liver Injury/pathology; Drug-Induced Liver Injury/prevention & control*
  4. Srijit D, Shipra P
    Rom J Morphol Embryol, 2008;49(2):257-8.
    PMID: 18516336
    The present study examines the gross anatomical features of anomalous lunate sulcus detected incidentally in a cadaveric brain and discusses its clinical importance. The absence of lunate sulcus was carefully studied in a dissected brain specimen. The absence of lunate sulcus was observed unilaterally on the right side of a cadaveric brain specimen. The lunate sulcus was clearly appreciated on the left side whilst on the right side it was absent. The right hemisphere of the cerebellum was also bigger in size as compared to the left. The absence of lunate sulcus is a rare finding, which may be detected incidentally. The anatomical knowledge of the lunate sulcus may be important for neurosurgeons operating on the occipital lobe and the radiologists interpreting CT scan.
    MeSH terms: Brain Diseases/diagnosis; Brain Diseases/pathology; Cadaver; Diagnosis; Humans; Male; Middle Aged; Occipital Lobe/abnormalities*; Occipital Lobe/pathology
  5. Murty OP
    Am J Forensic Med Pathol, 2008 Jun;29(2):167-9.
    PMID: 18520487 DOI: 10.1097/PAF.0b013e318177ea88
    Electrocution is one of the rarest modes of suicide. In this case, one school going adolescent committed suicide by electrocution using bare electric wire. This is a rare case of suicidal death by applying live wires around the wrists, simulating the act of judicial electrocution. He positioned himself on armed chair and placed the nude wire loops from a cable around both wrists and switched on the current by plugging in to nearest socket by foot. There were linear electric contact wounds completely encircling around the both wrists. In addition to these linear electric burns all around wrists, there were electrical burns over both hands. This death highlights the need of supervision and close watch on children for self-destructing activities and behavior. This case also highlights unusual method adopted by adolescent to end his life.
    MeSH terms: Adolescent; Capital Punishment; Electric Injuries/pathology*; Hand Injuries/pathology; Humans; Male; Suicide*; Wrist Injuries/pathology; Forensic Pathology
  6. Ong KC, Badmanathan M, Devi S, Leong KL, Cardosa MJ, Wong KT
    J. Neuropathol. Exp. Neurol., 2008 Jun;67(6):532-42.
    PMID: 18520772 DOI: 10.1097/NEN.0b013e31817713e7
    We describe a model of Enterovirus 71 encephalomyelitis in 2-week-old mice that shares many features with the human central nervous system (CNS) disease. Mice were infected via oral and parenteral routes with a murine-adapted virus strain originally from a fatal human case. The mice succumbed to infection after 2 to 5 days. Vacuolated and normal-appearing CNS neurons showed viral RNA and antigens and virions by in situ hybridization, immunohistochemistry, and electron microscopy; inflammation was minimal. The most numerous infected neurons were in anterior horns, motor trigeminal nuclei, and brainstem reticular formation; fewer neurons in the red nucleus, lateral cerebellar nucleus, other cranial nerve nuclei, motor cortex, hypothalamus, and thalamus were infected. Other CNS regions, dorsal root, and autonomic ganglia were spared. Intramuscular-inoculated mice killed 24 to 36 hours postinfection had viral RNA and antigens in ipsilateral lumbar anterior horn cells and adjacent axons. Upper cord motor neurons, brainstem, and contralateral motor cortex neurons were infected from 48-72 hours. Viral RNA and antigens were abundant in skeletal muscle and adjacent tissues but not in other organs. The distinct, stereotypic viral distribution in this model suggests that the virus enters the CNS via peripheral motor nerves after skeletal muscle infection, and spread within the CNS involves motor and other neural pathways. This model may be useful for further studies on pathogenesis and for testing therapies.
    MeSH terms: Animals; Antigens, Viral/analysis; Brain/pathology; Brain/virology; Disease Models, Animal*; Encephalomyelitis/pathology*; Encephalomyelitis/virology*; Enterovirus Infections/pathology*; Immunohistochemistry; Neurons/pathology; Neurons/virology; RNA, Viral/analysis; Spinal Cord/pathology; Spinal Cord/virology; Viremia; In Situ Hybridization; Muscle, Skeletal/virology; Enterovirus A, Human/pathogenicity*; Microscopy, Electron, Transmission; Mice
  7. Leong YK, Xui OC, Chia OK
    J Food Prot, 2008 May;71(5):1035-7.
    PMID: 18522042
    Survival of rotavirus in fresh fruit juices of papaya (Caraca papaya L.), honeydew melon (Cucumis melo L.), and pineapple (Ananas comosus [L.] Merr.) was studied. Clarified juices were prepared from pulps of ripe fruits and sterilized by ultrafiltration. One milliliter of juice from each fruit was inoculated with 20 microl of 1 x 10(6) PFU of SA11 rotavirus and sampled immediately (0-h exposure) and 1 and 3 h later at 28 degrees C. Mean viral titers in juices of papaya (pH 5.1) and honeydew melon (pH 6.3) at 1 and 3 h were not significantly different from titers at 0-h exposure. Mean viral titers in juices from pineapples with ripening color indices of 3 (pH 3.6) and 6 (pH 3.7) at 1-h exposure (color index 3: 4.0 +/- 1.7 x 10(4); color index 6: 2.3 +/- 0.3 x 10(5)) and 3-h exposure (color index 3: 1.1 +/- 0.4 x 10(4); color index 6:1.3 +/- 0.6 x 10(5)) were significantly lower than titers at 0-h exposure (color index 3: 5.7 +/- 2.9 x 10(5); color index 6: 7.4 +/- 1.3 x 10(5)). Virus titers in pineapple juices of color index 3 were significantly lower than titers of the virus in juices of index 6. In cell culture medium (pH 7.4), SA11 titer remained stable over 3 h at 28 degrees C. However, at pH 3.6, the virus titer was reduced to a level not significantly different from that of the virus in pineapple juice of color index 6 (pH 3.7). In conclusion, papaya and honeydew melon juices, in contrast to pineapple juice, have the potential to transmit rotavirus. Inactivation of SA11 virus in pineapple juice can be possibly attributed to low pH and constituent(s) in the juice.
    MeSH terms: Beverages/virology*; Consumer Product Safety; Food Contamination/analysis*; Food Handling/methods*; Food Microbiology; Fruit/virology*; Humans; Hydrogen-Ion Concentration; Rotavirus/growth & development*; Temperature; Time Factors; Ultrafiltration; Colony Count, Microbial; Cucurbitaceae/virology; Carica/virology; Ananas/virology*
  8. Khattak MN, Akhtar S, Mahmud S, Roshan TM
    J Public Health Policy, 2008 Jul;29(2):207-25.
    PMID: 18523475 DOI: 10.1057/jphp.2008.7
    Hepatitis C virus infection is a major health problem worldwide. The current study estimated seroprevalence of Hepatitis C virus (HCV) and evaluated associated factors among volunteer blood donors of the Northwest Frontier Province (NWFP), Pakistan. Of 1,131 volunteer blood donors enrolled, 46 (4.1%) were positive for anti-HCV antibodies. Multivariate logistic regression analysis revealed that positive donors were more likely to be 27-32 years old or >32 years old, have had 1-2 injections or >2 injections in the past year, or 1-5 intravenous (IV) drips or >5 I/V drips in the past 5 years. Positive donors had a family history of jaundice and were more likely to have been shaved (facial and armpit) by barbers. There was high prevalence of anti-HCV antibodies among blood donors of the NWFP. Public awareness programs should target the identified risk factors to prevent HCV transmission. We highlight the weakness of the health care system for blood donation, as it does not offer any record management for donors.
    MeSH terms: Adolescent; Adult; Blood Donors/statistics & numerical data*; Cross-Sectional Studies; Health Education; Hepatitis C/epidemiology*; Humans; Male; Middle Aged; Pakistan/epidemiology; Risk Factors; Socioeconomic Factors; Seroepidemiologic Studies; Age Distribution
  9. Wahab HA, Yam WK, Samian MR, Najimudin N
    J Biomol Struct Dyn, 2008 Aug;26(1):131-46.
    PMID: 18533733
    Macrolides are a group of diverse class of naturally occurring and synthetic antibiotics made of macrocyclic-lactone ring carrying one or more sugar moieties linked to various atoms of the lactone ring. These macrolides selectively bind to a single high affinity site on the prokaryotic 50S ribosomal subunit, making them highly effective towards a wide range of bacterial pathogens. The understanding of binding between macrolides and ribosome serves a good basis in elucidating how they work at the molecular level and these findings would be important in rational drug design. Here, we report refinement of reconstructed PDB structure of erythromycin-ribosome system using molecular dynamics (MD) simulation. Interesting findings were observed in this refinement stage that could improve the understanding of the binding of erythromycin A (ERYA) onto the 50S subunit. The results showed ERYA was highly hydrated and water molecules were found to be important in bridging hydrogen bond at the binding pocket during the simulation time. ERYA binding to ribosome was also strengthened by hydrogen bond network and hydrophobic interactions between the antibiotic and the ribosome. Our MD simulation also demonstrated direct interaction of ERYA with Domains II, V and with C1773 (U1782EC), a residue in Domain IV that has yet been described of its role in ERYA binding. It is hoped that this refinement will serve as a starting model for a further enhancement of our understanding towards the binding of ERYA to ribosome.
    MeSH terms: Anti-Bacterial Agents/metabolism; Anti-Bacterial Agents/chemistry; Computer Simulation; Erythromycin/metabolism*; Erythromycin/chemistry*; Hydrogen Bonding; Models, Chemical; Models, Molecular; Ribosomal Proteins/metabolism; RNA, Ribosomal, 23S/metabolism*; RNA, Ribosomal, 23S/chemistry*; Thermodynamics; Water/chemistry; Ribosome Subunits, Large/metabolism*; Ribosome Subunits, Large/chemistry*
  10. Maha A, Cheong SK, Leong CF, Seow HF
    Hematology, 2008 Feb;13(1):13-20.
    PMID: 18534060 DOI: 10.1179/102453308X315762
    Despite the advances in understanding the pathophysiology of acute myeloid leukaemia (AML), the cure rate for acute myeloid leukaemia patients remains low. Cytogenetic abnormalities and age are the prognostic factors that guide treatment decisions. However, many AML patients still die. The biological factors that influence treatment outcome are largely unknown. Thus, the objective of our study was to use the in vitro viability test to correlate with treatment outcome. Acute myeloid leukaemia blasts demonstrated differing ability to survive in culture. Our examination of blast phenotype at various days in culture showed two possible growth directions. First, cells underwent maturation by increased expression of CD16 and down-regulated CD34 (a haemopoietic stem cell marker). These cells also appeared to have undergone apoptosis. Alternatively, cells continued to survive in culture and maintained high expression of CD34. An MTT assay was carried out to determine viability after three days of culture. Lower optical density values were obtained for samples that underwent apoptosis and higher values were obtained for samples that survived in culture. Apoptosis was measured by Annexin V/propidium iodide staining. A comparison between results of MTT assay and duration of disease free survival revealed that a higher viability in vitro correlated significantly with shorter survival duration in the patient (R -0.761, p=0.002, n=13). Thus, this study further supports the hypothesis that AML patients with poor survival may be related to having blasts with a biologically more immature or stem cell-like nature.
    MeSH terms: Adolescent; Adult; Aged; Cell Differentiation*; Cell Survival; Child; Child, Preschool; Female; Humans; Infant; Male; Middle Aged; Phenotype; Remission Induction; Tumor Cells, Cultured; Neoplastic Stem Cells/pathology*; Cohort Studies; Leukemia, Myeloid, Acute/drug therapy; Leukemia, Myeloid, Acute/pathology*; Survival Analysis; Apoptosis; Granulocyte Precursor Cells/pathology*; Cell Proliferation*; In Vitro Techniques
  11. Lim LH, Gibson DI
    Syst Parasitol, 2008 Jul;70(3):191-213.
    PMID: 18535790 DOI: 10.1007/s11230-008-9137-7
    One new and four previously described species of Triacanthinella Bychowsky & Nagibina, 1968 (Monogenea) were collected from the tripodfishes Triacanthus biaculeatus and Tripodichthys blochii off Peninsular Malaysia. Triacanthinella lumutensis n. sp. from Tripodichthys blochii off Lumut, Selangor is similar to Triacanthinella principalis Bychowsky & Nagibina, 1968 in having morphologically similar types of haptoral sclerites and copulatory organ, but differs in possessing a longer copulatory tube. Also re-described are T. principalis Bychowsky & Nagibina, 1968, T. gracilis Bychowsky & Nagibina, 1968 and T. aspera Bychowsky & Nagibina, 1968 from both Triacanthus biaculeatus and Tripodichthys blochii, plus Triacanthinella longipenis Bychowsky & Nagibina, 1968 from Tripodichthys blochii and Triacanthinella tripathii Bychowsky & Nagibina, 1968 based on its type-material. In the new species, the filament loop of the anchors is associated with a sheath-like sclerite which envelops the anchor point. Such sclerites were also observed in the present specimens of Triacanthinella principalis, T. aspera, T. longipenis and T. gracilis but were not mentioned in the original descriptions. The generic diagnosis of Triacanthinella is amended and a key to the recognised species is presented. The specific names of two of the previously described species are emended from the neuter form to T. principalis and T. gracilis.
    MeSH terms: Animals; Female; Fish Diseases/parasitology; Fishes/parasitology*; Malaysia; Male; Trematoda/anatomy & histology*; Trematoda/classification*; Trematode Infections/parasitology; Trematode Infections/veterinary; Ecosystem
  12. Zulkhairi A, Zaiton Z, Jamaluddin M, Sharida F, Mohd TH, Hasnah B, et al.
    Biomed Pharmacother, 2008 Dec;62(10):716-22.
    PMID: 18538528 DOI: 10.1016/j.biopha.2006.12.003
    There is accumulating data demonstrated hypercholesterolemia and oxidative stress play an important role in the development of atherosclerosis. In the present study, a protective activity of alpha-lipoic acid; a metabolic antioxidant in hypercholesterolemic-induced animals was investigated. Eighteen adult male New Zealand White (NZW) rabbit were segregated into three groups labelled as group K, AT and ALA (n=6). While group K was fed with normal chow and acted as a control, the rest fed with 100 g/head/day with 1% high cholesterol diet to induce hypercholesterolemia. 4.2 mg/body weight of alpha lipoic acid was supplemented daily to the ALA group. Drinking water was given ad-libitum. The study was designed for 10 weeks. Blood sampling was taken from the ear lobe vein at the beginning of the study, week 5 and week 10 and plasma was prepared for lipid profile estimation and microsomal lipid peroxidation index indicated with malondialdehyde (MDA) formation. Animals were sacrificed at the end of the study and the aortas were excised for intimal lesion analysis. The results showed a significant reduction of lipid peroxidation index indicated with low MDA level (p<0.05) in ALA group compared to that of the AT group. The blood total cholesterol (TCHOL) and low density lipoprotein (LDL) levels were found to be significantly low in ALA group compared to that of the AT group (p<0.05). Histomorphometric intimal lesion analysis of the aorta showing less of atheromatous plaque formation in alpha lipoic acid supplemented group (p<0.05) compared to that of AT group. These findings suggested that apart from its antioxidant activity, alpha lipoic acid may also posses a lipid lowering effect indicated with low plasma TCHOL and LDL levels and reduced the athero-lesion formation in rabbits fed a high cholesterol diet.
    MeSH terms: Animals; Hypolipidemic Agents/pharmacology*; Hypolipidemic Agents/therapeutic use; Antioxidants/pharmacology*; Antioxidants/therapeutic use; Aorta/metabolism; Aorta/pathology; Cholesterol/blood; Thioctic Acid/pharmacology*; Thioctic Acid/therapeutic use; Lipoproteins, HDL/blood; Lipoproteins, LDL/blood; Male; Microsomes/metabolism; Rabbits; Triglycerides/blood; Lipid Peroxidation; Tunica Intima/pathology; Atherosclerosis/drug therapy*; Atherosclerosis/metabolism; Atherosclerosis/pathology
  13. B SN, Rodenbaugh DW
    Adv Physiol Educ, 2008 Jun;32(2):169-70.
    PMID: 18539862 DOI: 10.1152/advan.00106.2007
    MeSH terms: Anatomy/education*; Humans; Models, Biological*; Pelvis/anatomy & histology*; Teaching/methods*; Muscle, Skeletal/anatomy & histology
  14. Nyon MP, Rice DW, Berrisford JM, Huang H, Moir AJ, Craven CJ, et al.
    PMID: 18540061 DOI: 10.1107/S1744309108012086
    Cutinase catalyzes the hydrolysis of water-soluble esters and long-chain triglycerides and belongs to the family of serine hydrolases. The enzyme is thought to represent an evolutionary link between the esterase and lipase families and has potential applications in a wide range of industrial hydrolytic processes, for which an understanding of the molecular basis of its substrate specificity is critical. Glomerella cingulata cutinase has been cloned and the protein has been overexpressed in Escherichia coli, purified and subsequently crystallized in a wide range of different crystal forms in the presence and absence of inhibitors. The best crystals are those of the apo cutinase, which diffract to beyond 1.6 A resolution and belong to space group P4(1)2(1)2 or P4(3)2(1)2. Crystals of cutinase with the inhibitors PETFP or E600 belong to space groups P2(1)2(1)2(1) and P2(1), respectively, and diffract to approximately 2.5 A resolution. All of the crystals are suitable for structural studies, which are currently ongoing.
    MeSH terms: Binding Sites; Carboxylic Ester Hydrolases/genetics; Carboxylic Ester Hydrolases/isolation & purification; Carboxylic Ester Hydrolases/chemistry*; Crystallization; Escherichia coli/genetics; Glycine/chemistry; Protein Binding; Recombinant Proteins/chemistry; Serine/chemistry; X-Ray Diffraction; Protein Structure, Secondary; Protein Folding; Phyllachorales/enzymology*
  15. Yu CP, Whynes DK, Sach TH
    Int J Equity Health, 2008 Jun 09;7:15.
    PMID: 18541025 DOI: 10.1186/1475-9276-7-15
    BACKGROUND: Equitable financing is a key objective of health care systems. Its importance is evidenced in policy documents, policy statements, the work of health economists and policy analysts. The conventional categorisations of finance sources for health care are taxation, social health insurance, private health insurance and out-of-pocket payments. There are nonetheless increasing variations in the finance sources used to fund health care. An understanding of the equity implications would help policy makers in achieving equitable financing.

    OBJECTIVE: The primary purpose of this paper was to comprehensively assess the equity of health care financing in Malaysia, which represents a new country context for the quantitative techniques used. The paper evaluated each of the five financing sources (direct taxes, indirect taxes, contributions to Employee Provident Fund and Social Security Organization, private insurance and out-of-pocket payments) independently, and subsequently by combined the financing sources to evaluate the whole financing system.

    METHODS: Cross-sectional analyses were performed on the Household Expenditure Survey Malaysia 1998/99, using Stata statistical software package. In order to assess inequality, progressivity of each finance sources and the whole financing system was measured by Kakwani's progressivity index.

    RESULTS: Results showed that Malaysia's predominantly tax-financed system was slightly progressive with a Kakwani's progressivity index of 0.186. The net progressive effect was produced by four progressive finance sources (in the decreasing order of direct taxes, private insurance premiums, out-of-pocket payments, contributions to EPF and SOCSO) and a regressive finance source (indirect taxes).

    CONCLUSION: Malaysia's two tier health system, of a heavily subsidised public sector and a user charged private sector, has produced a progressive health financing system. The case of Malaysia exemplifies that policy makers can gain an in depth understanding of the equity impact, in order to help shape health financing strategies for the nation.

  16. Lum LC, Suaya JA, Tan LH, Sah BK, Shepard DS
    Am J Trop Med Hyg, 2008 Jun;78(6):862-7.
    PMID: 18541760
    Although the disease burden of dengue is increasing, the impact on the quality of life (QoL) has not been investigated. A study to determine the QoL of confirmed dengue patients using the EuroQol visual thermometer scale was carried out at the University Malaya Medical Center. Of the 207 participants, 40% were ambulatory and 60% were hospitalized. Of eight health domains, 6.2 and 5.0 domains were affected in the hospitalized and ambulatory cohorts, respectively (P < 0.001), with cognition and interpersonal activities affected most. All patients experienced a drastic decrease in their QoL from the onset of symptoms. The QoL deteriorated to the lowest point (40% of healthy status) between the third and seventh days of illness. The duration of impaired QoL (9 days for ambulatory or 13 days for hospitalized patients) was longer than the duration of fever (5 and 7 days, respectively). Symptomatic dengue has major effects on patients' health.

    Study site: e outpatient or inpatient care at the University Malaya Medical
    Center.
    MeSH terms: Adolescent; Adult; Child; Child, Preschool; Dengue/complications; Dengue/epidemiology; Dengue/physiopathology*; Dengue/psychology; Depression/complications; Female; Hospitals, University; Humans; Infant; Infant, Newborn; Malaysia/epidemiology; Male; Middle Aged; Outpatient Clinics, Hospital; Quality of Life*; Cohort Studies
  17. Shirazi MR, Biglary M, Harun SW, Thambiratnam K, Ahmad H
    Appl Opt, 2008 Jun 10;47(17):3088-91.
    PMID: 18545279
    The effects of backward, forward, and bidirectional Raman pumping schemes on stimulated Brillouin scattering (SBS) is investigated in this study. By using a linear cavity, we utilize residual Brillouin pump (BP) and Raman pump (RP) power after each transmission through a 25 km single-mode fiber (SMF) used as a gain medium. The SBS threshold power is reduced in the forward, backward, and bidirectional Raman pumping schemes by 2.5, 1.75, and 2.75 dB, respectively when the 1480 nm RP power is fixed at 150 mW and the BP wavelength is 1580 nm. Surprisingly, it is revealed that the SBS threshold reduction depends strongly and solely on Raman gain and it is independent of the Raman pumping schemes. In addition, the effect of Raman amplification on SBS is more effective at the SBS threshold, especially in the bidirectional and forward schemes.
    MeSH terms: Dental Caries; Dietary Fiber; Laxatives
  18. Al-Mansoori MH, Mahdi MA
    Opt Express, 2008 May 26;16(11):7649-54.
    PMID: 18545472
    We demonstrate an enhanced multiwavelength L-band Brillouin-erbium fiber laser (BEFL), in which the Brillouin pump is pre-amplified before entering the single-mode fiber. The Brillouin pump pre-amplification provided by the Erbium-doped fiber has created higher intensity of Brillouin Stokes line generated in the single-mode fiber that leads to the homogenous gain saturation. Thus the built-up of self-lasing cavity modes is suppressed in a wider wavelength range. In contrary to the conventional linear-cavity BEFL, the number of output channels is enhanced within the same tuning range.
    MeSH terms: Amplifiers, Electronic*; Computer Simulation; Equipment Design; Fiber Optic Technology/instrumentation*; Models, Theoretical*; Computer-Aided Design*; Equipment Failure Analysis; Lasers, Solid-State*
  19. Kim HS, Wu Y, Lin SJ, Deerochanawong C, Zambahari R, Zhao L, et al.
    Curr Med Res Opin, 2008 Jul;24(7):1951-63.
    PMID: 18547466 DOI: 10.1185/03007990802138731
    BACKGROUND: Data on achieving National Cholesterol Education Program Adult Treatment Panel III (ATP III) goals in Asia are limited.

    OBJECTIVE: To examine treatment patterns, goal attainment, and factors influencing treatment among patients in 6 Asian countries who were taking statins.

    METHODS: A retrospective cohort study was conducted in China, Korea, Malaysia, Singapore, Taiwan, and Thailand, where 437 physicians (41% cardiologists) recruited adults with hypercholesterolemia newly initiated on statin monotherapy.

    RESULTS: Of 2622 patients meeting inclusion and exclusion criteria, approximately 66% had coronary heart disease (CHD)/diabetes mellitus, 24% had no CHD but > or =2 risk factors, and 10% had no CHD and <2 risk factors. Most patients ( approximately 90%) received statins at medium or lower equipotency doses. Across all cardiovascular risk categories, 48% of patients attained ATP III targets for low-density lipoprotein cholesterol (LDL-C), including 38% of those with CHD/diabetes (goal: <100 mg/dL), 62% of those without CHD but with > or =2 risk factors (goal: <130 mg/dL), and 81% of those without CHD and <2 risk factors (goal: <160 mg/dL). Most patients who achieved goals did so within the first 3 months. Increasing age (odds ratio (OR)=1.015 per 1-year increment; 95% confidence interval (CI)=1.005-1.206; p=0.0038) and initial statin potency (OR=2.253; 95% CI=1.364-3.722; p=0.0015) were directly associated with goal attainment, whereas increased cardiovascular risk (OR=0.085; 95% CI=0.053-0.134; p<0.0001 for CHD/diabetes mellitus at baseline compared with <2 risk factors,) and baseline LDL-C (OR=0.990; 95% CI=0.987-0.993); p<0.0001 per 1-mg/dL increment) were inversely associated with LDL-C goal achievement. Limitations of this study include potential differences in treatment settings and cardiovascular risk factors between different countries and centers. In addition, the effects on cholesterol goal achievement of concomitant changes in lifestyle were not assessed.

    CONCLUSION: LDL-C goal attainment is low in Asians, particularly those with CHD/diabetes. More effective patient monitoring, treatments, including combining regimens and dose titration, and adherence to these treatments along with therapeutic lifestyle counseling may facilitate goal attainment.

    MeSH terms: Aged; Asia; Coronary Disease/complications; Female; Humans; Hypercholesterolemia/blood; Hypercholesterolemia/drug therapy*; Cholesterol, LDL/blood; Cholesterol, LDL/metabolism*; Male; Middle Aged; Retrospective Studies; Cohort Studies; Treatment Outcome; Hydroxymethylglutaryl-CoA Reductase Inhibitors/administration & dosage*; Hydroxymethylglutaryl-CoA Reductase Inhibitors/adverse effects; Diabetes Complications
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