Browse publications by year: 2008

  1. Nurita AT, Abu Hassan A, Nur Aida H, Norasmah B
    Trop Biomed, 2008 Aug;25(2):126-33.
    PMID: 18948883
    The efficacy and residual efficacy of commercial baits, Quick Bayt (0.5% w/w imidacloprid) and Agita (10.0% w/w thiamethoxam) against synanthropic flies were evaluated under field conditions. Efficacy was evaluated based on knockdown percentage (KD %). The bait efficacy and residual efficacy evaluation were conducted for a period of 3 weeks and 6 weeks respectively. Baits were applied onto bait targets and placed on fly-count targets to facilitate the counting of flies. All baits were applied according to the manufacturer's recommended application rate. Three replicate treatments for each type of bait were placed at the study site each week. The number of flies feeding on baits and the knocked down flies were counted and collected. The efficacy of Agita and Quick Bayt did not differ significantly (t-test, P>0.05) over the 3-week period, even though Quick Bayt had a slightly higher KD% than Agita. In the residual efficacy evaluation, the (knockdown) KD% of Quick Bayt was consistent at around 36% for the first five weeks but dropped to 33.8 +/- 0.4% on the sixth week. The KD% for Agita on the first week was 33.6 +/- 12.2% and remained relatively consistent for the first 4 weeks at around 31%. KD% dropped to 16.7 +/- 3.3% on week 5 and to 15.7 +/- 1.2% on week 6. The difference in residual efficacy of the two baits was significant (t-test, p < 0.05).
    MeSH terms: Animals; Diptera/drug effects*; Insect Control/methods*; Insecticides/pharmacology*; Time Factors
  2. Lee HY, Stephen A, Sushela D, Mala M
    Trop Biomed, 2008 Aug;25(2):134-9.
    PMID: 18948884
    Parasites and bacteria are reported in the faeces of birds in the current study. Fresh faecal samples of the large-billed crow (Corvus spp.) were collected from the study site at Bangsar, an urban setting in Kuala Lumpur, Malaysia. These samples were transported to laboratory and analysed for parasites and bacteria. Pre-prepared XLD agar plates were used for culturing the bacteria in the laboratory. Using the API 20ETM Test Strips, 9 different species of bacteria were identified belonging to the family Enterobacteriacea. They were Citrobacter freundii, Enterobacter cloacae, Proteus mirabilis, Klebsiella pneumoniae, Kluyvera ascorbata, Salmonella arizonae, Salmonella typhi, Shigella flexneri and Shigella sonnei. The protozoan parasites detected include Cryptosporidium spp., Cyclospora spp., Blastocystis spp., and Capillaria hepatica and Ascaris lumbricoidus ova. Environmental air samples collected on agar plates using an air sampler in the area only produced fungal colonies. Some of these pathogens found in the crows are of zoonotic importance, especially Cryptosporidium, Blastocystis, Cyclopsora, Salmonella, Shigella and Kluyvera. The finding of Kluyvera spp. in crows in our current study highlights its zoonotic potential in an urban setting.
    MeSH terms: Animals; Bacteria/classification; Bacteria/isolation & purification*; Feces/microbiology*; Feces/parasitology*; Malaysia; Public Health; Crows/microbiology*; Crows/parasitology*; Eukaryota/classification; Eukaryota/isolation & purification*
  3. Nurita AT, Abu Hassan A, Nur Aida H
    Trop Biomed, 2008 Aug;25(2):145-53.
    PMID: 18948886 MyJurnal
    Three species composition surveys were conducted in a rural location in Kedah and an urban location in Pulau Pinang. Two of the surveys were conducted in November 2003, the first was at the Kedah site and the second was at the Pulau Pinang site. The third survey was conducted at the Pulau Pinang site again on the last week of April 2004. All these surveys were conducted one week prior to field evaluations of commercial chemical fly baits. The predominant species recovered from the surveys was the house fly, Musca domestica, which ranked first in prevalence in all three studies. Catches of Musca sorbens, Chrysoma megacephala and Lucillia cuprina were lower than M. domestica. Sarcophaga sp. was not present at the Kedah site and was only present at the Pulau Pinang site during the survey in April 2004. The other fly species present at the Kedah site were Megaselia sp., Psycoda sp., Piophila sp. and Fannia sp. These species were scarce and never exceeded 1% of the total catch.
    MeSH terms: Animals; Data Collection; Diptera/classification*; Humans; Malaysia; Population Dynamics
  4. Wahab AR, Andy Tan WA, Intan S
    Trop Biomed, 2008 Aug;25(2):160-5.
    PMID: 18948888
    A total of fifty anurans, comprising of Rana limnocharis and Bufo melanostictus were collected from Sungai Pinang, Balik Pulau, Penang. The prevalence, mean intensity and distribution of parasite species along the digestive tract were reported. Seven species of parasites were recorded. Blood parasites recovered were trypansomes and microfilariae.
    MeSH terms: Animals; Bufonidae/parasitology*; Malaysia; Microfilaria; Ranidae/parasitology*; Eukaryota
  5. Thanikachalam MP, Kasemsuk Y, Mak JW, Sharifah Emilia TS, Kandasamy P
    Trop Biomed, 2008 Aug;25(2):166-72.
    PMID: 18948889 MyJurnal
    Appendicitis has a worldwide prevalence and affects all age groups. The aetiology of acute appendicitis is still much debated, many factors have been implicated. The pathology is likely to be due to obstruction of the lumen of the appendix. Parasites, both helminths and protozoa have been suggested to be the cause of acute appendicitis. Studies have demonstrated that parasites are present in the appendix specimens removed from surgery methods.
    MeSH terms: Adolescent; Adult; Aged, 80 and over; Appendix/parasitology*; Child; Child, Preschool; Female; Gastrointestinal Contents/parasitology*; Humans; Male; Protozoan Infections/parasitology; Young Adult
  6. Nazni WA, Jeffery J, Sa'diyah I, Noorjuliana WM, Chen CD, Rohayu SA, et al.
    Trop Biomed, 2008 Aug;25(2):173-5.
    PMID: 18948890
    Piophila casei (Linnaeus) (Diptera: Piophilidae) is reported from human cadavers in two separate forensic cases for the first time in Malaysia. Both bodies were found indoors. The first case, was that of a male of unknown nationality and age and also contained maggots of the muscid Ophyra spinigera (Stein). The second case was a female Chinese whose body also contained other species of maggots but these were not identifiable.
    MeSH terms: Animals; Cadaver*; Diptera/physiology*; Entomology; Female; Forensic Medicine; Humans; Larva; Malaysia; Male
  7. Vasudevan R, Ismail P, Stanslas J, Shamsudin N, Ali AB
    Int J Biol Sci, 2008;4(6):362-7.
    PMID: 18953403
    An insertion/deletion (I/D) polymorphism of Alpha2B-Adrenoceptor (ADRA2B) gene located on chromosome 2 has been studied extensively in related to cardiovascular diseases. The main aim of the present study was to examine the potential association of D allele frequency of I/D polymorphism of ADRA2B gene in Malaysian essential hypertensive subjects with or without type 2 diabetes mellitus (T2DM). This study includes 70 hypertensive subjects without T2DM, 65 hypertensive subjects with T2DM and 75 healthy volunteers as control subjects. Genotyping of I/D polymorphism was performed by conventional PCR method. There was significant difference found in age, body mass index, systolic/diastolic blood pressure and high density lipoprotein cholesterol level between the case and control subjects. DD genotypic frequency of I/D polymorphism was significantly higher in hypertensive subjects (42.84% vs. 29.33%; P-=0.029) and in hypertensive with T2DM subjects (46.15% vs. 29.33%; P=0.046) than control group. D allele frequency was higher in hypertensive group (67.41%) than control subjects (52.67%). However, no significant difference was found between the three genotypes of I/D polymorphism of ADRA2B gene and the clinical characteristics of the subjects. The result obtained in this study show D allele of ADRA2B gene was associated with essential hypertension with or without T2DM in Malaysian subjects.
    MeSH terms: Adult; Aged; Diabetes Mellitus, Type 2/complications; Diabetes Mellitus, Type 2/genetics*; Female; Gene Frequency; Genotype; Humans; Hypertension/complications; Hypertension/genetics*; Malaysia; Male; Middle Aged; Case-Control Studies; Receptors, Adrenergic, alpha-2/genetics*; INDEL Mutation*
  8. Nayak C, Nayak D, Raja A, Rao A
    Neurol Res, 2008 Jun;30(5):461-4.
    PMID: 18953735
    Epidemiologic works reveal that moderate head injury (MHI) is more frequent and a substantial number of these patients develop complications resulting in neurological disabilities. Reactive oxygen species (ROS) play a major role in post-traumatic neuronal damage following traumatic head injury. Thus, the current study analysed the post-traumatic changes in the erythrocyte markers of oxidative damage and the relationship between these parameters and Glasgow coma scale (GCS) scores of MHI patients during the 7 day study period.
    MeSH terms: Adolescent; Adult; Analysis of Variance; Female; Glutathione/metabolism*; Craniocerebral Trauma/metabolism*; Craniocerebral Trauma/physiopathology*; Humans; Male; Middle Aged; Mass Spectrometry/methods; Statistics as Topic; Time Factors; Lipid Peroxidation/physiology*; Glasgow Coma Scale*; Thiobarbituric Acid Reactive Substances/metabolism
  9. Damit EF, Ariffin A, Khan MN
    J Org Chem, 2008 Dec 5;73(23):9303-13.
    PMID: 18954113 DOI: 10.1021/jo8011072
    Apparent second-order rate constants (k(n)(app)) for the nucleophilic reaction of aniline (Ani) with phthalic anhydride (PAn) vary from 6.30 to 7.56 M(-1) s(-1) with the increase of temperature from 30 to 50 degrees C in pure glacial acetic acid (AcOH). However, the values of pseudo-first-order rate constants (k(s)) for the acetolysis of PAn in pure AcOH increase from 16.5 x 10(-4) to 10.7 x 10(-3) s(-1) with the increase of temperature from 30 to 50 degrees C. The values of k(n)(app) and k(s) vary from 5.84 to 7.56 M(-1) s(-1) and from 35.1 x 10(-4) to 12.4 x 10(-4) s(-1), respectively, with the increase of CH(3)CN content from 1% to 80% v/v in mixed AcOH solvents at 35 degrees C. The plot of k(s) versus CH(3)CN content shows a minimum (with 10(4) k(s) = 4.40 s(-1)) at 50% v/v CH(3)CN. Similarly, the variations of k(n)(app) and k(s) with the increasing content of tetrahydrofuran (THF) in mixed AcOH solvent reveal respective a maximum (with k(n)(app) = 17.5-15.6 M(-1) s(-1)) at 40-60% v/v THF and a minimum (with k(s) = approximately 0-1.2 x 10(-4) s (-1)) at 60-70% v/v THF. The respective values of DeltaH* and DeltaS* are 15.3 +/- 1.2 kcal mol(-1) and -20.1 +/- 3.8 cal K(-1) mol(-1) for k(s) and 1.1 +/- 0.5 kcal mol(-1) and -51.2 +/- 1.7 cal K(-1) mol(-1) for k(n)(app), while the values of k(n) (= k(n)(app)/f(b) with f(b) representing the fraction of free aniline base) are almost independent of temperature within the range 30-50 degrees C. A spectrophotometric approach has been described to determine f(b) in AcOH as well as mixed AcOH-CH(3)CN and AcOH-THF solvents. Thus, the observed data, obtained under different reaction conditions, have been explained quantitatively. An optimum reaction condition, within the domain of present reaction conditions, has been suggested for the maximum yield of the desired product, N-phenylphthalamic acid.
  10. Hailemariam Z, Omar AR, Hair-Bejo M, Giap TC
    Virol J, 2008;5:128.
    PMID: 18954433 DOI: 10.1186/1743-422X-5-128
    Chicken anemia virus (CAV) is the causative agent of chicken infectious anemia (CIA). Study on the type of CAV isolates present and their genetic diversity, transmission to their progeny and level of protection afforded in the breeder farms is lacking in Malaysia. Hence, the present study was aimed to detect CAV from commercial broiler breeder farms and characterize CAV positive samples based on sequence and phylogenetic analysis of partial VP1 gene.
    MeSH terms: Animals; Antigens, Viral/isolation & purification; Bone Marrow/virology; Chick Embryo; Chickens; DNA, Viral/genetics; Genotype; Malaysia/epidemiology; Phylogeny; Poultry Diseases/epidemiology; Poultry Diseases/virology*; Thymus Gland/virology; Cluster Analysis; Sequence Homology; Sequence Analysis, DNA; Molecular Epidemiology; Chicken anemia virus/classification*; Chicken anemia virus/genetics; Chicken anemia virus/isolation & purification*; Circoviridae Infections/epidemiology; Circoviridae Infections/veterinary*; Circoviridae Infections/virology
  11. Mallick Z
    Int J Occup Saf Ergon, 2008;14(3):343-9.
    PMID: 18954544
    The grass trimming machine is a widely used agricultural machine for cutting grass by the roadside and in other areas in Malaysia. Hand-arm vibration (HAV) syndrome is very common among workers operating power tools and performing similar work for extended periods. Grass trimming involves the use of a motorized cutter spinning at high speed, resulting in high levels of HAV among its operators. The existing D-shape handle causes HAV-related stress and operational load in operators. This research proposes a new design of a handle of the grass trimming machine. When this new design was compared with the old one, it was found that the new handle resulted in 18% lower HAV. To find the lowest HAV, 3 critical parameters of the new handle (length, angle and material of the cap of the handle) were optimized using the Taguchi quality tool. Appropriately selected parameters of the new handle significantly reduced the occurrence of HAV among grass trimmers.
    MeSH terms: Analysis of Variance; Equipment Design; Poaceae; Humans; Human Engineering; Malaysia/epidemiology; Occupational Diseases/prevention & control*; Hand-Arm Vibration Syndrome/epidemiology; Hand-Arm Vibration Syndrome/prevention & control*
  12. Rahman RA, Ahmad A, Rahman ZA, Mokhtar KI, Lah NA, Zilfalil BA, et al.
    Cleft Palate Craniofac J, 2008 Nov;45(6):583-6.
    PMID: 18956930 DOI: 10.1597/07-020.1
    To determine the frequency of the transforming growth factor-alpha (TGFalpha) Taq1 polymorphism in nonsyndromic cleft lip with or without cleft palate (CL+/-P) and cleft palate only (CP) in Kelantan, Malaysia.
    MeSH terms: Adolescent; Child; Child, Preschool; Cleft Lip/genetics*; Cleft Palate/genetics*; Female; Gene Frequency; Humans; Infant; Malaysia; Male; Polymorphism, Restriction Fragment Length; Case-Control Studies; Transforming Growth Factor alpha/genetics*; Taq Polymerase/genetics; Young Adult
  13. Root R
    Med Anthropol, 2008 Oct-Dec;27(4):405-34.
    PMID: 18958787 DOI: 10.1080/01459740802427737
    Since the early 1990s, the Malaysian government has identified factories as high risk for HIV and AIDS. Signaling epidemiological concerns over the rising rates of HIV among factory workers, a significant proportion of whom are women, the label also appeared to reconstitute stereotypes of factory women as dangerously sexual and of factories as immoral spaces. Drawing on ethnographic research in the export processing zones of Penang, Malaysia in the mid-1990s, I examine the meanings and experiences of HIV risk among factory women themselves. Data were analyzed using discourse and grounded theory methods, the former to identify women's multiple modes of rationalizing HIV risks, and the latter to theorize the sources and significance of women's HIV risk assemblages. The heuristic of assemblages as localized knowledge spaces helped to show that biomedical and socioreligious risk lexica operated not as fixed epistemological categories but as situational resources in women's risk scripts. Overall, women desired multiple risk knowledges to help them "control themselves by themselves," a project of reflexive self-shaping mediated by the diverse and discordant discourses of gender, ethnicity, and modernity in Malaysia that shaped how HIV risks were engendered and experienced.
    MeSH terms: Adolescent; Adult; Anthropology, Cultural; Cultural Characteristics; Female; Humans; Islam; Health Knowledge, Attitudes, Practice*; Malaysia/epidemiology; Male; Surveys and Questionnaires; Risk-Taking; Sexual Behavior/ethnology*; Sexual Behavior/psychology; Women, Working/psychology*; HIV Infections/ethnology*; HIV Infections/psychology*; Workplace/psychology*; Young Adult
  14. Tan HJ, Goh KL
    J Dig Dis, 2008 Nov;9(4):186-9.
    PMID: 18959588 DOI: 10.1111/j.1751-2980.2008.00344.x
    As in developed societies, the prevalence of Helicobacter pylori has declined rapidly in Asia. This has been shown in both seroprevalence-based and endoscopy-based studies. While the decline in the incidence of gastric cancer has now been observed, a decrease in peptic ulcer disease has not been so clearly evident. This apparent paradox can be explained by an increase in non-H. pylori associated ulcers - such as those related to non-steroidal anti-inflammatory drugs or idiopathic ulcers. The increase of gastroesophageal reflux disease in Asia has been widely observed and commented on and its relationship to the decline in H. pylori speculated upon. However there have been few conclusive studies from Asia on this subject. While the improved diagnosis and elimination of H. pylori has contributed to its decline, a more basic change involving large segments of the Asian population must be responsible. An improvement in hygiene and living conditions that results from more affluent Asian societies is thought to be a possible cause.
    MeSH terms: Asia/epidemiology; Gastroesophageal Reflux/epidemiology*; Humans; Peptic Ulcer/epidemiology*; Stomach Neoplasms/epidemiology*; Prevalence; Seroepidemiologic Studies; Endoscopy, Gastrointestinal; Helicobacter pylori*; Helicobacter Infections/epidemiology*
  15. Das S
    ANZ J Surg, 2008 Nov;78(11):939.
    PMID: 18959687 DOI: 10.1111/j.1445-2197.2008.04708.x
    MeSH terms: Health Personnel/statistics & numerical data*; Humans; Latex/adverse effects*; Occupational Diseases/epidemiology*; Global Health; Incidence; Prevalence; Occupational Exposure/adverse effects*; Gloves, Protective/adverse effects*; Latex Hypersensitivity/epidemiology*
  16. Haron NH, Taib NA, Yip CH
    ANZ J Surg, 2008 Nov;78(11):943-4.
    PMID: 18959689 DOI: 10.1111/j.1445-2197.2008.04709.x
    MeSH terms: Axilla; Female; Humans; Lymph Node Excision/methods*; Lymph Nodes/pathology*; Lymphatic Metastasis; Mastectomy; Outcome Assessment (Health Care)*
  17. Domingo EJ, Noviani R, Noor MR, Ngelangel CA, Limpaphayom KK, Thuan TV, et al.
    Vaccine, 2008 Aug 19;26 Suppl 12:M71-9.
    PMID: 18945416 DOI: 10.1016/j.vaccine.2008.05.039
    Cervical cancer remains one of the leading causes of cancers in women from Indonesia, Malaysia, the Philippines, Thailand and Vietnam. High-risk human papillomavirus (HPV) types, particularly HPV-16 and 18, are consistently identified in cervical cancer cases regardless of geographical region. Factors that have been identified to increase the likelihood of HPV exposure or subsequent development of cervical cancer include young age at first intercourse, high parity and multiple sexual partners. Cervical cancer screening programs in these countries include Pap smears, single visit approach utilizing visual inspection with acetic acid followed by cryotherapy, as well as screening with colposcopy. Uptake of screening remains low in all regions and is further compounded by the lack of basic knowledge women have regarding screening as an opportunity for the prevention of cervical cancer. Prophylactic HPV vaccination with the quadrivalent vaccine has already been approved for use in Malaysia, the Philippines and Thailand, while the bivalent vaccine has also been approved in the Philippines. However, there has been no national or government vaccination policy implemented in any of these countries.
    MeSH terms: Adolescent; Adult; Aged; Uterine Cervical Neoplasms/diagnosis; Uterine Cervical Neoplasms/epidemiology*; Uterine Cervical Neoplasms/prevention & control*; Uterine Cervical Neoplasms/virology; Female; Humans; Indonesia/epidemiology; Malaysia/epidemiology; Male; Mass Screening/methods; Middle Aged; Philippines/epidemiology; Risk Factors; Sexual Behavior; Thailand/epidemiology; Vaccination; Vaginal Smears; Vietnam/epidemiology; Prevalence; Papillomavirus Infections/complications; Papillomavirus Infections/diagnosis; Papillomavirus Infections/epidemiology*; Papillomavirus Infections/prevention & control*; Human papillomavirus 18/classification; Human papillomavirus 18/isolation & purification; Human papillomavirus 16/classification; Human papillomavirus 16/isolation & purification; Papillomavirus Vaccines/therapeutic use; Young Adult; Papanicolaou Test
  18. Othman S, Mat Adenan NA
    Asia Pac Fam Med, 2008 Sep 29;7(1):2.
    PMID: 18973706 DOI: 10.1186/1447-056X-7-2
    AIM: To assess the knowledge, attitudes and practices of primary health care providers regarding the identification and management of domestic violence in a hospital based primary health care setting.

    METHOD: A survey of all clinicians and nursing staff of the outpatient, casualty and antenatal clinics in University Malaya Medical Centre using a self-administered questionnaire.

    RESULTS: Hundred and eight out of 188 available staff participated. Sixty-two percent of the clinicians and 66.9% of the nursing staff perceived the prevalence of domestic violence within their patients to be very rare or rare. Majority of the clinicians (68.9%) reported asking their patients regarding domestic violence 'at times' but 26.2% had never asked at all. Time factor, concern about offending the patient and unsure of how to ask were reported as barriers in asking for domestic violence by 66%, 52.5% and 32.8% of the clinicians respectively. Clinicians have different practices and levels of confidence within the management of domestic violence. Victim-blaming attitude exists in 28% of the clinicians and 51.1% of the nursing staff. Less than a third of the participants reported knowing of any written protocol for domestic violence management. Only 20% of the clinicians and 6.8% of the nursing staff had ever attended any educational program related to domestic violence.

    CONCLUSION: Lack of positive attitude and positive practices among the staff towards domestic violence identification and management might be related to inadequate knowledge and inappropriate personal values regarding domestic violence.

  19. Ho WF, Koo SH, Yee JY, Lee JD
    Drug Metab. Pharmacokinet., 2008;23(5):385-91.
    PMID: 18974617
    MRP2 is a drug transporter that is responsible for the gastrointestinal absorption and biliary excretion of a wide variety of endogenous and xenobiotic compounds, including many clinically used drugs. This study aims to identify genetic variations of ABCC2 gene in three distinct ethnic groups of the Singaporean population (n = 288). The coding region of the gene encoding the transporter protein was screened for genetic variations in the study population by denaturing high-performance liquid chromatography and DNA sequencing. Twenty-two genetic variations of ABCC2, including 8 novel ones, were found: 1 in the 5' untranslated region, 10 in the coding exons (8 nonsynonymous and 2 synonymous variations), and 11 in the introns. Three novel nonsynonymous variations: 2686G > A (Glu896Lys), 4240C > T (His1414Tyr) and 4568A > C (Gln1523Pro) were detected in single heterozygous Malay, Chinese, and Indian subjects, respectively. Among the novel nonsynonymous variations, 4240C > T and 4568A > C were predicted to be functionally significant. These data would provide fundamental and useful information for pharmacogenetic studies on drugs that are substrates of MRP2 in Asians.
    MeSH terms: Adolescent; Adult; China/ethnology; Ethnic Groups; Female; Humans; India/ethnology; Malaysia/ethnology; Male; Middle Aged; Singapore; Genetic Variation*; Polymorphism, Single Nucleotide*; Multidrug Resistance-Associated Proteins/genetics*; Asian Continental Ancestry Group/genetics*
  20. Karim AA, Sufha EH, Zaidul IS
    J Agric Food Chem, 2008 Nov 26;56(22):10901-7.
    PMID: 18975963 DOI: 10.1021/jf8015442
    The effect of enzymatic pretreatment on the degree of corn and mung bean starch derivatization by propylene oxide was investigated. The starch was enzymatically treated in the granular state with a mixture of fungal alpha-amylase and glucoamylase at 35 degrees C for 16 h and then chemically modified to produce enzyme-hydrolyzed-hydroxypropyl (HP) starch. Partial enzyme hydrolysis of starch in the granular state appeared to enhance the subsequent hydroxypropylation, as judged from the significant increase in the molar substitution. A variable degree of granule modification was obtained after enzyme hydrolysis, and one of the determinants of the modification degree appeared to be the presence of natural pores in the granules. Enzyme-hydrolyzed-HP starch exhibited significantly different functional properties compared to hydroxypropyl starch prepared from untreated (native) starch. It is evident that the dual modification of starch using this approach provides a range of functional properties that can be customized for specific applications.
    MeSH terms: alpha-Amylases/metabolism*; Amylose/analysis; Aspergillus/enzymology; Zea mays/chemistry; Epoxy Compounds/metabolism; Glucan 1,4-alpha-Glucosidase/metabolism*; Hydrolysis; Hydroxylation; Microscopy, Electron, Scanning; Seeds/chemistry; Starch/metabolism*; Starch/ultrastructure; Starch/chemistry; Phaseolus/chemistry; Physicochemical Phenomena
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