Browse publications by year: 2010

  1. Thong MK, Boey CC, Sheng JS, Ushikai M, Kobayashi K
    Singapore Med J, 2010 Jan;51(1):e12-4.
    PMID: 20200759
    We report two Malaysian siblings with neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD). The younger sibling, a six-month-old Chinese girl, presented with prolonged neonatal jaundice, and was investigated for biliary atresia. Urine metabolic screen showed the presence of urinary-reducing sugars, and she was treated with a lactose-free formula. NICCD was suspected based on the clinical history, examination and presence of urinary citrulline. Mutation study of the SLC25A13 gene showed the compound heterozygotes, 851del4 and IVS16ins3kb, which confirmed the diagnosis of NICCD in the patient and her three-year-old female sibling, who also had unexplained neonatal cholestasis. Long-term dietary advice, medical surveillance and genetic counselling were provided to the family. The diagnosis of NICCD should be considered in infants with unexplained prolonged jaundice. DNA-based genetic testing of the SLC25A13 gene may be performed to confirm the diagnosis retrospectively. An awareness of this condition may help in early diagnosis using appropriate metabolic and biochemical investigations, thus avoiding invasive investigations in infants with neonatal cholestasis caused by NICCD.
    MeSH terms: Calcium-Binding Proteins/deficiency*; Child, Preschool; Cholestasis, Intrahepatic/etiology; Cholestasis, Intrahepatic/genetics*; Cholestasis, Intrahepatic/therapy; Diet Therapy; Female; Genetic Counseling; Humans; Infant; Malaysia; Metabolism, Inborn Errors/diagnosis; Metabolism, Inborn Errors/genetics*; Gene Deletion; Organic Anion Transporters/deficiency*; Mitochondrial Membrane Transport Proteins/genetics*; Siblings
  2. Peh WC, Ng KH
    Singapore Med J, 2010 Jan;51(1):10-3; quiz 14.
    PMID: 20200769
    A case report is a description of a single case with unique features. This includes a previously-unreported clinical condition, previously-unreported observation of a recognised disease, unique use of imaging or diagnostic test to reveal a disease, previously-unreported treatment in a recognised disease, or previously-unreported complication of a procedure. Case reports should be short and focused, with a limited number of figures and references. The structure of a case report usually comprises a short unstructured (or no) abstract, brief (or no) introduction, succinct but comprehensive report of the case, and to-the-point discussion.
    MeSH terms: Humans; Periodicals as Topic*; Editorial Policies
  3. Hasan SS, Bahari MB, Babar ZU, Ganesan V
    Singapore Med J, 2010 Jan;51(1):21-7.
    PMID: 20200771
    INTRODUCTION: The primary purpose of this study was to evaluate the utilisation pattern and seizure outcome of newer and older antiepileptic drugs in paediatric clinical practice in a Malaysian hospital setting.
    METHODS: Over a two-month period, all paediatric epilepsy patients from Penang General Hospital, Malaysia who were diagnosed according to the classification of the International League Against Epilepsy were followed up prospectively, and the patients' information was gathered with the help of a validated data collection form. This included demographic characteristics, monotherapy and polytherapy of antiepileptic drugs, as well as the number of seizures experienced.
    RESULTS: Partial seizures, including complex and simple partial seizures (47.2 percent), followed by generalised seizures (40 percent), were the most common seizure types found in this study. An average of 1.51 antiepileptic drugs per patient was prescribed, with 54.3 percent of the patients on monotherapy and 45.7 percent on polytherapy. Overall, sodium valproate was the most frequently prescribed antiepileptic drug (36.8 percent), followed by carbamazepine (30.2 percent) and lamotrigine (10.4 percent). Carbamazepine was the most frequently prescribed monotherapy (28.6 percent), followed by sodium valproate (17.1 percent). The newer antiepileptic drugs were also found to be used as monotherapy in 7.2 percent of the cases. Moreover, a significant difference was observed between the older and newer antiepileptic drugs in terms of the number of seizures experienced (p=0.027). Most (75 percent) of the seizure-free patients were on carbamazepine monotherapy.
    CONCLUSION: Monotherapy was the most frequently used remedy in all forms of epilepsy. Overall, sodium valproate was the most commonly used drug, while carbamazepine was found to be more frequently used as monotherapy. There was a significant difference found between the older and newer antiepileptic drugs, with 87.5 percent of seizure-free patients on older antiepileptic drugs.

    Study site: all paediatric epilepsy patients from Penang General Hospital
    MeSH terms: Adolescent; Anticonvulsants/therapeutic use*; Benzodiazepines/therapeutic use*; Child; Child, Preschool; Cross-Sectional Studies; Drug Therapy, Combination; Drug Utilization; Female; Hospitals, General; Hospitals, Public/statistics & numerical data*; Humans; Infant; Malaysia; Male; Outpatient Clinics, Hospital; Seizures/drug therapy*; Treatment Outcome
  4. Hasnan J, Yusof MI, Damitri TD, Faridah AR, Adenan AS, Norbaini TH
    Singapore Med J, 2010 Jan;51(1):50-5.
    PMID: 20200776
    Bax is essential for apoptosis in normal cells. However, overexpression of Bcl-2 enhances cell survival by suppressing apoptosis in cells subjected to apoptosis-inducing stimuli. The aim of this study was to examine the expression of apoptotic (Bax and Bcl-2) and biochemical markers in type 2 diabetics mellitus.
    MeSH terms: Biopsy; Blood Glucose/metabolism; Cross-Sectional Studies; Diabetes Mellitus, Type 2/metabolism*; Hemoglobin A, Glycosylated/analysis; Humans; Cholesterol, HDL/blood; Cholesterol, LDL/blood; Skin/metabolism*; Biomarkers/metabolism; Case-Control Studies; Apoptosis*; Proto-Oncogene Proteins c-bcl-2/metabolism*; bcl-2-Associated X Protein/metabolism*
  5. Noradina AT, Karim NA, Hamidon BB, Norlinah I, Raymond AA
    Singapore Med J, 2010 Jan;51(1):60-4.
    PMID: 20200778
    Retraction notice: Following investigations of duplicate publication in Parkinsonism and Related Disorders 2009; 15: 670–674, we have determined that there is indeed a substantial overlap between the two articles. As such, we fully retract this paper from the published record of the Singapore Medical Journal.
    Professor Teo Eng Kiong, Editor, Singapore Medical Journal
    http://smj.sma.org.sg/5201/5201rec1.pdf

    There are limited studies reporting the frequency of sleep-disordered breathing (SDB) in Parkinson's disease (PD), and the figures quoted are variable, ranging from 2.5 to 66 percent. This study aimed to determine the prevalence and types of SBD in PD patients attending the Universiti Kebangsaan Malaysia Medical Centre neurology clinic, and the correlation between the subjective sleep symptoms using the Parkinson's disease sleep scale (PDSS) and the objective measurements using polysomnography (PSG).
    METHODS: This was a cross-sectional study involving 46 PD patients over a period of six months. The patients' demographic data, Hoehn and Yahr staging and PDSS scores were collected. The patients were then subjected to overnight PSG using the Somnomedic system.
    RESULTS: There were 27 male and 19 female patients with a mean age of 64.0+/-9.7 years. 29 were Chinese, 15 Malay and 2 Indian. The mean duration of illness was 5.8+/-4.3 years. The mean PDSS score was 120.3+/-13.5. SDB was found in 54.6 percent of the patients (apnoeahypopnoea index [AHI] 5 and above), with 27.3 percent having moderate and severe SDB (AHI 15 and above). The median AHI was 6.7 (range 0-40.4). The prevalence of SDB in PD patients based on the AHI cutoffs were 27.3 percent for mild, 18.2 percent for moderate and 9.1 percent for severe. There were statistically significant positive correlations between the AHI and the neck circumference and between the AHI and the waist-hip ratio. There was no significant correlation between the AHI and PDSS, or the AHI and disease severity.
    CONCLUSION: There was a high prevalence of SBD in our PD patients, which was comparable to other studies. Obstructive sleep apnoea was the dominant type of SBD. There was no correlation between the subjective sleep symptoms using the PDSS and the objective measurements using PSG.

    Study site: Neurology clinic, Pusat Perubatan Universiti Kebangsaan Malaysia (PPUKM)
    MeSH terms: Academic Medical Centers; Aged; Cross-Sectional Studies; Female; Hospitals, University; Humans; Malaysia/epidemiology; Male; Middle Aged; Parkinson Disease/complications*; Parkinson Disease/epidemiology; Prevalence; Polysomnography; Sleep Apnea, Obstructive/complications*; Sleep Apnea, Obstructive/epidemiology*; Sleep Apnea, Central/complications*; Sleep Apnea, Central/epidemiology*
  6. Rasoli M, Omar AR, Aini I, Jalilian B, Syed Hassan SH, Mohamed M
    Acta Virol., 2010;54(1):33-9.
    PMID: 20201612
    A series of plasmids containing the HSP70 gene of Mycobacterium tuberculosis fused to the hemagglutinin (H5) gene of H5N1 avian influenza virus (AIV) (H5-HSP70 (heat shock protein 70) vaccine) or individual H5 gene (H5 vaccine) or HSP70 gene (HSP70 vaccine) were constructed based on the plasmid pcDNA3.1. Expression of H5 gene in Vero cells in vitro and in chickens in vivo was confirmed following their transfection and immunization with H5 or H5-HSP70 vaccines. Controls consisted of HSP70 vaccine, empty plasmid pcDNA3.1 and co-administered H5 and HSP70 vaccines. H5-HSP70 vaccine produced in chicken higher hemagglutination inhibition (HI) antibody titer than H5 vaccine. However, the increase was not statistically significant. We have demonstrated for the first time that the H5 DNA vaccine with fused HSP70 gene may produce an enhanced induction of humoral immune response to AIV in chickens.
    MeSH terms: Animals; Antibodies, Viral/blood*; Cercopithecus aethiops; Chickens; Influenza in Birds/immunology; Influenza in Birds/prevention & control; Influenza in Birds/virology; Hemagglutination Inhibition Tests; Humans; Influenza Vaccines/administration & dosage; Influenza Vaccines/immunology; Plasmids; Poultry Diseases/immunology; Poultry Diseases/prevention & control; Poultry Diseases/virology; Vaccination/veterinary; Vero Cells; Influenza A Virus, H5N1 Subtype/immunology*
  7. How YF, Lee CY
    Med Vet Entomol, 2010 Jun;24(2):108-16.
    PMID: 20202109 DOI: 10.1111/j.1365-2915.2010.00852.x
    This study examined the fecundity, oviposition, nymphal development and longevity of field-collected samples of the tropical bedbug, Cimex hemipterus (Fabricius) (Hemiptera: Cimicidae). Under environmental conditions of 26+/-2 degrees C, 70 +/- 5% relative humidity and a 12-h photoperiod, with bloodmeals provided by a human host, six strains of tropical bedbug had a fecundity of up to 50 eggs per lifetime, over 11-14 oviposition cycles. Increased feeding frequency improved fecundity. After feeding and mating, adult females normally took 2-3 days to produce a first batch of eggs. The oviposition period lasted 2-7 days before cessation of the oviposition cycle. The egg incubation period usually lasted 5-7 days before the emergence of first instars. The nymphs underwent five stadia (the first four of which each took 3-4 days, whereas the last took 4-5 days) before becoming adults at a sex ratio of 1 : 1. More than five bloodmeals were required by the nymphs to ensure a successful moult. Unmated adults lived significantly longer than mated adults (P < 0.05). Unmated females lived up to almost 7 months, but the longevity of mated males and females did not differ significantly (P > 0.05).
    MeSH terms: Animals; Bedbugs/growth & development; Bedbugs/physiology*; Female; Fertility/physiology; Humans; Longevity/physiology*; Male; Nymph/growth & development; Oviposition/physiology; Sex Ratio; Tropical Climate*; Body Size/physiology
  8. Zaini NA, Harith HH, Olusesan AT, Zulkifli AH, Bakar FA, Osman A, et al.
    J Food Prot, 2010 Mar;73(3):541-6.
    PMID: 20202342
    The objective of this study was to determine the level of preservatives and microbiological loads in various brands of commercially available chili bo (paste). Fifteen different brands of chili bo obtained from the local market and hypermarkets were analyzed for pH, moisture and benzoic acid content, microbiological loads (aerobic, anaerobic, aerobic spores, and fungi), and thermophilic microorganisms. Results showed that both moisture content and pH vary among samples. The concentrations of benzoic acid detected in chili bo were found to be in the range of 537 to 5,435 mg/kg. Nine of fifteen brands were found to exceed the maximum level permitted by the Malaysian Food Law in accordance with the Codex Alimentarius (1,000 mg/kg for benzoic acid). An apparent correlation between benzoic acid concentration and microbiological loads present in the chili bo was observed. The microbiological loads were found to be relatively low in the end products containing high amounts of benzoic acid. The heat-resistant (70 to 80 degrees C) microorganisms present in chili bo were identified as Ochrobacterum tritici, Stenotrophomonas rhizophila, Microbacterium maritypicum, Roseomonas spp., CDC group II-E subgroup A, Flavimonas oryzihabitans, and Pseudomonas aeruginosa, with M. maritypicum being the most frequently found (in 9 of 15 samples) microorganism. Most of these identified microorganisms were not known to cause foodborne illnesses.
    MeSH terms: Bacteria/growth & development; Bacteria/isolation & purification*; Capsicum/microbiology*; Capsicum/chemistry*; Consumer Product Safety*; Food Contamination/analysis*; Food Microbiology; Fungi/growth & development; Fungi/isolation & purification; Humans; Hydrogen-Ion Concentration; Malaysia; Water/metabolism; Benzoic Acid/analysis
  9. Sutan R, Amin RM, Ariffin KB, Teng TZ, Kamal MF, Rusli RZ
    J Zhejiang Univ Sci B, 2010 Mar;11(3):209-17.
    PMID: 20205307 DOI: 10.1631/jzus.B0900245
    To evaluate the psychosocial impact among mothers with perinatal loss and its contributing factors.
    MeSH terms: Adult; Depression/epidemiology*; Depression/psychology*; Educational Status; Female; Humans; Malaysia/epidemiology; Mothers/psychology*; Mothers/statistics & numerical data*; Psychology; Surveys and Questionnaires; Social Support; Age Distribution; Stillbirth/epidemiology*; Stillbirth/psychology*; Perinatal Mortality*; Young Adult
  10. Tran NT, Jang MC, Choe YS, Ko WS, Pyo HS, Kim OS
    Int J Gynaecol Obstet, 2010 Jun;109(3):209-12.
    PMID: 20206354 DOI: 10.1016/j.ijgo.2010.01.012
    To examine the feasibility, efficacy, safety, and acceptability of medical abortion among rural and urban women up to 56 days of pregnancy in the Democratic People's Republic of Korea.
    MeSH terms: Abortifacient Agents, Nonsteroidal/administration & dosage; Abortifacient Agents, Nonsteroidal/adverse effects; Abortifacient Agents, Nonsteroidal/therapeutic use; Abortifacient Agents, Steroidal/administration & dosage; Abortifacient Agents, Steroidal/adverse effects; Abortifacient Agents, Steroidal/therapeutic use; Abortion, Induced/methods*; Administration, Oral; Administration, Sublingual; Adult; Drug Therapy, Combination; Feasibility Studies; Female; Humans; Pilot Projects; Pregnancy; Mifepristone/administration & dosage; Mifepristone/adverse effects; Mifepristone/therapeutic use*; Misoprostol/administration & dosage; Misoprostol/adverse effects; Misoprostol/therapeutic use*; Patient Satisfaction; Rural Health Services; Urban Health Services; Democratic People's Republic of Korea
  11. Naing CM, Win DK
    Trans R Soc Trop Med Hyg, 2010 May;104(5):311-2.
    PMID: 20206954 DOI: 10.1016/j.trstmh.2010.02.001
    Permanent neurological impairment or death arising from hospital-acquired hyponatremia in both children and adults is well documented. The choice of intravenous fluids for fluid resuscitation in critically ill patients is a top priority in evidence-based medicine. The question of whether colloids in comparison to crystalloids can improve mortality in such cases remains to be answered. Well powered, randomized clinical trials addressing the comparative efficacy of different types of intravenous fluids is a high priority as is the ethical justification for such trials. The understanding of the pathophysiological process serves important information on clinical practice.
    MeSH terms: Adult; Child; Colloids/adverse effects; Colloids/therapeutic use*; Fluid Therapy/adverse effects; Fluid Therapy/methods*; Humans; Hyponatremia/etiology; Hyponatremia/mortality; Infusions, Intravenous; Isotonic Solutions/adverse effects; Isotonic Solutions/therapeutic use*; Plasma Substitutes/adverse effects; Plasma Substitutes/therapeutic use*; Resuscitation/methods; Critical Illness/mortality; Critical Illness/therapy*
  12. Mumtaz T, Khan MR, Hassan MA
    Micron, 2010 Jul;41(5):430-8.
    PMID: 20207547 DOI: 10.1016/j.micron.2010.02.008
    An outdoor soil burial test was carried out to evaluate the degradation of commercially available LDPE carrier bags in natural soil for up to 2 years. Biodegradability of low density polyethylene films in soil was monitored using both optical and scanning electron microscopy (SEM). After 7-9 months of soil exposure, microbial colonization was evident on the film surface. Exposed LDPE samples exhibit progressive changes towards degradation after 17-22 months. SEM images reveal signs of degradation such as exfoliation and formation of cracks on film leading to disintegration. The possible degradation mode and consequences on the use and disposal of LDPE films is discussed.
    MeSH terms: Biodegradation, Environmental; Microscopy; Microscopy, Electron, Scanning; Soil Microbiology*; Time Factors; Polyethylene/metabolism*
  13. Tuan Zainazor C, Hidayah MS, Chai LC, Tunung R, Ghazali FM, Son R
    J Microbiol Biotechnol, 2010 Feb;20(2):229-37.
    PMID: 20208424
    Recently, many cases related to viral gastroenteritis outbreaks have been reported all over the world. Noroviruses are found to be leading as the major cause of outbreaks of acute gastroenteritis. Patients with the acute gastroenteritis normally found to be positive with norovirus when stools and vomit were analyzed. This paper reviews various activities and previous reports that describe norovirus contaminated in various food matrixes and relationship between food handlers. Lately, a numbers of norovirus outbreaks have been reported which are involved fresh produce (such as vegetables, fruits), shellfish and prepared food. Food produces by infected food handlers may therefore easily contaminated. In addition, food that required much handling and have been eaten without heat treatment gave the high risk for getting foodborne illnesses. The standard method for detection of norovirus has already been available for stool samples. However, only few methods for detection of norovirus in food samples have been developed until now.
    MeSH terms: Disease Outbreaks; Food Analysis; Food Contamination/analysis*; Food Handling*; Humans; Caliciviridae Infections/microbiology*; Caliciviridae Infections/epidemiology; Norovirus/genetics; Norovirus/isolation & purification*
  14. Tunung R, Margaret S, Jeyaletchumi P, Chai LC, Tuan Zainazor TC, Ghazali FM, et al.
    J Microbiol Biotechnol, 2010 Feb;20(2):391-6.
    PMID: 20208446
    The purpose of this study was to investigate the biosafety of Vibrio parahaemolyticus in raw salad vegetables at wet market and supermarket in Malaysia. A combination of Most Probable Number - Polymerase Chain Reaction (MPN-PCR) method was applied to detect the presence of V. parahaemolyticus and to enumerate their density in the food samples. The study analyzed 276 samples of common vegetables eaten raw in Malaysia (Wild cosmos = 8; Japanese parsley = 21; Cabbage = 30; Lettuce = 16; Indian pennywort = 17; Carrot = 31; Sweet potato = 29; Tomato = 38; Cucumber = 28; Four winged bean = 26; Long bean = 32). The samples were purchased from two supermarkets (A and B) and two wet markets (C and D). The occurrence of V. parahaemolyticus detected was 20.65%, with higher frequency of V. parahaemolyticus in vegetables obtained from wet markets (Wet market C = 27.27%Wet Market D = 32.05%) compared to supermarkets (Supermarket A = 1.64%; Supermarket B = 16.67%). V. parahaemolyticus was most prevalent in Indian pennywort (41.18%). The density of V. parahaemolyticus in all the samples ranged from <3 up to >2400 MPN/g, mostly <3 MPN/g concentration. Raw vegetables from wet markets contained higher levels of V. parahaemolyticus compared to supermarkets. V. parahaemolyticus were present in raw vegetables although in low numbers. The results suggest that raw vegetables act as a transmission route for V. parahaemolyticus. This study will be the first biosafety assessment of V. parahaemolyticus in raw vegetables in Malaysia.
    MeSH terms: Consumer Product Safety*; Food Contamination/analysis*; Vegetables/microbiology*; Vibrio parahaemolyticus/genetics; Vibrio parahaemolyticus/isolation & purification*
  15. Wkly. Epidemiol. Rec., 2010 Feb 19;85(8):64-7.
    PMID: 20210044
    MeSH terms: Animals; Animals, Domestic/virology; Bangladesh/epidemiology; Disease Vectors; Humans; India/epidemiology; Malaysia/epidemiology; Singapore/epidemiology; Zoonoses/epidemiology*; Zoonoses/transmission; Zoonoses/virology; Nipah Virus*; Henipavirus Infections/epidemiology*; Henipavirus Infections/prevention & control; Henipavirus Infections/veterinary
  16. Md Ghani NA, Liong CY, Jemain AA
    Forensic Sci Int, 2010 May 20;198(1-3):143-9.
    PMID: 20211535 DOI: 10.1016/j.forsciint.2010.02.011
    The task of identifying firearms from forensic ballistics specimens is exacting in crime investigation since the last two decades. Every firearm, regardless of its size, make and model, has its own unique 'fingerprint'. These fingerprints transfer when a firearm is fired to the fired bullet and cartridge case. The components that are involved in producing these unique characteristics are the firing chamber, breech face, firing pin, ejector, extractor and the rifling of the barrel. These unique characteristics are the critical features in identifying firearms. It allows investigators to decide on which particular firearm that has fired the bullet. Traditionally the comparison of ballistic evidence has been a tedious and time-consuming process requiring highly skilled examiners. Therefore, the main objective of this study is the extraction and identification of suitable features from firing pin impression of cartridge case images for firearm recognition. Some previous studies have shown that firing pin impression of cartridge case is one of the most important characteristics used for identifying an individual firearm. In this study, data are gathered using 747 cartridge case images captured from five different pistols of type 9mm Parabellum Vektor SP1, made in South Africa. All the images of the cartridge cases are then segmented into three regions, forming three different set of images, i.e. firing pin impression image, centre of firing pin impression image and ring of firing pin impression image. Then geometric moments up to the sixth order were generated from each part of the images to form a set of numerical features. These 48 features were found to be significantly different using the MANOVA test. This high dimension of features is then reduced into only 11 significant features using correlation analysis. Classification results using cross-validation under discriminant analysis show that 96.7% of the images were classified correctly. These results demonstrate the value of geometric moments technique for producing a set of numerical features, based on which the identification of firearms are made.
    MeSH terms: Crime; Face; Firearms; Research Personnel; South Africa; Multivariate Analysis; Discriminant Analysis; Forensic Ballistics; Educational Personnel
  17. Katherason SG, Naing L, Jaalam K, Nik Mohamad NA, Bhojwani K, Harussani ND, et al.
    J Infect Dev Ctries, 2010 Mar 08;4(2):118-23.
    PMID: 20212345
    BACKGROUND: Hand decontamination is a critical infection control practice in the prevention of nosocomial infection. This study was conducted to observe the hand hygiene practices of nurses and doctors in two intensive care units (ICUs) in Malaysia.

    METHODOLOGY: Staff members were observed during patient contacts, and their hand washing techniques and hand hygiene practices were monitored. Five contact periods were observed for staff members while they cared for their assigned patients. Hand hygiene practices before and after patient contacts were categorized as clean uncontaminated, clean recontaminated, new gloves, and unchanged contaminated gloves. Compliance to hand-washing steps and time taken for hand washing were analyzed. Appropriate use of gloves based on CDC criteria also was assessed.

    RESULTS: Compliance to hand hygiene practices was 70% before each patient contact. Staff members did not completely adhere to the hand-washing steps. The average time taken to wash hands was 20 seconds, and the necessary steps (rubbing palm over dorsum; rubbing fingers interlaced, and rotational rubbing of thumbs) were practiced minimally by all staff. Hand washing protocol was generally followed by all staff (100%). Alcohol hand rubs were available but were used moderately (60%); when used, staff members did not wait for the alcohol to dry. Only 4% of staff changed contaminated gloves between patients.

    CONCLUSIONS: Hand hygiene compliance by ICU staff members needs to be improved. Improving adherence to correct hand hygiene techniques will require effective education programs and behavioral modification techniques. Moreover, hand hygiene guidelines must be incorporated into new staff orientation programs and the continuing education curriculum in the two hospitals studied.

    MeSH terms: Adult; Decontamination/statistics & numerical data*; Female; Humans; Intensive Care Units/standards; Intensive Care Units/statistics & numerical data*; Malaysia; Male; Time Factors; Infection Control/methods; Infection Control/standards; Infection Control/statistics & numerical data*; Gloves, Protective/statistics & numerical data*; Guideline Adherence/statistics & numerical data
  18. Lau KA, Wang B, Miranda-Saksena M, Boadle R, Kamarulzaman A, Ng KP, et al.
    Curr HIV Res, 2010 Apr;8(3):259-71.
    PMID: 20214658
    In Malaysia, co-circulation of CRF01_AE and subtype B has resulted in the emergence of the second generation derivative; CRF33_01B in approximately 20% of its HIV-1 infected individuals. Our objective was to identify possible biological advantages that CRF33_01B possesses over its progenitors. Biological and molecular comparisons of CRF33_01B against its parental subtypes clearly show that CRF33_01B replicated better in activated whole peripheral blood mononuclear cells (PBMCs) and CD4+ T-lymphocytes, but not monocyte-derived macrophages (MDMs). Also, its acquired fitness was greater than CRF01_AE but not subtype B. Moreover, CRF33_01B has higher rate of apoptotic cell death and syncytia induction compared to subtype B. These adaptive and survival abilities could have been acquired by CRF33_01B due to the incorporation of subtype B fragments into the gag-RT region of its full-length genome. Our studies confirm the previously held belief that HIV-1 strains may harbor enhanced biological fitness upon recombination. We therefore estimate a possible gradual replacement of the current predominance of CRF01_AE, as well as wider dissemination of CRF33_01B, together with the identification of other new CRF01_AE/B inter-subtype recombinants in Malaysia.
    MeSH terms: Cells, Cultured; Genotype; Humans; Leukocytes, Mononuclear/virology; Macrophages/virology; Malaysia/epidemiology; Recombination, Genetic; Virus Replication; CD4-Positive T-Lymphocytes/virology; HIV-1/classification*; HIV-1/genetics; HIV-1/isolation & purification; HIV-1/pathogenicity*; HIV Infections/epidemiology*; HIV Infections/virology*; Giant Cells; Apoptosis
  19. Kiew LV, Cheong SK, Sidik K, Chung LY
    Int J Pharm, 2010 May 31;391(1-2):212-20.
    PMID: 20214970 DOI: 10.1016/j.ijpharm.2010.03.010
    To enhance the stability of the anticancer drug gemcitabine (2'-deoxy-2',2'-difluorocytidine), it was conjugated to poly-l-glutamic acid (PG-H) via a carbodiimide reaction. The synthesised poly-l-glutamic acid-gemcitabine (PG-G) was purified and characterised by using SDS-PAGE to estimate its molecular weight, HPLC to determine its purity and degree of drug loading, and NMR to elucidate the structure. In vitro aqueous hydrolytic studies showed that the gemcitabine release from the polymeric drug conjugate was pH dependent, and that the conjugation to PG-H improved its stability in human plasma. The release of the bound gemcitabine from PG-G in plasma was mediated by a hydrolytic process. It began with a lag phase, followed by linear release between 12 and 48h, and reached equilibrium at 72h with 51% of the gemcitabine released. In vitro cytotoxicity studies using MCF-7 and MDA-MB-231 human mammary cancer cells, as well as human dermal fibroblasts (HDF), showed that PG-G displayed a lower dose dependent cytotoxic effect with respect to the parent drug gemcitabine. On the other hand, in 4T1 mouse mammary tumour cells, PG-G and gemcitabine showed similar toxicities. Gemcitabine was more than likely released hydrolytically from PG-G and taken up by MCF-7, MDA-MB-231 and HDF, whereas both released gemcitabine and PG-G were taken up by 4T1 to mediate the observed cytotoxicities. The improved stability and extended sustained release profile may render PG-G a potential anticancer prodrug.
    MeSH terms: Animals; Cell Line; Cell Survival/drug effects; Delayed-Action Preparations; Deoxycytidine/administration & dosage; Deoxycytidine/analogs & derivatives*; Deoxycytidine/blood; Deoxycytidine/chemical synthesis; Deoxycytidine/pharmacokinetics; Deoxycytidine/pharmacology; Deoxycytidine/chemistry; Drug Screening Assays, Antitumor; Drug Stability; Female; Humans; Polyglutamic Acid/administration & dosage; Polyglutamic Acid/analogs & derivatives*; Polyglutamic Acid/blood; Polyglutamic Acid/chemical synthesis; Polyglutamic Acid/pharmacokinetics; Polyglutamic Acid/pharmacology; Prodrugs/administration & dosage; Prodrugs/chemical synthesis*; Prodrugs/pharmacokinetics*; Prodrugs/pharmacology; Cell Line, Tumor
  20. Baig MR, Rajan G
    J Oral Implantol, 2010;36(1):31-5.
    PMID: 20218868 DOI: 10.1563/AAID-JOI-D-09-00062
    This article describes the dental implant-based rehabilitation of a partially edentulous patient with a unilateral maxillary dento-alveolar defect. A screw-retained prosthesis with a modified design was fabricated on zygomatic and regular dental implants. One section of the implant prosthesis has cemented crowns and the other section is conventional screw-retained. The design of the prosthesis overcame the hard and soft tissue deficit and provided the desired esthetics.
    MeSH terms: Adult; Cementation; Crowns; Dental Implantation, Endosseous/methods*; Denture Retention/instrumentation*; Denture Retention/methods*; Denture, Partial, Fixed*; Female; Humans; Jaw, Edentulous, Partially/rehabilitation*; Jaw, Edentulous, Partially/surgery; Maxilla/surgery; Zygoma/surgery*; Alveolar Bone Loss/rehabilitation; Dental Prosthesis Design; Dental Prosthesis, Implant-Supported*
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