Browse publications by year: 2011

  1. Wee LH, Shahab L, Bulgiba A, West R
    Addict Behav, 2011 Apr;36(4):400-3.
    PMID: 21195553 DOI: 10.1016/j.addbeh.2010.11.011
    INTRODUCTION: As Stop Smoking Clinics (SSCs) become more common across the globe, it is important to know how far one can make broad generalisations concerning characteristics of smokers who attend these clinics and factors that predict their success. This involves accumulation of data from different countries.
    OBJECTIVE: The aim of this study was to identify characteristics of smokers and factors leading up to quitting with clinics in Malaysia.
    METHOD: Records from 629 smokers who had sought help from five selected SSCs in Malaysia from January 2006 to June 2007 were analysed.
    RESULTS: The characteristics of smokers attending Malaysian smoking clinics were broadly similar to those in Western countries. Consistent with the findings from other countries, older age and longest duration of previous quit attempts were associated with successful smoking cessation. Greater baseline carbon-monoxide readings (OR 0.96, 95% CI 0.93-0.99; p=0.013), but not Fagerstrom Test for Nicotine Dependence (FTND), predicted failure to quit at six-month in multivariate analysis. Success rates varied greatly between clinics even after adjusting for all other predictors.
    CONCLUSION: In these rare data from a non-Western culture some predictors of successful smoking cessation appeared to generalise from Western smokers but the universal validity of the FTND in particular needs to be examined further.
    MeSH terms: Adolescent; Adult; Ambulatory Care Facilities*; Carbon Monoxide/analysis; Demography; Female; Health Status; Humans; Malaysia; Male; Middle Aged; Patient Acceptance of Health Care; Surveys and Questionnaires; Retrospective Studies; Smoking/therapy*; Tobacco Use Disorder/diagnosis; Cohort Studies; Smoking Cessation/statistics & numerical data*; Treatment Outcome; Young Adult
  2. Shahrizaila N, Yuki N
    J Biomed Biotechnol, 2011;2011:829129.
    PMID: 21197269 DOI: 10.1155/2011/829129
    Molecular mimicry between self and microbial components has been proposed as the pathogenic mechanism of autoimmune diseases, and this hypothesis is proven in Guillain-Barré syndrome. Guillain-Barré syndrome, the most frequent cause of acute neuromuscular paralysis, sometimes occurs after Campylobacter jejuni enteritis. Gangliosides are predominantly cell-surface glycolipids highly expressed in nervous tissue, whilst lipo-oligosaccharides are major components of the Gram-negative bacterium C. jejuni outer membrane. IgG autoantibodies to GM1 ganglioside were found in the sera from patients with Guillain-Barré syndrome. Molecular mimicry was demonstrated between GM1 and lipo-oligosaccharide of C. jejuni isolated from the patients. Disease models by sensitization of rabbits with GM1 and C. jejuni lipo-oligosaccharide were established. Guillain-Barré syndrome provided the first verification that an autoimmune disease is triggered by molecular mimicry. Its disease models are helpful to further understand the molecular pathogenesis as well as to develop new treatments in Guillain-Barré syndrome.
    MeSH terms: Animals; Disease Models, Animal*; Humans; Rabbits; Molecular Mimicry*; Guillain-Barre Syndrome/pathology*
  3. Teng KT, Nagapan G, Cheng HM, Nesaretnam K
    Lipids, 2011 Apr;46(4):381-8.
    PMID: 21197586 DOI: 10.1007/s11745-010-3516-y
    Postprandial lipemia impairs insulin sensitivity and triggers the pro-inflammatory state which may lead to the progression of cardiovascular diseases. A randomized, crossover single-blind study (n = 10 healthy men) was designed to compare the effects of a high-fat load (50 g fat), rich in palmitic acid from both plant (palm olein) or animal source (lard) versus an oleic acid-rich fat (virgin olive oil) on lipemia, plasma glucose, insulin and adipocytokines. Serum triacylglycerol (TAG) concentrations were significantly lower after the lard meal than after the olive oil and palm olein meals (meal effect P = 0.003; time effect P < 0.001). The greater reduction in the plasma non-esterified free fatty acids levels in the lard group compared to the olive oil meal was mirrored by the changes observed for serum TAG levels (P < 0.05). The magnitude of response for plasma glucose, insulin and adipocytokines [interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and leptin] were not altered by the type of dietary fats. A significant difference in plasma IL-1β was found over time following the three high fat loads (time effect P = 0.036). The physical characteristics and changes in TAG structure of lard may contribute to the smaller increase in postprandial lipemia compared with palm olein. A high fat load but not the type of fats influences concentrations of plasma IL-1β over time but had no effect on other pro-inflammatory markers tested in the postprandial state.
    MeSH terms: Olive Oil; Animals; Blood Glucose/metabolism*; Dietary Fats/administration & dosage; Dietary Fats/metabolism*; Humans; Hyperlipidemias/blood; Insulin/blood*; Male; Plant Oils/administration & dosage*; Single-Blind Method; Cross-Over Studies; Postprandial Period*; Adipokines/blood*; Young Adult
  4. Ogawa O
    Int J Urol, 2011 Jan;18(1):87-91.
    PMID: 21198942 DOI: 10.1111/j.1442-2042.2010.02659.x
    As part of the 20th Anniversary Celebration of the Urological Association of Asia (UAA), the Asian School of Urology, Young Leaders' Workshop was held in Kyoto, 23-25 April 2010. The workshop focused on future national Asian leaders in the field of Urology and was arranged by the Asian School of Urology (ASU), an educational branch of the UAA, and Dr Osamu Ogawa, the workshop Organizer. Urologists from several Japanese universities contributed to the workshop as advisors, taskforce members, and group members. The workshop was also actively supported by Dr Allen Chiu (Taiwan) and Dr Stephen Lim (Singapore). A younger generation of urologists was invited to attend the workshop and, in all, 29 delegates from 17 countries (Australia, Bangladesh, Cambodia, China, India, Indonesia, Iran, Korea, Malaysia, Myanmar, Nepal, Philippines, Singapore, Sri Lanka, Taiwan, Thailand, and Vietnam), chosen on the basis of recommendations from local councils, attended. The purpose of the workshop was to open channels of communication between future Asian leaders in Urology. The workshop topic was "Asian Clinical Guidelines". Workshop participants were divided into five groups addressing distinct issues associated with the notion of clinical guidelines: Group A, General Problems; Group B, Prostate Cancer; Group C, Stone Disease; Group D, Infectious Diseases; and Group E, Bladder Cancer. The workshop consisted of an introductory session, followed by three main sessions, each of which consisted of a group discussion of specific problems associated with the establishment of Asian Guidelines for Urological Diseases and how best to deal with them, followed by a plenary presentation of the outcomes.
    MeSH terms: Asia; Societies, Medical; Urology/standards*; Practice Guidelines as Topic*
  5. Kohyama J, Mindell JA, Sadeh A
    Pediatr Int, 2011 Oct;53(5):649-655.
    PMID: 21199167 DOI: 10.1111/j.1442-200X.2010.03318.x
    BACKGROUND: A recent international Internet-based study of young children (birth to 36 months) found that total sleep duration in Japan was the shortest among 17 countries/regions. The present study compared features of children's sleep in Japan relative to those in other Asian countries/regions.

    METHODS: Parents of 872 infants and toddlers in Japan (48.6% boys), and parents of 20 455 infants and toddlers in 11 other Asian countries/regions (48.1% boys; China, Hong Kong, India, Indonesia, Korea, Malaysia, Philippines, Singapore, Taiwan, Thailand, and Vietnam) completed an Internet-based expanded version of the Brief Infant Sleep Questionnaire.

    RESULTS: Young children in Japan exhibited significantly fewer nocturnal wakings and shorter daytime sleep in comparison with other Asian countries/regions. Although the former finding was apparent in all age groups, the reduced duration of daytime sleep in Japan was not present until after 3 months of age. Interestingly, sleep problems were reported by significantly fewer parents in Japan compared with those in other Asian countries/regions, although parents in Japan reported significantly more difficulty at bedtime.

    CONCLUSIONS: The short sleep duration of young children in Japan is largely due to a relatively short duration of daytime sleep. Significant differences in sleep characteristics in Japan relative to other Asian regions were found primarily after 3 months of age. Future studies should further explore the underlying causes and the potential impacts of these sleep differences.

    MeSH terms: Adult; Asia, Southeastern; Child, Preschool; Cross-Cultural Comparison*; Female; Humans; Infant; Infant, Newborn; Japan; Male; Surveys and Questionnaires; Sleep/physiology*; Wakefulness
  6. Langarizadeh M, Mahmud R, Ramli AR, Napis S, Beikzadeh MR, Rahman WE
    J Med Eng Technol, 2011 Feb;35(2):103-8.
    PMID: 21204610 DOI: 10.3109/03091902.2010.542271
    Breast cancer is one of the most important diseases in females worldwide. According to the Malaysian Oncological Society, about 4% of women who are 40 years old and above are involved have breast cancer. Masses and microcalcifications are two important signs of breast cancer diagnosis on mammography. Enhancement techniques, i.e. histogram equalization, histogram stretching and median filters, were used to provide better visualization for radiologists in order to help early detection of breast abnormalities. In this research 60 digital mammogram images which includes 20 normal and 40 confirmed diagnosed cancerous cases were selected and manipulated using the mentioned techniques. The original and manipulated images were scored by three expert radiologists. Results showed that the selected methods have a positive significant effect on image quality.
    MeSH terms: Female; Humans; Mammography/methods*; Radiographic Image Enhancement
  7. Khodavandi A, Alizadeh F, Harmal NS, Sidik SM, Othman F, Sekawi Z, et al.
    FEMS Microbiol Lett, 2011 Feb;315(2):87-93.
    PMID: 21204918 DOI: 10.1111/j.1574-6968.2010.02170.x
    The efficacy of allicin compared with fluconazole in alleviating systemic Candida albicans infections was evaluated both in vitro and in vivo through a systemic candidiasis mouse model. Determination of in vitro minimum inhibitory concentrations (MICs) for different C. albicans isolates revealed that both allicin and fluconazole showed different MICs that ranged from 0.05 to 12.5 μg mL(-1) and 0.25 to 16 μg mL(-1) , respectively. A time-kill study showed a significant effect of allicin (P<0.01) against C. albicans, comparable to that of fluconazole. Scanning electron microscopy observation revealed that, similar to fluconazole, allicin produced structural destruction of C. albicans cell surface at low MIC and lysis or puncture at high MIC concentrations. Treatment of BALB/c mice systemically infected with C. albicans showed that although the allicin treatment (at 5 mg kg(-1) day(-1) ) was slightly less efficacious than fluconazole treatment in terms of the fungal load reduction and host survival time, it was still effective against C. albicans in terms of mean survival time, which increased from 8.4 to 15.8 days. These results demonstrate the efficacy of anticandidal effects of allicin both in vitro and in an animal model of candidiasis and affirm the potential of allicin as an adjuvant therapy to fluconazole.
    MeSH terms: Animals; Candida albicans/cytology; Candida albicans/drug effects*; Candidiasis/drug therapy; Candidiasis/mortality; Disease Models, Animal; Female; Humans; Mice, Inbred BALB C; Microbial Sensitivity Tests; Microscopy, Electron, Scanning; Sulfinic Acids/pharmacology*; Sulfinic Acids/therapeutic use*; Fluconazole/pharmacology*; Fluconazole/therapeutic use*; Survival Analysis; Treatment Outcome; Microbial Viability/drug effects; Mice
  8. Suleiman Gwaram N, Khaledi H, Ali HM, Olmstead MM
    Acta Crystallogr C, 2011 Jan;67(Pt 1):o6-9.
    PMID: 21206084 DOI: 10.1107/S0108270110046317
    The title compound, C(4)H(6)N(4)O·H(2)O, crystallized simultaneously as a triclinic and a monoclinic polymorph from an aqueous solution of 2,4-diaminopyrimidin-6-ol. Previously, an orthorhombic polymorph was isolated under the same experimental conditions. The molecular geometric parameters in the two present polymorphs and the previously reported orthorhombic polymorph are similar, but the structures differ in the details of their crystal packing. In the triclinic system, the diaminopyrimidinone molecules are connected to one another via N-H···O and N-H···N hydrogen bonding to form infinite chains in the [011] direction. The chains are further hydrogen bonded to the water molecules, resulting in a three-dimensional network. In the monoclinic system, the diaminopyrimidinone molecules are hydrogen bonded together into two-dimensional networks parallel to the bc plane. The water molecules link the planes to form a three-dimensional polymeric structure.
    MeSH terms: Crystallization; Hydrogen Bonding; Ions/chemistry*; Polymers/chemistry; Pyridines/chemistry*; Water/chemistry*; Molecular Structure; Crystallography, X-Ray
  9. Zahari Z, Teh LK, Ismail R, Razali SM
    Psychiatr Genet, 2011 Aug;21(4):183-9.
    PMID: 21206399 DOI: 10.1097/YPG.0b013e3283437250
    Variations in the gene for dopamine D2 receptor (DRD2) might have an influence on the outcome of antipsychotic treatment in schizophrenia. The objective of this study was to investigate the influence of DRD2 polymorphisms on treatment outcomes in patients with schizophrenia.
    MeSH terms: Adolescent; Adult; Alleles; Demography; Female; Humans; Male; Middle Aged; Polymorphism, Genetic*; Schizophrenia/drug therapy*; Schizophrenia/genetics*; Antipsychotic Agents/therapeutic use; Treatment Outcome; Receptors, Dopamine D2/genetics*; Genetic Predisposition to Disease; Young Adult
  10. Ismail AH, Jaafar MS
    Appl Radiat Isot, 2011 Mar;69(3):559-66.
    PMID: 21208807 DOI: 10.1016/j.apradiso.2010.11.004
    Complete blood counts were analyzed for 30 samples of human blood with radiation dose rate ranging between 10 and 41 μSv/h using a Radium-226 source with different time of exposure. A new technique involving a nuclear track detector type CR-39(CR-39 NTDs) was used to estimate the alpha particle density incident on the blood samples. The results show that the ranges of alpha particle in blood samples and on the surface of CR-39NTDs vary exponentially with energy of alpha particles. This depends on the restricted energy loss and target density. Changes in the blood components due to irradiation occurred for different durations of irradiation, and the duration of irradiation that influenced the blood samples in this study was 6 min. The change in red blood cell (RBC) was negligible, so it is less affected than other blood components. In addition, most changes in the blood contents began at a low radiation dose (10.38-13.41 μSv/h). For the doses 13.41-21.77 μSv/h, platelet (PLT) counts increased rapidly and adversely with the RBC and white blood cell (WBC) due to chromosomal aberration. Besides, rapid PLT count reduction rapidly at high dose (42.1 μSvh) causes thrombocytopenia; in contrast, WBC increased, which is an indication of cancer caused due to increase in alpha particle dose. Generally, our results are in agreement with the essentials of blood content and the principles of biological radiation interaction.
    MeSH terms: Adult; Alpha Particles; Blood/radiation effects*; Blood Cell Count; Female; Humans; Leukocytes/radiation effects; Male; Platelet Count; Radiometry; Radium
  11. Lee SK, Sirajudeen KN, Sundaram A, Zakaria R, Singh HJ
    J Physiol Biochem, 2011 Jun;67(2):249-57.
    PMID: 21210316 DOI: 10.1007/s13105-010-0070-2
    Although melatonin lowers blood pressure in spontaneously hypertensive rats (SHR), its effect following antenatal and postpartum supplementation on the subsequent development of hypertension in SHR pups remains unknown. To investigate this, SHR dams were given melatonin in drinking water (10 mg/kg body weight/day) from day 1 of pregnancy until day 21 postpartum. After weaning, a group of male pups continued to receive melatonin till the age of 16 weeks (Mel-SHR), while no further melatonin was given to another group of male pups (Maternal-Mel-SHR). Controls received plain drinking water. Systolic blood pressure (SBP) was measured at 4, 6, 8, 12 and 16 weeks of age, after which the kidneys were collected for analysis of antioxidant enzyme profiles. SBP was significantly lower till the age of 8 weeks in Maternal-Mel-SHR and Mel-SHR than that in the controls, after which no significant difference was evident in SBP between the controls and Maternal-Mel-SHR. SBP in Mel-SHR was lower than that in controls and Maternal-Mel-SHR at 12 and 16 weeks of age. Renal glutathione peroxidase (GPx) and glutathione s-transferase (GST) activities, levels of total glutathione and relative GPx-1 protein were significantly higher in Mel-SHR. GPx protein was however significantly higher in Mel-SHR. No significant differences were evident between the three groups in the activities of superoxide dismutase, catalase and glutathione reductase. In conclusion, it appears that while antenatal and postpartum melatonin supplementation decreases the rate of rise in blood pressure in SHR offspring, it however does not alter the tendency of offspring of SHR to develop hypertension.
    MeSH terms: Animals; Antioxidants/administration & dosage*; Antioxidants/pharmacology; Blood Pressure/drug effects*; Female; Glutathione Peroxidase/metabolism; Glutathione Transferase/metabolism; Hypertension/pathology*; Kidney/enzymology*; Kidney/metabolism; Male; Melatonin/administration & dosage*; Melatonin/pharmacology; Pregnancy; Rats, Inbred SHR; Weaning; Thiobarbituric Acid Reactive Substances/metabolism; Postpartum Period/cerebrospinal fluid; Postpartum Period/metabolism*; Rats
  12. Reidpath DD, Allotey P, Pokhrel S
    Health Res Policy Syst, 2011 Jan 06;9:1.
    PMID: 21210997 DOI: 10.1186/1478-4505-9-1
    BACKGROUND: There are strong arguments for social science and interdisciplinary research in the neglected tropical diseases. These diseases represent a rich and dynamic interplay between vector, host, and pathogen which occurs within social, physical and biological contexts. The overwhelming sense, however, is that neglected tropical diseases research is a biomedical endeavour largely excluding the social sciences. The purpose of this review is to provide a baseline for discussing the quantum and nature of the science that is being conducted, and the extent to which the social sciences are a part of that.

    METHODS: A bibliographic analysis was conducted of neglected tropical diseases related research papers published over the past 10 years in biomedical and social sciences. The analysis had textual and bibliometric facets, and focussed on chikungunya, dengue, visceral leishmaniasis, and onchocerciasis.

    RESULTS: There is substantial variation in the number of publications associated with each disease. The proportion of the research that is social science based appears remarkably consistent (<4%). A textual analysis, however, reveals a degree of misclassification by the abstracting service where a surprising proportion of the "social sciences" research was pure clinical research. Much of the social sciences research also tends to be "hand maiden" research focused on the implementation of biomedical solutions.

    CONCLUSION: There is little evidence that scientists pay any attention to the complex social, cultural, biological, and environmental dynamic involved in human pathogenesis. There is little investigator driven social science and a poor presence of interdisciplinary science. The research needs more sophisticated funders and priority setters who are not beguiled by uncritical biomedical promises.

  13. Ngow HA, Wan Khairina WM
    Pathol Oncol Res, 2011 Sep;17(3):771-4.
    PMID: 21213128 DOI: 10.1007/s12253-010-9328-9
    A 15 year-old adolescent was referred with 2 month history of worsening of breathlessness and haemoptysis. He also reported constitutional symptoms of fever, poor appetite and weight loss. The chest roentgenogram showed a massive right pleural effusion with apparent cardiomegaly. The cardiac silhouette over the right heart border was obliterated and the mediastinum was widened. Computed tomogram of the thorax showed a bulky heterogeneous mass in the right lung with extension to the heart. Subsequent CT guided lung biopsy revealed Primitive Neuroectodermal tumour (PNET). Here, we illustrate the clinical course of an aggressive pulmonary PNET with lethal cardiac metastasis.
    MeSH terms: Adolescent; Heart Neoplasms/secondary*; Heart Neoplasms/therapy; Humans; Lung Neoplasms/pathology*; Lung Neoplasms/therapy; Male; Radiography, Thoracic; Tomography, X-Ray Computed; Fatal Outcome; Neuroectodermal Tumors, Primitive/pathology*; Neuroectodermal Tumors, Primitive/therapy
  14. Nurulaini H, Wong TW
    J Pharm Sci, 2011 Jun;100(6):2248-57.
    PMID: 21213311 DOI: 10.1002/jps.22459
    Conventional alginate pellets underwent rapid drug dissolution and loss of multiparticulate characteristics such as aggregation in acidic medium, thereby promoting oral dose dumping. This study aimed to design sustained-release dispersible alginate pellets through rapid in situ matrix dispersion and cross-linking by calcium salts during dissolution. Pellets made of alginate and calcium salts were prepared using a solvent-free melt pelletization technique that prevented reaction between processing materials during agglomeration and allowed such a reaction to occur only in dissolution phase. Drug release was remarkably retarded in acidic medium when pellets were formulated with water-soluble calcium acetate instead of acid-soluble calcium carbonate. Different from calcium salt-free and calcium carbonate-loaded matrices that aggregated or underwent gradual erosion, rapid in situ solvation of calcium acetate in pellets during dissolution resulted in burst of gas bubbles, fast pellet breakup, and dispersion. The dispersed fragments, though exhibiting a larger specific surface area for drug dissolution than intact matrix, were rapidly cross-linked by Ca(2+) from calcium acetate and had drug release retarded till a change in medium pH from 1.2 to 6.8. Being dispersible and pH-dependent in drug dissolution, these pellets are useful as multiparticulate intestinal-specific drug carrier without exhibiting dose dumping tendency of a "single-unit-like" system via pellet aggregation.
    MeSH terms: Acetates/chemistry; Alginates/chemistry*; Calcium Carbonate/chemistry; Calorimetry, Differential Scanning; Cross-Linking Reagents/chemistry*; Delayed-Action Preparations; Drug Carriers/chemistry*; Gastric Juice/chemistry; Hexuronic Acids/chemistry; Humans; Hydrogen-Ion Concentration; Intestinal Secretions/chemistry; Intestines*; Kinetics; Microscopy, Electron, Scanning; Models, Biological; Particle Size; Solubility; Surface Properties; Technology, Pharmaceutical/methods*; Drug Design*; Spectroscopy, Fourier Transform Infrared; Calcium Compounds/chemistry; Glucuronic Acid/chemistry; Hydrophobic and Hydrophilic Interactions
  15. Zakaria ZA, Mohamed AM, Jamil NS, Rofiee MS, Hussain MK, Sulaiman MR, et al.
    Am J Chin Med, 2011;39(1):183-200.
    PMID: 21213408
    The in vitro antiproliferative and antioxidant activities of the aqueous, chloroform and methanol extracts of Muntingia calabura leaves were determined in the present study. Assessed using the 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay, the aqueous and methanol extracts of M. calabura inhibited the proliferation of MCF-7, HeLa, HT-29, HL-60 and K-562 cancer cells while the chloroform extract only inhibited the proliferation of MCF-7, HeLa, HL-60 and K-562 cancer cells. Interestingly, all extracts of M. calabura, which failed to inhibit the MDA-MB-231 cells proliferation, did not inhibit the proliferation of 3T3 (normal) cells, indicating its safety. All extracts (20, 100 and 500 μg/ml) were found to possess antioxidant activity when tested using the DPPH radical scavenging and superoxide scavenging assays with the methanol, followed by the aqueous and chloroform, extract exhibiting the highest antioxidant activity in both assays. The total phenolic content for the aqueous, methanol and chloroform extracts were 2970.4 ± 6.6, 1279.9 ± 6.1 and 2978.1 ± 4.3 mg/100 g gallic acid, respectively. In conclusion, the M. calabura leaves possess potential antiproliferative and antioxidant activities that could be attributed to its high content of phenolic compounds, and thus, needs to be further explored.
    MeSH terms: Antineoplastic Agents, Phytogenic/isolation & purification; Antineoplastic Agents, Phytogenic/pharmacology; Antineoplastic Agents, Phytogenic/therapeutic use*; Antioxidants/pharmacology; Antioxidants/therapeutic use*; Biphenyl Compounds/metabolism; HeLa Cells; Humans; Phytotherapy; Neoplasms/drug therapy*; Phenols/isolation & purification; Phenols/pharmacology; Phenols/therapeutic use*; Picrates/metabolism; Plant Extracts/pharmacology; Plant Extracts/therapeutic use*; Plant Leaves; Elaeocarpaceae/chemistry*; Cell Line, Tumor; Cell Proliferation/drug effects*
  16. Mohamad AS, Akhtar MN, Khalivulla SI, Perimal EK, Khalid MH, Ong HM, et al.
    Basic Clin Pharmacol Toxicol, 2011 Jun;108(6):400-5.
    PMID: 21214864 DOI: 10.1111/j.1742-7843.2010.00670.x
    The possible mechanisms of action in the antinociceptive activity induced by systemic administration (intraperitoneal, i.p.) of flavokawin B (FKB) were analysed using chemical models of nociception in mice. It was demonstrated that i.p. administration of FKB to the mice at 0.3, 1.0, 3.0 and 10 mg/kg produced significant dose-related reduction in the number of abdominal constrictions. The antinociception induced by FKB in the acetic acid test was significantly attenuated by i.p. pre-treatment of mice with L-arginine, the substrate for nitric oxide synthase or glibenclamide, the ATP-sensitive K(+) channel inhibitor, but was enhanced by methylene blue, the non-specific guanylyl cyclase inhibitor. FKB also produced dose-dependent inhibition of licking response caused by intraplantar injection of phorbol 12-myristate 13-acetate, a protein kinase C activator (PKC). Together, these data indicate that the NO/cyclic guanosine monophosphate/PKC/ATP-sensitive K(+) channel pathway possibly participated in the antinociceptive action induced by FKB.
    MeSH terms: Analgesics/chemical synthesis; Analgesics/pharmacology*; Animals; Arginine/metabolism; Arginine/pharmacology; Dose-Response Relationship, Drug; Enzyme Inhibitors/pharmacology; Flavonoids/chemical synthesis; Flavonoids/pharmacology*; Glyburide/pharmacology; Cyclic GMP/physiology*; Hypoglycemic Agents/pharmacology; Male; Methylene Blue/pharmacology; Mice, Inbred ICR; Nitric Oxide/physiology*; Pain/chemically induced; Pain/drug therapy; Protein Kinase C/physiology*; Potassium Channels/physiology*; Signal Transduction/drug effects; Nitric Oxide Synthase/metabolism; Mice
  17. Amran AA, Zakaria Z, Othman F, Das S, Al-Mekhlafi HM, Nordin NA
    Lipids Health Dis, 2011 Jan 09;10:2.
    PMID: 21214952 DOI: 10.1186/1476-511X-10-2
    BACKGROUND: Inflammation process plays an important role in the development of atherosclerosis. Hypercholesterolemia is one of the major risk factors for atherosclerosis. The present study aimed to evaluate the effect of aqueous extract of Piper sarmentosum (P.s) on inflammatory markers like vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), and C-reactive protein (CRP).

    METHODS: Forty two male New Zealand white rabbits were divided equally into seven groups; (i) C- control group fed normal rabbit chow (ii) CH- cholesterol diet (1%cholesterol) (iii) X1- 1% cholesterol with water extract of P.s (62.5 mg/kg) (iv) X2- 1% cholesterol with water extract of P.s (125 mg/kg (v) X3- 1% cholesterol with water extract of P.s (250 mg/kg) (vi) X4- 1% cholesterol with water extract of P.s (500 mg/kg) and (vii) SMV group fed with 1% cholesterol supplemented with simvistatin drug (1.2 mg/kg). All animals were treated for 10 weeks. Blood serum was taken for observing the inflammatory markers at the beginning and end of the experiment.

    RESULTS: Rabbits fed with 1% cholesterol diet (CH) showed significant increase in the level of VCAM-1, ICAM-1 and CRP compared to the C group. The levels of VCAM-1, ICAM-1 and CRP in the 1% cholesterol group and supplemented with P.s (500 mg/kg) were significantly reduced compared to the cholesterol group. Similar results were also reported with simvistatin group.

    CONCLUSION: These results suggest that the supplementation of Piper sarmentosum extract could inhibit inflammatory markers which in turn could prevent atherosclerosis.

    MeSH terms: Animals; Antioxidants/pharmacology; Antioxidants/therapeutic use*; Antioxidants/chemistry; C-Reactive Protein/metabolism*; Cholesterol, Dietary/administration & dosage; Hypercholesterolemia/drug therapy; Hypercholesterolemia/metabolism; Male; Phytotherapy; Plant Extracts/pharmacology; Plant Extracts/therapeutic use*; Plant Extracts/chemistry; Rabbits; Plant Leaves/chemistry; Intercellular Adhesion Molecule-1/blood*; Vascular Cell Adhesion Molecule-1/blood*; Piper/chemistry*
  18. Faruque MR, Islam MT, Misran N
    Med Eng Phys, 2011 Jun;33(5):646-52.
    PMID: 21216175 DOI: 10.1016/j.medengphy.2010.12.004
    The purpose of this paper is to calculate the specific absorption rate (SAR) reduction in a muscle cube with metamaterial attachment. The finite-difference time-domain (FDTD) method has been used to evaluate the SAR in a realistic anatomically based model of the muscle cube. In this paper, we have designed the single-negative metamaterials from a periodic arrangement of split ring resonators (SRRs). By properly designing the structural parameter of the SRRs, the effective medium parameter can be tuned negative at the 900 MHz and 1800 MHz bands. Numerical results concerning the SAR values in the muscle cube in the presence of resonators exhibit significant SAR reduction. These results can provide useful information when designing safety-compliant mobile communication equipment.
    MeSH terms: Absorption; Electromagnetic Fields*; Engineering*; Face; Humans; Muscles/cytology; Muscles/metabolism; Muscles/radiation effects*; Permeability/radiation effects; Skin; Time Factors; Phantoms, Imaging
  19. Voo WP, Ravindra P, Tey BT, Chan ES
    J Biosci Bioeng, 2011 Mar;111(3):294-9.
    PMID: 21216192 DOI: 10.1016/j.jbiosc.2010.11.010
    A comparative study on the stability and potential of alginate and pectin based beads for production of poultry probiotic cells using MRS medium in repeated batch fermentation was conducted. The bead cores, made of three types of materials, i.e., ca-alginate, ca-pectinate and ca-alginate/pectinate, were compared. The effect of single and double layer coatings using chitosan and core material, respectively, on the bead stability and cell production were also studied. The pectin based beads were found to be more stable than that of the alginate beads and their stability was further improved by coating with chitosan. The cell concentration in pectin based beads was comparable to that in the alginate beads. On the other hand, pectin based beads gave significantly lower cell concentration in the growth medium for the initial fermentation cycles when compared to the alginate beads. In conclusion, pectin was found to be potential encapsulation material for probiotic cell production owing to its stability and favourable microenvironment for cell growth.
    MeSH terms: Alginates/chemistry*; Animals; Culture Media; Fermentation*; Hexuronic Acids/chemistry; Lactobacillus/growth & development; Microspheres*; Pectins/chemistry*; Poultry; Cells, Immobilized; Probiotics*; Glucuronic Acid/chemistry; Chitosan/metabolism
  20. Wells K, Lakim MB, Beaucournu JC
    Med Vet Entomol, 2011 Sep;25(3):311-9.
    PMID: 21219372 DOI: 10.1111/j.1365-2915.2010.00940.x
    The diversity of ectoparasites in Southeast Asia and flea-host associations remain largely understudied. We explore specialization and interaction patterns of fleas infesting non-volant small mammals in Bornean rainforests, using material from a field survey carried out in two montane localities in northwestern Borneo (Sabah, Malaysia) and from a literature database of all available interactions in both lowland and montane forests. A total of 234 flea individuals collected during our field survey resulted in an interaction network of eight flea species on seven live-captured small mammal species. The interaction network from all compiled studies currently includes 15 flea species and 16 small mammal species. Host specificity and niche partitioning of fleas infesting diurnal treeshrews and squirrels were low, with little difference in specialization among taxa, but host specificity in lowland forests was found to be higher than in montane forests. By contrast, Sigmactenus alticola (Siphonaptera: Leptopsyllidae) exhibited low host specificity by infesting various montane and lowland nocturnal rats. However, this species exhibited low niche partitioning as it was the only commonly recorded flea from rats on Borneo. Overall complementary specialization was of intermediate intensity for both networks and differed significantly from random association; this has important implications for specific interactions that are also relevant to the potential spread of vector-borne diseases.
    MeSH terms: Animals; Borneo; Ectoparasitic Infestations/parasitology; Ectoparasitic Infestations/veterinary*; Siphonaptera/physiology*; Host-Parasite Interactions; Mammals/parasitology*; Species Specificity; Trees*; Ecosystem*
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