Browse publications by year: 2016

  1. Ahmed RH, Huri HZ, Al-Hamodi Z, Salem SD, Al-Absi B, Muniandy S
    PLoS One, 2016;11(4):e0154369.
    PMID: 27111895 DOI: 10.1371/journal.pone.0154369
    BACKGROUND: Genetic polymorphisms of the Dipeptidyl Peptidase 4 (DPP4) gene may play a role in the etiology of type 2 diabetes mellitus (T2DM). This study aimed to investigate the possible association of single nucleotide polymorphisms (SNPs) of the DPP4 gene in Malaysian subjects with T2DM and evaluated whether they had an effect on the serum levels of soluble dipeptidyl peptidase 4 (sDPP-IV).

    METHOD: Ten DPP4 SNPs were genotyped by TaqMan genotyping assays in 314 subjects with T2DM and 235 controls. Of these, 71 metabolic syndrome (MetS) subjects were excluded from subsequent analysis. The odds ratios (ORs) and their 95% confidence interval (CIs) were calculated using multiple logistic regression for the association between the SNPs of DPP4 and T2DM. In addition, the serum levels of sDPP-IV were investigated to evaluate the association of the SNPs of DPP4 with the sDPP-IV levels.

    RESULTS: Dominant, recessive, and additive genetic models were employed to test the association of DPP4 polymorphisms with T2DM, after adjusting for age, race, gender and BMI. The rs12617656 was associated with T2DM in Malaysian subjects in the recessive genetic model (OR = 1.98, p = 0.006), dominant model (OR = 1.95, p = 0.008), and additive model (OR = 1.63, p = 0.001). This association was more pronounced among Malaysian Indians, recessive (OR = 3.21, p = 0.019), dominant OR = 3.72, p = 0.003) and additive model (OR = 2.29, p = 0.0009). The additive genetic model showed that DPP4 rs4664443 and rs7633162 polymorphisms were associated with T2DM (OR = 1.53, p = 0.039), and (OR = 1.42, p = 0.020), respectively. In addition, the rs4664443 G>A polymorphism was associated with increased sDPP-IV levels (p = 0.042) in T2DM subjects.

    CONCLUSIONS: DPP4 polymorphisms were associated with T2DM in Malaysian subjects, and linked to variations in sDPP-IV levels. In addition, these associations were more pronounced among Malaysian Indian subjects.

    MeSH terms: Adolescent; Adult; Aged; Alleles; Child; Child, Preschool; Diabetes Mellitus, Type 2/ethnology*; Diabetes Mellitus, Type 2/genetics*; Female; Genotype; Graves Disease/genetics; Graves Disease/epidemiology; Haplotypes; Humans; Malaysia; Male; Middle Aged; Polymorphism, Genetic; Dipeptidyl Peptidase 4/genetics*; Polymorphism, Single Nucleotide*; Asian Continental Ancestry Group; Hashimoto Disease/genetics; Hashimoto Disease/epidemiology; Young Adult; CTLA-4 Antigen/genetics
  2. Kwong QB, Teh CK, Ong AL, Heng HY, Lee HL, Mohamed M, et al.
    Mol Plant, 2016 Aug 01;9(8):1132-1141.
    PMID: 27112659 DOI: 10.1016/j.molp.2016.04.010
    High-density single nucleotide polymorphism (SNP) genotyping arrays are powerful tools that can measure the level of genetic polymorphism within a population. To develop a whole-genome SNP array for oil palms, SNP discovery was performed using deep resequencing of eight libraries derived from 132 Elaeis guineensis and Elaeis oleifera palms belonging to 59 origins, resulting in the discovery of >3 million putative SNPs. After SNP filtering, the Illumina OP200K custom array was built with 170 860 successful probes. Phenetic clustering analysis revealed that the array could distinguish between palms of different origins in a way consistent with pedigree records. Genome-wide linkage disequilibrium declined more slowly for the commercial populations (ranging from 120 kb at r(2) = 0.43 to 146 kb at r(2) = 0.50) when compared with the semi-wild populations (19.5 kb at r(2) = 0.22). Genetic fixation mapping comparing the semi-wild and commercial population identified 321 selective sweeps. A genome-wide association study (GWAS) detected a significant peak on chromosome 2 associated with the polygenic component of the shell thickness trait (based on the trait shell-to-fruit; S/F %) in tenera palms. Testing of a genomic selection model on the same trait resulted in good prediction accuracy (r = 0.65) with 42% of the S/F % variation explained. The first high-density SNP genotyping array for oil palm has been developed and shown to be robust for use in genetic studies and with potential for developing early trait prediction to shorten the oil palm breeding cycle.
    MeSH terms: Chromosome Mapping; Genotype; Linkage Disequilibrium/genetics; Genome, Plant/genetics; Polymorphism, Single Nucleotide/genetics*; Arecaceae/genetics*; Quantitative Trait Loci/genetics*; Genome-Wide Association Study
  3. Sanyang ML, Sapuan SM, Jawaid M, Ishak MR, Sahari J
    Carbohydr Polym, 2016 08 01;146:36-45.
    PMID: 27112848 DOI: 10.1016/j.carbpol.2016.03.051
    The development and characterization of environmentally friendly bilayer films from sugar palm starch (SPS) and poly(lactic acid) (PLA) were conducted in this study. The SPS-PLA bilayer films and their individual components were characterized for their physical, mechanical, thermal and water barrier properties. Addition of 50% PLA layer onto 50% SPS layer (SPS50-PLA50) increased the tensile strength of neat SPS film from 7.74 to 13.65MPa but reduced their elongation at break from 46.66 to 15.53%. The incorporation of PLA layer significantly reduced the water vapor permeability as well as the water uptake and solubility of bilayer films which was attributed to the hydrophobic characteristic of the PLA layer. Furthermore, scanning electron microscopy (SEM) image of SPS50-PLA50 revealed lack of strong interfacial adhesion between the SPS and PLA. Overall, the incorporation of PLA layer onto SPS films enhances the suitability of SPS based films for food packaging.
    MeSH terms: Polyesters/chemistry*; Polymers; Starch/chemistry*; Tensile Strength; Food Packaging*; Arecaceae/chemistry*
  4. Mohamed MA, W Salleh WN, Jaafar J, Mohd Hir ZA, Rosmi MS, Abd Mutalib M, et al.
    Carbohydr Polym, 2016 08 01;146:166-73.
    PMID: 27112862 DOI: 10.1016/j.carbpol.2016.03.050
    Visible light driven C-doped mesoporous TiO2 (C-MTiO2) nanorods have been successfully synthesized through green, low cost, and facile approach by sol-gel bio-templating method using regenerated cellulose membrane (RCM) as nanoreactor. In this study, RCM was also responsible to provide in-situ carbon sources for resultant C-MTiO2 nanorods in acidified sol at low temperatures. The composition, crystallinity, surface area, morphological structure, and optical properties of C-MTiO2 nanorods, respectively, had been characterized using FTIR, XRD, N2 adsorption/desorption, TEM, UV-vis-NIR, and XPS spectroscopy. The results suggested that the growth of C-MTiO2 nanorods was promoted by the strong interaction between the hydroxyl groups of RCMs and titanium ion. Optical and XPS analysis confirmed that carbon presence in TiO2 nanorods were responsible for band-gap narrowing, which improved the visible light absorption capability. Photocatalytic activity measurements exhibited the capability of C-MTiO2 nanorods in degradation of methyl orange in aqueous solution, with 96.6% degradation percentage under visible light irradiation.
    MeSH terms: Catalysis; Cellulose/chemistry*; Light*; Membranes, Artificial*; Titanium/chemistry*
  5. Mokhtar AS, Braima KA, Peng Chin H, Jeffery J, Mohd Zain SN, Rohela M, et al.
    J Med Entomol, 2016 Apr 25.
    PMID: 27113101 DOI: 10.1093/jme/tjw014
    We report a case of human intestinal myiasis in a 41-yr-old female patient presented at a clinic in Seri Kembangan, Selangor, Malaysia. Larvae passed out in the patient's feces were sent to the Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. DNA barcoding confirmed the second case of intestinal myiasis in Malaysia involving the larvae of Clogmia albipunctatus (Duckhouse) (Diptera: Psychodidae). We review reported cases of myiasis and discuss the present case of intestinal myiasis in an urban patient.
    MeSH terms: Ambulatory Care Facilities; Animals; DNA; Faculty; Feces; Female; Humans; Larva; Malaysia; Myiasis; Psychodidae; DNA Barcoding, Taxonomic
  6. Moriya S, Soga T, Wong DW, Parhar IS
    Neurosci Lett, 2016 May 27;622:67-71.
    PMID: 27113202 DOI: 10.1016/j.neulet.2016.04.052
    The decrease in serotonergic neurotransmission during aging can increase the risk of neuropsychiatric diseases such as depression in elderly population and decline the reproductive system. Therefore, it is important to understand the age-associated molecular mechanisms of brain aging. In this study, the effect of aging and chronic escitalopram (antidepressant) treatment to admit mice was investigated by comparing transcriptomes in the preoptic area (POA) which is a key nucleus for reproduction. In the mid-aged brain, the immune system-related genes were increased and hormone response-related genes were decreased. In the escitalopram treated brains, transcription-, granule cell proliferation- and vasoconstriction-related genes were increased and olfactory receptors were decreased. Since homeostasis and neuroprotection-related genes were altered in both of mid-age and escitalopram treatment, these genes could be important for serotonin related physiologies in the POA.
    MeSH terms: Age Factors; Animals; Male; Mice, Inbred C57BL; Preoptic Area/drug effects*; Preoptic Area/metabolism; Serotonin/metabolism; Citalopram/pharmacology*; Serotonin Uptake Inhibitors/pharmacology*; Transcriptome*
  7. Bamrungsawad N, Upakdee N, Pratoomsoot C, Sruamsiri R, Dilokthornsakul P, Dechanont S, et al.
    Clin Drug Investig, 2016 Jul;36(7):557-66.
    PMID: 27113227 DOI: 10.1007/s40261-016-0401-3
    Intravenous immunoglobulin (IVIG) has been recommended for steroid-resistant chronic inflammatory demyelinating polyradiculoneuropathy (CIDP). The treatment, however, is very costly to healthcare system, and there remains no evidence of its economic justifiability. This study aimed to conduct an economic evaluation (EE) of IVIG plus corticosteroids in steroid-resistant CIDP in Thailand.
  8. Saba L, Than JC, Noor NM, Rijal OM, Kassim RM, Yunus A, et al.
    J Med Syst, 2016 Jun;40(6):142.
    PMID: 27114353 DOI: 10.1007/s10916-016-0504-7
    Human interaction has become almost mandatory for an automated medical system wishing to be accepted by clinical regulatory agencies such as Food and Drug Administration. Since this interaction causes variability in the gathered data, the inter-observer and intra-observer variability must be analyzed in order to validate the accuracy of the system. This study focuses on the variability from different observers that interact with an automated lung delineation system that relies on human interaction in the form of delineation of the lung borders. The database consists of High Resolution Computed Tomography (HRCT): 15 normal and 81 diseased patients' images taken retrospectively at five levels per patient. Three observers manually delineated the lungs borders independently and using software called ImgTracer™ (AtheroPoint™, Roseville, CA, USA) to delineate the lung boundaries in all five levels of 3-D lung volume. The three observers consisted of Observer-1: lesser experienced novice tracer who is a resident in radiology under the guidance of radiologist, whereas Observer-2 and Observer-3 are lung image scientists trained by lung radiologist and biomedical imaging scientist and experts. The inter-observer variability can be shown by comparing each observer's tracings to the automated delineation and also by comparing each manual tracing of the observers with one another. The normality of the tracings was tested using D'Agostino-Pearson test and all observers tracings showed a normal P-value higher than 0.05. The analysis of variance (ANOVA) test between three observers and automated showed a P-value higher than 0.89 and 0.81 for the right lung (RL) and left lung (LL), respectively. The performance of the automated system was evaluated using Dice Similarity Coefficient (DSC), Jaccard Index (JI) and Hausdorff (HD) Distance measures. Although, Observer-1 has lesser experience compared to Obsever-2 and Obsever-3, the Observer Deterioration Factor (ODF) shows that Observer-1 has less than 10% difference compared to the other two, which is under acceptable range as per our analysis. To compare between observers, this study used regression plots, Bland-Altman plots, two tailed T-test, Mann-Whiney, Chi-Squared tests which showed the following P-values for RL and LL: (i) Observer-1 and Observer-3 were: 0.55, 0.48, 0.29 for RL and 0.55, 0.59, 0.29 for LL; (ii) Observer-1 and Observer-2 were: 0.57, 0.50, 0.29 for RL and 0.54, 0.59, 0.29 for LL; (iii) Observer-2 and Observer-3 were: 0.98, 0.99, 0.29 for RL and 0.99, 0.99, 0.29 for LL. Further, CC and R-squared coefficients were computed between observers which came out to be 0.9 for RL and LL. All three observers however manage to show the feature that diseased lungs are smaller than normal lungs in terms of area.
    MeSH terms: Adolescent; Adult; Aged; Aged, 80 and over; Female; Humans; Image Interpretation, Computer-Assisted*; Lung Neoplasms/diagnosis*; Lung Neoplasms/pathology; Male; Middle Aged; Tomography, X-Ray Computed/methods*; United States; Observer Variation*; Young Adult
  9. Tiruvayipati S, Bhassu S
    Gut Pathog, 2016;8:15.
    PMID: 27114742 DOI: 10.1186/s13099-016-0097-1
    Macrobrachium rosenbergii is well-known as the giant freshwater prawn, and is a commercially significant source of seafood. Its production can be affected by various bacterial contaminations. Among which, the genus Vibrio shows a higher prevalence in aquatic organisms, especially M. rosenbergii, causing food-borne illnesses. Vibrio parahaemolyticus, a species of Vibrio is reported as the main causative of the early mortality syndrome. Vibrio parahaemolyticus infection in M. rosenbergii was studied previously in relation to the prawn's differentially expressed immune genes. In the current review, we will discuss the growth conditions for both V. parahaemolyticus and M. rosenbergii and highlight the role of magnesium in common, which need to be fully understood. Till date, there has not been much research on this aspect of magnesium. We postulate a model that screens a magnesium-dependent pathway which probably might take effect in connection with N-acetylglucosamine binding protein and chitin from V. parahaemolyticus and M. rosenbergii, respectively. Further studies on magnesium as an environment for V. parahaemolyticus and M. rosenbergii interaction studies will provide seafood industry with completely new strategies to employ and to avoid seafood related contaminations.
    MeSH terms: Acetylglucosamine; Animals; Carrier Proteins; Chitin; Foodborne Diseases; Fresh Water; Magnesium; Vibrio Infections; Vibrio parahaemolyticus; Prevalence; Palaemonidae; Aquatic Organisms
  10. Wong FN, Chua KH, Kuppusamy UR, Wong CM, Lim SK, Tan JA
    PeerJ, 2016;4:e1908.
    PMID: 27114872 DOI: 10.7717/peerj.1908
    Chronic kidney disease (CKD) is a condition associated with progressive loss of kidney function and kidney damage. The two common causes of CKD are diabetes mellitus and hypertension. Other causes of CKD also include polycystic kidney disease, obstructive uropathy and primary glomerulonephritis. The receptor for advanced glycation end-products (RAGE) is a multi-ligand cell surface receptor of the immunoglobulin superfamily and it has been associated with kidney disease in both non-diabetic and diabetic patients. Presently, data on the association between RAGE polymorphisms and CKD in the Malaysian population is limited, while numerous studies have reported associations of RAGE polymorphisms with diabetic complications in other populations. The present study aims to explore the possibility of using RAGE polymorphisms as candidate markers of CKD in Malaysian population by using association analysis.
    MeSH terms: Diabetes Mellitus; Glomerulonephritis; Humans; Hypertension; Immunoglobulins; Polycystic Kidney Diseases; Diabetes Complications; Renal Insufficiency, Chronic
  11. Lal TM, Sano M, Hatai K, Ransangan J
    Genom Data, 2016 Jun;8:37-8.
    PMID: 27114905 DOI: 10.1016/j.gdata.2016.03.002
    This paper describes the complete sequence of a giant lytic marine myophage, Vibrio phage ValKK3 that is specific to Vibrio alginolyticus ATCC(®) 17749™. Vibrio phage ValKK3 was subjected to whole genome sequencing on MiSeq sequencing platform and annotated using Blast2Go. The complete sequence of ValKK3 genome was deposited in DBBJ/EMBL/GenBank under accession number KP671755.
  12. Khan MJ, Chelliah S, Haron MS
    Iran J Public Health, 2016 Feb;45(2):134-45.
    PMID: 27114978
    BACKGROUND: Role of information source, perceived benefits and risks, and destination image has significantly been examined in travel and tourism literature; however, in medical tourism it is yet to be examined thoroughly. The concept discussed in this article is drawn form well established models in tourism literature.

    METHODS: The purpose of this research was to identify the source of information, travel benefits and perceived risks related to movement of international patients and develop a conceptual model based on well-established theory. Thorough database search (Science Direct, utmj.org, nih.gov, nchu.edu.tw, palgrave-journals, medretreat, Biomedcentral) was performed to fulfill the objectives of the study.

    RESULTS: International patients always concern about benefits and risks related to travel. These benefits and risks form images of destination in the minds of international patients. Different sources of information make international patients acquaint about the associated benefits and risks, which later leads to development of intention to visit. This conceptual paper helps in establishing model for decision-making process of international patients in developing visit intention.

    CONCLUSION: Ample amount of literature is available detailing different factors involved in travel decision making of international patients; however literature explaining relationship between these factors is scarce.

    MeSH terms: Decision Making; Humans; Perception; Research; Risk Assessment; Intention; Medical Tourism
  13. Dieng H, Tan Yusop NS, Kamal NN, Ahmad AH, Ghani IA, Abang F, et al.
    J Agric Food Chem, 2016 May 11;64(18):3485-91.
    PMID: 27115536 DOI: 10.1021/acs.jafc.6b01157
    Dengue mosquitoes are evolving into a broader global public health menace, with relentless outbreaks and the rise in number of Zika virus disease cases as reminders of the continued hazard associated with Aedes vectors. The use of chemical insecticides-the principal strategy against mosquito vectors-has been greatly impeded due to the development of insecticide resistance and the shrinking spectrum of effective agents. Therefore, there is a pressing need for new chemistries for vector control. Tea contains hundreds of chemicals, and its waste, which has become a growing global environmental problem, is almost as rich in toxicants as green leaves. This paper presents the toxic and sublethal effects of different crude extracts of tea on Aedes albopictus. The survival rates of larvae exposed to tea extracts, especially fresh tea extract (FTE), were markedly lower than those in the control treatment group. In addition to this immediate toxicity against different developmental stages, the extracts tested caused a broad range of sublethal effects. The developmental time was clearly longer in containers with tea, especially in those with young larvae (YL) and FTE. Among the survivors, pupation success was reduced in containers with tea, which also produced low adult emergence rates with increasing tea concentration. The production of eggs tended to be reduced in females derived from the tea treatment groups. These indirect effects of tea extracts on Ae. albopictus exhibited different patterns according to the exposed larval stage. Taken together, these findings indicate that tea and its waste affect most key components of Ae. albopictus vectorial capacity and may be useful for dengue control. Reusing tea waste in vector control could also be a practical solution to the problems associated with its pollution.
    MeSH terms: Aedes/drug effects*; Aedes/growth & development; Aedes/physiology; Animals; Dengue/transmission; Female; Insect Vectors/drug effects*; Insect Vectors/growth & development; Insect Vectors/physiology; Insecticides/pharmacology*; Larva/drug effects; Larva/growth & development; Male; Mosquito Control; Oviposition/drug effects; Plant Extracts/pharmacology*; Waste Products/analysis; Camellia sinensis/chemistry*
  14. Masran R, Zanirun Z, Bahrin EK, Ibrahim MF, Lai Yee P, Abd-Aziz S
    Appl Microbiol Biotechnol, 2016 Jun;100(12):5231-46.
    PMID: 27115758 DOI: 10.1007/s00253-016-7545-1
    Abundant lignocellulosic biomass from various industries provides a great potential feedstock for the production of value-added products such as biofuel, animal feed, and paper pulping. However, low yield of sugar obtained from lignocellulosic hydrolysate is usually due to the presence of lignin that acts as a protective barrier for cellulose and thus restricts the accessibility of the enzyme to work on the cellulosic component. This review focuses on the significance of biological pretreatment specifically using ligninolytic enzymes as an alternative method apart from the conventional physical and chemical pretreatment. Different modes of biological pretreatment are discussed in this paper which is based on (i) fungal pretreatment where fungi mycelia colonise and directly attack the substrate by releasing ligninolytic enzymes and (ii) enzymatic pretreatment using ligninolytic enzymes to counter the drawbacks of fungal pretreatment. This review also discusses the important factors of biological pretreatment using ligninolytic enzymes such as nature of the lignocellulosic biomass, pH, temperature, presence of mediator, oxygen, and surfactant during the biodelignification process.
    MeSH terms: Animals; Animal Feed; Cellulose/metabolism; Fungi/metabolism; Hydrolysis; Lignin/metabolism*; Peroxidases/metabolism; Surface-Active Agents; Biomass*; Laccase/metabolism; Biofuels
  15. Rahman MM, Khadijah WE, Abdullah RB
    Trop Anim Health Prod, 2016 Aug;48(6):1287-90.
    PMID: 27116608 DOI: 10.1007/s11250-016-1065-y
    Twelve Jermasia kids were individually housed in pens to study the effects of soywaste on growth performance and carcass characteristics and to compare such effects with commercial pellet. Kids were divided into a pellet group and a soywaste group, including six kids (3 males and 3 females) in each group. Pellet or soywaste was offered to kids at a rate of 2.0 % dry matter (DM) of body weight/day in addition to Napier grass ad libitum. In last 10 days of experiment, kids were housed in metabolism crates for faeces collection. At the end of the experiment, three males from each group were slaughtered. Kids fed soywaste diet consumed more grass and neutral detergent fibre (NDF) than those fed pellet. The same trend was found for the digestibilities of DM, organic matter (OM) and NDF. Conversely, kids fed soywaste diet consumed less soywaste supplement than kids fed pellet. No treatment effects were observed on total intakes of DM, OM and crude protein (CP) including CP digestibility. Similarly, no effects were found on carcass and non-carcass components, except for lean, lean to fat ratio and kidney weight which were higher for kids fed soywaste diet. Results indicate that soywaste is effective as a feed for growing kids.
    MeSH terms: Animals; Animal Feed*; Animal Nutritional Physiological Phenomena; Body Composition; Diet/veterinary*; Dietary Fiber/metabolism; Digestion; Female; Goats/physiology*; Male; Soybeans*; Weaning
  16. Selvanayagam VS, Riek S, DE Rugy A, Carroll TJ
    Med Sci Sports Exerc, 2016 09;48(9):1835-46.
    PMID: 27116648 DOI: 10.1249/MSS.0000000000000956
    PURPOSE: Goal-directed movements tend to resemble the characteristics of previously executed actions. Here we investigated whether a single bout of strength training, which typically involves stereotyped actions requiring strong neural drive, can bias subsequent aiming behavior toward the direction of trained forces.

    METHODS: In experiment 1 (n = 10), we tested the direction of force exerted in an isometric aiming task before and after 40 repetitions of 2-s maximal-force ballistic contractions toward a single directional target. In experiment 2 (n = 12), each participant completed three training conditions in a counterbalanced crossover design. In two conditions, both the aiming task and the training were conducted in the same (neutral) forearm posture. In one of these conditions, the training involved weak forces to determine whether the level of neural drive during training influences the degree of bias. In the third condition, high-force training contractions were performed in a 90° pronated forearm posture, whereas the low-force aiming task was performed in a neutral forearm posture. This dissociated the extrinsic training direction from the pulling direction of the trained muscles during the aiming task.

    RESULTS: In experiment 1, we found that aiming direction was biased toward the training direction across a large area of the work space (approximately ±135°; tested for 16 targets spaced 22.5° apart), whereas in experiment 2, we found systematic bias in aiming toward the training direction defined in extrinsic space, but only immediately after high-force contractions.

    CONCLUSION: Our findings suggest that bias effects of training involving strong neural drive generalize broadly to untrained movement directions and are expressed according to extrinsic rather than muscle-based coordinates.

    MeSH terms: Adult; Female; Forearm/physiology; Goals*; Humans; Isometric Contraction; Male; Nervous System Physiological Phenomena*; Posture; Resistance Training*; Young Adult
  17. Zyoud SH, Daraghmeh DN, Mezyed DO, Khdeir RL, Sawafta MN, Ayaseh NA, et al.
    BMC Nephrol, 2016 04 27;17(1):44.
    PMID: 27117687 DOI: 10.1186/s12882-016-0257-z
    BACKGROUND: Haemodialysis (HD) is a life-sustaining treatment for patients with end-stage renal disease (ESRD). HD can bring about significant impairment in health-related quality of life (HRQOL) and outcomes. Therefore, we sought to describe the patterns of HRQOL and determine the independent factors associated with poor HRQOL in Palestinian patients on HD.

    METHODS: A multicenter cross-sectional study was performed from June 2014 to January 2015 using the EuroQOL-5 Dimensions instrument (EQ-5D-5L) for the assessment of HRQOL. ESRD patients undergoing HD in all dialysis centres in the West Bank of Palestine were approached and recruited for this study. Multiple linear regression was carried out to identify factors that were significantly associated with HRQOL.

    RESULTS: Two hundred and sixty-seven patients were participated in the current study giving response rate of 96 %. Overall, 139 (52.1 %) were male, and the mean ± standard deviation age was 53.3 ± 16.2 years. The reported HRQOL as measured by mean EQ-5D-5L index value and Euro QOL visual analogue scale (EQ-VAS) score was 0.37 ± 0.44 and 59.38 ± 45.39, respectively. There was a moderate positive correlation between the EQ-VAS and the EQ-5D-5L index value (r = 0.42, p 

    MeSH terms: Adult; Aged; Cross-Sectional Studies; Female; Renal Dialysis/psychology*; Renal Dialysis/trends; Humans; Kidney Failure, Chronic/epidemiology; Kidney Failure, Chronic/psychology*; Kidney Failure, Chronic/therapy*; Male; Middle Aged; Middle East/epidemiology; Pilot Projects; Quality of Life/psychology*; Arabs/psychology*
  18. Skau JK, Nordin AB, Cheah JC, Ali R, Zainal R, Aris T, et al.
    Trials, 2016;17(1):215.
    PMID: 27117703 DOI: 10.1186/s13063-016-1345-x
    Over the past two decades, the population of Malaysia has grown rapidly and the prevalence of diabetes mellitus in Malaysia has dramatically increased, along with the frequency of obesity, hyperlipidaemia and hypertension. Early-life influences play an important role in the development of non-communicable diseases. Indeed, maternal lifestyle and conditions such as gestational diabetes mellitus or obesity can affect the risk of diabetes in the next generation. Lifestyle changes can help to prevent the development of type 2 diabetes mellitus. This is a protocol for an unblinded, community-based, randomised controlled trial in two arms to evaluate the efficacy of a complex behavioural change intervention, combining motivational interviewing provided by a community health promoter and access to a habit formation mobile application, among young Malaysian women and their spouses prior to pregnancy.
    MeSH terms: Adult; Anthropometry; Clinical Protocols; Community Health Services*; Diabetes Mellitus, Type 2/etiology; Diabetes Mellitus, Type 2/prevention & control*; Female; Habits; Humans; Health Knowledge, Attitudes, Practice*; Malaysia; Male; Prediabetic State/etiology; Prediabetic State/prevention & control*; Research Design; Risk Factors; Time Factors; Health Behavior*; Multivariate Analysis; Chi-Square Distribution; Linear Models; Preconception Care/methods*; Treatment Outcome; Spouses/psychology; Risk Reduction Behavior; Young Adult; Motivational Interviewing*; Mobile Applications*; Healthy Lifestyle
  19. Couch FJ, Kuchenbaecker KB, Michailidou K, Mendoza-Fandino GA, Nord S, Lilyquist J, et al.
    Nat Commun, 2016 Apr 27;7:11375.
    PMID: 27117709 DOI: 10.1038/ncomms11375
    Common variants in 94 loci have been associated with breast cancer including 15 loci with genome-wide significant associations (P<5 × 10(-8)) with oestrogen receptor (ER)-negative breast cancer and BRCA1-associated breast cancer risk. In this study, to identify new ER-negative susceptibility loci, we performed a meta-analysis of 11 genome-wide association studies (GWAS) consisting of 4,939 ER-negative cases and 14,352 controls, combined with 7,333 ER-negative cases and 42,468 controls and 15,252 BRCA1 mutation carriers genotyped on the iCOGS array. We identify four previously unidentified loci including two loci at 13q22 near KLF5, a 2p23.2 locus near WDR43 and a 2q33 locus near PPIL3 that display genome-wide significant associations with ER-negative breast cancer. In addition, 19 known breast cancer risk loci have genome-wide significant associations and 40 had moderate associations (P<0.05) with ER-negative disease. Using functional and eQTL studies we implicate TRMT61B and WDR43 at 2p23.2 and PPIL3 at 2q33 in ER-negative breast cancer aetiology. All ER-negative loci combined account for ∼11% of familial relative risk for ER-negative disease and may contribute to improved ER-negative and BRCA1 breast cancer risk prediction.
    MeSH terms: Breast Neoplasms/genetics*; Chromosomes, Human, Pair 2/genetics; Female; Genotype; Heterozygote; Humans; Mutation; Receptors, Estrogen/genetics*; Risk Factors; tRNA Methyltransferases; BRCA1 Protein/genetics; Genetic Predisposition to Disease/genetics*; Polymorphism, Single Nucleotide; Cyclophilins/genetics; Genome-Wide Association Study/methods*
  20. Pirabbasi E, Shahar S, Manaf ZA, Rajab NF, Manap RA
    J Nutr Sci Vitaminol (Tokyo), 2016;62(1):54-61.
    PMID: 27117852 DOI: 10.3177/jnsv.62.54
    Antioxidant therapy has a potential to be introduced as therapeutic modality for chronic obstructive pulmonary disease (COPD) patients. This study aimed to determine the effect of antioxidant supplementation [ascorbic acid and N-Acetylcysteine (NAC)] on nutritional and antioxidant status in male COPD patients. A parallel and single blind randomised controlled clinical trial (RCT) was conducted at two medical centers in Kuala Lumpur, Malaysia. Seventy-nine subjects were recruited and randomly divided into four trial arms (i.e., NAC, vitamin C, NAC+vitamin C and control groups) for six mo. The primary outcome was changes in body mass index by estimating power of 90% and significance level of p<0.05. Repeated Measure ANOVA showed that there was a significant interaction effect on BMI (p=0.046) and carbohydrate intake (p=0.030), especially in the NAC group. Plasma glutathione (GSH) increased significantly in all intervention groups, especially in vitamin C (p=0.005). A single supplementation of NAC or vitamin C improved nutritional and antioxidant status of subjects.
    MeSH terms: Acetylcysteine/administration & dosage*; Adult; Aged; Antioxidants/administration & dosage*; Antioxidants/analysis*; Ascorbic Acid/administration & dosage*; Dietary Carbohydrates/administration & dosage; Glutathione/blood; Humans; Malaysia; Male; Middle Aged; Nutritional Status/drug effects*; Vitamin A/blood; Body Mass Index; Single-Blind Method; Treatment Outcome; Dietary Supplements; Pulmonary Disease, Chronic Obstructive/drug therapy*; Pulmonary Disease, Chronic Obstructive/physiopathology
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