Browse publications by year: 2016

  1. Lim SL, Mustapha NM, Goh YM, Bakar NA, Mohamed S
    Mol Cell Biochem, 2016 May;416(1-2):85-97.
    PMID: 27106908 DOI: 10.1007/s11010-016-2698-x
    Metastasized lung and liver cancers cause over 2 million deaths annually, and are amongst the top killer cancers worldwide. Morinda citrifolia (Noni) leaves are traditionally consumed as vegetables in the tropics. The macro and micro effects of M. citrifolia (Noni) leaves on metastasized lung cancer development in vitro and in vivo were compared with the FDA-approved anti-cancer drug Erlotinib. The extract inhibited the proliferation and induced apoptosis in A549 cells (IC50 = 23.47 μg/mL) and mouse Lewis (LL2) lung carcinoma cells (IC50 = 5.50 μg/mL) in vitro, arrested cancer cell cycle at G0/G1 phases and significantly increased caspase-3/-8 without changing caspase-9 levels. The extract showed no toxicity on normal MRC5 lung cells. Non-small-cell lung cancer (NSCLC) A549-induced BALB/c mice were fed with 150 and 300 mg/kg M. citrifolia leaf extract and compared with Erlotinib (50 mg/kg body weight) for 21 days. It significantly increased the pro-apoptotic TRP53 genes, downregulated the pro-tumourigenesis genes (BIRC5, JAK2/STAT3/STAT5A) in the mice tumours, significantly increased the anti-inflammatory IL4, IL10 and NR3C1 expression in the metastasized lung and hepatic cancer tissues and enhanced the NFE2L2-dependent antioxidant responses against oxidative injuries. The extract elevated serum neutrophils and reduced the red blood cells, haemoglobin, corpuscular volume and cell haemoglobin concentration in the lung cancer-induced mammal. It suppressed inflammation and oedema, and upregulated the endogenous antioxidant responses and apoptotic genes to suppress the cancer. The 300 mg/kg extract was more effective than the 50 mg/kg Erlotinib for most of the parameters measured.
    MeSH terms: Erlotinib Hydrochloride/pharmacology*; Animals; Anti-Inflammatory Agents/pharmacology*; Anti-Inflammatory Agents/chemistry; Antioxidants/pharmacology*; Antioxidants/chemistry; Humans; Lung Neoplasms/drug therapy*; Lung Neoplasms/metabolism; Lung Neoplasms/pathology; Male; Mice, Inbred BALB C; Neoplasm Metastasis; Plant Extracts/pharmacology*; Plant Extracts/chemistry; Gene Expression Regulation, Neoplastic/drug effects; Apoptosis/drug effects*; Plant Leaves/chemistry*; Xenograft Model Antitumor Assays; Morinda/chemistry*; Cell Line, Tumor; Mice
  2. Nuzaihan M N M, Hashim U, Md Arshad MK, Kasjoo SR, Rahman SF, Ruslinda AR, et al.
    Biosens Bioelectron, 2016 Sep 15;83:106-14.
    PMID: 27107147 DOI: 10.1016/j.bios.2016.04.033
    In this paper, a silicon nanowire biosensor with novel molecular gate control has been demonstrated for Deoxyribonucleic acid (DNA) detection related to dengue virus (DENV). The silicon nanowire was fabricated using the top-down nanolithography approach, through nanostructuring of silicon-on-insulator (SOI) layers achieved by combination of the electron-beam lithography (EBL), plasma dry etching and size reduction processes. The surface of the fabricated silicon nanowire was functionalized by means of a three-step procedure involving surface modification, DNA immobilization and hybridization. This procedure acts as a molecular gate control to establish the electrical detection for 27-mers base targets DENV DNA oligomer. The electrical detection is based on the changes in current, resistance and conductance of the sensor due to accumulation of negative charges added by the immobilized probe DNA and hybridized target DNA. The sensitivity of the silicon nanowire biosensors attained was 45.0µAM(-1), which shows a wide-range detection capability of the sensor with respect to DNA. The limit of detection (LOD) achieved was approximately 2.0fM. The demonstrated results show that the silicon nanowire has excellent properties for detection of DENV with outstanding repeatability and reproducibility performances.
  3. Grigg MJ, William T, Menon J, Barber BE, Wilkes CS, Rajahram GS, et al.
    Clin Infect Dis, 2016 Jun 01;62(11):1403-1411.
    PMID: 27107287 DOI: 10.1093/cid/ciw121
    BACKGROUND: Chloroquine (CQ)-resistant Plasmodium vivax is increasingly reported throughout southeast Asia. The efficacy of CQ and alternative artemisinin combination therapies (ACTs) for vivax malaria in Malaysia is unknown.

    METHODS: A randomized, controlled trial of CQ vs artesunate-mefloquine (AS-MQ) for uncomplicated vivax malaria was conducted in 3 district hospitals in Sabah, Malaysia. Primaquine was administered on day 28. The primary outcome was the cumulative risk of treatment failure by day 28 by Kaplan-Meier analysis.

    RESULTS: From 2012 to 2014, 103 adults and children were enrolled. Treatment failure by day 28 was 61.1% (95% confidence interval [CI], 46.8-75.6) after CQ and 0% (95% CI, 0-.08) following AS-MQ (P < .001), of which 8.2% (95% CI, 2.5-9.6) were early treatment failures. All patients with treatment failure had therapeutic plasma CQ concentrations at day 7. Compared with CQ, AS-MQ was associated with faster parasite clearance (normalized clearance slope, 0.311 vs 0.127; P < .001) and fever clearance (mean, 19.0 vs 37.7 hours; P =001) and with lower risk of anemia at day 28 (odds ratio = 3.7; 95% CI, 1.5-9.3; P =005). Gametocytes were present at day 28 in 23.8% (10/42) of patients following CQ vs none with AS-MQ (P < .001). AS-MQ resulted in lower bed occupancy: 4037 vs 6510 days/1000 patients (incidence rate ratio 0.62; 95% CI, .60-.65; P < .001). One patient developed severe anemia not regarded as related to their AS-MQ treatment.

    CONCLUSIONS: High-grade CQ-resistant P. vivax is prevalent in eastern Malaysia. AS-MQ is an efficacious ACT for all malaria species. Wider CQ-efficacy surveillance is needed in vivax-endemic regions with earlier replacement with ACT when treatment failure is detected.Clinical Trials Registration NCT01708876.

    MeSH terms: Adolescent; Adult; Aged; Antimalarials/therapeutic use*; Child; Child, Preschool; Chloroquine/pharmacology*; Drug Resistance*; Female; Humans; Infant; Male; Middle Aged; Mefloquine/therapeutic use*; Treatment Outcome; Artemisinins/therapeutic use*; Young Adult
  4. Love RR, Hossain SM, Hussain MM, Mostafa MG, Laudico AV, Siguan SS, et al.
    Eur J Cancer, 2016 06;60:107-16.
    PMID: 27107325 DOI: 10.1016/j.ejca.2016.03.011
    PURPOSE: In premenopausal women with metastatic hormone receptor-positive breast cancer, hormonal therapy is the first-line therapy. Gonadotropin-releasing hormone analogue + tamoxifen therapies have been found to be more effective. The pattern of recurrence risk over time after primary surgery suggests that peri-operative factors impact recurrence. Secondary analyses of an adjuvant trial suggested that the luteal phase timing of surgical oophorectomy in the menstrual cycle simultaneous with primary breast surgery favourably influenced long-term outcomes.

    METHODS: Two hundred forty-nine premenopausal women with incurable or metastatic hormone receptor-positive breast cancer entered a trial in which they were randomised to historical mid-luteal or mid-follicular phase surgical oophorectomy followed by oral tamoxifen treatment. Kaplan-Meier methods, the log-rank test, and multivariable Cox regression models were used to assess overall and progression-free survival (PFS) in the two randomised groups and by hormone-confirmed menstrual cycle phase.

    RESULTS: Overall survival (OS) and PFS were not demonstrated to be different in the two randomised groups. In a secondary analysis, OS appeared worse in luteal phase surgery patients with progesterone levels <2 ng/ml (anovulatory patients; adjusted hazard ratio 1.46, 95% confidence interval [CI]: 0.89-2.41, p = 0.14) compared with those in luteal phase with progesterone level of 2 ng/ml or higher. Median OS was 2 years (95% CI: 1.7-2.3) and OS at 4 years was 26%.

    CONCLUSIONS: The history-based timing of surgical oophorectomy in the menstrual cycle did not influence outcomes in this trial of metastatic patients. ClinicalTrials.gov number NCT00293540.

    Study site: Bangladesh, The Philippines, China, Nigeria,
    Indonesia, Malaysia, Taiwan, Morocco, and Vietnam
    MeSH terms: Adult; Breast Neoplasms/physiopathology; Breast Neoplasms/therapy*; Combined Modality Therapy/methods; Female; Follicular Phase/physiology; Humans; Luteal Phase/physiology; Ovariectomy/methods*; Tamoxifen/therapeutic use*; Treatment Outcome; Premenopause/physiology; Antineoplastic Agents, Hormonal/therapeutic use*
  5. Acharya UR, Mookiah MR, Koh JE, Tan JH, Noronha K, Bhandary SV, et al.
    Comput Biol Med, 2016 06 01;73:131-40.
    PMID: 27107676 DOI: 10.1016/j.compbiomed.2016.04.009
    Age-related Macular Degeneration (AMD) affects the central vision of aged people. It can be diagnosed due to the presence of drusen, Geographic Atrophy (GA) and Choroidal Neovascularization (CNV) in the fundus images. It is labor intensive and time-consuming for the ophthalmologists to screen these images. An automated digital fundus photography based screening system can overcome these drawbacks. Such a safe, non-contact and cost-effective platform can be used as a screening system for dry AMD. In this paper, we are proposing a novel algorithm using Radon Transform (RT), Discrete Wavelet Transform (DWT) coupled with Locality Sensitive Discriminant Analysis (LSDA) for automated diagnosis of AMD. First the image is subjected to RT followed by DWT. The extracted features are subjected to dimension reduction using LSDA and ranked using t-test. The performance of various supervised classifiers namely Decision Tree (DT), Support Vector Machine (SVM), Probabilistic Neural Network (PNN) and k-Nearest Neighbor (k-NN) are compared to automatically discriminate to normal and AMD classes using ranked LSDA components. The proposed approach is evaluated using private and public datasets such as ARIA and STARE. The highest classification accuracy of 99.49%, 96.89% and 100% are reported for private, ARIA and STARE datasets. Also, AMD index is devised using two LSDA components to distinguish two classes accurately. Hence, this proposed system can be extended for mass AMD screening.
    MeSH terms: Algorithms; Decision Trees; Diagnostic Techniques, Ophthalmological; Fundus Oculi; Macular Degeneration; Photography; Radon; Retina; Discriminant Analysis; Choroidal Neovascularization; Geographic Atrophy; Wavelet Analysis; Support Vector Machine; Ophthalmologists
  6. Selvaratnam C, Thevarajoo S, Ee R, Chan KG, Bennett JP, Goh KM, et al.
    Mar Genomics, 2016 Apr 20.
    PMID: 27107724 DOI: 10.1016/j.margen.2016.04.004
    Roseivirga sp. strain D-25 is an aerobic marine bacterium isolated from seawater collected from Desaru beach, Malaysia. To date, the genus Roseivirga consists of only four species with no genome sequence reported. Here, we present the genome sequence of Roseivirga sp. strain D-25 (=KCTC 42709=DSM 101709), with a genome size of approximately 4.08Mbp and G+C content of 39.18%. Genome sequence analysis of strain D-25 revealed the presence of genes related to petroleum hydrocarbon degradation, 2,4,6-trinitrotoluene detoxification, heavy metals bioremediation and production of carotenoids, which shed light on the potential application of this strain.
  7. Hussein Z, Taher SW, Gilcharan Singh HK, Chee Siew Swee W
    Ann Glob Health, 2016 4 25;81(6):851-62.
    PMID: 27108152 DOI: 10.1016/j.aogh.2015.12.016
    BACKGROUND: Diabetes is a major public health concern in Malaysia, and the prevalence of type 2 diabetes (T2D) has escalated to 20.8% in adults above the age of 30, affecting 2.8 million individuals. The burden of managing diabetes falls on primary and tertiary health care providers operating in various settings.

    OBJECTIVES: This review focuses on the current status of diabetes in Malaysia, including epidemiology, complications, lifestyle, and pharmacologic treatments, as well as the use of technologies in its management and the adoption of the World Health Organization chronic care model in primary care clinics.

    METHODS: A narrative review based on local available health care data, publications, and observations from clinic experience.

    FINDINGS: The prevalence of diabetes varies among the major ethnic groups in Malaysia, with Asian Indians having the highest prevalence of T2D, followed by Malays and Chinese. The increase prevalence of overweight and obesity has accompanied the rise in T2D. Multidisciplinary care is available in tertiary and primary care settings with integration of pharmacotherapy, diet, and lifestyle changes. Poor dietary adherence, high consumption of carbohydrates, and sedentary lifestyle are prevalent in patients with T2D. The latest medication options are available with increasing use of intensive insulin regimens, insulin pumps, and continuous glucose monitoring systems for managing glycemic control. A stepwise approach is proposed to expand the chronic care model into an Innovative Care for Chronic Conditions framework to facilitate implementation and realize better outcomes in primary care settings.

    CONCLUSIONS: A comprehensive strategy and approach has been established by the Malaysian government to improve prevention, treatment, and control of diabetes as an urgent response to this growing chronic disease.

    MeSH terms: Blood Glucose/analysis; Diabetes Mellitus, Type 2/complications; Diabetes Mellitus, Type 2/ethnology; Diabetes Mellitus, Type 2/epidemiology*; Humans; Hypoglycemic Agents/administration & dosage; Hypoglycemic Agents/therapeutic use; Insulin/administration & dosage; Insulin/therapeutic use; Life Style; Malaysia/epidemiology; Obesity/complications; Obesity/epidemiology*; Obesity/therapy; Primary Health Care/methods*; Body Mass Index; Prevalence; Diabetes Complications/epidemiology*
  8. Sefat F, Youseffi M, Khaghani SA, Soon CF, Javid F
    Cytokine, 2016 07;83:118-126.
    PMID: 27108397 DOI: 10.1016/j.cyto.2016.04.008
    Articular cartilage is an avascular and flexible connective tissue found in joints. It produces a cushioning effect at the joints and provides low friction to protect the ends of the bones from wear and tear/damage. It has poor repair capacity and any injury can result pain and loss of mobility. Transforming growth factor-beta (TGF-β), a cytokine superfamily, regulates cell function, including differentiation and proliferation. Although the function of the TGF-βs in various cell types has been investigated, their function in cartilage repair is as yet not fully understood. The effect of TGF-β3 in biological regulation of primary chondrocyte was investigated in this work. TGF-β3 provided fibroblastic morphology to chondrocytes and therefore overall reduction in cell proliferation was observed. The length of the cells supplemented with TGF-β3 were larger than the cells without TGF-β3 treatment. This was caused by the fibroblast like cells (dedifferentiated chondrocytes) which occupied larger areas compared to cells without TGF-β3 addition. The healing process of the model wound closure assay of chondrocyte multilayer was slowed down by TGF-β3, and this cytokine negatively affected the strength of chondrocyte adhesion to the cell culture surface.
    MeSH terms: Animals; Cell Adhesion/drug effects; Cells, Cultured; Fibroblasts/metabolism*; Wound Healing/drug effects*; Rats, Sprague-Dawley; Cell Culture Techniques; Chondrocytes/metabolism*; Chondrocytes/pathology; Cell Proliferation/drug effects*; Rats; Transforming Growth Factor beta3/pharmacology*; Cell Dedifferentiation/drug effects*
  9. Singh SK, Yahya N, Misiran K, Masdar A, Nor NM, Yee LC
    Braz J Anesthesiol, 2016 May-Jun;66(3):259-64.
    PMID: 27108822 DOI: 10.1016/j.bjane.2014.09.006
    BACKGROUND AND OBJECTIVES: Combined spinal-epidural (CSE) has become an increasingly popular alternative to traditional labour epidural due to its rapid onset and reliable analgesia provided. This was a prospective, convenient sampling study to determine the effects of CSE analgesia on labour outcome.

    METHODS: One hundred and ten healthy primigravida parturients with a singleton pregnancy of ≥37 weeks gestation and in the active phase of labour were studied. They were enrolled to the CSE (n=55) or Non-CSE (n=55) group based on whether they consented to CSE analgesia. Non-CSE parturients were offered other methods of labour analgesia. The duration of the first and second stage of labour, rate of instrumental vaginal delivery and emergency cesarean section, and Apgar scores were compared.

    RESULTS: The mean duration of the first and second stage of labour was not significantly different between both groups. Instrumental delivery rates between the groups were not significantly different (CSE group, 11% versus Non-CSE group, 16%). The slightly higher incidence of cesarean section in the CSE group (16% versus 15% in the Non-CSE group) was not statistically significant. Neonatal outcome in terms of Apgar score of less than 7 at 1 and 5min was similar in both groups.

    CONCLUSION: There were no significant differences in the duration of labour, rate of instrumental vaginal delivery and emergency cesarean section, and neonatal outcome in parturients who received compared to those who did not receive CSE for labour analgesia.
    MeSH terms: Adult; Anesthesia, Spinal/methods*; Drug Therapy, Combination; Female; Humans; Labor, Obstetric*; Pregnancy; Prospective Studies; Time Factors; Analgesia, Epidural/methods*; Analgesia, Obstetrical/methods*; Treatment Outcome; Delivery, Obstetric/statistics & numerical data*; Young Adult
  10. Yahaya NH, Teo R, Izaham A, Tang S, Mohamad Yusof A, Abdul Manap N
    Braz J Anesthesiol, 2016 May-Jun;66(3):283-8.
    PMID: 27108826 DOI: 10.1016/j.bjane.2014.10.008
    BACKGROUND AND OBJECTIVE: To evaluate the ability of anaesthetic trainee doctors compared to nursing anaesthetic assistants in identifying the cricoid cartilage, applying the appropriate cricoid pressure and producing an adequate laryngeal inlet view.

    METHODS: Eighty-five participants, 42 anaesthetic trainee doctors and 43 nursing anaesthetic assistants, were asked to complete a set of questionnaires which included the correct amount of force to be applied to the cricoid cartilage. They were then asked to identify the cricoid cartilage and apply the cricoid pressure on an upper airway manikin placed on a weighing scale, and the pressure was recorded. Subsequently they applied cricoid pressure on actual anaesthetized patients following rapid sequence induction. Details regarding the cricoid pressure application and the Cormack-Lehane classification of the laryngeal view were recorded.

    RESULTS: The anaesthetic trainee doctors were significantly better than the nursing anaesthetic assistants in identifying the cricoid cartilage (95.2% vs. 55.8%, p=0.001). However, both groups were equally poor in the knowledge about the amount of cricoid pressure force required (11.9% vs. 9.3% respectively) and in the correct application of cricoid pressure (16.7% vs. 20.9% respectively). The three-finger technique was performed by 85.7% of the anaesthetic trainee doctors and 65.1% of the nursing anaesthetic assistants (p=0.03). There were no significant differences in the Cormack-Lehane view between both groups.

    CONCLUSION: The anaesthetic trainee doctors were better than the nursing anaesthetic assistants in cricoid cartilage identification but both groups were equally poor in their knowledge and application of cricoid pressure.
    MeSH terms: Adult; Anesthesiology/education*; Clinical Competence/statistics & numerical data*; Cricoid Cartilage*; Female; Humans; Male; Manikins; Nurse Anesthetists/statistics & numerical data*; Physician Assistants/statistics & numerical data; Pressure; Prospective Studies; Surveys and Questionnaires; Single-Blind Method; Anesthesiologists/statistics & numerical data*
  11. Osuri AM, Ratnam J, Varma V, Alvarez-Loayza P, Hurtado Astaiza J, Bradford M, et al.
    Nat Commun, 2016 04 25;7:11351.
    PMID: 27108957 DOI: 10.1038/ncomms11351
    Defaunation is causing declines of large-seeded animal-dispersed trees in tropical forests worldwide, but whether and how these declines will affect carbon storage across this biome is unclear. Here we show, using a pan-tropical data set, that simulated declines of large-seeded animal-dispersed trees have contrasting effects on aboveground carbon stocks across Earth's tropical forests. In our simulations, African, American and South Asian forests, which have high proportions of animal-dispersed species, consistently show carbon losses (2-12%), but Southeast Asian and Australian forests, where there are more abiotically dispersed species, show little to no carbon losses or marginal gains (±1%). These patterns result primarily from changes in wood volume, and are underlain by consistent relationships in our empirical data (∼2,100 species), wherein, large-seeded animal-dispersed species are larger as adults than small-seeded animal-dispersed species, but are smaller than abiotically dispersed species. Thus, floristic differences and distinct dispersal mode-seed size-adult size combinations can drive contrasting regional responses to defaunation.
    MeSH terms: Animals; Carbon/analysis*; Conservation of Natural Resources; Trees/growth & development; Trees/chemistry; Tropical Climate; Ecosystem; Seed Dispersal; Animal Distribution*; Forests
  12. Chaurasia MK, Ravichandran G, Nizam F, Arasu MV, Al-Dhabi NA, Arshad A, et al.
    Fish Shellfish Immunol, 2016 Jul;54:353-63.
    PMID: 27109581 DOI: 10.1016/j.fsi.2016.04.031
    This study reports the comprehensive comparative information of two different detoxification enzymes such as glutathione S-transferases (GSTs) delta and kappa from freshwater giant prawn Macrobrachium rosenbergii (designated as MrGSTD and MrGSTK) by investigating their in-silico characters and mRNA modulation against various biotic and abiotic oxidative stressors. The physico-chemical properties of these cDNA and their polypeptide structure were analyzed using various bioinformatics program. The analysis indicated the variation in size of the polypeptides, presence or absence of domains and motifs and structure. Homology and phylogenetic analysis revealed that MrGSTD shared maximum identity (83%) with crustaceans GST delta, whereas MrGSTK fell in arthropods GST kappa. It is interesting to note that MrGSTD and MrGSTK shared only 21% identity; it indicated their structural difference. Structural analysis indicated that MrGSTD to be canonical dimer like shape and MrGSTK appeared to be butterfly dimer like shape, in spite of four β-sheets being conserved in both GSTs. Tissue specific gene expression analysis showed that both MrGSTD and MrGSTK are highly expressed in immune organs such as haemocyte and hepatopancreas, respectively. To understand the role of mRNA modulation of MrGSTD and MrGSTK, the prawns were inducted with oxidative stressors such as bacteria (Vibrio harveyi), virus [white spot syndrome virus (WSSV)] and heavy metal, cadmium (Cd). The analysis revealed an interesting fact that both MrGSTD and MrGSTK showed higher (P 
    MeSH terms: Amino Acid Sequence; Animals; Base Sequence; Computer Simulation; Glutathione Transferase/genetics*; Glutathione Transferase/metabolism; Glutathione Transferase/chemistry; Inactivation, Metabolic; Phylogeny; RNA, Messenger/genetics; RNA, Messenger/metabolism; Sequence Alignment/veterinary; DNA, Complementary/genetics; DNA, Complementary/metabolism; Oxidative Stress/physiology*; Virus Physiological Phenomena; Bacterial Physiological Phenomena; Metals, Heavy/toxicity; Palaemonidae/drug effects; Palaemonidae/genetics*; Palaemonidae/immunology*; Palaemonidae/microbiology; Arthropod Proteins/genetics*; Arthropod Proteins/metabolism; Arthropod Proteins/chemistry
  13. Wang XY, Lim-Jurado M, Prepageran N, Tantilipikorn P, Wang de Y
    Ther Clin Risk Manag, 2016;12:585-97.
    PMID: 27110120 DOI: 10.2147/TCRM.S105189
    Allergic rhinitis and urticaria are common allergic diseases that may have a major negative impact on patients' quality of life. Bilastine, a novel new-generation antihistamine that is highly selective for the H1 histamine receptor, has a rapid onset and prolonged duration of action. This agent does not interact with the cytochrome P450 system and does not undergo significant metabolism in humans, suggesting that it has very low potential for drug-drug interactions, and does not require dose adjustment in renal impairment. As bilastine is not metabolized and is excreted largely unchanged, hepatic impairment is not expected to increase systemic exposure above the drug's safety margin. Bilastine has demonstrated similar efficacy to cetirizine and desloratadine in patients with seasonal allergic rhinitis and, in a Vienna Chamber study, a potentially longer duration of action than fexofenadine in patients with asymptomatic seasonal allergic rhinitis. It has also shown significant efficacy (similar to that of cetirizine) and safety in the long-term treatment of perennial allergic rhinitis. Bilastine showed similar efficacy to levocetirizine in patients with chronic spontaneous urticaria and can be safely used at doses of up to fourfold higher than standard dosage (80 mg once daily). The fourfold higher than standard dose is specified as an acceptable second-line treatment option for urticaria in international guidelines. Bilastine is generally well tolerated, both at standard and at supratherapeutic doses, appears to have less sedative potential than other second-generation antihistamines, and has no cardiotoxicity. Based on its pharmacokinetic properties, efficacy, and tolerability profile, bilastine will be valuable in the management of allergic rhinitis and urticaria.
    MeSH terms: Benzimidazoles; Cytochrome P-450 Enzyme System; Drug Interactions; Rhinitis, Allergic, Seasonal; Histamine H1 Antagonists; Humans; Hypnotics and Sedatives; Piperidines; Quality of Life; Receptors, Histamine; Urticaria; Terfenadine; Cetirizine; Loratadine; Histamine H1 Antagonists, Non-Sedating
  14. Salihah N, Mazlan N, Lua PL
    J Multidiscip Healthc, 2016;9:145-51.
    PMID: 27110121 DOI: 10.2147/JMDH.S97695
    This study aimed to explore the subjective experience of nausea and vomiting during chemotherapy treatment among breast cancer patients and the impacts on their daily lives.
  15. Abdullahi A, Hassan A, Kadarman N, Saleh A, Baraya YS, Lua PL
    Int J Gen Med, 2016;9:79-87.
    PMID: 27110137 DOI: 10.2147/IJGM.S98436
    Foodborne diseases are common in the developing countries due to the predominant poor food handling and sanitation practices, particularly as a result of inadequate food safety laws, weak regulatory structures, and inadequate funding as well as a lack of appropriate education for food-handlers. The most frequently involved foods in disease outbreaks are of animal origin. However, in spite of the adequate legislation and laws governing the abattoir operation in Malaysia, compliance with food safety requirements during meat processing and waste disposal is inadequate. Therefore, the present study was designed to assess the food safety knowledge, attitude, and practice toward compliance with abattoir laws among the workers in Terengganu, Malaysia.
    MeSH terms: Abattoirs; Animals; Developing Countries; Disease Outbreaks; Food Handling; Foodborne Diseases; Humans; Legislation, Food; Malaysia; Meat; Sanitation; Food Safety
  16. Likhitrakarn N, Golovatch SI, Panha S
    Zookeys, 2016.
    PMID: 27110157 DOI: 10.3897/zookeys.571.7566
    The genus Antheromorpha is redefined and shown to comprise 11 valid species: Antheromorpha miranda (Pocock, 1895), Antheromorpha bistriata (Pocock, 1895), Antheromorpha comotti (Pocock, 1895), Antheromorpha festiva (Brölemann, 1896), Antheromorpha harpaga (Attems, 1937), Antheromorpha mediovirgata (Carl, 1941), Antheromorpha minlana (Pocock, 1895), Antheromorpha pardalis (Pocock, 1895), Antheromorpha paviei (Brölemann, 1896), comb. n., Antheromorpha rosea Golovatch, 2013 and Antheromorpha uncinata (Attems, 1931). Three new synonymies are proposed: Antheromorpha bivittata (Pocock, 1895) and Antheromorpha melanopleuris (Pocock, 1895) are synonymized under Antheromorpha miranda (Pocock, 1895), and Antheromorpha orophila (Carl, 1941) under Antheromorpha comotti (Pocock, 1895). Detailed descriptions and illustrations of fresh material from Thailand and Malaysia are given, especially regarding colour patterns which appear to be crucial for accurate species identifications. Two Antheromorpha species proposed by Attems are redescribed, based on type material. The genus is rediagnosed and a key and a distribution map are also provided. At least in Thailand, adult Antheromorpha rosea have been found to occur every year only for one or two weeks in September or October, disappearing thereafter.
    MeSH terms: Animals; Arthropods; Color; Excipients; Malaysia; Thailand; Ciliophora; Animal Distribution
  17. Ng CG, Lai KT, Tan SB, Sulaiman AH, Zainal NZ
    J Palliat Med, 2016 09;19(9):917-24.
    PMID: 27110900 DOI: 10.1089/jpm.2016.0046
    BACKGROUND: Palliative cancer patients suffer from high levels of distress. There are physiological changes in relation to the level of perceived distress.

    OBJECTIVE: To study the efficacy of 5 minutes of mindful breathing (MB) for rapid reduction of distress in a palliative setting. Its effect to the physiological changes of the palliative cancer patients was also examined.

    METHODS: This is a randomized controlled trial. Sixty palliative cancer patients were recruited. They were randomly assigned to either 5 minutes of MB or normal listening arms. The changes of perceived distress, blood pressure, pulse rate, breathing rate, galvanic skin response, and skin surface temperature of the patients were measured at baseline, after intervention, and 10 minutes post-intervention.

    RESULTS: There was significant reduction of perceived distress, blood pressure, pulse rate, breathing rate, and galvanic skin response; also, significant increment of skin surface temperature in the 5-minute MB group. The changes in the 5-minute breathing group were significantly higher than the normal listening group.

    CONCLUSION: Five-minute MB is a quick, easy to administer, and effective therapy for rapid reduction of distress in palliative setting. There is a need for future study to establish the long-term efficacy of the therapy.

    MeSH terms: Blood Pressure; Heart Rate; Humans; Music Therapy; Neoplasms; Palliative Care*
  18. Chuah CJ, Mukhaidin N, Choy SH, Smith GJD, Mendenhall IH, Lim YAL, et al.
    Sci Total Environ, 2016 08 15;562:701-713.
    PMID: 27110981 DOI: 10.1016/j.scitotenv.2016.03.247
    A catchment-scale investigation of the prevalence of Cryptosporidium and Giardia in the Kuang River Basin was carried out during the dry and rainy seasons. Water samples were collected from the Kuang River and its tributaries as well as a major irrigation canal at the study site. We also investigated the prevalence of gastrointestinal parasitic infection among dairy and beef cattle hosts. Cryptosporidium and/or Giardia were detected in all the rivers considered for this study, reflecting their ubiquity within the Kuang River Basin. The high prevalence of Cryptosporidium/Giardia in the upper Kuang River and Lai River is of a particular concern as both drain into the Mae Kuang Reservoir, a vital source of drinking-water to many local towns and villages at the research area. We did not, however, detected neither Cryptosporidium nor Giardia were in the irrigation canal. The frequency of Cryptosporidium/Giardia detection nearly doubled during the rainy season compared to the dry season, highlighting the importance of water as an agent of transport. In addition to the overland transport of these protozoa from their land sources (e.g. cattle manure, cess pits), Cryptosporidium/Giardia may also be re-suspended from the streambeds (a potentially important repository) into the water column of rivers during storm events. Faecal samples from dairy and beef cattle showed high infection rates from various intestinal parasites - 97% and 94%, respectively. However, Cryptosporidium and Giardia were only detected in beef cattle. The difference in management style between beef (freeranging) and dairy cattle (confined) may account for this disparity. Finally, phylogenetic analyses revealed that the Cryptosporidium/Giardia-positive samples contained C. ryanae (non-zoonotic) as well as Giardia intestinalis assemblages B (zoonotic) and E (non-zoonotic). With only basic water treatment facilities afforded to them, the communities of the rural area relying on these water supplies are highly at risk to Cryptosporidium/Giardia infections.
    MeSH terms: Cryptosporidium/growth & development*; Environmental Monitoring*; Giardia/growth & development*; Thailand; Water Supply/statistics & numerical data*; Rivers/parasitology*; Water Resources
  19. Hossain M, Mani KK, Mohd Sidik S, Kadir Shahar H
    Acta Paediatr, 2016 Aug;105(8):e373-8.
    PMID: 27111395 DOI: 10.1111/apa.13447
    AIM: There are an estimated 372 000 worldwide deaths by drowning every year, and it has been described as a secret epidemic in Bangladesh. The aim of this study was to develop an intervention package to prevent children under the age of five from drowning in rural Bangladesh.

    METHODS: This was a qualitative study using focus group discussions in three villages in rural Bangladesh. The 45 participants were mothers and fathers with children under five, the parents of children who had drowned and community leaders.

    RESULTS: The majority of the participants (71%) were male. The focus groups revealed that most drowning's occurred between 11am and 2pm and that risk factors included the following: children not being able to swim, ditches that were not filled in, lack of medical facilities, parents who were not aware of childhood drowning and lack of information through the media about how to prevent of childhood drowning. Suggestions included using a mobile-based short messaging service or voice calls to parents, especially mothers, could increase awareness and reduce the risk of childhood drowning.

    CONCLUSION: A safety education programme could be effective in increasing knowledge and changing attitudes, which could prevent drowning among children in Bangladesh.

    MeSH terms: Adolescent; Adult; Bangladesh; Child, Preschool; Drowning/prevention & control*; Female; Humans; Infant; Male; Middle Aged; Parents; Rural Population; Focus Groups*; Young Adult
  20. Steuernagel B, Periyannan SK, Hernández-Pinzón I, Witek K, Rouse MN, Yu G, et al.
    Nat Biotechnol, 2016 Jun;34(6):652-5.
    PMID: 27111722 DOI: 10.1038/nbt.3543
    Wild relatives of domesticated crop species harbor multiple, diverse, disease resistance (R) genes that could be used to engineer sustainable disease control. However, breeding R genes into crop lines often requires long breeding timelines of 5-15 years to break linkage between R genes and deleterious alleles (linkage drag). Further, when R genes are bred one at a time into crop lines, the protection that they confer is often overcome within a few seasons by pathogen evolution. If several cloned R genes were available, it would be possible to pyramid R genes in a crop, which might provide more durable resistance. We describe a three-step method (MutRenSeq)-that combines chemical mutagenesis with exome capture and sequencing for rapid R gene cloning. We applied MutRenSeq to clone stem rust resistance genes Sr22 and Sr45 from hexaploid bread wheat. MutRenSeq can be applied to other commercially relevant crops and their relatives, including, for example, pea, bean, barley, oat, rye, rice and maize.
    MeSH terms: Cloning, Molecular/methods*; Plant Diseases/genetics*; Plant Diseases/prevention & control; Plants/genetics*; Mutagenesis, Site-Directed/methods*; Genes, Plant/genetics*; Sequence Analysis, DNA/methods; Genetic Enhancement/methods; Plants, Genetically Modified/genetics; Disease Resistance/genetics*
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