Browse publications by year: 2016

  1. Kurz AS, Drescher CF, Chin EG, Johnson LR
    Psych J, 2016 Jun;5(2):92-100.
    PMID: 27168227 DOI: 10.1002/pchj.124
    Malaysia is a Southeast Asian country in which multiple languages are prominently spoken, including English and Mandarin Chinese. As psychological science continues to develop within Malaysia, there is a need for psychometrically sound instruments that measure psychological phenomena in multiple languages. For example, assessment tools for measuring social desirability could be a useful addition in psychological assessments and research studies in a Malaysian context. This study examined the psychometric performance of the English and Mandarin Chinese versions of the Marlowe-Crowne Social Desirability Scale when used in Malaysia. Two hundred and eighty-three students (64% female; 83% Chinese, 9% Indian) from two college campuses completed the Marlowe-Crowne Social Desirability Scale in their language of choice (i.e., English or Mandarin Chinese). Proposed factor structures were compared with confirmatory factor analysis, and multiple indicators-multiple causes models were used to examine measurement invariance across language and sex. Factor analyses supported a two-factor structure (i.e., Attribution and Denial) for the measure. Invariance tests revealed the scale was invariant by sex, indicating that social desirability can be interpreted similarly across sex. The scale was partially invariant by language version, with some non-invariance observed within the Denial factor. Non-invariance may be related to differences in the English and Mandarin Chinese languages, as well as cultural differences. Directions for further research include examining the measurement of social desirability in other contexts where both English and Mandarin Chinese are spoken (i.e., China) and further examining the causes of non-invariance on specific items.
  2. Tay TK, Chan HZ, Ahmad TS, Teh KK, Low TH, Wahab NA
    J Occup Med Toxicol, 2016;11:23.
    PMID: 27168760 DOI: 10.1186/s12995-016-0112-y
    BACKGROUND: Marine stings and envenomation are fairly common in Malaysia. Possible contact to various marine life occurs during diving, fishing and food handling. Even though majority of fish stings are benign, there are several venomous species such as puffer fish, scorpion fish, lionfish, stingray and stonefish that require urgent medical treatment. Stonefish is one of the most venomous fish in the world with potential fatal local and systemic toxicity effects to human.

    CASE PRESENTATION: We reported a case of stonefish sting complicated with impending compartment syndrome.

    CONCLUSIONS: Medical staff should be alert about the possibility of this potential emergency in standard management of stonefish stings.

    MeSH terms: Animals; Bites and Stings; Compartment Syndromes; Diving; Emergencies; Fish Venoms; Fishes, Poisonous; Food Handling; Humans; Malaysia; Medical Staff; Scorpions; Skates (Fish); Venoms; Tetraodontiformes
  3. Masum AK, Azad MA, Hoque KE, Beh LS, Wanke P, Arslan Ö
    PeerJ, 2016;4:e1896.
    PMID: 27168960 DOI: 10.7717/peerj.1896
    The aim of this study was to identify the facets influencing job satisfaction and intention to quit of nurses employed in Turkey. Using a non-probability sampling technique, 417 nurses from six large private hospitals were surveyed from March 2014 to June 2014. The nurses' demographic data, their job-related satisfaction and turnover intentions were recorded through a self-administered questionnaire. In this study, descriptive and bivariate analyses were used to explore data, and multivariate analysis was performed using logistic regression. Nurses' job satisfaction was found at a moderate level with 61% of the nurses intended to quit. Nevertheless, nurses reported a high satisfaction level with work environment, supervisor support, and co-workers among the selected nine facets of job satisfaction. They also reported a low satisfaction level with contingent reward, fringe benefits, and pay. The impact of demographic characteristics on job satisfaction and intention to quit was also examined. The study revealed a negative relationship between job satisfaction and intention to quit the existing employment. Moreover, satisfaction with supervisor support was the only facet that significantly explained turnover intent when controlling for gender, age, marital status, education, and experience. The implications for nurse management were also described for increasing nurses' job satisfaction and retention. This study is beneficial for hospital management to ensure proper nursing care that would lead to a better quality healthcare service.
    MeSH terms: Demography; Humans; Job Satisfaction; Personal Satisfaction; Personnel Turnover; Surveys and Questionnaires; Reward; Salaries and Fringe Benefits; Turkey; Multivariate Analysis; Logistic Models; Hospitals, Private; Workplace; Marital Status; Intention
  4. Dieye Y, Mbengue B, Dagamajalu S, Fall MM, Loke MF, Nguer CM, et al.
    PeerJ, 2016;4:e1965.
    PMID: 27168977 DOI: 10.7717/peerj.1965
    Background. With 214 million cases and 438,000 deaths in 2015, malaria remains one of the deadliest infectious diseases in tropical countries. Several species of the protozoan Plasmodium cause malaria. However, almost all the fatalities are due to Plasmodium falciparum, a species responsible for the severest cases including cerebral malaria. Immune response to Plasmodium falciparum infection is mediated by the production of pro-inflammatory cytokines, chemokines and growth factors whose actions are crucial for the control of the parasites. Following this response, the induction of anti-inflammatory immune mediators downregulates the inflammation thus preventing its adverse effects such as damages to various organs and death. Methods. We performed a retrospective, nonprobability sampling study using clinical data and sera samples from patients, mainly adults, suffering of non-cerebral or cerebral malaria in Dakar, Sénégal. Healthy individuals residing in the same area were included as controls. We measured the serum levels of 29 biomarkers including growth factors, chemokines, inflammatory and anti-inflammatory cytokines. Results. We found an induction of both pro- and anti-inflammatory immune mediators during malaria. The levels of pro-inflammatory biomarkers were higher in the cerebral malaria than in the non-cerebral malaria patients. In contrast, the concentrations of anti-inflammatory cytokines were comparable in these two groups or lower in CM patients. Additionally, four pro-inflammatory biomarkers were significantly increased in the deceased of cerebral malaria compared to the survivors. Regarding organ damage, kidney failure was significantly associated with death in adults suffering of cerebral malaria. Conclusions. Our results suggest that a poorly controlled inflammatory response determines a bad outcome in African adults suffering of cerebral malaria.
    MeSH terms: Adult; Animals; Anti-Inflammatory Agents; Humans; Inflammation; Parasites; Plasmodium; Plasmodium falciparum; Retrospective Studies; Sampling Studies; Senegal; Biomarkers; Cytokines; Malaria, Falciparum; Malaria, Cerebral; Survivors; Chemokines; Renal Insufficiency
  5. Kashou A, Durairajanayagam D, Agarwal A
    World J Mens Health, 2016 Apr;34(1):9-19.
    PMID: 27169124 DOI: 10.5534/wjmh.2016.34.1.9
    Since its inception in 2008, the American Center for Reproductive Medicine's summer internship program in reproductive research and writing has trained 114 students from 23 states within the United States and 10 countries worldwide. Its fundamental goal is to inspire pre-medical and medical students to embrace a career as a physician-scientist. During this intensive course, established scientists and clinicians train interns in the essential principles and fundamental concepts of bench research and scientific writing. Over the first six years (2008~2013), interns have collectively published 98 research articles and performed 12 bench research projects on current and emerging topics in reproductive medicine. Interns have also developed and honed valuable soft skills including time management, communication and presentation skills, as well as life values, which all enhance personal and professional satisfaction. Program graduates are able to recognize the value of medical research and its potential to impact patient care and gain insight into their own career pathway. Between 2011 and 2014, the internship program was thrice awarded a Scholarship in Teaching Award by Case Western Reserve School of Medicine for its innovative teaching approach and positive impact on medical education and student careers. This report highlights the demographics, logistics, implementation, feedback, and results of the first six years of the American Center for Reproductive Medicine's summer internship program at Cleveland Clinic (Cleveland, OH, USA). This may be helpful to other research and academic institutions considering implementing a similar program. In addition, it creates awareness among potential physician-scientists of what the world of research has to offer in both scientific writing and bench research. Finally, it may stimulate further discussion regarding narrowing the gap between physicians and scientists and refinement of the current program.
    MeSH terms: Awards and Prizes; Demography; Education, Medical; Feedback; Fellowships and Scholarships; Patient Care; Goals; Humans; Internship and Residency; Personal Satisfaction; Students, Medical; United States; Writing; Time Management; Reproductive Medicine; Biomedical Research
  6. Kwan TK, Shipton F, Azman NS, Hossan S, Jin KT, Wiart C
    Nat Prod Commun, 2016 Mar;11(3):389-92.
    PMID: 27169188
    Artabotrys crassifolius Hook. f. & Thomson is a medicinal plant used in Malaysia. The cytotoxic effects of the hexane, chloroform and ethanol extracts of the leaves and bark were examined in vitro against MCF-7, MDA-468 and HCT-116 cells. The chloroform extract of the bark inhibited the growth of all cell lines with GI₅₀ values ranging from 4.2 µg/mL to 9.4 µg/mL. Silica gel column chromatography of this extract yielded artabotrine, liridine, atherospermidine and lysicamine. Artabotrine and lysicamine inhibited the growth of HCT-116 and MCF-7 cells with GI₅₀ values ranging from 3.3 µM to 3.9 µM. These alkaloids were not toxic to human embryonic kidney cells (HEK297) up to a concentration of 50 µg/mL.
    MeSH terms: Antineoplastic Agents, Phytogenic/pharmacology*; Antineoplastic Agents, Phytogenic/chemistry*; Humans; Models, Molecular; Molecular Structure; Plant Leaves/chemistry; Plant Bark/chemistry; Annonaceae/chemistry*; Cell Line, Tumor
  7. Besouw MT, Vande Walle JG, Ilias MI, Raes AM, Prytula AA, Claeys L, et al.
    Acta Clin Belg, 2016 Dec;71(6):455-457.
    PMID: 27169353
    A 12-year-old Congolese girl presented with acute renal failure, edema, hypertension, hemoptysis, hematuria, and proteinuria after a history of throat infection. Renal ultrasound showed kidneys of normal size, with increased echogenicity of the cortical parenchyma and decreased corticomedullary differentiation. Other additional investigations showed pancytopenia with decreased complement (low C3 and C4). Antinuclear antibodies were strongly positive, including anti-double stranded DNA. Renal biopsy confirmed severe grade IV lupus nephritis. She was treated with high-dose steroids, mycophenolate mofetil and hydroxychloroquine, in addition to hemodialysis. After one week of intensive treatment, diuresis recovered and dialysis could be stopped after six sessions. We describe an uncommon case of severe lupus nephritis, presenting with terminal renal failure. Since the rarity of this disease presentation, other more common diagnoses have to be considered. Once the diagnosis of lupus nephritis is established, a choice has to be made between the different induction treatment protocols. The patient's ethnic background and other supportive therapies, such as the need for dialysis, can help to make this choice.
    MeSH terms: Biopsy; Child; Female; Humans; Kidney Failure, Chronic/diagnosis; Kidney Failure, Chronic/etiology*; Lupus Nephritis/complications*; Lupus Nephritis/diagnosis; Ultrasonography; Disease Progression
  8. Tay BY, Yung SC, Teoh TY
    Int J Cosmet Sci, 2016 Dec;38(6):627-633.
    PMID: 27169828 DOI: 10.1111/ics.12342
    OBJECTIVE: Isopropyl p-toluenesulfonate (IPTS) is a potentially genotoxic by-product formed during the esterification of palm oil-based palmitic and palm kernel oil-based myristic acid with isopropanol to produce isopropyl palmitate or isopropyl myristate. There are no methods described for the analysis of IPTS in cosmetic products. In this work, we have established a simple, precise and accurate method to determine the presence and level of IPTS in various finished cosmetic products which contain palm-based esters in their formulations.

    METHODS: An Agilent 1200 series high-performance liquid chromatography (HPLC) unit using a diode-array detector (DAD) has been employed and optimized to detect IPTS in cosmetic products. For the separation, a reverse-phase Hypersil Gold C8 column (5 μm, 4.6 mm i.d. 250 mm) 5 mM tetrabutylammonium phosphate buffer 50 : 50, (v/v) solution in acetonitrile as mobile phase, in isocratic mode and a flow rate of 0.8 mL min(-1) were used. A second method using a gas chromatography/mass selective detector GC-MSD was also developed to confirm the IPTS identity in the cosmetic products.

    RESULTS: Recoveries of IPTS from cosmetic matrices such as a lotion, cleansing milk and a cream ranged from 94.0% to 101.1% with <5% relative standard deviation (%RSD) showing good accuracy and repeatability of the method. The six-point calibration curves (determined over the range 0.5-50 μg mL(-1) ) have a correlation coefficient of 0.9999 (based on HPLC peak area) and 0.9998 (based on HPLC peak height). The intra- and interday precisions (measured by the %RSD) of the method were <2% and <5%, respectively, indicating that the developed method is reliable, precise and reproducible. The detection and quantification limit of the method were found to be 0.5 μg mL(-1) and 1.6 μg mL(-1) , respectively. Analyses of 83 commercial cosmetics showed no presence of IPTS.

    CONCLUSIONS: The validation data indicated that this method was suitable for the quantitative analysis of IPTS in commercial cosmetics. This method is applicable for analyses of trace levels of IPTS in cosmetics and has the advantage of using only simple sample preparation steps.

    MeSH terms: Benzenesulfonates/chemistry*; Chromatography, High Pressure Liquid/methods*; Cosmetics*; Gas Chromatography-Mass Spectrometry/methods*; Reference Standards
  9. Kim M, Meurette G, Ragu R, Lehur PA
    Tech Coloproctol, 2016 Jun;20(6):395-399.
    PMID: 27170284 DOI: 10.1007/s10151-016-1473-z
    BACKGROUND: The aim of this study was to perform a survey on the surgical management of obstructed defecation (OD) across advocated selected coloproctological experts across Europe.

    METHODS: Surgeons from 42 centers of coloproctology in Europe were asked to complete a questionnaire, including seven questions about their past and present operative treatment strategy for patients with OD.

    RESULTS: The questionnaire was completed by 32 experts of pelvic floor surgery in 13 European countries. All but one indicated that they consider surgical treatment for OD. Seventy-four percent of these have been using transanal stapled rectal resection (STARR) and 96 % transabdominal rectopexy. While only 65 %, who have begun performing STARR are still using transanal resection, the technique is still being used by all surgeons performing abdominal procedures. Rectopexy only, STARR only, or both approaches are offered by 52, 3, and 45 % of surgeons, respectively.

    CONCLUSIONS: The use of STARR in the treatment of OD is decreasing among European opinion leaders in the field of pelvic floor surgery, while the application of transabdominal procedures continues.

    MeSH terms: Abdomen/surgery; Anal Canal/surgery; Colorectal Surgery/methods; Colorectal Surgery/statistics & numerical data*; Constipation/etiology; Constipation/surgery*; Defecation; Europe; Humans; Intestinal Obstruction/complications; Intestinal Obstruction/surgery*; Surveys and Questionnaires; Digestive System Surgical Procedures/methods; Digestive System Surgical Procedures/statistics & numerical data*; Pelvic Floor/surgery*; Surgical Stapling; Surgeons/statistics & numerical data
  10. Imran FH, Karim R, Maat NH
    J Med Case Rep, 2016;10(1):120.
    PMID: 27170287 DOI: 10.1186/s13256-016-0918-3
    Successful wound healing depends on various factors, including exudate control, prevention of microbial contaminants, and moisture balance. We report two cases of managing burn wounds with SMARTPORE Technology polyurethane foam dressing.
  11. Keith SA, Maynard JA, Edwards AJ, Guest JR, Bauman AG, van Hooidonk R, et al.
    Proc Biol Sci, 2016 05 11;283(1830).
    PMID: 27170709 DOI: 10.1098/rspb.2016.0011
    Coral spawning times have been linked to multiple environmental factors; however, to what extent these factors act as generalized cues across multiple species and large spatial scales is unknown. We used a unique dataset of coral spawning from 34 reefs in the Indian and Pacific Oceans to test if month of spawning and peak spawning month in assemblages of Acropora spp. can be predicted by sea surface temperature (SST), photosynthetically available radiation, wind speed, current speed, rainfall or sunset time. Contrary to the classic view that high mean SST initiates coral spawning, we found rapid increases in SST to be the best predictor in both cases (month of spawning: R(2) = 0.73, peak: R(2) = 0.62). Our findings suggest that a rapid increase in SST provides the dominant proximate cue for coral mass spawning over large geographical scales. We hypothesize that coral spawning is ultimately timed to ensure optimal fertilization success.
    MeSH terms: Animals; Pacific Ocean; Photosynthesis; Rain; Reproduction; Seasons; Sunlight; Temperature; Wind; Indian Ocean; Anthozoa/physiology*; Coral Reefs; Spatio-Temporal Analysis
  12. Shamsuddin AM, Chen YC, Wong AR, Le TP, Anderson RH, Corno AF
    Interact Cardiovasc Thorac Surg, 2016 Aug;23(2):231-4.
    PMID: 27170744 DOI: 10.1093/icvts/ivw129
    OBJECTIVES: Doubly committed ventricular septal defects (VSDs) account for up to almost one-third of isolated ventricular septal defects in Asian countries, compared with only 1/20th in western populations. In our surgical experience, this type of defect accounted for almost three-quarters of our practice. To date, patch closure has been considered the gold standard for surgical treatment of these lesions. Our objectives are to evaluate the indications and examine the outcomes of surgery for doubly committed VSDs.

    METHODS: Between October 2013, when our service of paediatric cardiac surgery was opened, and December 2014, 24 patients were referred for surgical closure of VSDs. Among them, 17 patients (71%), with the median age of 6 years, ranging from 2 to 9 years, and with a median body weight of 19 kg, ranging from 11 to 56 kg, underwent surgical repair for doubly committed defects. In terms of size, the defect was considered moderate in 4 and large in 13. Aortic valvular regurgitation (AoVR) was present in 11 patients (65%) preoperatively, with associated malformations found in 14 (82%), with 5 patients (29%) having two or more associated defects.

    RESULTS: After surgery, there was trivial residual shunting in 2 patients (12%). AoVR persisted in 6 (35%), reducing to trivial in 5 (29%) and mild in 1 (6%). Mean stays in the intensive care unit and hospital were 2.6 ± 1.2 days, ranging from 2 to 7 days, and 6.8 ± 0.8 days, ranging from 6 to 9 days, respectively. The mean follow-up was 14 ± 4 months, ranging from 6 to 20 months, with no early or late deaths and without clinical deterioration.

    CONCLUSIONS: The incidence of doubly committed lesions is high in our experience, frequently associated with AoVR and other associated malformation. Early detection is crucial to prevent further progression of the disease. Patch closure remains the gold standard in management, not least since it allows simultaneous repair of associated intracardiac defects.

    MeSH terms: Abnormalities, Multiple*; Adolescent; Aortic Valve Insufficiency/congenital; Aortic Valve Insufficiency/surgery*; Child; Child, Preschool; Female; Heart Septal Defects, Ventricular/surgery*; Cardiac Surgical Procedures/methods*; Humans; Male; Young Adult
  13. Frederick DA, Sandhu G, Morse PJ, Swami V
    Body Image, 2016 Jun;17:191-203.
    PMID: 27172812 DOI: 10.1016/j.bodyim.2016.04.001
    We examined the prevalence and correlates of satisfaction with appearance and weight. Participants (N=12,176) completed an online survey posted on the NBCNews.com and Today.com websites. Few men and women were very to extremely dissatisfied with their physical appearances (6%; 9%), but feeling very to extremely dissatisfied with weight was more common (15%; 20%). Only about one-fourth of men and women felt very to extremely satisfied with their appearances (28%; 26%) and weights (24%; 20%). Men and women with higher body masses reported higher appearance and weight dissatisfaction. Dissatisfied people had higher Neuroticism, more preoccupied and fearful attachment styles, and spent more hours watching television. In contrast, satisfied people had higher Openness, Conscientious, and Extraversion, were more secure in attachment style, and had higher self-esteem and life satisfaction. These findings highlight the high prevalence of body dissatisfaction and the factors linked to dissatisfaction among U.S. adults.
  14. Sukadarin EH, Deros BM, Ghani JA, Mohd Nawi NS, Ismail AR
    Int J Occup Saf Ergon, 2016 Sep;22(3):389-98.
    PMID: 27173135 DOI: 10.1080/10803548.2016.1156924
    INTRODUCTION: This review describes standardized ergonomics assessment based on pen-and-paper observational methods for assessing ergonomics risk factors.

    OBJECTIVE: The three main objectives are to analyze published pen-and-paper observational methods, to extract and understand the risk levels of each method and to identify their associated health effects.

    METHODOLOGY: The authors searched scientific databases and the Internet for materials from 1970 to 2013 using the following keywords: ergo, posture, method, observational, postural angle, health effects, pain and diseases. Postural assessments of upper arms, lower arms, wrists, neck, back and legs in six pen-and-paper-based observational methods are highlighted, extracted in groups and linked with associated adverse health effects.

    RESULTS: The literature reviewed showed strengths and limitations of published pen-and-paper-based observational methods in determining the work activities, risk levels and related postural angles to adverse health effects. This provided a better understanding of unsafe work postures and how to improve these postures.

    CONCLUSION: Many pen-and-paper-based observational methods have been developed. However, there are still many limitations of these methods. There is, therefore, a need to develop a new pen-and-paper-based observational method for assessing postural problems.

    MeSH terms: Humans; Human Engineering/methods*; Musculoskeletal Diseases/prevention & control; Occupational Diseases/prevention & control; Posture*; Risk Factors
  15. Etemad A, Vasudevan R, Aziz AF, Yusof AK, Khazaei S, Fawzi N, et al.
    Genet. Mol. Res., 2016 Apr 07;15(2).
    PMID: 27173202 DOI: 10.4238/gmr.15025845
    Type 2 diabetes mellitus (T2DM) is believed to be associated with excessive production of reactive oxygen species. Glutathione S-transferase (GST) polymorphisms result in decreased or absent enzyme activity and altered oxidative stress, and have been associated with cardiovascular disease (CVD). The present study assessed the effect of GST polymorphisms on the risk of developing T2DM in individuals of Malaysian Malay ethnicity. A total of 287 subjects, consisting of 87 T2DM and 64 CVD/T2DM patients, as well as 136 healthy gender- and age-matched controls were genotyped for selected polymorphisms to evaluate associations with T2DM susceptibility. Genomic DNA was extracted using commercially available kits, and GSTM1, GSTT1, and α-globin sequences were amplified by multiplex polymerase chain reaction. Biochemical parameters were measured with a Hitachi autoanalyzer. The Fisher exact test, the chi-square statistic, and means ± standard deviations were calculated using the SPSS software. Overall, we observed no significant differences regarding genotype and allele frequencies between each group (P = 0.224 and 0.199, respectively). However, in the combined analysis of genotypes and blood measurements, fasting plasma glucose, HbA1c, and triglyceride levels, followed by age, body mass index, waist-hip ratio, systolic blood pressure, and history of T2DM significantly differed according to GST polymorphism (P ˂ 0.05). Genetically induced absence of the GSTT1 enzyme is an independent and powerful predictor of premature vascular morbidity and death in individuals with T2DM, and might be triggered by cigarette smoking's oxidative effects. These polymorphisms could be screened in other ethnicities within Malaysia to determine further possible risk factors.
    MeSH terms: Alleles; Cardiovascular Diseases/complications; Cardiovascular Diseases/genetics*; Diabetes Mellitus, Type 2/complications; Diabetes Mellitus, Type 2/genetics*; Female; Genotype; Glutathione Transferase/genetics*; Humans; Malaysia; Male; Case-Control Studies; Polymorphism, Single Nucleotide*; alpha-Globins/genetics
  16. Lee TY, Lai MI, Ismail P, Ramachandran V, Tan JA, Teh LK, et al.
    Genet. Mol. Res., 2016 Apr 07;15(2).
    PMID: 27173219 DOI: 10.4238/gmr.15027400
    Hemoglobin (Hb) Adana [HBA2: c179G>A (or HBA1); p.Gly60Asp] is a non-deletional α-thalassemia variant found in Malaysia. An improvement in the molecular techniques in recent years has made identification of Hb Adana much easier. For this study, a total of 26 Hb Adana α-thalassemia intermedia and 10 Hb Adana trait blood samples were collected from patients. Common deletional and non-deletional α-thalassemia genotypes were determined using multiplex gap polymerase chain reaction (PCR) and multiplex ARMS PCR techniques. Identification of the Hb Adana location on the α-globin gene was carried out using genomic sequencing and the location of the mutation was confirmed via restriction fragment length polymorphism-PCR. Among the 36 samples, 24 (66.7%) had the -α(3.7)/α(Cd59)α mutation, while the -α(3.7)/α(Cd59)α mutation accounted for 2 samples (5.6%) and the remaining 10 (27.8%) samples were α/α(Cd59)α. All 36 samples were found to have the Hb Adana mutation on the α2-globin gene. The position of the α-globin gene mutation found in our cases was similar to that reported in Indonesia (16%) but not to that in Turkey (0.6%). Our results showed that the Hb Adana mutation was preferentially present in the α2-globin genes in Malays compared to the other ethnicities in Malaysia. Thus, the Malays might have similar ancestry based on the similarities in the Hb Adana position.
    MeSH terms: Hemoglobin A/genetics*; Hemoglobins, Abnormal/genetics*; Humans; Malaysia; Polymorphism, Restriction Fragment Length; alpha-Thalassemia/ethnology; alpha-Thalassemia/genetics*; Point Mutation
  17. Lim SJ, Wan Aida WM, Maskat MY, Latip J, Badri KH, Hassan O, et al.
    Food Chem, 2016 Oct 15;209:267-73.
    PMID: 27173562 DOI: 10.1016/j.foodchem.2016.04.058
    Fucoidan is a sulphated polysaccharide that consists mainly of fucose, normally found in brown seaweeds. In this study, fucoidan was extracted from Sargassum binderi (Fsar) from Malaysia and subsequently characterised. The chemical characteristics of Fsar were found to be different than those of commercial food grade fucoidan (Fysk) and those of previously studied fucoidans. NMR analysis proposed that the main structure of Fsar is →3)fuc-2-OSO3(-)(1→3)fuc(1→. The molecular weight (47.87kDa) and degree of sulphation (0.20) of Fsar were higher than those of Fysk, at 27.98kDa and 0.15, respectively. However, Fsar's polydispersity index (1.12) and fucose content (34.50%) were lower than those of Fysk, at 1.88 and 43.30%, respectively. Both Fsar and Fysk showed similar thermo-gravimetric properties with four mass losses, amorphous in nature and negative optical rotations. Results show that Fsar has fundamental characteristics of fucoidan with different structural conformation i.e. variation in glycosidic linkages and sulphate group orientation.
    MeSH terms: Fucose/isolation & purification*; Malaysia; Molecular Weight; Magnetic Resonance Spectroscopy; Plant Extracts/chemistry*; Polysaccharides/isolation & purification*; Seaweed/growth & development; Seaweed/chemistry*; Sargassum/growth & development; Sargassum/chemistry*
  18. Ali AA, Hashim AM
    Nanoscale Res Lett, 2016 Dec;11(1):246.
    PMID: 27173675 DOI: 10.1186/s11671-016-1466-x
    We demonstrate a systematic computational analysis of the measured optical and charge transport properties of the spray pyrolysis-grown ZnO nanostructures, i.e. nanosphere clusters (NSCs), nanorods (NRs) and nanowires (NWs) for the first time. The calculated absorbance spectra based on the time-dependent density functional theory (TD-DFT) shows very close similarity with the measured behaviours under UV light. The atomic models and energy level diagrams for the grown nanostructures were developed and discussed to explain the structural defects and band gap. The induced stresses in the lattices of ZnO NSCs that formed during the pyrolysis process seem to cause the narrowing of the gap between the energy levels. ZnO NWs and NRs show homogeneous distribution of the LUMO and HOMO orbitals all over the entire heterostructure. Such distribution contributes to the reduction of the band gap down to 2.8 eV, which has been confirmed to be in a good agreement with the experimental results. ZnO NWs and NRs exhibited better emission behaviours under the UV excitation as compared to ZnO NSCs and thin film as their visible range emissions are strongly quenched. Based on the electrochemical impedance measurement, the electrical models and electrostatic potential maps were developed to calculate the electron lifetime and to explain the mobility or diffusion behaviours in the grown nanostructure, respectively.
  19. Suwankesawong W, Dhippayom T, Tan-Koi WC, Kongkaew C
    Pharmacoepidemiol Drug Saf, 2016 09;25(9):1061-9.
    PMID: 27174034 DOI: 10.1002/pds.4023
    PURPOSE: This study aimed to explore the current landscape and identify challenges of pharmacovigilance (PV) among Association of Southeast Asian Nations (ASEAN) countries.

    METHODS: This cross-sectional survey collected data from May 2014 to December 2015. Questionnaires seeking to collect information on resources, processes, roles and responsibility, and functions of PV systems were sent to relevant persons in the ASEAN countries. Functions of PV centers were measured using the minimum World Health Organization requirements for a functional national PV system. Performances of PV centers were measured by the following: (1) the indicators related to the average number of individual case safety reports (ICSR); (2) presence of signal detection activities and subsequent action; and (3) contribution to the global vigilance database.

    RESULTS: Cambodia, Indonesia, Laos, Malaysia, the Philippines, Singapore, Thailand, and Vietnam completed the survey. PV systems in four surveyed countries (Indonesia, Malaysia, Singapore, and Thailand) achieved all aspects of the World Health Organization minimum requirement for a functional national PV system; the remaining countries were deemed to have unclear communication strategies and/or no official advisory committee. Average numbers of recent ICSR national returns ranged from 7 to 3817 reports/year/million population; three countries (Malaysia, Singapore, and Thailand) demonstrated good performance in reporting system and reported signal detection activities and subsequent actions. All participating countries had submitted ICSRs to the Uppsala Monitoring Center during the survey period (2013-2015).

    CONCLUSIONS: Four participating countries had functional PV systems. PV capacity, functionality, and legislative framework varied depending on local healthcare ecosystem networks. Implementing effective communication strategies and/or technical assistance from the advisory committee are needed to strengthen PV in ASEAN. Copyright © 2016 John Wiley & Sons, Ltd.

    MeSH terms: Asia, Southeastern/epidemiology; Cross-Sectional Studies; Humans; Surveys and Questionnaires; World Health Organization; Databases, Factual; Adverse Drug Reaction Reporting Systems*; Pharmacovigilance*; Drug-Related Side Effects and Adverse Reactions/epidemiology*
  20. Gopalai AA, Ahmad-Annuar A, Li HH, Zhao Y, Lim SY, Tan AH, et al.
    PMID: 27174169 DOI: 10.1002/ajmg.b.32454
    PARK16 was identified as a risk factor for Parkinson's disease in a Japanese cohort; however, subsequent studies in the other populations including the Chinese, European, Caucasian, and Chilean have shown a protective role instead. To investigate this locus in our Malaysian cohort, 1,144 individuals were screened for five SNPs in the PARK16 locus and logistic regression analysis showed that the A allele of the rs947211 SNP reduced the risk of developing PD via a recessive model (Odds ratio 0.57, P-value 0.0003). Pooled analysis with other Asian studies showed that A allele of the rs947211 SNP decreased the risk of developing PD via a recessive model (Odds ratio 0.71, P-value 0.0001). In addition, when meta-analysis was performed with other Asian population, three SNPs (rs823128, rs823156, and rs11240572) reduced risk of developing PD via a dominant model. © 2016 Wiley Periodicals, Inc.
    MeSH terms: Adult; Aged; Aged, 80 and over; Alleles; Ethnic Groups; Female; Humans; Malaysia; Male; Middle Aged; Parkinson Disease/genetics*; Parkinson Disease/metabolism; Parkinson Disease/physiopathology; Risk Factors; Genetic Predisposition to Disease; Polymorphism, Single Nucleotide/genetics; Genome-Wide Association Study; Genetic Association Studies
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