Browse publications by year: 2016

  1. Wong, C.W., Lim, W.T.
    MyJurnal
    The physiochemical characteristics and microbiological study of spray-dried papaya powder
    kept in aluminium laminated polyethylene (ALP) and polyethylene terephthalate (PET) at
    accelerated storage (38 ± 2°C, 90% relative humidity (RH)) for 7 weeks were evaluated. The
    final water activity (Aw) of the spray-dried papaya powder did not exceed 0.6 for both packaging
    materials, which showed that the powder was shelf-stable. The overall adsorbed moisture of
    spray-dried powder was significantly (p
  2. Fahisham Taib, Muhammad Yusoff Mohd Ramdzan, Mavinkurve, Meenal, Subramaniam, Shamini, Mohd Anas Che Nik, Hazama Mohamad, et al.
    MyJurnal
    Recurrent respiratory papillomatosis (RRP) is a rare cause of childhood stridor which typically presents before the age of five years and results from the vertical transmission of the human papilloma virus. Genotypes 11 and 6 are commonly implicated in RRP. Following vertical transmission, the human papilloma virus (HPV) causes overgrowth of the airway epithelium which causes partial airway obstruction and the symptoms of stridor. The mainstay management is surgical debridement of the papillomata but the recurrence rate subsequent to surgery is high, such that most children will require repeated surgical procedures at regular interval. Medical adjuvant therapy can be used, but data is limited and the medications are used on an off-label basis. This case highlights the ethical considerations that need to be made when using off-label medications in paediatric patients.
  3. Kasim R, Draman N, Abdul Kadir A, Muhamad R
    MyJurnal
    Introduction: Pregnancy is common among women in the reproductive age and is associated with several risks. Preconception care has thus been introduced to promote health before conception and to improve pregnancy-related outcomes. The aim of this study was to determine the level of knowledge, attitudes and practices regarding preconception care among women attending antenatal care appointments. Methodology: This was a cross-sectional study conducted from April to December 2012 at Klinik Kesihatan Bachok. A self-administered questionnaire was administered to 135 respondents from 18 to 45 years of age. The questionnaire consisted of four domains, assessing socio-demographic data and knowledge, attitudes and practice of preconception care. Results: The mean (SD) knowledge, attitude and practice scores were 11.37 (3.94), 15.39 (2.12) and 10.13 (2.30), respectively. In total, 98.5% of the respondents had good attitudes, 45.2% had good practices, and 51.9% had good knowledge of preconception care. Conclusion: Women in Bachok have fair knowledge of and good attitude towards preconception care. However, they have poor preconception care practices.
    MeSH terms: Ambulatory Care Facilities; Attitude; Cross-Sectional Studies; Demography; Female; Fertilization; Humans; Malaysia; Pregnancy; Pregnancy Outcome; Prenatal Care; Surveys and Questionnaires; Risk; Preconception Care
  4. Wan-Norafikah, O., Lee, H.L., Norazizah, A., Mohamad-Hafiz, A.
    Trop Biomed, 2016;33(2):396-402.
    MyJurnal
    An indoor ozone-producing air purifier was evaluated for its repellency effects on
    Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, Musca domestica and Periplaneta
    americana adults using a modified tunnel cage. The first set of testing consisted of both bait
    and the air purifier, whereas only bait was offered in the second testing set. The highest
    percent repellency was recorded among Cx. quinquefasciatus (83.23%) while Ae. aegypti
    (68.56%) was the least repelled by the air purifier tested. No mortality was observed in all
    testings. These results illustrated the potential use of the ozone-producing air purifier as a
    personal protection insect repellent device in premises. Nevertheless, in order to prevent
    dengue infection which is transmitted by both Ae. aegypti and Ae. albopictus, ozone-producing
    air purifier should be utilized concurrently with other vector control methods.
    MeSH terms: Aedes; Animals; Culex; Dengue; Houseflies; Insect Repellents; Mosquito Control; Ozone; Periplaneta; Protective Devices; Air Filters
  5. Alazzawi, S., Lynn, E.L.Y., Ibrahim, J., Wee, C.A., Raman, R.
    Trop Biomed, 2016;33(2):393-395.
    MyJurnal
    A study was carried out to investigate the presence of mites in human ear in 58
    patients (113 ears). Ear scrapings were examined under the microscope by a parasitologist
    for the presence of house dust mites. Results showed the presence of house dust mites in 8
    (7.1%) ears. We can conclude that mites are normal commensals of the external ears in
    tropical countries.
    MeSH terms: Allergens; Animals; Ear, External; Environment; Humans; Pyroglyphidae
  6. Valian, F., Sadeghifard, N., Pakzad, I., Valizadeh, N., Karami, S., Badakhsh, B., et al.
    Trop Biomed, 2016;33(2):383-386.
    MyJurnal
    Presently, there is an increase in antibiotic resistance in bacteria, due to relax
    prescription of antibiotics, especially in Iran. Undoubtedly, in toxin antitoxin (TA) system, a
    toxin neutralized by antitoxin, which known as a potent antimicrobial target; but there is no
    extensive survey on the prevalence of TA loci in large scale of Klebsiella pneumoniae.
    Therefore, this study aims to determine the prevalence of different TA loci in clinical and
    environmental K. pneumoniae isolates. For this reason, 48 K. pneumoniae clinical isolates
    and 49 K. pneumoniae environmental isolates were subjected for evaluation of different TA
    loci. The results of current study indicated that there is no association between antibiotic
    resistances and presence of TA loci in clinical and environmental K. pneumoniae. The role of
    TA loci as a potent target in antibiotic resistant K. pneumoniae has been complicated.
    Therefore, more studies should be performed to explain why TA loci are presented in K.
    pneumoniae and what is the rationale behind antibiotic resistant K. pneumoniae?
    MeSH terms: Anti-Infective Agents; Anti-Bacterial Agents; Antitoxins; Drug Resistance, Microbial; Iran; Klebsiella pneumoniae; Surveys and Questionnaires; Toxins, Biological; Prevalence
  7. Wahizatul, A.A., Faridah, M., Nur Farhah, A.S.
    Trop Biomed, 2016;33(2):359-365.
    MyJurnal
    Musca domestica Linnaeus or house fly is one of the well-known cosmopolitan
    pests. Poultry farms, horse stables and ranches are some of the main habitats for house flies
    to extend their interference and disturbance. Since 1999, large number of fly population
    around horse stables and barns in Terengganu Equestrian Resort (TER) was detected and still
    persists till today. Imported horse food are speculated to be the main cause for the increasing
    number of these flies. The current study was conducted to determine the effect of imported
    horse food (i.e. chaff, pallet and alfalfa) and the food conditions (wet and dry) on house fly
    population densities. Commercial sticky papers were used for trapping house fly distribution
    around the horse stables and barns in TER from October 2013 to January 2014. A total of
    36,751 flies were caught during the eight week study period. The highest number of flies
    (2,843 individuals) was caught when using ‘wet alfalfa + chaff’ whereas the lowest catch was
    when using ‘dry alfalfa’ (1,652 individuals). Generally, the wet food treatments captured
    higher flies compared to dry food treatments. The total number of captured flies was found to
    be influenced by relative humidity. Higher mean number of flies was captured during lower
    weekly humidity. Our findings conclude that the wet condition of imported horse food in TER
    increases the house flies population densities. Thus, urgent action should be taken as soon as
    possible and may need special management considerations in order to reduce the impact of
    house flies on the horse industry in Malaysia.
    MeSH terms: Medicago sativa; Animals; Diptera; Horses; Houseflies; Humans; Humidity; Malaysia; Population Density; Poultry; Ecosystem; Farms
  8. Nur Liyana, K., Fauziah, M.K., Zainah, S., Nor Aziyah, R., Salbiah, N., Lau, I.S., et al.
    Trop Biomed, 2016;33(2):348-358.
    MyJurnal
    Dengue is a mosquito-borne viral disease caused by four serotypes of dengue
    virus, affecting the human population for decades in many tropical and subtropical regions of
    the world. In Malaysia, all four dengue serotypes co-circulates in a dengue season even
    though any one of the serotypes can predominate. In this study, serum samples were collected
    from dengue fever and severe dengue fever patients within Klang Valley from 2010-2012 to
    determine the prevailing dengue serotypes. In addition, sequencing of the envelope/nonstructural
    1 (E/NS1) gene junction of the virus isolated was performed to identify the presence
    of any mutations that are suggestive of increased virulence in the virus. The results showed
    that Dengue-1 (DEN-1) was the predominant circulating serotype. The E/NS1 gene sequences
    of the isolates were analysed to trace the evolutionary knowledge of the strains. All sequences
    of the isolates were compared with DEN-1 prototype Hawaii strain as the reference sequence.
    The E/NS1 sequences of other dengue strains from neighbouring regions as well as other
    parts of the world obtained from the GenBank database were also included in the phylogenetic
    tree analysis. Analyses showed that there was 97% to 100% similarity among the ten isolates
    at the nucleotide level. Similarly, the amino acid analogue also showed 98% to 100% homology.
    However, all five non-severe dengue isolates showed variation at position 780, resulting in an
    amino acid change from valine to alanine as compared to severe dengue isolates. A rooted
    phylogenetic tree was performed using neighbour-joining method with DEN-2 and DEN-3 as
    the outgroups. Results showed that all ten isolates were classified as genotype I. In addition,
    the five isolates from severe dengue patients were found to be clustered together with
    JN697057 and JN697058, Malaysian DEN-1 strains from the 2005 outbreak.
    MeSH terms: Alanine; Dengue; Dengue Virus; Disease Outbreaks; Genotype; Hawaii; Humans; Malaysia; Culicidae; Mutation; Nucleotides; Phylogeny; Seasons; Valine; Virulence; Genes, env; Severe Dengue; Databases, Nucleic Acid; Serogroup
  9. Hamzah, S.N., Alias, Z.
    Trop Biomed, 2016;33(2):335-347.
    MyJurnal
    Glutathione S-transferases (GSTs) are multifunctional enzymes involved in
    detoxification of xenobiotic compounds in many insect groups. This work investigated the
    expression of GSTs in Aedes albopictus by using affinity chromatography and proteomic
    analysis. The study compared the expression of GSTS isoforms in larvae of three Ae. albopictus
    strains (susceptible (VCRU) strain, field Kuala Lumpur (KL) strain and laboratory permethrintreated
    strain). The study demonstrated that the expression of sixteen isoforms (GSTS1-1,
    GSTS1-4, GSTS1-3, GSTS1-4, GSTD1-3, GSTD1-2, GSTD1-5, GSTD1-6, GSTD1-7, GSTD1-8,
    GSTD1-9, GSTD1-10, GSTD1-11, GSTT, GSTD1-12, and GSTD1-13) were significantly increased
    in the field KL strain (p0.05).
    However, while comparing the expression of GST isoforms in field KL larvae and laboratory
    permethrin-treated strain, the same pattern was observed for all the GSTs especially for
    Theta and Sigma class (p>0.05). In Delta class, only GSTD1-1, GSTD1-9, GSTD1-11, GSTD1-
    12 and GSTD1-13 shows significant difference in expression between these two strain (p
    MeSH terms: Aedes; Animals; Chondroitin Sulfates; Chromatography, Affinity; Disaccharides; Glutathione Transferase; Larva; Inactivation, Metabolic; Protein Isoforms; Permethrin; Proteomics; Multifunctional Enzymes
  10. Kuan, C.S., Hooi, P.S., Lee, L.M., Sam, I.C., Ng, K.P.
    Trop Biomed, 2016;33(2):311-319.
    MyJurnal
    Early detection of viral etiologies of acute respiratory tract infections of patients
    affects management and disease control in pediatric patients. In this study, the performance
    of Anyplex II RV16 assay (Seegene, Seoul, Korea) was evaluated by comparing with viral
    culture and direct immunofluorescence staining of clinical specimens for detection of
    respiratory viruses in patients. A total of 168 respiratory specimens were collected from 122
    patients from November 2012 to May 2013 at the time of admission to the University of
    Malaya Medical Centre (UMMC), Kuala Lumpur, Malaysia. The Anyplex II RV16 assay, viral
    culture, and direct immunofluorescence staining were positive in 74.4%, 18.5% and 14.9% of
    the specimens, respectively. HRV was the predominant virus detected by the Anyplex II RV16
    assay. In 47 cases, two or more respiratory viruses were detected by the Anyplex II RV16
    assay, which were missed by conventional methods. The performance of the Anyplex II RV16
    assay was better than viral culture and direct immunofluorescence staining of clinical
    specimens for the detection of respiratory viruses. The implementation of the Anyplex II
    RV16 assay in hospital laboratories will provide rapid diagnosis of major viral infections of the
    respiratory tract.
    MeSH terms: Child; Humans; Laboratories, Hospital; Malaysia; Respiratory System; Respiratory Tract Infections; Staining and Labeling; Virus Diseases; Viruses; Fluorescent Antibody Technique, Direct; Republic of Korea; Seoul
  11. Khor GL, Tan SY, Tan KL, Chan PS, Amarra MS
    Nutrients, 2016 Dec 01;8(12).
    PMID: 27916932
    BACKGROUND: The 2010 World Health Organisation (WHO) Infant and Young Child Feeding (IYCF) indicators are useful for monitoring feeding practices.

    METHODS: A total sample of 300 subjects aged 6 to 23 months was recruited from urban suburbs of Kuala Lumpur and Putrajaya. Compliance with each IYCF indicator was computed according to WHO recommendations. Dietary intake based on two-day weighed food records was obtained from a sub-group (N = 119) of the total sample. The mean adequacy ratio (MAR) value was computed as an overall measure of dietary intake adequacy. Contributions of core IYCF indicators to MAR were determined by multinomial logistic regression.

    RESULTS: Generally, the subjects showed high compliance for (i) timely introduction of complementary foods at 6 to 8 months (97.9%); (ii) minimum meal frequency among non-breastfed children aged 6 to 23 months (95.2%); (iii) consumption of iron-rich foods at 6 to 23 months (92.3%); and minimum dietary diversity (78.0%). While relatively high proportions achieved the recommended intake levels for protein (87.4%) and iron (71.4%), lower proportions attained the recommendations for calcium (56.3%) and energy (56.3%). The intake of micronutrients was generally poor. The minimum dietary diversity had the greatest contribution to MAR (95% CI: 3.09, 39.87) (p = 0.000) among the core IYCF indicators.

    CONCLUSION: Malaysian urban infants and toddlers showed moderate to high compliance with WHO IYCF indicators. The robustness of the analytical approach in this study in quantifying contributions of IYCF indicators to MAR should be further investigated.

    MeSH terms: Breast Feeding/ethnology; Child Day Care Centers; Child Development; Cross-Sectional Studies; Diet/adverse effects*; Diet/ethnology; Feeding Methods/adverse effects*; Female; Health Status Indicators; Humans; Infant; Malaysia; Male; Nutrition Surveys; Parents; World Health Organization; Diet Records; Caregivers; Nutrition Policy*
  12. Lee, Elaine Lim Siew, Nur Arzuar Abdul Rahim, Sharifah Azdiana Tuan Din
    MyJurnal
    Introduction: Nurses are generally trained on the procedures of blood transfusion during their years of service. However, the level of knowledge varies among individuals and healthcare centres. Thus, this study addresses a knowledge gap regarding nurses’ knowledge and transfusion safety in the local clinical setting. Objectives: This study aimed to determine nurses’ level of knowledge of various phases of blood transfusion and associate this knowledge with their socio-professional details. Methods: This was a cross-sectional, quantitative study. The target population was in-patient ward nurses at Hospital Pulau Pinang. The systematic random sampling method was used to select 185 nurses to participate in this study. Data were collected using a validated research questionnaire that consisted of 31 items (9 items related to socio-professional factors and 22 knowledge items). Data were analysed using descriptive and inferential analyses. Results: The overall knowledge score was moderate (mean = 70.44%, SD = 11.35). None of the nurses was able to answer all of the knowledge questions correctly. Of the participants, 72.29%, 71.75%, and 67.14% exhibited knowledge of blood bag collection and patient preparation; pre-transfusion nursing activities; and during and post-transfusion nursing responsibilities and management of transfusion reactions, respectively. Factors such as age, years of service, department, and availability of the transfusion policy in the ward significantly affected the mean knowledge scores. Conclusion: Nurses’ knowledge of blood transfusion at Hospital Pulau Pinang was moderate. More training and courses should be provided to improve their knowledge.
  13. Asha V, Devi V, Raghavendra R, Rachel AR, Vinod P, Sharmila T
    MyJurnal
    Objectives: This investigation focused on finding the changes in learning approaches of students’ while experiencing physiology curriculum and to explore the reflection of changes if any, on their critical thinking skills.
    Methods: In this longitudinal study, information on students’ learning approaches was obtained using the revised two factor study process questionnaire (R-SPQ-2F) at the commencement (pre-test) and completion (post-test) of first year MBBS course. The total score and the scores obtained in the critical thinking questions in four physiology assessments were analysed. Pre and post-tests scores in R-SPQ-2F and assessment scores were compared using independent samples t-test and one way repeated measure ANOVA respectively. Correlation of scores between assessments was done using Pearson correlation. A p value < 0.05 was taken as significant.
    Results: A significant increase in students’ deep learning approach at the completion of the physiology curriculum when compared to commencement (p < 0.001) was observed. A progressive improvement in their scores as they experienced the curriculum was also noticed. A moderate correlation among critical thinking questions scores and a strong correlation between critical thinking questions and total essay scores were also recorded.
    Conclusion: The study revealed improvement in students’ deep approach to learning in physiology which was reflected in their performance in critical thinking questions.
    Study site: Melaka Manipal Medical College, Manipal University, India
    MeSH terms: Humans; India; Prospective Studies; Students, Medical*; Thinking; Universities
  14. Muhammad Hibatullah Roml, Farahiyah Wan Yunus
    MyJurnal
    Introduction: Investigation on spiritual issue in healthcare practice received less attention. Muslims valued pray activity or known as salat as one of the important daily activity. However, negligible study is available on salat as the end goal of occupational therapy intervention. This study aims to investigate the awareness and the impact of Muslim-culture on occupational therapists in Malaysia. Methods: A cross-sectional survey study was conducted via internet-based and manual paper-and-pencil survey throughout occupational therapists in Malaysia. Descriptive and inferential statistics using percentage, Cronbach’s alpha and Chi-square was implemented on the findings. Results: In total, 119 occupational therapists responded. The questionnaire has overall internal consistency ofα = 0.74. Occupational therapists have positive perceptions on spiritual activity but poorly translated into service implementation and faced challenges. Factors such as level of education, years of experience, religion, working location and work setting were significant on the awareness, perception, practice and challenges. Cultural-based practice is challenging for occupational therapy even in the majority-practice context. Occupational therapists should differentiate between belief and activity in spiritual issue to provide a better assistance for the clients. Conclusion: This study showed continuing education is desired to drive the occupational therapy profession for a better cultural-sensitivity service delivery.
    MeSH terms: Adult; Cross-Sectional Studies; Humans; Islam; Malaysia; Religion; Faith Healing; Occupational Therapists
  15. Harlina Halizah Siraj, Abdus Salam, Verasingam, Jaarvis, Syatirah Jani, Yuen, Ling Chung, Gue, Kay Lyn, et al.
    MyJurnal
    Mastering the art of conducting a research along with the competitive era of publishing papers showed the importance of undergraduate research. The purpose of this research was to study the impact of undergraduate research “Special Study Module (SSM)” on Universiti Kebangsaan Malaysia (UKM) medical students and alumni.
    Methodology: This was a cross-sectional study whereby 64 fifth year UKM medical students of academic-session 2014/2015 and 49 alumni of year 2010 were participated on their SSM research activities. The questionnaire administered online which enquired on participants’ level of interest, confidence and SSM research experiences. Frequency and percentage distribution were used for descriptive analysis.
    Result: Out of 119 participants,113 responded giving a response rate of 95%. More than 90% respondents in both group felt that SSM experience was enriching and 80%–90% felt SSM experience was pleasant. In both groups 47%–50% respondents believed, SSM experience contributed their high level of interest on research. The most influencing factors on their level of interest were supervisor guidance as reported by73%–80% respondents. It was said that SSM supervisor was academically stimulating, impressive as role model and supportive. The most confidence skill was the ability to function within a group and least confidence skill was manuscript writing.
    Conclusion: Undergraduate SSM research showed positive impact on participants’ research activities. More in-depth analysis should be considered especially on manuscript writing as the students were least confident in this skill.
    MeSH terms: Adult; Cross-Sectional Studies; Humans; Malaysia; Research; Students, Medical; Universities
  16. Khor, Kuan Hua, Tan, Wei Xian, Lau, Seng Fong, Mohd Azri Roslan, Rozanaliza Radzi, Siti Khairani Bejo, et al.
    Trop Biomed, 2016;33(2):276-284.
    MyJurnal
    A study on seroprevalence and molecular detection of canine leptospirosis was
    carried out in a dog population (randomly selected n=80 dogs) from an animal shelter X. All
    the dogs in Shelter X appeared clinically healthy. Eighty blood samples were obtained and
    their serum were serologically examined using Microscopic Agglutination Test (MAT) against
    10 Leptospira serovars. Plasma samples obtained were subjected to Polymerase Chain Reaction
    (PCR) assay. Three out of 80 dogs (3.8%) tested positive for L. bataviae based on MAT at a
    titer of 1:80. The seroprevalence of 9 other Leptospira serovars was not evident in this study.
    All the dogs tested negative against leptospirosis with PCR assay. In conclusion, canine
    leptospirosis was detected in dogs in this animal shelter. L. bataviae was identified as the
    infecting serovar. To our knowledge, this is the second report of serovar Bataviae infection in
    dogs in Malaysia. The 3 dogs in our study could possibly be a source of leptospiral infection
    to other dogs and may shed the bacteria into the environment. This serovar is not available in
    canine vaccination programs, therefore the dogs are not protected from this disease. Further
    investigation is warranted to determine whether the infected dogs are carriers of this serovar.
    MeSH terms: Agglutination Tests; Animals; Bacteria; Dogs; Leptospira; Leptospirosis; Malaysia; Vaccination; Seroepidemiologic Studies; Polymerase Chain Reaction; Serogroup
  17. Aw-Yong, K.L., Tan, C.W., Sam, I.C., Chan, Y.F., Koh, M.T.
    Trop Biomed, 2016;33(2):238-245.
    MyJurnal
    Hand, foot and mouth disease (HFMD) is a common childhood infection caused by
    many enteroviruses, including enterovirus A71 (EV-A71). As EV-A71 is associated with severe
    neurological disease, early diagnosis is critical for clinical and public health management. In
    developing countries such as Malaysia, laboratory capacity to carry out EV-A71 IgM detection
    is greater than that of the gold standard methods of virus culture or molecular detection. This
    study evaluated two diagnostic kits, EV-A71 IgM-capture enzyme-linked immunosorbent
    (ELISA) and EV-A71 IgM-colloidal gold immunochromatographic assay (GICA), which had
    previously only been assessed in China. The assays were tested with 89 serum samples from
    patients with suspected HFMD. The sensitivity, specificity, positive predictive value, and
    negative predictive value rates were 78.4%, 80.8%, 74.4%, and 84.0%, respectively, for the
    IgM-capture ELISA, and 75.7%, 76.9%, 70.0%, and 81.6% for the IgM GICA. These performance
    measures were similar between the two assays. Concordance between the two assays was
    91.1%. The sensitivity rates were lower than those previously reported, likely because the
    multiple circulating EV-A71 genotypes in Malaysia differ from the C4 subgenotype found in
    China and used in the assays. Both assays had low false positive rates (12.5% and 16.7% for
    ELISA and GICA, respectively) when tested on sera from patients confirmed to have
    enteroviruses. Both diagnostic kits are suitable for early diagnosis of HFMD caused by EVA71
    in Malaysia, but confirmation with culture or PCR is still important.
    MeSH terms: China; Developing Countries; Enterovirus Infections; Enterovirus; Genotype; Hand, Foot and Mouth Disease; Humans; Immunoglobulin M; Immunosorbents; Malaysia; Public Health; Sensitivity and Specificity; Polymerase Chain Reaction; Gold Colloid; Early Diagnosis; Immunochromatography
  18. Siti Halimah Hasmoni, Goh, Kian Mau, Shafinaz Shahir, Saiful Anuar Karsani
    Trop Life Sci Res, 2016;27(1):63-75.
    MyJurnal
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  19. Zainal Baharum, Abdah Md Akim, Roslida Abdul Hamid, Taufiq Yap Yun Hin, Rosmin Kasran
    Trop Life Sci Res, 2016;27(1):21-42.
    MyJurnal
    Plants have been a good source of therapeutic agents for thousands of years;
    an impressive number of modern drugs used for treating human diseases are derived from
    natural sources. The Theobroma cacao tree, or cocoa, has recently garnered increasing
    attention and become the subject of research due to its antioxidant properties, which are
    related to potential anti-cancer effects. In the past few years, identifying and developing
    active compounds or extracts from the cocoa bean that might exert anti-cancer effects
    have become an important area of health- and biomedicine-related research. This review
    provides an updated overview of T. cacao in terms of its potential anti-cancer compounds
    and their extraction, in vitro bioassay, purification, and identification. This article also
    discusses the advantages and disadvantages of the techniques described and reviews the
    processes for future perspectives of analytical methods from the viewpoint of anti-cancer
    compound discovery.
    MeSH terms: Antioxidants; Attention; Biological Assay; Cacao; Humans; Trees; Biomedical Research; Chocolate
  20. V-Ghaffari B, Kouhnavard M, Kitajima T
    J Biol Syst, 2016 Dec;24(4):561-575.
    PMID: 28356608 DOI: 10.1142/S0218339016500285
    Subthreshold-level activities in neurons play a crucial role in neuronal oscillations. These small-amplitude oscillations have been suggested to be involved in synaptic plasticity and in determining the frequency of network oscillations. Subthreshold membrane oscillations (STOs) and subthreshold resonance oscillations (SROs) are the main constituents of subthreshold-level activities in neurons. In this study, a general theoretical framework for analyzing the mechanisms underlying STOs and SROs in neurons is presented. Results showed that the resting membrane potential and the hyperpolarization-activated potassium channel (h-channel) affect the subthreshold-level activities in stellate cells. The contribution of h-channel on resonance is attributed to its large time constant, which produces the time lag between Ih and the membrane potential. Conversely, the persistent sodium channels (Nap-channels) only play an amplifying role in these neurons.
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