Browse publications by year: 2016

  1. Nurnazahiah A, Lua PL, Shahril MR
    Malays J Med Sci, 2016 Nov;23(6):7-24.
    PMID: 28090175 MyJurnal DOI: 10.21315/mjms2016.23.6.2
    The objective of this study was to compile and analyse existing scientific evidences reporting the effects of objectively measured physical activity on the levels of adiponectin and leptin. Articles related to the effects of objectively measured physical activity on the levels of adiponectin and leptin were searched from the Medline and PubMed databases. The search was limited to 'objectively measured' physical activity, and studies that did not objectively measure the physical activity were excluded. Only English articles were included in the search and review. A total of 18 articles encompassing 2,026 respondents met the inclusion criteria. The eligible articles included all forms of evidence (e.g., cross-sectional and intervention). Seventeen and 11 studies showed the effects of objectively measured physical activity on adiponectin and leptin, respectively. Five and four cross-sectional studies showed the effects of objectively measured physical activity on adiponectin and leptin, respectively. Two out of five studies showed a weak to moderate positive association between adiponectin and objectively measured physical activity, while three out of four studies showed a weak to moderate inverse association between leptin and objectively measured physical activity. For intervention studies, six out of 12 studies involving adiponectin and five out of seven studies involving leptin showed a significant effect between the proteins and objectively measured physical activity. However, a definitive conclusion could not be drawn due to several methodological flaws in the existing articles and the acute lack of additional research in this area. In conclusion, the existing evidences are encouraging but yet not compelling. Hence, further well-designed large trials are needed before the effectiveness of objectively measured physical activity in elevating adiponectin levels and in decreasing leptin levels could be strongly confirmed.
    MeSH terms: Compulsive Behavior; Cross-Sectional Studies; Surveys and Questionnaires; Exercise; MEDLINE; Leptin; PubMed; Adiponectin
  2. Abd Wahab F, Abdullah S, Abdullah JM, Jaafar H, Md Noor SS, Wan Mohammad WMZ, et al.
    Malays J Med Sci, 2016 Nov;23(6):25-34.
    PMID: 28090176 MyJurnal DOI: 10.21315/mjms2016.23.6.3
    Ranking as the most communicable disease killer worldwide, tuberculosis, has accounted with a total of 9.6 million new tuberculosis cases with 1.5 million tuberculosis-related deaths reported globally in 2014. Tuberculosis has remain as an occupational hazard for healthcare workers since 1920s and due to several tuberculosis outbreaks in healthcare settings in the early 1990s, the concern about the transmission to both patients and healthcare workers has been raised. Healthcare workers have two to three folds greater the risk of active tuberculosis than the general population. Several studies on knowledge, attitude and practices on tuberculosis among healthcare workers worldwide have revealed that majority of the participated healthcare workers had good knowledge on tuberculosis. Most of the healthcare workers from South India and South Africa also reported to have positive attitude whereas a study in Thailand reported that most of the healthcare providers have negative attitude towards tuberculosis patients. Nevertheless, majority of the healthcare workers have low level of practice on tuberculosis prevention. An improved communication between healthcare workers and the patients as well as their families is the key to better therapeutic outcomes with good knowledge, attitude and preventive practice towards tuberculosis.
    MeSH terms: Communicable Diseases; Disease Outbreaks; Health Personnel; Humans; India; Risk; South Africa; Thailand; Tuberculosis
  3. Asyikeen WA, Siti-Azrin AH, Jalil NA, Zin AA, Othman NH
    Malays J Med Sci, 2016 Nov;23(6):44-51.
    PMID: 28090178 MyJurnal DOI: 10.21315/mjms2016.23.6.5
    Endometrial cancer is the most common gynaecologic malignancy among females worldwide. The purpose of this study was to determine the median survival time of endometrial cancer patients at the Hospital Universiti Sains Malaysia (USM).
    MeSH terms: Female; Genital Neoplasms, Female; Hospitals; Humans; Malaysia; Endometrial Neoplasms
  4. Othman MA, Ghazali WS, Yahya NK, Wong KK
    Malays J Med Sci, 2016 Nov;23(6):52-59.
    PMID: 28090179 MyJurnal DOI: 10.21315/mjms2016.23.6.6
    BACKGROUND: The rheumatoid factor (RF) blood test is the most commonly adopted test for the diagnosis of rheumatoid arthritis (RA). RA patients who are seropositive for RF might face a greater likelihood of developing more aggressive symptoms.

    METHODS: Our goal was to study the demographic and clinical characteristics, as well as their correlation with RF seropositivity, among a series of 80 RA patients aged ≥ 18 years who attend Hospital Universiti Sains Malaysia (HUSM).

    RESULTS: Of the 80 RA patients included in this study, 66 (82.5%) were female and 14 (17.5%) were male. No significant associations between RF seropositivity and demographic and/or clinical characteristics or other laboratory investigations were observed, including gender, morning stiffness, individual joint involvement (from multiple sites of the body), and erythrocyte sedimentation rate (ESR) measurement. However, a significant association between RF seropositivity and patients aged ≥ 50 was found (P = 0.032).

    CONCLUSION: RF seropositivity was found to be more common in much older RA patients.

  5. Hassan AB, Begum T, Reza MF, Yusoff N
    Malays J Med Sci, 2016 Nov;23(6):70-82.
    PMID: 28090181 MyJurnal DOI: 10.21315/mjms2016.23.6.8
    Previous studies have revealed that self-related tasks (items) receive more attention than non-self-related, and that they elicit event-related potential (ERP) components with larger amplitudes. Since personality has been reported as one of the biological correlates influencing these components, as well as our behavioural differences, it is important to examine how it affects our self-consciousness in relation to tasks of varied relevance and the neurological basis.
    MeSH terms: Attention; Consciousness; Evoked Potentials; Personality; Personality Disorders
  6. Ab Ghani A, Nayan SA, Kandasamy R, Rosman AK, Ghani AR
    Malays J Med Sci, 2016 Nov;23(6):113-117.
    PMID: 28090185 MyJurnal DOI: 10.21315/mjms2016.23.6.12
    The annual incidence of intracranial aneurysm in Malaysia is estimated to be 1.1-1.7 per 100,000 population based on a study done conducted in 1988. Since then, little epidemiological research has been conducted in Malaysia, and the real incidence is therefore probably unreported despite advancements in the diagnosis and treatment of this disorder. Intracranial aneurysm may be treated by microsurgical clipping or embolisation depending on its location and the surgeon's preference. This study aims to report the characteristics and outcomes of patients with a clipped anterior circulation aneurysm in Hospital Sungai Buloh.
    MeSH terms: Intracranial Aneurysm; Embolization, Therapeutic; Humans; Malaysia; Surgical Instruments; Incidence; Epidemiologic Studies; Surgeons
  7. Swamy MK, Akhtar MS, Sinniah UR
    PMID: 28090211 DOI: 10.1155/2016/3012462
    A wide range of medicinal and aromatic plants (MAPs) have been explored for their essential oils in the past few decades. Essential oils are complex volatile compounds, synthesized naturally in different plant parts during the process of secondary metabolism. Essential oils have great potential in the field of biomedicine as they effectively destroy several bacterial, fungal, and viral pathogens. The presence of different types of aldehydes, phenolics, terpenes, and other antimicrobial compounds means that the essential oils are effective against a diverse range of pathogens. The reactivity of essential oil depends upon the nature, composition, and orientation of its functional groups. The aim of this article is to review the antimicrobial potential of essential oils secreted from MAPs and their possible mechanisms of action against human pathogens. This comprehensive review will benefit researchers who wish to explore the potential of essential oils in the development of novel broad-spectrum key molecules against a broad range of drug-resistant pathogenic microbes.
  8. Kim SH, Tan KL, Lee SY, Kim DW, Shin S, Jin HR
    Springerplus, 2016;5(1):2116.
    PMID: 28090430 DOI: 10.1186/s40064-016-3679-y
    This study investigated on bacterial contamination of the rhinoplasty field. The effect of preoperative chlorhexidine treatment on decreasing bacterial contamination in the rhinoplasty field is examined.
  9. Tan KL, Pezzella F
    Oncol Lett, 2016 Dec;12(6):4287-4296.
    PMID: 28101194 DOI: 10.3892/ol.2016.5232
    The capabilities of tumour cells to survive through deregulated cell cycles and evade apoptosis are hallmarks of cancer. The ubiquitin-like proteins (UBL) proteasome system is important in regulating cell cycles via signaling proteins. Deregulation of the proteasomal system can lead to uncontrolled cell proliferation. The Skp, Cullin, F-box containing complex (SCF complex) is the predominant E3 ubiquitin ligase, and has diverse substrates. The ubiquitin ligase activity of the SCF complexes requires the conjugation of neural precursor cell expressed, developmentally down-regulated 8 (NEDD8) to cullin proteins. A tumour suppressor and degrading enzyme named NEDD8 ultimate buster 1 (NUB1) is able to recruit HLA-F-adjacent transcript 10 (FAT10)- and NEDD8-conjugated proteins for proteasomal degradation. Ubiquitination is associated with neddylation and FAT10ylation. Although validating the targets of UBLs, including ubiquitin, NEDD8 and FAT10, is challenging, understanding the biological significance of such substrates is an exciting research prospect. This present review discusses the interplay of these UBLs, as well as highlighting their inhibition through NUB1. Knowledge of the mechanisms by which NUB1 is able to downregulate the ubiquitin cascade via NEDD8 conjugation and the FAT10 pathway is essential. This will provide insights into potential cancer therapy that could be used to selectively suppress cancer growth.
    MeSH terms: Cell Cycle; Neoplasms; Ubiquitins; Down-Regulation; Apoptosis; Ubiquitin; Ubiquitin-Protein Ligases; Cullin Proteins; Proteasome Endopeptidase Complex; Cell Proliferation; Ubiquitination
  10. Sia SF, Zhao X, Li R, Zhang Y, Chong W, He L, et al.
    Proc Inst Mech Eng H, 2016 Nov;230(11):1051-1058.
    PMID: 28095764 DOI: 10.1177/0954411916671752
    BACKGROUND: Internal carotid artery stenosis requires an accurate risk assessment for the prevention of stroke. Although the internal carotid artery area stenosis ratio at the common carotid artery bifurcation can be used as one of the diagnostic methods of internal carotid artery stenosis, the accuracy of results would still depend on the measurement techniques. The purpose of this study is to propose a novel method to estimate the effect of internal carotid artery stenosis on the blood flow based on the concept of minimization of energy loss.

    METHODS: Eight internal carotid arteries from different medical centers were diagnosed as stenosed internal carotid arteries, as plaques were found at different locations on the vessel. A computational fluid dynamics solver was developed based on an open-source code (OpenFOAM) to test the flow ratio and energy loss of those stenosed internal carotid arteries. For comparison, a healthy internal carotid artery and an idealized internal carotid artery model have also been tested and compared with stenosed internal carotid artery in terms of flow ratio and energy loss.

    RESULTS: We found that at a given common carotid artery bifurcation, there must be a certain flow distribution in the internal carotid artery and external carotid artery, for which the total energy loss at the bifurcation is at a minimum; for a given common carotid artery flow rate, an irregular shaped plaque at the bifurcation constantly resulted in a large value of minimization of energy loss. Thus, minimization of energy loss can be used as an indicator for the estimation of internal carotid artery stenosis.

    MeSH terms: Aged; Biomechanical Phenomena; Carotid Artery, Internal/physiopathology; Humans; Male; Models, Cardiovascular; Regional Blood Flow; Carotid Stenosis/physiopathology*; Hydrodynamics
  11. Gomathysankar S, Halim AS, Yaacob NS, Noor NM, Mohamed M
    J Stem Cells Regen Med, 2016;12(2):79-86.
    PMID: 28096632
    Adipose-derived stem cells (ASCs) have potential applications in the repair and regeneration of various tissues and organs. The use of various scaffold materials as an excellent template for mimicking the extracellular matrix to induce the attachment and proliferation of different cell types has always been of interest in the field of tissue engineering because ideal biomaterials are in great demand. Chitosan, a marine polysaccharide, have wide clinical applications and it acts as a promising scaffold for cell migration and proliferation. ASCs, with their multi-differentiation potential, and chitosan, with its great biocompatibility with ASCs, were investigated in the present study. ASCs were isolated and were characterized by two different methods: immunocytochemistry and flow cytometry, using the mesenchymal stem cell markers CD90, CD105, CD73 and CD29. The ASCs were then induced to differentiate into adipogenic, osteogenic and chondrogenic lineages. These ASCs were incorporated into a porous chitosan scaffold (PCS), and their structural morphology was studied using a scanning electron microscope and hematoxylin and eosin staining. The proliferation rate of the ASCs on the PCS was assessed using a PrestoBlue viability assay. The results indicated that the PCS provides an excellent template for the adhesion and proliferation of ASCs. Thus, this study revealed that PCS is a promising biomaterial for inducing the proliferation of ASCs, which could lead to successful tissue reconstruction in the field of tissue engineering.
  12. Moghbel M, Mashohor S, Mahmud R, Saripan MI
    EXCLI J, 2016;15:500-517.
    PMID: 28096782 DOI: 10.17179/excli2016-473
    Segmentation of the liver from Computed Tomography (CT) volumes plays an important role during the choice of treatment strategies for liver diseases. Despite lots of attention, liver segmentation remains a challenging task due to the lack of visible edges on most boundaries of the liver coupled with high variability of both intensity patterns and anatomical appearances with all these difficulties becoming more prominent in pathological livers. To achieve a more accurate segmentation, a random walker based framework is proposed that can segment contrast-enhanced livers CT images with great accuracy and speed. Based on the location of the right lung lobe, the liver dome is automatically detected thus eliminating the need for manual initialization. The computational requirements are further minimized utilizing rib-caged area segmentation, the liver is then extracted by utilizing random walker method. The proposed method was able to achieve one of the highest accuracies reported in the literature against a mixed healthy and pathological liver dataset compared to other segmentation methods with an overlap error of 4.47 % and dice similarity coefficient of 0.94 while it showed exceptional accuracy on segmenting the pathological livers with an overlap error of 5.95 % and dice similarity coefficient of 0.91.
    MeSH terms: Attention; Liver Diseases; Lung; Ribs; Tomography, X-Ray Computed; Cone-Beam Computed Tomography
  13. Loong TY, Chong DL, Jamal AR, Murad NA, Sabudin RZ, Fun LC
    EXCLI J, 2016;15:630-635.
    PMID: 28096792 DOI: 10.17179/excli2016-613
    Haemoglobin (Hb)-M Hyde Park, also known as Hb-M Akita is a rare type of hereditary Hb M due to autosomal dominant mutation of CAC>TAC on codon 92 of β globin gene resulting in the replacement of histidine by tyrosine on β globin chain. This variant Hb has a tendency to form methaemoglobin (metHb). The iron ion in metHb is oxidized to ferric (Fe3+) which is unable to carry oxygen and the patients manifest as cyanosis clinically. A 9-year-old Malay girl was incidentally found to be cyanotic when she presented to a health clinic. Laboratory investigations revealed raised methaemoglobin levels and Hb analysis findings were consistent with Hb-M Hyde Park. β gene sequencing confirmed a point mutation of CAC>TAC on codon 92 in one of the β genes. The family study done on the individuals with cyanosis showed similar findings. A diagnosis of heterozygous Hb-M Hyde Park was made. Patients with this variant Hb usually presented with cyanosis with mild haemolysis and maybe misdiagnosed as congenital heart disease. No further treatment is needed as patients are relatively asymptomatic. Although the disease is harmless in the heterozygous carriers but the offspring of the carriers may suffer severe haemolytic anaemia when the offspring also inherit other β haemoglobinopathies/thalassemia. This can happen due to high prevalence of β thalassemia carrier (3.5-4 %) found in Malaysia. At the time of writing, this is the first case of hereditary Hb-M Hyde Park diagnosed in a Malay family living in Malaysia.
    MeSH terms: Child; Cyanosis; Female; Hemoglobin M; Hemoglobinopathies; Hemolysis; Histidine; Humans; Iron; Malaysia; Methemoglobin; Oxygen; Tyrosine; Prevalence; beta-Thalassemia; Point Mutation; beta-Globins
  14. Tavakoli A, Hamzah A, Rabu A
    Mol Biol Res Commun, 2016 Sep;5(3):133-142.
    PMID: 28097167
    In this study, benzoate dioxygenase from Rhodococcus ruber UKMP-5M was catalyzed by oxidating the benzene ring to catechol and other derivatives. The benzoate dioxygenase (benA gene) from Rhodococcus ruber UKMP-5M was then expressed, purified, characterized, The benA gene was amplified (642 bp), and the product was cloned into a pGEM-T vector. The recombinant plasmid pGEMT-benA was digested by double restriction enzymes BamHI and HindIII to construct plasmid pET28b-benA and was then ligated into Escherichia coli BL21 (DE3). The recombinant E. coli was induced with 0.5 mM isopropyl β-D-thiogalactoside (IPTG) at 22˚C to produce benzoate dioxygenase. The enzyme was then purified by ion exchange chromatography after 8 purification folds. The resulting product was 25 kDa, determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting. Benzoate dioxygenase activity was found to be 6.54 U/mL and the optimal pH and temperature were 8.5 and 25°C, respectively. Maximum velocity (Vmax) and Michaelis constant (Km) were 7.36 U/mL and 5.58 µM, respectively. The end metabolite from the benzoate dioxygenase reaction was cyclohexane dione, which was determined by gas chromatography mass spectrometry (GC-MS).
  15. Singh R, Ramachandra SS, Dayakara JK
    Cutis, 2016 Dec;98(6):E1-E2.
    PMID: 28099544
    MeSH terms: Antitrichomonal Agents/adverse effects; Drug Eruptions/diagnosis; Drug Eruptions/etiology*; Drug Eruptions/pathology; Humans; Male; Middle Aged; Mouth Mucosa/drug effects*; Mouth Mucosa/pathology; Tinidazole/adverse effects*
  16. Zafira Madzin, Faradiella Mohd Kusin, Mohd Shakirin Md Zahar, Siti Nurjaliah Muhammad
    MyJurnal
    The contamination of water bodies from heavy metals, either from natural sources or
    anthropogenic sources, has become a major concern to the public. Industrial activities with improper
    water treatment, and then leach into the water body, have become contaminated and harmful to
    consume. Passive remediation is one of the treatments introduced to counter this problem as it is a low
    cost but effective technique. After being widely acknowledged and through research conducted, the
    most suitable remediation technique found is the permeable reactive barriers (PRBs). PRB is defined
    as an in situ permeable treatment zone filled with reactive materials, designed to intercept and
    remediate a contaminant plume under natural hydraulic gradients. There have been many findings
    made from PRB which can be used to remove contaminants such as heavy metal, chlorinated solvents,
    carbonates and aromatic hydrocarbons. The most crucial criteria in making a successful PRB is the
    reactive media used to remove contaminants. The current paper presents an overview of the PRB
    selective medias that have been used and also the unresolved issue on the long term performance of
    PRB. The overall methodology for the application of PRB at a given site is also discussed in this
    paper. This inexpensive but effective technique is crucial as a sustainable technology in order to treat
    the drainage before it enters water tables to prevent water pollution and can be used as an alternative
    raw water source.
    MeSH terms: Carbonates; Drainage; Hydrocarbons, Aromatic; Research; Solvents; Water; Water Pollution; Water Purification; Metals, Heavy; Groundwater
  17. Mohammed, Konto, Tukur, Salamatu M., Watanabe, Mahira, Abd-rani, Puteri A.m., Lau, Seng F., Shettima, Yasheruram M., et al.
    MyJurnal
    Changes in tick-vector densities and a resultant incidence of tick-borne diseases are
    caused mainly by human activities affecting the environmental ecosystem, especially in tropical
    countries. As one of the most important invertebrate arthropod vectors of disease transmission, ticks
    are susceptible to changes in their environment due to their sole dependence of all their life stages on
    prevailing environment. Upon completion of their lifecycle, ticks depend on the availability of hosts
    and other several factors related to their surroundings to survive. This review discusses the major
    factors that influence the prevalence and distribution of tick-borne diseases among domestic animals
    in Malaysia. It is highly imperative to understand the factors that lead to increase in tick-vector
    populations, infection intensity and hence the spatial distribution of ticks and tick-borne diseases in
    order to prevent their emergence and resurgence as well as to serve as a basis for effectivecontrol.
    MeSH terms: Animals; Animals, Domestic; Arthropod Vectors; Humans; Human Activities; Invertebrates; Malaysia; Ticks; Incidence; Prevalence; Tick-Borne Diseases; Ecosystem
  18. Liew, Pit Sze, Nurulfiza Mat Isa, Omar Abdul Rahman, Aini Ideris, Mohd HAIRBEJO
    MyJurnal
    Infectious bursal disease (IBD), also known as the Gumboro disease, has been a great
    concern for poultry industry worldwide. The first outbreak of IBD due to very virulent (vv) IBD virus
    (IBDV) infection in Malaysia was reported in 1991. The major economic impact of the disease is high
    mortality and poor performance. The virus causes immunosuppression where if the infected chicken
    recovered from the acute disease, they become more susceptible to infections of other pathogens and
    fail to respond to vaccines. Therefore, prevention is important and vaccination has become the
    principal control measure of IBDV infection in chickens. The conventional attenuated live and killed
    vaccines are the most commonly used vaccines. With the advancement of knowledge and technology,
    new generation of genetically-engineered vaccines like viral vector and immune complex vaccines
    have been commercialised. Moreover, hatchery vaccination is becoming a common practise, in
    addition to farm vaccination. Currently, the disease is considerably under controlled with the
    introduction of vaccination. However, occasional field outbreaks are still commonly reported. The
    demand for vaccines that could suit the field situation continues to exist. The endemicity of disease,
    presence of challenge in the farm and maternally derived antibody in chicks are affecting the choice
    vaccine as well as the vaccine development and vaccination strategies. In this review, advances made
    in various vaccines that have been commercialised or under development, and challenges that they
    face, are outlined. Furthermore, how the emergence of vvIBDV affect the progress of vaccine
    development and influence its vaccination strategy are discussed.
    MeSH terms: Acute Disease; Animals; Antigen-Antibody Complex; Chickens; Disease Outbreaks; Infectious bursal disease virus; Malaysia; Poultry; Vaccination; Vaccines; Birnaviridae Infections
  19. Alomari. Nashwan K., Badronnisa Yusuf, Thamer Ahmed Mohammed Ali, Abdul Halim Ghazali
    MyJurnal
    Branching channel flow refers to any side water withdrawals from rivers or main channels.
    Branching channels have wide application in many practical projects, such as irrigation and drainage
    network systems, water and waste water treatment plants, and many water resources projects. In the
    last decades, extensive theoretical and experimental investigations of the branching open channels
    have been carried out to understand the characteristics of this branching flow, varying from case
    studies to theoretical and experimental investigations. The objectives of this paper are to review and
    summarise the relevant literatures regarding branching channel flow. These literatures were reviewed
    based on flow characteristics, physical characteristics, and modeling of the branching flow.
    Investigations of the flow into branching channel show that the branching discharge depends on many
    interlinked parameters. It increases with the decreasing of the main channel flow velocity and Froude
    number at the upstream of the branch channel junction. Also it increases with the increasing of the
    branch channel bed slope. In subcritical flow, water depth in the branch channel is always lower than
    the main channel water depth. The flow diversion to the branch channel leads to an increase of water
    depth at the downstream of the main channel. From the review, it is important to highlight that most
    of the study concentrated on flow characteristics in a right angle branch channel with a rigid boundary.
    Investigations on different branching angles with movable bed have still to be explored.
    MeSH terms: Drainage; Paper; Physical Examination; Water; Water Purification; Aquaporins; Rivers; Waste Water; Water Resources
  20. Ajadi, Abdullateef, Sabri, M.Y., Dauda, Akeem Babatunde, Ina-Salwany M.Y., Hasliza, A.H.
    MyJurnal
    Aquaculture, especially shrimp production is the world`s fastest growing food production
    industry, due to increased demands for seafood. Conceivably, disease outbreak is the major setback
    which brings about high mortality and reduction or loss of production. The conventional use of
    antibiotics both prophylactically and therapeutically had recently not only been ineffective but
    incriminated, in drug residue which poise danger to consumption by humans. Hence, a better
    alternative was importantly required. The use of Immunoprophylaxis has potential of being a safety
    measure in the prevention of outbreak of diseases or spread of already established disease in aquatic
    invertebrates, where the shrimps and prawns belong. Immunoprophylaxis includes the prevention of
    disease by production of passive immunity. The major infectious agents that are responsible for high
    morbidity and high mortality in shrimp industry are viruses and bacteria. Others include fungi,
    parasites and protozoans but less threatening than the two former. Among the referenced groups of
    bacteria in shrimp disease, gram negative bacteria are more virulent. Vibrio, a member of this gram
    negative groups has been implicated to be the most causative agent of bacterial infections in shrimps.
    This review examined the components of the cell membrane of these bacteria that have been reported
    to confer immunity on the host. They include outer membrane proteins, lipopolysaccharide and
    peptidoglycan. These components could elicit immune response and confer protection.
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