Browse publications by year: 2017

  1. Razali A, Fullerton CJ, Turci F, Hallett JE, Jack RL, Royall CP
    Soft Matter, 2017 May 03;13(17):3230-3239.
    PMID: 28401216 DOI: 10.1039/c6sm02221a
    We consider the sedimentation of a colloidal gel under confinement in the direction of gravity. The confinement allows us to compare directly experiments and computer simulations, for the same system size in the vertical direction. The confinement also leads to qualitatively different behaviour compared to bulk systems: in large systems gelation suppresses sedimentation, but for small systems sedimentation is enhanced relative to non-gelling suspensions, although the rate of sedimentation is reduced when the strength of the attraction between the colloids is strong. We map interaction parameters between a model experimental system (observed in real space) and computer simulations. Remarkably, we find that when simulating the system using Brownian dynamics in which hydrodynamic interactions between the particles are neglected, we find that sedimentation occurs on the same timescale as the experiments. An analysis of local structure in the simulations showed similar behaviour to gelation in the absence of gravity.
  2. Phuah NH, Azmi MN, Awang K, Nagoor NH
    Mol Cells, 2017 Apr;40(4):291-298.
    PMID: 28401751 DOI: 10.14348/molcells.2017.2285
    MicroRNAs (miRNAs) are short non-coding RNAs that regulate genes posttranscriptionally. Past studies have reported that miR-210 is up-regulated in many cancers including cervical cancer, and plays a pleiotropic role in carcinogenesis. However, its role in regulating response towards anti-cancer agents has not been fully elucidated. We have previously reported that the natural compound 1'S-1'-acetoxychavicol acetate (ACA) is able to induce cytotoxicity in various cancer cells including cervical cancer cells. Hence, this study aims to investigate the mechanistic role of miR-210 in regulating response towards ACA in cervical cancer cells. In the present study, we found that ACA down-regulated miR-210 expression in cervical cancer cells, and suppression of miR-210 expression enhanced sensitivity towards ACA by inhibiting cell proliferation and promoting apoptosis. Western blot analysis showed increased expression of mothers against decapentaplegic homolog 4 (SMAD4), which was predicted as a target of miR-210 by target prediction programs, following treatment with ACA. Luciferase reporter assay confirmed that miR-210 binds to sequences in 3'UTR of SMAD4. Furthermore, decreased in SMAD4 protein expression was observed when miR-210 was overexpressed. Conversely, SMAD4 protein expression increased when miR-210 expression was suppressed. Lastly, we demonstrated that overexpression of SMAD4 augmented the anti-proliferative and apoptosis-inducing effects of ACA. Taken together, our results demonstrated that down-regulation of miR-210 conferred sensitivity towards ACA in cervical cancer cells by targeting SMAD4. These findings suggest that combination of miRNAs and natural compounds could provide new strategies in treating cervical cancer.
    MeSH terms: Antineoplastic Agents/pharmacology*; Benzyl Alcohols/pharmacology*; Uterine Cervical Neoplasms/metabolism*; Female; Humans; Down-Regulation; Up-Regulation; Gene Expression Regulation, Neoplastic; Apoptosis/drug effects; Drug Resistance, Neoplasm/drug effects*; 3' Untranslated Regions; MicroRNAs/antagonists & inhibitors; MicroRNAs/genetics; MicroRNAs/metabolism*; Cell Line, Tumor; Cell Proliferation/drug effects; Smad4 Protein/genetics; Smad4 Protein/metabolism*
  3. Geml J, Morgado LN, Semenova-Nelsen TA, Schilthuizen M
    New Phytol, 2017 Jul;215(1):454-468.
    PMID: 28401981 DOI: 10.1111/nph.14566
    The distribution patterns of tropical ectomycorrhizal (ECM) fungi along altitudinal gradients remain largely unknown. Furthermore, despite being an iconic site for biodiversity research, virtually nothing is known about the diversity and spatial patterns of fungi on Mt Kinabalu and neighbouring mountain ranges. We carried out deep DNA sequencing of soil samples collected between 425 and 4000 m above sea level to compare richness and community composition of ECM fungi among altitudinal forest types in Borneo. In addition, we tested whether the observed patterns are driven by habitat or by geometric effect of overlapping ranges of species (mid-domain effect). Community composition of ECM fungi was strongly correlated with elevation. In most genera, richness peaked in the mid-elevation montane forest zone, with the exception of tomentelloid fungi, which showed monotonal decrease in richness with increasing altitude. Richness in lower-mid- and mid-elevations was significantly greater than predicted under the mid-domain effect model. We provide the first insight into the composition of ECM fungal communities and their strong altitudinal turnover in Borneo. The high richness and restricted distribution of many ECM fungi in the montane forests suggest that mid-elevation peak richness is primarily driven by environmental characteristics of this habitat and not by the mid-domain effect.
    MeSH terms: Borneo; Environment; Sequence Analysis, DNA; Mycorrhizae/classification; Mycorrhizae/genetics; Mycorrhizae/physiology*; Biodiversity*
  4. Yavari S, Malakahmad A, Sapari NB, Yavari S
    Water Sci Technol, 2017 Apr;75(7-8):1684-1692.
    PMID: 28402310 DOI: 10.2166/wst.2017.043
    Phytoremediation is an environmentally friendly and sustainable alternative for treatment of nitrogen-enriched wastewaters. In this study, Ta-khian (Hopea odorata) and Lagos mahogany (Khaya ivorensis), two tropical timber plants, were investigated for their performances in treatment of urea manufacturing factory effluent with high nitrogen (N) content. Plant seedlings received four concentrations of N (190, 240, 290 and 340 mg/L N) in laboratory-scale constructed wetlands every 4 days for a duration of 8 weeks. The solution volumes supplied to each container, amount of N recovered by plants and plant growth characteristics were measured throughout the experiment. Results showed that Ta-khian plants were highly effective at reducing N concentration and volume of water. A maximum of 63.05% N recovery was obtained by Ta-khian plants grown in 290 mg/L N, which was assimilated in the chlorophyll molecule structure and shoot biomass. Significant positive correlations have been shown between N recovery percentages and plant growth parameters. Ta-Khian plants can be applied as suitable phytoremediators for mitigating N pollution in water sources.
    MeSH terms: Biodegradation, Environmental; Industrial Waste/analysis; Nigeria; Nitrogen/metabolism; Urea/chemistry*; Water Purification/instrumentation; Water Purification/methods*; Meliaceae/metabolism*; Dipterocarpaceae/metabolism*; Waste Water/chemistry
  5. Zahari Taha, Lum, Shirley, Mohd Azraai Mohd Razman
    Movement Health & Exercise, 2017;6(1):47-57.
    MyJurnal
    Hypertension, the abnormal elevation of blood pressure, is one of the chronic diseases that usually
    comes with no symptom and signal. Hypertension is diagnosed if the systolic blood pressure (SBP)
    over 140 mm Hg or diastolic blood pressure (DBP) is over 90 mmHg. The purpose of this paper is
    to review methods of early diagnosis of hypertension by monitoring the SBP, DBP, and heart rate
    (HR) non-invasively. Although accurate measurement of BP and HR of a person can be obtained
    invasively, the measuring probe needs to place under patient’s skin, which in turn would cause
    discomfort to the patients and not to mention the possibility of thrombosis to occur. External
    pressures are necessary to induce to the artery in order to measure BP and HR by using auscultatory
    and oscillometric methods, hence, a pressure cuff is used to measure BP. The pressure cuff will
    restrict the motion of the patient and it is rendered not suitable for continuous monitoring. On the
    other hand, pulse transit time (PTT) and photoplethysmography (PPG) methods are introduced to
    measure BP non-invasively without the use of a cuff. The limitation of PTT over PPG is PTT needs
    both PPG waveform and ECG waveform to estimate BP, and artificial phase lag might occur which
    will affect the reliability of the measured result. Therefore, for long-term hypertension monitoring,
    non-invasive mean using photoplethysmography method is preferred since it enables continuous
    monitoring without cuff and it requires only one waveform to estimate the BP as well as HR.
  6. Ahmed F. Farhan, Stephany, Maria J., Shamil K. Mahammed
    Movement Health & Exercise, 2017;6(1):39-45.
    MyJurnal
    In lower levels of play, the lateral ankle sprain is the most common injury, especially
    amongst male youth soccer players. Purpose: The aim of the present study is to evaluate the effects
    of an injury prevention program on the incidence of ankle injuries in male youth amateur players.
    Study Design: Randomised controlled trial study. Methods: Fifty boys (mean ±SD: age 13.3±0.4
    yr; body mass index of 20.9 ± 1.5 kg/m2
    ; stature: 1.6 ± 0.1 m) from two-sport schools, with 4.4 ±
    0.5 years playing experience, participated. Players were randomly assigned to either an
    experimental (EXP, n = 25) or a control (CON, n = 25) group. A physical exercise program
    designed exclusively for youth male soccer players to educate as well as increasing the awareness
    of the risk of injury of athletes and coaches. Over one year, all injuries were documented monthly
    by physiotherapists. Complete monthly injury reports were available for 50 players. Results: Nine
    ankle injuries occurred in the EXP group, and 20 injuries occurred in the CON group,
    corresponding to incidence rates of 0.96 and 2.16, respectively, per 1000 player hours, which
    equates to 55% fewer injuries in the EXP group. Conclusions: The incidence of ankle injuries
    among youth male soccer players can be reduced by the implementation of a multifaceted, soccerspecific
    physical exercise program. Coaches and players need better education regarding injury
    prevention strategies and should include such interventions as part of their regular training.
  7. Zahari Taha, Mohd Azraai Mohd Razman, Rabiu Muazu Musa, Faeiz Azizi Adnan, Anwar P.P. Abdul Majeed
    Movement Health & Exercise, 2017;6(1):31-38.
    MyJurnal
    The accurate transfer of information on the athletes’ performance in any sport is
    essential in enhancing the performance and overall coaching process. The provision of such
    information is favourable only if it is reliable. A cost-effective golf putting monitoring device
    namely the Putt.It.In was developed for analysing a golfers’ putting performance. Objectives: This
    study aims to investigate the reliability of the instrument in measuring the backswing distance, front
    swing distance, clubhead speed, ideal front swing distance and swing angle. Methods: A semiprofessional
    golfer (30 years of age ± 5.0 years’ experience) executed four strokes repeatedly from
    a distance of 2 m and 1 m using a Ram Zebra Mallet putter on a PGM golf mat. The intra-class
    correlation (ICC) coefficient is employed to test the reliability of the device whilst the
    Kolmogorov/Smirnov test was utilised to further reaffirm the reliability of the application in
    measuring the aforementioned parameters over test re-test between first two strokes of 2 m distance
    and the last two strokes of 1 m distance. Results: The ICC reveals 0.98 and 0.96 for both test 1 and
    2, as well as a Cronbach’s Alpha of 0.99 and 0.96, respectively suggesting excellent consistencies
    in the overall observations. Moreover, the Kolmogorov/Smirnov test re-test indicates that there is
    no significant difference between the first two 2 m strokes p > 0.05, and subsequent two 1 m strokes
    p > 0.05 highlighting its ability to recognise the pattern of the strokes applied in the four successive
    strokes. Conclusion: The Putt.It.In monitoring device is found to be reliable in measuring the
    backswing distance, front swing distance, clubhead speed, ideal front swing distance and swing
    angle. Professional and semi-professional golfers as well coaches could consider Putt.It.In device in
    monitoring strokes related parameters to enhance their performance due to its effectiveness in
    providing information on putting performance.
  8. Mohamad Razali Abdullah, Muhammad Syahrul Nidzam Akmal Hairi, Rabiu Muazu Musa, Ahmad Bisyri Husin Musawi Maliki, Norlaila Azura Kosni
    Movement Health & Exercise, 2017;6(1):21-29.
    MyJurnal
    Cardiovascular endurance is considered the most vital aspect of fitness due to its direct impact on
    human performance. For any uniform arm units to discharge their duties effectively, they need to
    meet up to the requirement of highest physical fitness level in respective of their body characteristics.
    This study aims to predict the relationship of physical characteristics and fitness variables on
    cardiovascular endurance performance among armed uniform package unit of Universiti Sultan
    Zainal Abidin. A total of 26 participants mean age and standard deviation of (±20.45 and 2.0)
    randomly selected from the three co-arm units of the university took part in this study. Standard
    physical characteristics measurement and fitness tests were conducted, and multiple linear regression
    was applied to predict their cardiovascular endurance performance as the dependent variables on their
    body characteristics and performance of physical fitness as the independent variable. A significant
    regression equation was obtained F (9, 16) = 4.97, p < 0.05, R2
    = 0.74 indicating that the model
    accounted for approximately 74% variability of the whole data set. Sensitivity prediction analysis of
    the contributions of each variable revealed that height, speed, agility, upper body strength and core
    body strength are factors to cardiovascular endurance p < 0.05 while, weight, waist circumference,
    neck circumference and flexibility were not predictors to cardiovascular endurance p > 0.05. To
    improve the cardiovascular performance of uniform arm units, certain physical and fitness variables
    have to be considered for an effective discharge of their duties.
  9. Rokiah Omar, Yau, Meng Kuan, Victor, Feizal Knight, Faudziah Abd Manan, Mohd Nizar Ahmad Padri
    Movement Health & Exercise, 2017;6(1):13-19.
    MyJurnal
    The ability to anticipate accurately and consistently plays a vital role to excel in sports
    performance. The present study was designed to determine the differences of visual anticipation
    time between athletes in open and closed skills sports. A total of 95 junior athletes, aged 13 to 16
    years old (Mean age=14.84±1.04 years) from Bukit Jalil Sports School participated in the study.
    Participants were distributed to open skills (n=47) and closed skills (n=48) sports according to
    their sports environment. Visual anticipation time was tested using Bassin Anticipation Timer in
    response to stimuli at the speed of 5, 10, and 15 mph, measuring absolute and variable error.
    Absolute error measured the accuracy of responses while variable error focused on the
    consistency of responses. The results of this investigation showed that in general open skills sport
    are not superior over anticipation ability in relations to accuracy and consistency as compared to
    closed skills sports across all speeds (p < 0.05). The evidence from this study suggested more
    emphasis on training related to eye and hand coordination would be imperative for open skills
    sports athletes to enhance in on-field sports performance. This study could serve as a base for
    future studies focusing on visual and hand coordination related to speed as anticipation has been
    proven to be the key leading to superior performance and for talent identification purposes. Future
    research concentrating on elite athletes as well as focused sports group would provide further
    insights on anticipation ability of athletes.
  10. Jawad AH, Azharul Islam M, Hameed BH
    Int J Biol Macromol, 2017 Feb;95:743-749.
    PMID: 27914966 DOI: 10.1016/j.ijbiomac.2016.11.087
    Fabrication of an immobilized cross-linked chitosan-epichlorohydrine thin film (CLCETF) onto glass plate for adsorption of reactive orange 16 (RO16) dye was successfully studied using the direct casting technique. Adsorption experiments were performed as a function of contact time, initial dye concentration (25mg/L to 350mg/L), and pH (3-11). The adsorption isotherm followed the Langmuir model. The adsorption capacity of CLECTF for RO16 was 356.50mg/g at 27±2°C. The kinetics closely followed the pseudo-second-order model. Results supported the potential use of an immobilized CLECTF as effective adsorbent for the treatment of reactive dye without using filtration process.
    MeSH terms: Adsorption; Azo Compounds/isolation & purification; Azo Compounds/chemistry*; Coloring Agents/isolation & purification; Coloring Agents/chemistry*; Epichlorohydrin/chemistry; Glass/chemistry*; Hydrogen-Ion Concentration; Kinetics; Water Pollutants, Chemical/isolation & purification; Water Pollutants, Chemical/chemistry*; Chitosan/chemistry*
  11. Huynh TT, Jamil I, Pianegonda NA, Blanksby SJ, Barker PJ, Manefield M, et al.
    Microbiologyopen, 2017 04;6(2).
    PMID: 27998037 DOI: 10.1002/mbo3.425
    Microbial colonization of prepainted steel, commonly used in roofing applications, impacts their aesthetics, durability, and functionality. Understanding the relevant organisms and the mechanisms by which colonization occurs would provide valuable information that can be subsequently used to design fouling prevention strategies. Here, next-generation sequencing and microbial community finger printing (T-RFLP) were used to study the community composition of microbes colonizing prepainted steel roofing materials at Burrawang, Australia and Kapar, Malaysia over a 52-week period. Community diversity was low and was dominated by Bacillus spp., cyanobacteria, actinobacteria, Cladosporium sp., Epicoccum nigrum, and Teratosphaeriaceae sp. Cultivation-based methods isolated approximately 20 different fungi and bacteria, some of which, such as E. nigrum and Cladosporium sp., were represented in the community sequence data. Fluorescence in situ hybridization imaging showed that fungi were the most dominant organisms present. Analysis of the sequence and T-RFLP data indicated that the microbial communities differed significantly between locations and changed significantly over time. The study demonstrates the utility of molecular ecology tools to identify and characterize microbial communities associated with the fouling of painted steel surfaces and ultimately can enable the targeted development of control strategies based on the dominant species responsible for fouling.
    MeSH terms: Base Sequence; Construction Materials/microbiology*; DNA, Bacterial/genetics; Paint/microbiology; Polymorphism, Restriction Fragment Length/genetics; Steel; Sequence Analysis, DNA; Biofilms/growth & development*; High-Throughput Nucleotide Sequencing
  12. Chan YC, Punzalan-Sotelo AM, Kannan TA, Shahrizaila N, Umapathi T, Goh EJH, et al.
    Muscle Nerve, 2017 Nov;56(5):919-924.
    PMID: 28093784 DOI: 10.1002/mus.25577
    INTRODUCTION: In this study we propose electrodiagnostic criteria for early reversible conduction failure (ERCF) in axonal Guillain-Barré syndrome (GBS) and apply them to a cohort of GBS patients.

    METHODS: Serial nerve conduction studies (NCS) were retrospectively analyzed in 82 GBS patients from 3 centers. The criteria for the presence of ERCF in a nerve were: (i) a 50% increase in amplitude of distal compound muscle action potentials or sensory nerve action potentials; or (ii) resolution of proximal motor conduction block with an accompanying decrease in distal latencies or compound muscle action potential duration or increase in conduction velocities.

    RESULTS: Of 82 patients from 3 centers, 37 (45%) had ERCF, 21 (26%) had a contrasting evolution pattern, and 8 (10%) had both. Sixteen patients did not show an amplitude increase of at least 50%.

    CONCLUSION: Our proposed criteria identified a group of patients with a characteristic evolution of NCS abnormality that is consistent with ERCF. Muscle Nerve 56: 919-924, 2017.

    MeSH terms: Autoantibodies/blood; Electrodiagnosis*; Female; Gangliosides/immunology; Humans; International Cooperation; Male; Neural Conduction/physiology*; Retrospective Studies; Muscle, Skeletal/physiopathology; Evoked Potentials, Motor/physiology*; Guillain-Barre Syndrome/blood; Guillain-Barre Syndrome/pathology; Guillain-Barre Syndrome/physiopathology*
  13. Apolinário-Hagen J, Vehreschild V, Alkoudmani RM
    JMIR Ment Health, 2017 Feb 23;4(1):e8.
    PMID: 28232298 DOI: 10.2196/mental.6375
    BACKGROUND: Despite the advanced development of evidence-based psychological treatment services, help-seeking persons with mental health problems often fail to receive appropriate professional help. Internet-delivered psychotherapy has thus been suggested as an efficient strategy to overcome barriers to access mental health care on a large scale. However, previous research indicated poor public acceptability as an issue for the dissemination of Internet-delivered therapies. Currently, little is known about the expectations and attitudes toward Internet-delivered therapies in the general population. This is especially the case for countries such as Germany where electronic mental health (e-mental health) treatment services are planned to be implemented in routine care.

    OBJECTIVE: This pilot study aimed to determine the expectations and attitudes toward Internet-based psychotherapy in the general population in Germany. Furthermore, it aimed to explore the associations between attitudes toward Internet-based therapies and perceived stress.

    METHODS: To assess public attitudes toward Internet-based psychotherapy, we conducted both Web-based and paper-and-pencil surveys using a self-developed 14-item questionnaire (Cronbach alpha=.89). Psychological distress was measured by employing a visual analogue scale (VAS) and the 20-item German version of the Perceived Stress Questionnaire (PSQ). In addition, we conducted explorative factor analysis (principal axis factor analysis with promax rotation). Spearman's rank correlations were used to determine the associations between attitudes toward Internet-based therapies and perceived stress.

    RESULTS: Descriptive analyses revealed that most respondents (N=1558; female: 78.95%, 1230/1558) indicated being not aware of the existence of Internet-delivered therapies (83.46%, 1141/1367). The average age was 32 years (standard deviation, SD 10.9; range 16-76). Through exploratory factor analysis, we identified 3 dimensions of public attitudes toward Internet-based therapies, which we labeled "usefulness or helpfulness," "relative advantage or comparability," and "accessibility or access to health care." Analyses revealed negative views about Internet-based therapies on most domains, such as perceived helpfulness. The study findings further indicated ambivalent attitudes: Although most respondents agreed to statements on expected improvements in health care (eg, expanded access), we observed low intentions to future use of Internet-delivered therapies in case of mental health problems.

    CONCLUSIONS: This pilot study showed deficient "e-awareness" and rather negative or ambivalent attitudes toward Internet-delivered therapies in the German-speaking general population. However, research targeting determinants of the large-scale adoption of Internet-based psychotherapy is still in its infancy. Thus, further research is required to explore the "black box" of public attitudes toward Internet-delivered therapies with representative samples, validated measures, and longitudinal survey designs.

  14. Eskandari F, Abdullah KL, Zainal NZ, Wong LP
    J Clin Nurs, 2017 Dec;26(23-24):4479-4488.
    PMID: 28233363 DOI: 10.1111/jocn.13778
    AIMS AND OBJECTIVES: To investigate the knowledge, attitude, intention and practice of nurses towards physical restraint and factors influencing these variables.

    BACKGROUND: A literature review showed a lack of studies focused on the intention of nurses regarding physical restraint throughout the world. Considering that very little research on physical restraint use has been carried out in Malaysia, assessment of nurses' knowledge, attitude, intention and practice is necessary before developing a minimising programme in hospitals.

    DESIGN: A cross-sectional study was used.

    METHODS: A questionnaire to assess the knowledge, attitude, intention and practice was completed by all nurses (n = 309) in twelve wards of a teaching hospital in Kuala Lumpur.

    RESULTS: Moderate knowledge and attitude with strong intention to use physical restraint were found among the nurses. Less than half of nurses considered alternatives to physical restraint and most of them did not understand the reasons for the physical restraint. Nurses' academic qualification, read any information source during past year and nurses' work unit showed a significant association with nurses' knowledge. Multiple linear regression analysis found knowledge, attitude and intention were significantly associated with nurses' practice to use physical restraint.

    CONCLUSION: This study showed some important misunderstandings of nurses about using physical restraint and strong intention regarding using physical restraint. Findings of this study serve as a supporting reason for importance of educating nurses about the use of physical restraint.

    RELEVANCE TO CLINICAL PRACTICE: Exploring the knowledge, attitude, intention and current practice of nurses towards physical restraint is important so that an effective strategy can be formulated to minimise the use of physical restraints in hospitals.

    MeSH terms: Adult; Attitude of Health Personnel*; Cross-Sectional Studies; Hospitals, Teaching; Humans; Health Knowledge, Attitudes, Practice*; Malaysia; Male; Nursing Staff, Hospital/education*; Nursing Staff, Hospital/psychology; Surveys and Questionnaires; Restraint, Physical/psychology*; Young Adult
  15. Ling WC, Liu J, Lau CW, Murugan DD, Mustafa MR, Huang Y
    Biochem Pharmacol, 2017 Jul 15;136:76-85.
    PMID: 28396195 DOI: 10.1016/j.bcp.2017.04.007
    Salvianolic acid B (Sal B) is one of the most abundant phenolic acids derived from the root of Danshen with potent anti-oxidative properties. The present study examined the vasoprotective effect of Sal B in hypertensive mice induced by angiotensin II (Ang II). Sal B (25mg/kg/day) was administered via oral gavage for 11days to Ang II (1.2mg/kg/day)-infused C57BL/6J mice (8-10weeks old). The vascular reactivity (both endothelium-dependent relaxations and contractions) in mouse arteries was examined by wire myography. The production of reactive oxygen species (ROS), protein level and localization of angiotensin AT1 receptors and the proteins involved in ROS formation were evaluated using dihydroethidium (DHE) fluorescence, lucigenin-enhanced chemiluminescence, immunohistochemistry and Western blotting, respectively. The changes of ROS generating proteins were also assessed in vitro in human umbilical vein endothelial cells (HUVECs) exposed to Ang II with and without co-treatment with Sal B (0.1-10nM). Oral administration of Sal B reversed the Ang II-induced elevation of arterial systolic blood pressure in mice, augmented the impaired endothelium-dependent relaxations and attenuated the exaggerated endothelium-dependent contractions in both aortas and renal arteries of Ang II-infused mice. In addition, Sal B treatment normalized the elevated levels of AT1 receptors, NADPH oxidase subunits (NOx-2 and NOx-4) and nitrotyrosine in arteries of Ang II-infused mice or in Ang II-treated HUVECs. In summary, the present study provided additional evidence demonstrating that Sal B treatment for 11days reverses the impaired endothelial function and with a marked inhibition of AT1 receptor-dependent vascular oxidative stress. This vasoprotective and anti-oxidative action of Sal B most likely contributes to the anti-hypertensive action of the plant-derived compound.
    MeSH terms: Angiotensin II/toxicity*; Animals; Benzofurans/pharmacology; Benzofurans/therapeutic use*; Dose-Response Relationship, Drug; Drugs, Chinese Herbal/pharmacology; Drugs, Chinese Herbal/therapeutic use*; Endothelium, Vascular/drug effects; Endothelium, Vascular/physiology*; Humans; Hypertension/chemically induced*; Hypertension/drug therapy*; Hypertension/physiopathology; Male; Mice, Inbred C57BL; Organ Culture Techniques; Treatment Outcome; Mice; Human Umbilical Vein Endothelial Cells/drug effects; Human Umbilical Vein Endothelial Cells/physiology
  16. Ahmad F, Shankar EM, Yong YK, Tan HY, Ahrenstorf G, Jacobs R, et al.
    Front Immunol, 2017;8:338.
    PMID: 28396665 DOI: 10.3389/fimmu.2017.00338
    The CD1d-restricted invariant natural killer T (iNKT) cells are implicated in innate immune responses against human immunodeficiency virus (HIV). However, the determinants of cellular dysfunction across the iNKT cells subsets are seldom defined in HIV disease. Herein, we provide evidence for the involvement of the negative checkpoint regulator (NCR) 2B4 in iNKT cell alteration in a well-defined cohort of HIV-seropositive anti-retroviral therapy (ART) naïve, ART-treated, and elite controllers (ECs). We report on exaggerated 2B4 expression on iNKT cells of HIV-infected treatment-naïve individuals. In sharp contrast to CD4(-)iNKT cells, 2B4 expression was significantly higher on CD4(+) iNKT cell subset. Notably, an increased level of 2B4 on iNKT cells was strongly correlated with parameters associated with HIV disease progression. Further, iNKT cells from ART-naïve individuals were defective in their ability to produce intracellular IFN-γ. Together, our results suggest that the levels of 2B4 expression and the downstream co-inhibitory signaling events may contribute to impaired iNKT cell responses.
    MeSH terms: HIV; HIV Seropositivity; Humans; Immunity, Innate; Killer Cells, Natural; Cohort Studies; CD4-Positive T-Lymphocytes; HIV Infections; Disease Progression; Antigens, CD1d; Natural Killer T-Cells
  17. Goo CL, Tan KB
    Case Rep Dent, 2017;2017:9373818.
    PMID: 28396807 DOI: 10.1155/2017/9373818
    This report describes the clinical and technical aspects in the oral rehabilitation of an edentulous patient with knife-edge ridge at the mandibular anterior edentulous region, using implant-retained overdentures. The application of computer-aided design and computer-aided manufacturing (CAD/CAM) in the fabrication of the overdenture framework simplifies the laboratory process of the implant prostheses. The Nobel Procera CAD/CAM System was utilised to produce a lightweight titanium overdenture bar with locator attachments. It is proposed that the digital workflow of CAD/CAM milled implant overdenture bar allows us to avoid numerous technical steps and possibility of casting errors involved in the conventional casting of such bars.
    MeSH terms: Dental Porcelain; Denture, Overlay; Humans; Mouth, Edentulous; Titanium; Metal Ceramic Alloys; Computer-Aided Design; Workflow
  18. Hani H, Allaudin ZN, Mohd-Lila MA, Sarsaifi K, Rasouli M, Tam YJ, et al.
    Xenotransplantation, 2017 05;24(3).
    PMID: 28397308 DOI: 10.1111/xen.12302
    BACKGROUND: Dead islets replaced with viable islets are a promising offer to restore normal insulin production to a person with diabetes. The main reason for establishing a new islet source for transplantation is the insufficiency of human donor pancreas while using xenogeneic islets perhaps assists this problem. The expression of PDX1 is essential for the pancreas expansion. In mature β-cells, PDX1 has several critical roles such as glucose sensing, insulin synthesis, and insulin secretion. In this study, we aimed to evaluate the expression of pancreatic duodenal homeobox-1 (PDX1) in treated caprine islets in culture and to assess the protective effects of antioxidant factors on the PDX1 gene in cultured caprine islets.

    MATERIALS AND METHODS: Purified islets were treated with serum-free, serum, IBMX, tocopherol, or IBMX and tocopherol media. Quantitative polymerase chain reaction and Western blotting were carried out to compare the expression levels of PDX1 in treated purified islets cultured with different media.

    RESULTS: Islets treated with IBMX/tocopherol exhibited the highest fold change in the relative expression of PDX1 on day 5 post-treatment (relative expression: 6.80±2.08), whereas serum-treated islets showed the lowest fold changes in PDX1 expression on day 5 post-treatment (0.67±0.36), as compared with the expression on day 1 post-treatment. Insulin production and viability tests of purified islets showed superiority of islet at supplemented serum-free media with IBMX/tocopherol compared to other cultures (53.875%±1.59%).

    CONCLUSIONS: Our results indicated that supplemented serum-free medium with tocopherol and IBMX enhances viability and PDX1 gene expression compared to serum-added and serum-free media.

    MeSH terms: Animals; Antioxidants/pharmacology; Cell Survival/drug effects; Cell Survival/genetics; Cell Survival/physiology; Culture Media; Genes, Homeobox; Goats/genetics*; Goats/physiology*; Insulin/biosynthesis; Islets of Langerhans/cytology; Islets of Langerhans/drug effects; Islets of Langerhans/physiology*; Male; 1-Methyl-3-isobutylxanthine/pharmacology; Trans-Activators/genetics*; Trans-Activators/physiology*; Gene Expression/drug effects; Culture Media, Serum-Free; Homeodomain Proteins/genetics*; Homeodomain Proteins/physiology*; Tocopherols/pharmacology; In Vitro Techniques
  19. Abdulrahman SA, Rampal L, Othman N, Ibrahim F, Kadir Shahar H, Radhakrishnan AP
    Asia Pac J Public Health, 2017 May;29(4):304-314.
    PMID: 28397533 DOI: 10.1177/1010539517700471
    Medication adherence remains a critical link between the prescribed ART regimen and treatment outcome. Several factors may influence adherence behavior. This cross-sectional study aimed to highlight socioeconomic predictors of adherence behavior among a cohort of 242 adult Malaysian patients receiving antiretroviral therapy in Hospital Sungai Buloh, Malaysia, where they were enrolled in a parent study (single-blinded randomized controlled trial) between January and December 2014. Statistical analysis of secondary data on adherence behavior and sociodemographic characteristics of the patients revealed mean age of 33.4 years and ranged from 18 to 64 years; 88.8% were males. A total of 224 (93%) patients who completed 6 months' adherence assessment were included in the model. Of these, 135 (60.3%) achieved optimal adherence. Multivariate binary logistic regression analysis revealed that patient's income and ethnicity were significant predictors of adherence behavior. This may be valuable for targeted programmatic interventions to further enhance successful treatment outcomes among the target population.
    MeSH terms: Adolescent; Adult; Cross-Sectional Studies; Female; Humans; Malaysia; Male; Middle Aged; Socioeconomic Factors; HIV Infections/drug therapy*; Logistic Models; Anti-HIV Agents/therapeutic use*; Medication Adherence/psychology*; Medication Adherence/statistics & numerical data; Young Adult
  20. Corrado R, Lacorata G, Palatella L, Santoleri R, Zambianchi E
    Sci Rep, 2017 04 11;7:46291.
    PMID: 28397797 DOI: 10.1038/srep46291
    The multi-scale and nonlinear nature of the ocean dynamics dramatically affects the spreading of matter, like pollutants, marine litter, etc., of physical and chemical seawater properties, and the biological connectivity inside and among different basins. Based on the Finite-Scale Lyapunov Exponent analysis of the largest available near-surface Lagrangian data set from the Global Drifter Program, our results show that, despite the large variety of flow features, relative dispersion can ultimately be described by a few parameters common to all ocean sub-basins, at least in terms of order of magnitude. This provides valuable information to undertake Lagrangian dispersion studies by means of models and/or of observational data. Moreover, our results show that the relative dispersion rates measured at submesoscale are significantly higher than for large-scale dynamics. Auxiliary analysis of high resolution GPS-tracked drifter hourly data as well as of the drogued/undrogued status of the buoys is provided in support of our conclusions. A possible application of our study, concerning reverse drifter motion and error growth analysis, is proposed relatively to the case of the missing Malaysia Airlines MH370 aircraft.
    MeSH terms: Aircraft; Biological Phenomena; Malaysia; Motion; Oceans and Seas; Seawater
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