Browse publications by year: 2019

  1. Parrett JM, Mann DJ, Chung AYC, Slade EM, Knell RJ
    Ecol Lett, 2019 Oct;22(10):1629-1637.
    PMID: 31353816 DOI: 10.1111/ele.13358
    The effect of sexual selection on species persistence remains unclear. The cost of bearing ornaments or armaments might increase extinction risk, but sexual selection can also enhance the spread of beneficial alleles and increase the removal of deleterious alleles, potentially reducing extinction risk. Here we investigate the effect of sexual selection on species persistence in a community of 34 species of dung beetles across a gradient of environmental disturbance ranging from old growth forest to oil palm plantation. Horns are sexually selected traits used in contests between males, and we find that both horn presence and relative size are strongly positively associated with species persistence and abundance in altered habitats. Testes mass, an indicator of post-copulatory selection, is, however, negatively linked with the abundance of species within the most disturbed habitats. This study represents the first evidence from a field system of a population-level benefit from pre-copulatory sexual selection.
    MeSH terms: Animals; Beetles/anatomy & histology*; Beetles/physiology; Borneo; Male; Phenotype; Sexual Behavior, Animal*; Ecosystem
  2. Abdul Aziz FA, Abd Razak MA, Ahmad NA, Awaluddin SM, Lodz NA, Sooryanarayana R, et al.
    Asia Pac J Public Health, 2019 11;31(8_suppl):73S-79S.
    PMID: 31353928 DOI: 10.1177/1010539519862161
    Various factors contribute to suicidal attempt. This study aims to determine the relationship between suicidal attempt and its associated factors among school-going adolescents in Malaysia. Data from the National Health and Morbidity 2017 survey were analyzed. This survey was implemented as a nationwide school-based survey targeting adolescents 13 to 17 years old. Descriptive and multiple logistic regression analysis was done using SPSS version 20. A total of 27 399 adolescents participated in this survey. The prevalence of suicidal attempt was 6.9% (95% confidence interval = 6.2-7.7). Multivariate analysis found that the odds of suicidal attempt among adolescent with depression is 4.3 (adjusted odds ratio = 4.3; 95% confidence interval = 3.9-4.8). Other significant factors are young adolescent, non-Malay ethnicities especially Indian, adolescent with parents living apart, and those without peer support and parental connectedness. A holistic approach for the planning of preventative strategies and public health policies should be made according to these risk factors.
    Study name: National Health and Morbidity Survey (NHMS-2017)
    MeSH terms: Adolescent; Depression; Health Policy; Humans; Malaysia; Surveys and Questionnaires; Risk Factors; Schools; Suicide, Attempted; Prevalence; Multivariate Analysis; Confidence Intervals; Logistic Models; Odds Ratio
  3. Mizrahi N, Gilon C, Atre I, Ogawa S, Parhar IS, Levavi-Sivan B
    PMID: 31354632 DOI: 10.3389/fendo.2019.00469
    Neurokinin B (NKB) and its cognate receptor (NK3R) are emerging as important components of the neuroendocrine regulation of reproduction. Unlike mammalian tac3, which encodes only one mature peptide (namely NKB), two mature peptides are predicted for each tac3 gene in fish and frogs. Therefore, it was designated as Neurokinin F (NKF). Hormone analogs with high and long-lasting biological activity are important tools for physiological and biological research; however, the availability of piscine-specific analogs is very limited. Therefore, we have developed specific NKB and NKF analogs based on the structure of the mammalian NKB analog-senktide. These analogs, specifically designed for longer half-lives by methylation of proteolysis sites, exhibited activity equal to those of the native NKB and NKF in short-term signal-transduction assays of tilapia NKB receptors. However, the analogs were found to be able to significantly increase the release of luteinizing hormone (LH), follicle stimulating hormone (FSH) and growth hormone (GH) in tilapia, as fast as 1 h after intraperitoneal (IP) injection. The impact of the analogs on LH and FSH secretion lasted longer compared to the effect of native peptides and salmon GnRH analog (sGnRHa). In addition, we harvested pituitaries 24 h post injection and measured LH, FSH and GH mRNA synthesis. Both analogs elevated mRNA levels of LH and GH, but only NKB analog increased FSH mRNA levels in the pituitary and all GnRH forms in the brain. NKB receptors were co-localized with all three types the GnRH neurons in tilapia brain in situ. We previously showed a direct effect of NKB at the pituitary level, and these new results suggest that the stronger impact of the NKB analog on GTH release is also due to an indirect effect through the activation of GnRH neurons. These results suggest that novel synthetic NKB analogs may serve as a tool for both research and agricultural purposes. Finally, the biological activity and regulatory role of NKB in tilapia brain and pituitary suggest that the NKB/NKBR system in fish is an important reproductive regulator in a similar way to the kisspeptin system in mammals.
    MeSH terms: Animals; Follicle Stimulating Hormone; Luteinizing Hormone; Gonadotropin-Releasing Hormone; Mammals; Methylation; Neurons; Peptide Fragments; Pituitary Gland; Pituitary Hormones, Anterior; RNA, Messenger; Salmon; Growth Hormone; Substance P; Neurokinin B; Tilapia; Receptors, Neurokinin-3; Kisspeptins; Proteolysis
  4. Mikhael EM, Hussain SA, Shawky N, Hassali MA
    BMJ Open Diabetes Res Care, 2019;7(1):e000658.
    PMID: 31354953 DOI: 10.1136/bmjdrc-2019-000658
    Background: Medication non-adherence is a common problem among patients with diabetes. Patient-reported medication adherence scales are the most commonly used method to assess patient medication adherence, but up to today there is no specific tool for assessing medication adherence among patients with diabetes in Arab countries. This study aimed to develop and validate a new tool for assessment of adherence to antidiabetic medications among Iraqi patients with diabetes.

    Methods: The Iraqi Anti-Diabetic Medication Adherence Scale (IADMAS) consists of eight items. The face and content validity of the IADMAS were established via an expert panel. For convergent validity, the IADMAS was compared with the Medication Adherence Questionnaire (MAQ). For concurrent validity, the IADMAS was compared with glycosylated hemoglobin. A total of 84 patients with types 2 diabetes were recruited from a diabetes center in Baghdad, Iraq. Test-retest reliability was measured by readministering the IADMAS to the same patients 4 weeks later.

    Results: Only 80 patients completed the study (response rate: 95%). Reliability analysis of the IADMAS showed a Cronbach's alpha value of 0.712, whereas that of the MAQ was 0.649. All items in the IADMAS showed no significant difference in the test-retest analysis, indicating that the IADMAS has stable reliability. There was no difference in the psychometric properties of the IADMAS and the MAQ. The sensitivity and specificity of the IADMAS were higher than that of the MAQ (100% vs 87.5% and 33.9% vs 29.7%, respectively).

    Conclusion: The IADMAS developed in this study is a reliable and valid instrument for assessing antidiabetic medication adherence among Iraqi patients.

    MeSH terms: Adult; Aged; Blood Glucose/analysis; Cross-Sectional Studies; Diabetes Mellitus/drug therapy*; Diabetes Mellitus/epidemiology; Female; Follow-Up Studies; Hemoglobin A, Glycosylated/analysis; Humans; Hypoglycemic Agents/therapeutic use*; Iraq/epidemiology; Male; Middle Aged; Pilot Projects; Prognosis; Psychometrics; Research Design; Retrospective Studies; ROC Curve; Reproducibility of Results*; Biomarkers/analysis*; Medication Adherence/statistics & numerical data*
  5. Diyana JNA, Lokman IH, Fazila SHN, Latiffah H, Ibitoye EB, Hazfalinda HN, et al.
    J Parasitol Res, 2019;2019:7903682.
    PMID: 31354983 DOI: 10.1155/2019/7903682
    This is a retrospective study on bovine fascioliasis infection on cattle in Peninsular Malaysia, spanning from January 2007 to December 2017. Cattle were diagnosed with Fasciola based on the fecal examination and the results were reported to the Veterinary Regional Laboratories in Peninsular Malaysia. These records were analysed for the occurrence of bovine fascioliasis within that 11-year period. Records of annual diagnostic cases from five major Veterinary Regional Laboratories were examined: Bukit Tengah, Pulau Pinang (north); Kuantan, Pahang (east); Johor Bahru, Johor (south); Sepang, Selangor (west); and Kota Bharu, Kelantan (east). A positive fascioliasis infestation was calculated based on a number of positively infected cattle with Fasciola from a number of cattle examined. A total of 1988 cattle were examined during this period and 35 (1.76%) cattle were reported to be positive for bovine fascioliasis. Parasite infection was the highest at Bukit Tengah region (5.55%) where 19 cases were positive from 342 reported cases while, at Kuantan (4.96%), 15 positive cases were reported, unlike Johor Bahru (0.09%), with only 1 positive case from 1136 cattle examined. Sepang and Kelantan had no positive cases. These results showed that bovine fascioliasis was generally prevalent in the northern and southeast parts of the Peninsular Malaysia; however, there was no significant relationship between the region and the occurrence of fascioliasis.
    MeSH terms: Animals; Cattle; Fasciola; Fascioliasis; Malaysia; Mite Infestations; Parasitic Diseases; Retrospective Studies; Prevalence
  6. Yakubu A, Azlan A, Loh SP, Md Noor S
    J Obes, 2019;2019:4929131.
    PMID: 31354987 DOI: 10.1155/2019/4929131
    This review article stresses the effective role of dietary fish fillet docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on overweight as a risk factor of cardiovascular disease (CVD) via platelet phospholipid modification. Several reports have demonstrated that saturated fat in overweight evokes systemic inflammation and more importantly predisposes it to cardiovascular disorder. Prospective studies have shown that saturated fat is directly proportional to the level of arachidonic acids (AA), precursor of thromboxane in the platelet phospholipid membrane as omega-6 fatty acid in overweight and obese people. Some literature has demonstrated that omega-3 fatty acid from fish fillet ameliorates inflammation, reduces proinflammatory cytokine, inhibits signaling pathway, and regulates the physical composition of inflammatory leukocytes and free radicals (ROS). Yellow stripe scad (YSS) is a local Malaysian fish that has been shown to contain a comparable level of EPA/DHA content as observed in salmon. This review article will focus on the dietary role of fish fillet that will balance the omega-6 fatty acid/omega-3 fatty acid ratio in platelet phospholipid from YSS to manage and prevent healthy overweight/obesity-related risk factor of CVD and to avoid the risk orthodox drug treatment.
    MeSH terms: Adult; Animals; Anti-Inflammatory Agents/pharmacology; Anti-Inflammatory Agents/therapeutic use*; Docosahexaenoic Acids/pharmacology; Docosahexaenoic Acids/therapeutic use; Female; Fish Oils/pharmacology*; Fishes/classification*; Humans; Hypercholesterolemia/diet therapy*; Hypercholesterolemia/epidemiology; Hypercholesterolemia/prevention & control; Inflammation/diet therapy*; Inflammation/epidemiology; Inflammation/prevention & control; Malaysia/epidemiology; Male; Phospholipids/physiology*; Prospective Studies; Species Specificity; Eicosapentaenoic Acid/analogs & derivatives*; Eicosapentaenoic Acid/pharmacology; Eicosapentaenoic Acid/therapeutic use; Fatty Acids, Omega-3/pharmacology; Fatty Acids, Omega-3/therapeutic use; Fatty Acids, Omega-6/pharmacology; Fatty Acids, Omega-6/therapeutic use
  7. Hassan R, Othman MNA, Harith MN, Md Sah ASR
    Scientifica (Cairo), 2019;2019:3430968.
    PMID: 31355044 DOI: 10.1155/2019/3430968
    Gracilaria red algae are notable for their economic importance as agrophytes, sold as salad vegetable, and used as the base for selected food and nonalcoholic beverages. A wild population of Gracilaria exists in coastal areas of Sarawak, Malaysian Borneo, but there is only limited knowledge on species diversity and its abundance leaving the untapped economic potential of this resource. This study was carried out to determine diversity of wild Gracilaria populations in Lawas, Santubong, and Asajaya, Sarawak, using the combination of morphological character examination and 5' region of the mitochondrial cytochrome c oxidase 1 (CO1-5P) gene analysis. Identification of the species using morphological characters revealed three species, namely, Gracilaria changii, G. blodgettii, and G. arcuata, had been collected from the sampling sites. However, based on 672 bp CO1-5P gene sequence analysis, all the three species were identified as G. blodgettii; besides, low genetic divergence values (0.17%-0.34%) were scored between samples in this study with the same species in GenBank. In the phylogenetic trees, all samples in this study group together with other G. blodgettii have high bootstrap values; thus, this species is monophyletic. This study implies that species identification of Gracilaria and other seagrass taxa which have a phenotypic plasticity problem should include the CO1-5P gene analysis as it is a reliable gene marker for species diversity assessment.
  8. El-Ishaq A, Alshawsh MA, Chik ZB
    PeerJ, 2019;7:e7254.
    PMID: 31355056 DOI: 10.7717/peerj.7254
    Asparagus africanus Lam. is a plant used traditionally for natal care. This study evaluates the oestrogenic activities of aqueous root extract and screens for possible bioactive phytochemicals. Oestrogenicity of A. africanus was evaluated in ovariectomised rats treated with 50, 200, and 800 mg/kgBW doses twice daily for three days. Ethinyl estradiol (EE)1 mg/kg was used as positive control, and hormonal analysis and gene expression were carried out. The findings demonstrated that the extract produced a dose-dependent increase in the oestrogen levels with a significant increase compared to untreated rats. Pre-treatment with oestrogen receptor antagonist (ORA) prior to A. africanus treatment reversed the trend. Gene expression analysis on rats treated with 200 mg/kgBW A. africanus showed significant (p 
  9. Jalani MA, Yuliati L, Lee SL, Lintang HO
    Beilstein J Nanotechnol, 2019;10:1368-1379.
    PMID: 31355105 DOI: 10.3762/bjnano.10.135
    We report that transparent mesostructured silica/gold nanocomposite materials with an interpore distance of 4.1 nm, as-synthesized from a templated sol-gel synthesis method using discotic trinuclear gold(I) pyrazolate complex, were successfully utilized for the fabrication of thin film mesoporous silica nanocomposites containing gold nanoparticles. The material exhibited a highly ordered hexagonal structure when subjected to a thermal hydrogen reduction treatment at 210 °C. In contrast, when the material was subjected to calcination as a heat treatment from 190 to 450 °C, the thin film nanocomposites showed an intense d100 X-ray diffraction peak. Moreover, gold nanoparticles inside the thin film nanocomposites were confirmed by the presence of the d111 diffraction peak at 2θ = 38.2°, a surface plasmon resonance peak between 500-580 nm, and the spherical shape observed in the transmission electron microscope images, as well as the visual change in color from pink to purple. Interestingly, by simply dipping the material into a reaction solution of 4-nitrophenol at room temperature, the highly ordered structure of the as-fabricated silica/gold nanoparticle thin film composite after thermal hydrogen reduction at 210 °C resulted in an improved catalytic activity for the reduction of 4-nitrophenol to 4-aminophenol compared to the material calcined at 250 °C. Such catalytic activity is due to the presence of gold nanoparticles of smaller size in the silicate channels of the highly ordered mesoporous film nanocomposites.
  10. Yeap KC, Sivagurunathan PD, Raman P, Khalid KHM
    GMS Ophthalmol Cases, 2019;9:Doc25.
    PMID: 31355123 DOI: 10.3205/oc000114
    Purpose: To report a rare case of non-tuberculous mycobacterial (NTM) choroiditis masquerading as choroidal tumour, where the initial diagnosis was masked by keratitis. Casedescription: A 57-year-old heroin chaser with a pre-existing left eye blindness due to past blunt trauma presented with diffuse bacterial keratitis on the same side. Systemic examination revealed multiple non-tender cervical lymphadenopathies. B-scan ultrasonography showed a hyperechoic choroidal mass with surrounding exudative retinal detachment, resembling a choroidal tumour. However, computed tomography (CT) and magnetic resonance imaging (MRI) scan findings were suggestive of inflammatory choroidal changes. Inflammatory markers were significantly raised and infective screening was positive for HIV and Hepatitis C. Tuberculosis workup was normal. In view of intractable pain, evisceration was done and his vitreous humour was sent for polymerase chain reaction (PCR). It was reported to be positive for Mycobacterium Fortuitum. Conclusion: NTM ocular infections are rare, challenging to diagnose, and potentially sight threatening. Early recognition and prompt treatment is life and vision saving.
    MeSH terms: Blindness; Choroid Neoplasms; Choroiditis; Heroin; Hepatitis C; Keratitis; Magnetic Resonance Imaging; Nontuberculous Mycobacteria; Pain, Intractable; Retinal Detachment; Tomography, X-Ray Computed; Tuberculosis; Ultrasonography; Vitreous Body; HIV Infections; Eye Infections; Polymerase Chain Reaction; Mycobacterium fortuitum; Lymphadenopathy
  11. Abdul Kadir A, Abdul Kadir A, Abd Hamid R, Mat Jais AM, Omar J, Sadagatullah AN, et al.
    Biomed Res Int, 2019;2019:6979585.
    PMID: 31355276 DOI: 10.1155/2019/6979585
    Objectives: The objective of the study is to evaluate the chondroprotective activity of Channa striatus (Channa) and glucosamine sulphate (glucosamine) on histomorphometric examinations, serum biomarker, and inflammatory mediators in experimental osteoarthritis (OA) rabbit model.

    Design: Anterior cruciate ligament transection (ACLT) was performed to induce OA in thirty-three male New Zealand white rabbits and were randomly divided into three groups: Channa, glucosamine, and control group. The control group received drinking water and the Channa and glucosamine groups were orally administered with 51.4 mg/kg of Channa extract and 77.5 mg/kg of glucosamine sulphate in drinking water, respectively, for eight weeks and then sacrificed. The articular cartilage was evaluated macroscopically and histologically using semiquantitative and quantitative methods. Serum cartilage oligomeric matric protein (COMP), cyclooxygenase 2 (COX-2) enzyme, and prostaglandin E2 (PGE2) were also determined.

    Results: Macroscopic analysis revealed that Channa group have a significantly lower severity grade of total macroscopic score compared to the control (p < 0.001) and glucosamine (p < 0.05) groups. Semiquantitative histology scoring showed that both Channa and glucosamine groups had lower severity grading of total histology score compared to the control group (p < 0.001). In comparison with the control, Channa group had lower histopathological changes in three compartments of the joint compared to glucosamine group which had lower histological scoring in two compartments only. The cartilage thickness, area, and roughness of both Channa (p < 0.05) and glucosamine (p < 0.05) groups were superior compared to the control group. However, the Channa group demonstrated significantly less cartilage roughness compared to the glucosamine group (p < 0.05). Serum COMP levels were lower in both Channa (p < 0.05) and glucosamine (p < 0.05) groups compared to the control group.

    Conclusion: Both oral administration of Channa extract and glucosamine exhibited chondroprotective action on an ACLT OA-induced rabbit model. However, Channa was superior to glucosamine in maintaining the structure of the cartilage.

    MeSH terms: Animals; Disease Models, Animal; Drug Evaluation, Preclinical; Fishes*; Male; Osteoarthritis/drug therapy; Osteoarthritis/metabolism; Osteoarthritis/pathology; Rabbits; Anterior Cruciate Ligament/metabolism; Anterior Cruciate Ligament/pathology
  12. Mohd Ali MR, Lih Huey L, Foo PC, Goay YX, Ismail AS, Mustaffa KMF, et al.
    Biomed Res Int, 2019;2019:9451791.
    PMID: 31355287 DOI: 10.1155/2019/9451791
    Melioidosis and leptospirosis, caused by two different bacteria, Burkholderia pseudomallei and Leptospira spp., are potentially fatal infections that share a very similar spectrum of clinical features and cause significant mortality and morbidity in humans and livestock. Early detection is important for better clinical consequences. To our knowledge, there is no diagnostic tool available to simultaneously detect and differentiate melioidosis and leptospirosis in humans and animals. In this study, we described a duplex TaqMan probe-based qPCR for the detection of B. pseudomallei and Leptospira spp. DNA. The performance of the assay was evaluated on 20 B. pseudomallei isolates, 23 Leptospira strains, and 39 other microorganisms, as well as two sets of serially diluted reference strains. The duplex qPCR assay was able to detect 0.02 pg (~ 4 copies) Leptospira spp. DNA and 0.2 pg (~ 25.6 copies) B. pseudomallei DNA. No undesired amplification was observed in other microorganisms. In conclusion, the duplex qPCR assay was sensitive and specific for the detection of B. pseudomallei & Leptospira spp. DNA and is suitable for further analytical and clinical evaluation.
    MeSH terms: Animals; DNA, Bacterial/genetics*; Humans; Hydrolysis; Leptospira/genetics*; Burkholderia pseudomallei/genetics*; Real-Time Polymerase Chain Reaction*
  13. Kumar S, Sharma D, Narasimhan B, Ramasamy K, Shah SAA, Lim SM, et al.
    BMC Chem, 2019 Dec;13(1):96.
    PMID: 31355369 DOI: 10.1186/s13065-019-0613-8
    Heterocyclic 1,3-diazine nucleus is a valuable pharmacophore in the field of medicinal chemistry and exhibit a wide spectrum of biological activities. PharmMapper, a robust online tool used for establishing the target proteins based on reverse pharmacophore mapping. PharmMapper study is carried out to explore the pharmacological activity of 1,3-diazine derivatives using reverse docking program. PharmMapper, an open web server was used to recognize for all the feasible target proteins for the developed compounds through reverse pharmacophore mapping. The results were analyzed via molecular docking with maestro v11.5 (Schrodinger 2018-1) using GTPase HRas as possible target. The molecular docking studies displayed the binding behavior of 1,3-diazine within GTP binding pocket. From the docking study compounds s3 and s14 showed better docked score with anticancer potency against cancer cell line (HCT116). Hence, the GTPase HRas may be the possible target of 1,3-diazine derivatives for their anticancer activity where the retrieved information may be quite useful for developing rational drug designing. Furthermore the selected 1,3-diazine compounds were evaluated for their in vitro anticancer activity against murine macrophages cell line. 1,3-Diazine compounds exhibited good selectivity of the compounds towards the human colorectal carcinoma cell line instead of the murine macrophages. The toxicity study of the most active compounds was also performed on non cancerous HEK-293 cell line.
    MeSH terms: Animals; Guanosine Triphosphate; Humans; Macrophages; Colorectal Neoplasms; Drug Design; Information Storage and Retrieval; GTP Phosphohydrolases; Mice; HEK293 Cells; Molecular Docking Simulation
  14. Teik CK, Basri NI, Abdul Karim AK, Azrai Abu M, Ahmad MF, Abdul Ghani NA, et al.
    Arch Iran Med, 2019 06 01;22(6):340-343.
    PMID: 31356101
    Cerebral arteriovenous malformation (AVM) is a rare entity with an estimated prevalence of 0.01-0.05% in the general population. We reviewed hospital obstetric records during 2010-2017 and reported a case series of six patients with cerebral AVM in pregnancy, of which five patients had successful pregnancy, and one maternal mortality.
    MeSH terms: Adult; Intracranial Arteriovenous Malformations/diagnosis*; Intracranial Arteriovenous Malformations/surgery; Cerebral Hemorrhage/diagnosis; Cerebral Hemorrhage/surgery; Female; Humans; Infant, Newborn; Maternal Mortality; Pregnancy; Pregnancy Outcome
  15. Majeed Alneamy JS, A Hameed Alnaish Z, Mohd Hashim SZ, Hamed Alnaish RA
    Comput Biol Med, 2019 09;112:103348.
    PMID: 31356992 DOI: 10.1016/j.compbiomed.2019.103348
    Accurate medical disease diagnosis is considered to be an important classification problem. The main goal of the classification process is to determine the class to which a certain pattern belongs. In this article, a new classification technique based on a combination of The Teaching Learning-Based Optimization (TLBO) algorithm and Fuzzy Wavelet Neural Network (FWNN) with Functional Link Neural Network (FLNN) is proposed. In addition, the TLBO algorithm is utilized for training the new hybrid Functional Fuzzy Wavelet Neural Network (FFWNN) and optimizing the learning parameters, which are weights, dilation and translation. To evaluate the performance of the proposed method, five standard medical datasets were used: Breast Cancer, Heart Disease, Hepatitis, Pima-Indian diabetes and Appendicitis. The efficiency of the proposed method is evaluated using 5-fold cross-validation and 10-fold cross-validation in terms of mean square error (MSE), classification accuracy, running time, sensitivity, specificity and kappa. The experimental results show that the efficiency of the proposed method for the medical classification problems is 98.309%, 91.1%, 91.39%, 88.67% and 93.51% for the Breast Cancer, Heart Disease, Hepatitis, Pima-Indian diabetes and Appendicitis datasets, respectively, in terms of accuracy after 30 runs for each dataset with low computational complexity. In addition, it has been observed that the proposed method has efficient performance compared with the performance of other methods found in the related previous studies.
    MeSH terms: Algorithms*; Diagnosis, Computer-Assisted*; Education, Medical*; Humans; Neural Networks (Computer)*; Fuzzy Logic*
  16. Shahar FS, Hameed Sultan MT, Lee SH, Jawaid M, Md Shah AU, Safri SNA, et al.
    J Mech Behav Biomed Mater, 2019 11;99:169-185.
    PMID: 31357064 DOI: 10.1016/j.jmbbm.2019.07.020
    Since ancient Egypt, orthosis was generally made from wood and then later replaced with metal and leather which are either heavy, bulky, or thick decreasing comfort among the wearers. After the age of revolution, the manufacturing of products using plastics and carbon composites started to spread due to its low cost and form-fitting feature whereas carbon composite were due to its high strength/stiffness to weight ratio. Both plastic and carbon composite has been widely applied into medical devices such as the orthosis and prosthesis. However, carbon composite is also quite expensive, making it the less likely material to be used as an Ankle-Foot Orthosis (AFO) material whereas plastics has low strength. Kenaf composite has a high potential in replacing all the current materials due to its flexibility in controlling the strength to weight ratio properties, cost-effectiveness, abundance of raw materials, and biocompatibility. The aim of this review paper is to discuss on the possibility of using kenaf composite as an alternative material to fabricate orthotics and prosthetics. The discussion will be on the development of orthosis since ancient Egypt until current era, the existing AFO materials, the problems caused by these materials, and the possibility of using a Kenaf fiber composite as a replacement of the current materials. The results show that Kenaf composite has the potential to be used for fabricating an AFO due to its tensile strength which is almost similar to polypropylene's (PP) tensile strength, and the cheap raw material compared to other type of materials.
    MeSH terms: Animals; Ankle; Biocompatible Materials/chemistry; Carbon/chemistry; Foot; Humans; Materials Testing; Plastics/chemistry; Polypropylenes/chemistry*; Prosthesis Design*; Surface Properties; Temperature; Tensile Strength; Hibiscus; Shear Strength; History, 20th Century; History, 21st Century; History, Ancient; Foot Orthoses/history*; Foot Orthoses/trends*; Printing, Three-Dimensional
  17. Cheong WL, Mohan D, Warren N, Reidpath DD
    Mult Scler Relat Disord, 2019 Oct;35:86-91.
    PMID: 31357123 DOI: 10.1016/j.msard.2019.07.009
    BACKGROUND: Despite the global consensus on the importance of palliative care for patients with multiple sclerosis (MS), many patients in developing countries do not receive palliative care. Improving access to palliative care for MS requires a contextual understanding of how palliative care is perceived by patients and health professionals, the existing care pathways, and barriers to the provision of palliative care.

    OBJECTIVE: This study aims to examine and contrast the perceptions of MS patients, neurologists, and palliative care physicians towards providing palliative care for patients with MS in Malaysia.

    METHODS: 12 MS patients, 5 neurologists, and 5 palliative care physicians participated in this qualitative study. Each participant took part in a semi-structured interview. The interviews were transcribed verbatim, and analysed using an iterative thematic analysis approach.

    RESULTS: Patients and neurologists mostly associated palliative care with the end-of-life and struggled to understand the need for palliative care in MS. Another barrier was the lack of understanding about the palliative care needs of MS patients. Palliative care physicians also identified the scarcity of resources and their lack of experience with MS as barriers. The current referral-based care pathway itself was found to be a barrier to the provision of palliative care.

    CONCLUSIONS: MS patients in Malaysia face several barriers in accessing palliative care. Overcoming these barriers will require improving the shared understanding of palliative care and its role in MS. The existing care pathway also needs to be reformed to ensure that it improves access to palliative care for MS patients.

    MeSH terms: Adult; Attitude of Health Personnel*; Health Services Accessibility*; Humans; Health Knowledge, Attitudes, Practice*; Malaysia; Multiple Sclerosis/therapy*; Palliative Care*; Physicians*; Qualitative Research; Neurologists
  18. Islam R, Nazifa TH, Yuniarto A, Shanawaz Uddin ASM, Salmiati S, Shahid S
    Waste Manag, 2019 Jul 15;95:10-21.
    PMID: 31351595 DOI: 10.1016/j.wasman.2019.05.049
    Associated with the continuing increase of construction activities such as infrastructure projects, commercial buildings and housing programs, Bangladesh has been experiencing a rapid increase of construction and demolition (C&D) waste. Till now, the generation rate of C&D waste has not been well understood or not explicitly documented in Bangladesh. This study aims to provide an approach to estimate C&D waste generation using waste generation rates (WGR) through regression analysis. Furthermore, analyses the economic benefit of recycling C&D waste. The results revealed that WGR 63.74 kg/m2 and 1615 kg/m2 for construction and demolition activities respectively. Approximately, in financial year (FY) 2016, 1.28 million tons (0.149 construction and 1.139 demolition) waste were generated in Dhaka city, of which the three largest proportions were concrete (60%), brick/block (21%) and mortar (9%). After collection they were dumped in either landfills or unauthorized places. Therefore, it can be summarized as: waste is a resource in wrong place. The results of this study indicate that rapid urbanization of Dhaka city would likely experience the peak in the generation of C&D waste. This paper thus designates that C&D waste recycling is an entrepreneurial activity worth venturing into and an opportunity for extracting economic and environmental benefits from waste. The research findings also show that recycling of concrete and brick waste can add economic value of around 44.96 million USD. In addition, recycling of C&D waste leads to important reductions in CO2 emissions, energy use, natural resources and illegal landfills. Therefore, the findings of WGR and economic values provide valuable quantitative information for the future C&D waste management exercises of various stakeholders such as government, industry and academy.
    MeSH terms: Bangladesh; Construction Materials; Industrial Waste; Waste Management*; Recycling; Construction Industry*; Waste Disposal Facilities
  19. Karim KMR, Husaini A, Sing NN, Tasnim T, Mohd Sinang F, Hussain H, et al.
    Protein Expr Purif, 2019 12;164:105462.
    PMID: 31351992 DOI: 10.1016/j.pep.2019.105462
    The Aspergillus flavus NSH9 gene, encoding a pH and thermostable glucoamylase with a starch binding domain (SBD), was expressed in Pichia pastoris to produce recombinant glucoamylase (rGA2). The full-length glucoamylase gene (2039 bp), and cDNA (1839 bp) encode a 612 amino acid protein most similar to glucoamylase from Aspergillus oryzae RIB40; the first 19 amino acids are presumed to be a signal peptide for secretion, and the SBD is at the C-terminal. The cDNA was successfully secreted by Pichia at 8.23 U mL-1, and the rGA2 was found to be: a 80 kDa monomer, stable from pH 3.0-9.0, with optimum catalytic activity at pH 5.0, active at temperatures up to 80°C (rGA2 retained 58% of its activity after 60 min of incubation at 70°C), and metal ions such as Na+, K+, Ca++ and Mg++ enhanced rGA2 enzyme activity. The starch degrading ability of rGA2 was also observed on raw sago starch and where prolonged incubation generated larger, deeper, holes on the starch granules, indicating rGA2 is an excellent candidate for industrial starch processing applications.
    MeSH terms: Amino Acid Sequence; Aspergillus flavus/enzymology*; Aspergillus flavus/genetics; Aspergillus flavus/metabolism; Aspergillus flavus/chemistry; Cloning, Molecular/methods; Glucan 1,4-alpha-Glucosidase/genetics; Glucan 1,4-alpha-Glucosidase/metabolism*; Glucan 1,4-alpha-Glucosidase/chemistry; Hydrogen-Ion Concentration; Phylogeny; Pichia/genetics; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Recombinant Proteins/chemistry; Starch/metabolism*; Transformation, Genetic; Sequence Alignment
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