Browse publications by year: 2019

  1. Huda-Shakirah AR, Kee YJ, Hafifi ABM, Mohamad Azni NN, Zakaria L, Mohd MH
    Mycobiology, 2019;47(4):408-414.
    PMID: 32010462 DOI: 10.1080/12298093.2019.1682448
    Crinum asiaticum and Hymenocallis littoralis, commonly known as spider lilies are bulbous perennial and herbaceous plants that widely planted in Malaysia as ornamental. During 2015-2016, symptom of leaf blight was noticed on the hosts from several locations in Penang. The symptom appeared as irregular brown to reddish lesions surrounded by yellow halos. As the disease progressed, the infected leaves became blighted, dried, and fell off with the presence of black microsclerotia and pycnidia on the lesions parts. The present study was conducted to investigate the causal pathogen of leaf blight on C. asiaticum and H. littoralis. Based on morphological characteristics and DNA sequences of internal transcribed spacer (ITS) region and translation elongation factor 1-alpha (TEF1-α) gene, the causal pathogen was identified as Macrophomina phaseolina. Phylogenetic analysis of combined dataset of ITS and TEF1-α grouped the isolates studied with other isolates of M. phaseolina from GenBank. The grouping of the isolates was supported by 96% bootstrap value. Pathogenicity test proved the role of the fungus in causing leaf blight on both hosts.
  2. Jafri MA, Kalamegam G, Abbas M, Al-Kaff M, Ahmed F, Bakhashab S, et al.
    Front Cell Dev Biol, 2019;7:380.
    PMID: 32010693 DOI: 10.3389/fcell.2019.00380
    Osteoarthritis (OA) is a chronic degenerative joint disorder associated with degradation and decreased production of the extracellular matrix, eventually leading to cartilage destruction. Limited chondrocyte turnover, structural damage, and prevailing inflammatory milieu prevent efficient cartilage repair and restoration of joint function. In the present study, we evaluated the role of secreted cytokines, chemokines, and growth factors present in the culture supernatant obtained from an ex vivo osteochondral model of cartilage differentiation using cartilage pellets (CP), bone marrow stem cells (BM-MSCs), and/or BM-MSCs + CP. Multiplex cytokine analysis showed differential secretion of growth factors (G-CSF, GM-CSF, HGF, EGF, VEGF); chemokines (MCP-1, MIP1α, MIP1β, RANTES, Eotaxin, IP-10), pro-inflammatory cytokines (IL-1β, IL-2, IL-5, IL-6, IL-8, TNFα, IL-12, IL-15, IL-17) and anti-inflammatory cytokines (IL-4, IL-10, and IL-13) in the experimental groups compared to the control. In silico analyses of the role of stem cells and CP in relation to the expression of various molecules, canonical pathways and hierarchical cluster patterns were deduced using the Ingenuity Pathway Analysis (IPA) software (Qiagen, United States). The interactions of the cytokines, chemokines, and growth factors that are involved in the cartilage differentiation showed that stem cells, when used together with CP, bring about a favorable cell signaling that supports cartilage differentiation and additionally helps to attenuate inflammatory cytokines and further downstream disease-associated pro-inflammatory pathways. Hence, the autologous or allogeneic stem cells and local cartilage tissues may be used for efficient cartilage differentiation and the management of OA.
    MeSH terms: Epidermal Growth Factor; Interleukin-2; Tumor Necrosis Factor-alpha; United States; Interleukin-4; Interleukin-5; Interleukin-6; Granulocyte-Macrophage Colony-Stimulating Factor; Cytokines; Interleukin-10; Interleukin-13; Chemokine CCL5; Chemokine CXCL10
  3. Nor Hashimah AMM, Sakthiswary R, Shaharir SS, Wahab A
    Arch Rheumatol, 2019 Dec;34(4):387-394.
    PMID: 32010887 DOI: 10.5606/ArchRheumatol.2019.7243
    Objectives: This study aims to compare the serum pyridinoline (Pyd) levels between rheumatoid arthritis (RA) patients and healthy controls and to determine the correlation of serum Pyd levels with radiographic joint erosions.

    Patients and methods: Serum samples were obtained from 48 patients with RA (9 males, 39 females; mean age 60.5 years; range 54 to 64 years) and 48 healthy controls (9 males, 39 females; mean age 57.5 years; range, 47 to 65 years). The enzyme-linked immunosorbent assay method was used for quantitative analysis of serum Pyd. Besides, all RA patients were assessed for joint damage based on modified Sharp score, disease activity based on disease activity score in 28 joints and functional capacity based on health assessment questionnaire-disability index.

    Results: The median serum Pyd levels were significantly higher among the RA patients (110.20 ng/mL [92.30-120.64]) compared to the controls (98.22 ng/mL [85.54-111.41]); p<0.05. RA patients with erosive disease had significantly higher serum Pyd levels (p=0.024). There was a significant positive correlation between serum Pyd levels and joint erosion score (r=0.285, p=0.049). The serum Pyd levels had no demonstrable association with disease activity or functional capacity. Steroid therapy did not appear to influence the levels of serum Pyd.

    Conclusion: Rheumatoid arthritis patients had significantly higher levels of serum Pyd compared to healthy controls. The serum Pyd levels had significant correlation with radiographic joint erosions which reflected disease damage.

  4. Shchukin D, Lisova G, Khareba G, Polyakov M, Antonyan I, Kopytsya M, et al.
    PMID: 32011290
    In most cases RCC with venous extension is an indication for radical nephrectomy. However, in patients with imperative indications for nephron-sparing surgery a kidney resection with thrombectomy in rare situations is possible. We present a surgical technique for nephron-sparing surgery in patients with tumor spreading into the main renal vein. From 2007 to 2019 seven partial nephrectomies with removal of the tumor thrombus from the main renal vein were performed. Absolute imperative indications were recorded in 6 (85.7%) patients (4 with solitary kidney, 2 with bilateral tumors). Dimensions of renal tumor averaged 6.5±2.3 cm. Intravenous part of the tumor penetrated the initial portion of the renal vein in 3 cases, main trunk of the renal vein in 3 cases, and cavarenal portion of the IVC in 1 case. The average length of the tumor thrombus was 24.0±6.9 mm, and the width was 9.3±4.8 mm. Tumor thrombi were classified as intrarenal and extrarenal. In accordance with this principle surgical procedures were divided into partial nephrectomy with intrarenal (3/42.9%) and extrarenal thrombectomy (4/57.1%). Intrarenal thrombectomy was characterized by thrombus removal out of the vein lumen from the side of the kidney resection area, while extrarenal thrombectomy included additional opening the lumen of the main renal vein or inferior vena cava (IVC). In one patient hypothermic perfusion of the kidney in situ was used. In two cases the extracorporeal partial nephrectomy with kidney autotransplantation was performed. Warm ischemia time averaged 14.0 minutes. The time of ischemia during hypothermic perfusion in situ reached 76 minutes, the time of cold ischemia during extracorporeal surgery averaged 68.0 minutes. The volume of blood loss did not exceed an average of 621.4±146.8 ml. Early postoperative complications occurred in 4 (57.1%) cases (bleeding -1, ischemic stroke -1, deterioration of renal failure -2). Postoperative mortality was registered in 1 patient due to bleeding and heart failure. The follow-up period averaged 28.7±18.9 months. Distant metastases occurred in 1 (14.3%) patient after 16 months. Local tumor recurrence in the remnant kidney after nephron-sparing surgery was not detected in any case. Nephron-sparing surgery may be used to treat patients with kidney tumors and neoplastic venous thrombosis. However, this complex surgical approach requires further technical improvement.
    MeSH terms: Humans; Nephrectomy; Renal Veins; Retrospective Studies; Vena Cava, Inferior; Thrombectomy*; Organ Sparing Treatments
  5. Rayanakorn A, Katip W, Goh BH, Oberdorfer P, Lee LH
    Infect Drug Resist, 2019;12:3955-3965.
    PMID: 32021313 DOI: 10.2147/IDR.S233326
    Purpose: Streptococcus suis (S. suis) is an emerging zoonotic disease mainly in pigs, causing serious infections in humans with high prevalence in Southeast Asia. Despite a relatively high mortality rate, there are limited data regarding the risk factors of this life-threatening infection. Therefore, a 13-year retrospective cohort study in Chiang Mai, Thailand during 2005-2018 was conducted to explore risk factors associated with S. suis mortality and to update the outcomes of the disease.

    Patients and methods: S. suis positive cases were derived from those with positive S. suis isolates from microbiological culture results and Matrix-Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF). Potential risk factors of mortality were identified using univariate and multivariate logistic regression.

    Results: Of 133 patients with culture-proven S. suis infection identified, there were 92 males and 41 females. The mean age was 56.47 years. Septicemia (55.64%) was the most common clinical manifestation followed by meningitis (37.59%) and infective endocarditis (25.56%). Alcohol drinking and raw pork consumption were documented in 66 (49.62%) and 49 (36.84%) cases respectively. The overall mortality rate was 12.03% (n=16). According to the multivariate analysis, the independent risk factors for mortality were prolonged bacteremia ≥ 6 days (OR = 43.57, 95% CI = 2.46-772.80, P =0.010), septic shock (OR = 13.34, 95% CI = 1.63-109.03, P =0.016), and direct bilirubin > 1.5 mg/dL (OR = 12.86, 95% CI = 1.91-86.59, P =0.009).

    Conclusion: S. suis is not infrequent in Northern Thailand, where the cultural food habit of raw pork eating is still practiced. To the best of our knowledge, this is the largest series focusing on risk factors of S. suis mortality which has been conducted in Thailand. Prolonged bacteremia ≥ 6 days, septic shock, and direct bilirubin > 1.5 mg/dL were strong predictors associated with S. suis mortality. The mortality risk factors identified may be further utilized in clinical practice and future research to improve patient outcomes.

    MeSH terms: Red Meat; Animals; Endocarditis; Endocarditis, Bacterial; Female; Humans; Male; Retrospective Studies; Risk Factors; Shock, Septic; Swine; Thailand; Zoonoses; Prevalence; Multivariate Analysis; Logistic Models; Bacteremia; Streptococcus suis; Sepsis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  6. Muhammad Zamir Mohyedin, Afiq Radzwan, Mohammad Fariz Mohamad Taib, Rosnah Zakaria, Nor Kartini Jaafar, Masnawi Mustaffa, et al.
    Scientific Research Journal, 2019;16(2):77-88.
    MyJurnal
    Bi2Se3 is one of the promising materials in thermoelectric devices and is environmentally friendly due to its efficiency to perform in room temperature. Structural and electronic properties of Bi2Se3 were investigated based on the first-principles calculation of density functional theory (DFT) using CASTEP computer code. The calculation is conducted within the exchange-correlation of local density approximation (LDA) and generalised gradient approximation within the revision of Perdew-Burke-Ernzerhof (GGA-PBE) functional. A comparative study is carried out between the electronic properties of LDA and GGA-PBE. Lattice parameter and band gap are consistent with the other reports. Calculation from LDA is more accurate and has a better agreement than GGA-PBE in describing the lattice parameter of Bi2Se3. Band gap and density of states of LDA show higher electrical conductivity than GGA-PBE. Both LDA and GGA-PBE have same degree of thermal conductivity due to the occurrence of indirect band gap at same range of wave vector.
  7. Normah Ismail, Wan Najma Farhaten Wan A Latiff
    Scientific Research Journal, 2019;16(2):59-76.
    MyJurnal
    This study was conducted to evaluate the effect of pre-treatment on the fishy flavour and odour removal of gelatine extracted from the skin of sutchi catfish (Pangasius sutchi). Pre-treatment of the skin involved soaking at 4°C in distilled water (GC), lime followed by tamarind (GLT) or salt followed by activated carbon (GSC) prior to extraction in warm distilled water (50°C) for 12 hours. Yield, physical properties and sensory were determined. Results showed that GLT produced highest yield (19.72%) compared to GSC (15.01%) and GC (15.81%). Although, GLT exhibited lowest gel strength (282.29g), viscoelasticity (14.1ºC) and setting point (10.46ºC) compared to other pre-treatments, fishy flavour and odour of the gelatine were almost absent with the score of 1.68 and 1.74, respectively. These values were below those of reference which are 1.87 (fishy flavour) and 2.71 (fishy odour) denoting from ‘absent to weak’. Since fishy flavour and odour were almost absent, soaking sutchi catfish skin in lime followed by tamarind could be a good method for achieving the desired sensory attributes of the freshwater fish by the reduction of the gelatine off flavour.
  8. Muhammad Mustakim Mohd Ghaztar, Nik Noor Idayu Nik Ibrahim, Sarani Zakaria, Ahmad Zafir Romli
    Scientific Research Journal, 2019;16(2):43-58.
    MyJurnal
    Natural fibre is known useful in many applications however, the existing fibre treatment method able to reduce its overall properties. In this study, a new, simple and cost-effective fibre coating treatment method was developed which was able to improve the physical and morphological properties that open a new path for natural based materials to be used in a more robust application. The physical and morphological properties of various coated kenaf fibres were analysed to comprehend the cutting behaviour of coated fibres after subjected to the pulverisation process. The kenaf fibres were individually immersed in 1:4, 1:5 and 1:6 epoxy to acetone coating solutions prior cured, and pulverised consecutively using 5 mm, 1 mm, 0.5 mm and 0.25 mm mesh sizes aperture. The morphological characteristic was analysed using polarised optical and scanning electron microscope. The result showed that 1:6 coating ratio solution able to effectively coat the fibres’ aspect ratio that forming individual coated fibre which in long length pulverised fibres. Moreover, the low viscous 1:6 solution able to penetrate inside fibre structure that supported by density and fibre crosssection analysis compare to the other solutions. In future, this analysis is crucial to give insight on the coated fibres behaviour after subjected to the mechanical means of cutting process that later relates to the reinforcing mechanism in the composite samples.
  9. Ahmad Aizuddin Ariffin, Nik Noor Idayu Nik Ibrahim, Solehuddin Shuib, Ahmad Zafir Romli, Nur Faiqa Ismail
    Scientific Research Journal, 2019;16(2):27-42.
    MyJurnal
    Surface topography and morphological behaviours are the important aspects in the application of surface bearing as it deals with the contact area of objects upon motion. Improved surface bearing will be set as an indicator for tribology behaviour to reduce the possibility of wear rate and reduce the friction of objects, respectively. Thus, in this study, the fundamental of micro bearing concept was imparted as the Ultra High Molecular Weight Polyethylene (UHMWPE), which is a low density filler, will float onto the surface of the composite system to become a solid lubricant upon curing. UHMWPE filler, which is commonly known for its dominant properties of high tendency to resist wear and has low coefficient of friction were fabricated alongside epoxy resin in the composite system to achieve the desired strength and durability to perform over time. However, there are limitations of UHMWPE during processing upon the dispersion of the fillers with the matrix particles due to epoxy resin that has relatively high in viscosity. Therefore, acetone has been selected as a diluent with ratio of 1:1/4, 1:1/3, 1:1/2, 1:1 to dilute the high viscosity epoxy resin. The surface profile measurement were examined using Alicona Infinite Focus and Polarised Optical Microscope. Based on the results observed, EpUPE3 (epoxy and UHMWPE with acetone ratio of 1:1/2) showed better surface distribution and morphology with relatively low value of surface roughness (Ra) which is 1.41 μm and low pseuodocolour value of surface height which is around 6.76-6.77 cm compared to other formulation ratio. In near future, these surface topography and morphological analysis are important to relate with tribological, physical and mechanical properties of the micro bearing layers for bearing applications, specifically.
  10. Wan Nurhayati Wan Hanafi, Nur Syahirah Abu Bakar
    Scientific Research Journal, 2019;16(2):17-26.
    MyJurnal
    Polymerase Chain Reaction (PCR) analysis in the study of oil palm DNA generally carried out by using DNA template obtained from grinding of leaf samples in liquid nitrogen followed by hexadecyltrimethylammonium bromide (CTAB) protocol. The present study explores the FTA card as a method to retrieve PCR-amplifiable oil palm DNA. Oil palm leaves were cut and crushed before deposited onto the FTA card. An attempt was made by amplifying the EgSHP gene using a punch of FTA card as a DNA template. The successful outcome of PCR was measured by the presence of PCR amplicons on 1% agarose gel electrophoresis, indicate the genotype of oil palm fruit form. This present study demonstrates that the FTA card provides a versatile alternative to the study of oil palm genetics.
  11. Shamsatun Nahar Ahmad, Nor’Aini Aris, Azlina Jumadi
    Scientific Research Journal, 2019;16(2):1-16.
    MyJurnal
    Concepts from algebraic geometry such as cones and fans are related to toric varieties and can be applied to determine the convex polytopes and homogeneous coordinate rings of multivariate polynomial systems. The homogeneous coordinates of a system in its projective vector space can be associated with the entries of the resultant matrix of the system under consideration. This paper presents some conditions for the homogeneous coordinates of a certain system of bivariate polynomials through the construction and implementation of the Sylvester-Bèzout hybrid resultant matrix formulation. This basis of the implementation of the Bèzout block applies a combinatorial approach on a set of linear inequalities, named 5-rule. The inequalities involved the set of exponent vectors of the monomials of the system and the entries of the matrix are determined from the coefficients of facets variable known as brackets. The approach can determine the homogeneous coordinates of the given system and the entries of the Bèzout block. Conditions for determining the homogeneous coordinates are also given and proven.
  12. Nur Liyana Aifa Mahammad Asri, Ainil Idzaty Mohamed Anwar, Nur Atiqah Najib, Gisip, Judith
    Scientific Research Journal, 2019;16(1):61-77.
    MyJurnal
    Composite panels were manufactured from kenaf particles and treated with two different alkali treatments using 2% NaOH and 2% KOH with resin contents of 8% and 10% of phenol formaldehyde (PF) at medium density of 650kg/m3. The objectives of this study were to determine the mechanical properties in terms of its modulus of rupture (MOR), modulus of elasticity (MOE) and internal bond (IB), and physical properties namely thickness swelling (TS) and water absorption (WA) of treated kenaf board. The mechanical and physical tests were performed according to the Malaysian Standard (MS1787:2004). The minimum requirements value for MOE, MOR and IB were 2000 MPa, 14 MPa and 0.45 MPa respectively for furniture grade particleboards for use in humid conditions (PF2). According to Malaysian specifications for physical properties, the maximum requirement for thickness swell is 15%. Results indicated that both treated boards with NaOH and KOH showed an increase in strength properties compared to untreated particleboard. Particleboard treated with KOH exhibited the highest MOR and MOE values, while board with NaOH treatment gave the highest IB value. The boards with treated particles gave better performance in terms of physical properties. There were no significant differences in mechanical properties (MOR, MOE and IB) and physical properties for the different alkali treatment. The values of bending strength and IB strength increased with an increase in resin content, while TS and WA increased with a decrease in resin content. In conclusion, NaOH and KOH treated kenaf particles improved board performance and could be considered as an alternative material for particleboard production.
  13. Nurul Azwa Mohd Azizi, Nurul Yani Rahim, Raoov, Muggundha, Saliza Asman
    Scientific Research Journal, 2019;16(1):41-59.
    MyJurnal
    The cloud point extraction (CPE) method was developed to determine the zinc prior to Ultraviolet-visible (UV-Vis) spectrophotometry detection. Triton X-100was applied as extractant based on the complexation reaction of Zn(II) ions with ethylenediaminetetraacetic acid (EDTA). Under optimal conditions, the CPE was used to determine the concentration of zinc in canned food samples. The amounts of zinc found in the food samples were in the range of 0.005-0.007 mg/L with RSD of < 8 %. This confirmed that the proposed CPE method is suitable for the determination of zinc in food samples, indicating the concentration of zinc was within the permissible limit.
  14. M. Sulaiman M. Johari, Norizan Ahmat, Aisyah S. Kamarozaman, M. Hamizan M. Isa
    Scientific Research Journal, 2019;16(1):29-40.
    MyJurnal
    The genus Macaranga comes from the family of Euphorbiaceae and it is the only genus in the subtribe Macaranginae that have a large genus with 300 species of which 27 species were found in Peninsular Malaysia. This plant grows as shrubs or trees that can grow up to 15 m tall and known for their mutual associations with ants. Fresh or dried leaves of some Macaranga species were used by traditional healers to treat swellings, cuts, sores, boils and bruises. The isolation of chemical constituent from this genus has been shown to produce numerous results of phenolic compounds, such as flavonoids and stilbenoids. In this paper, we report the isolation of a prenylated flavonol, glyasperin A (1), together with a simple flavone apigenin (2) from the methanolic extract of the leaves of Macaranga gigantea. The structure of both compounds has been elucidated based on its spectroscopic data, including mass spectroscopy (MS), infrared (IR), ultraviolet-visible (UV-Vis), 1D and 2D nuclear magnetic resonance (NMR) spectra and comparison with the previous literature.
  15. Mohd Faiz Hassan, Chan, Kok Sheng
    Scientific Research Journal, 2019;16(1):13-27.
    MyJurnal
    The manganese oxides composite was synthesised via a simple molten salts method using a purchased MnCl2•2H2O as structuring agent. The structural details of the sample are important to be studied and previously rarely reported. It was characterised using X-ray diffraction (XRD) and data analyses including fitting process were done by Search-Match and Origin software. The Vesta software was used to draw a diagram crystal structure of the as-prepared composite. The experimental results indicated that the composite has two phases, Mn3O4 and MnO2, in value of approximately 74.22% and 25.78%, separately. The composite was highly crystalline with 74.69% compared to 25.31% for amorphous. The crystallite size of the Mn3O4 and MnO2 were calculated in an average of 22 and 24 nm, respectively. However, the lattice strain of the as-prepared composite was obtained between 7.1 x 10-3 and 7.4 x 10-3. The lattice parameters for the Mn3O4 compound was a = 5.705 Å and c = 9.473 Å, whereas a = 9.390 Å, b = 2.477 Å, c = 4.705 Å correspond to the MnO2 compound. The obtained results concluded that the composite containing two different types of system structure (tetragonal and orthorhombic).
  16. Ibtisam Abdul Wahab, Hannis Fadzillah Mohsin
    Scientific Research Journal, 2019;16(1):1-12.
    MyJurnal
    This research is about the natural product of coriander, or the Coriandrum species. The bioactive molecules and the health effects of this plant are constantly discussed. This paper is parallel with those publications, investigating the composition of the herb. The results of current study indicates that the Coriandrum seed is a source of fatty acids. From the publications, petroselinic acid or (6Z)-octadecenoic acid, is the major fatty acid in the coriander extracts. Previous articles reported on coriander’s chemical constituents, biological activities of the coriander’s seeds and leaves, as well as the coriander products in the retail. Indeed, coriander has a lot of uses either as a traditional medicines or spices. Different forms of coriander samples were subjected to a simple maceration by using chloroform. From the chromatographic purification of the seed crude extract, its component could include anisaldehyde, as one of the aromatic aldehyde. It is concluded that the lipid-containing fraction of the coriander seed could also consist of dipetroselin, a diglyceride.
  17. Johnson BT, Riemen JAJM
    Forensic Sci Res, 2019;4(4):293-302.
    PMID: 32002488 DOI: 10.1080/20961790.2018.1521327
    Identification of victims following a mass fatality is conducted by collecting and analysing a series of scientific identifiers and contextual information of each decedent. Recently, there has been a paradigm shift demanding that this complex identification process be accelerated to meet the needs of the surviving families, politicians and even the media. Postmortem fingerprint identification is a fast and efficient means of victim identification, and through the use of new advances in technology, the digital capture of fingerprints in a disaster victim identification (DVI) setting will play a strong role. This paper provides an overview of current technology and explains how this technology can adapt to current DVI procedures. The Malaysian Airlines Flight 17 (MH17) incident is a recent example of a DVI event that utilized new digital fingerprint capture technology and further demonstrates why such technology is warranted in future mass fatality operations.
  18. Mohd Hatta NNKN, Nurumal MS, Isa MLM, Daud A, Ibrahim M, Sharifudin MA, et al.
    Cent Asian J Glob Health, 2019;8(1):348.
    PMID: 32002313 DOI: 10.5195/cajgh.2019.348
    Introduction: Public awareness of osteoporosis is low among women in the developing countries. Health education was shown to be effective in improving knowledge and awareness on maintaining bone health. This study aims to identify the level of knowledge and attitudes among post-menopausal women in Malaysia on achieving bone health throughout the menopausal transition period.

    Methods: A total of 116 post-menopausal female patients of orthopedic menopause clinic were recruited using a purposive sampling approach. Data on osteoporosis awareness and knowledge were collected using validated structured questionnaires Osteoporosis Prevention and Awareness Tool and Osteoporosis Attitude Knowledge Test. The chi-square test was used to determine the association between post-menopausal women's socio-demographic characteristics and their knowledge and attitude towards maintaining bone health.

    Results: Participants' age ranged between 49 and 82 years (61.84, SD=7.87). The knowledge of osteoporosis varied significantly by age (p=0.014) and education (p=0.001) among the studied population. No significant diffrences were found for participants' attitude towards bone health.

    Conclusion: This study showed that the age and education levels have significantly different knowledge of bone health.

  19. Marzo RR, Bhattacharya S, Ravichandran S, Lakshmanan P, Jeffery VR, Moralitheran P, et al.
    PMID: 32002422 DOI: 10.4103/jehp.jehp_192_19
    INTRODUCTION: Smoking is one of the leading factors of mortality in Malaysia. Most youngsters start at adolescence, fascinated by the concept of smoking. Interventions that harness the broad availability of mobile phones, as well as adolescents' interest in their appearance, may be an innovative way to advance school-based prevention. This study aims to determine the perceptions of facial-aging apps among secondary school students.

    METHODOLOGY: For this research, descriptive cross-sectional study using simple random sampling method was used. Population sampling was targeted toward three government schools. The total number of respondents is 383, with all of them aged between 13 and- 16 years of age. Legal considerations were taken to maintain the confidentiality of respondents. The specific objectives are: 1. To determine the level of change of intention on smoking, 2. To know the perceived reactions of the peer groups on the appearances of students as nonsmokers, 3. To determine whether the students learned new benefits of nonsmokingand, 4. To measure the impact of a facial-aging app among students.

    RESULTS: The number of respondents who smoke was 40 (10.4%), while the number of respondents who do not smoke was 343 (89.6%). About 89% of the respondents agree that their three-dimensional selfie image motivates them not to smoke. In addition, 87.8% of respondents admit that the perceived reactions of their classmates make them think that they look better as nonsmokers. After learning the effects of smoking, about 86.4% of the respondents acknowledged that they would educate their peer groups. Furthermore, 85.9% of the respondents found this "Smokerface" app enjoyable.

    CONCLUSION: The facial-aging intervention was effective in motivating Malaysian pupils to stay away from tobacco use. Thus, the analysis on the study of facial app usage in smoking prevention among youngsters concludes that most of the adolescents concur that the "Smokerface" app helps in the prevention of smoking among youths.

    MeSH terms: Adolescent; Aging; Confidentiality; Cross-Sectional Studies; Government; Humans; Malaysia; Motivation; Peer Group; Smoke; Smoking; Students; Intention; Tobacco Use
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