Browse publications by year: 2019

  1. Muthu J, Muthanandam S, Sethuraman KR, Narayan KA, Ananthakrishnan N, Adkoli BV
    PMID: 32002427 DOI: 10.4103/jehp.jehp_53_19
    CONTEXT: The general dentist must not only have a broad biomedical and clinical education but also be able to demonstrate professional and ethical behavior as well as effective communication and interpersonal skills. In addition he or she must have the ability to evaluate and utilize emerging technologies, continuing professional development opportunities, and problem-solving and critical thinking skills to effectively address current and future issues in health care. But the extent to which the core competencies are taught and the students' level of proficiency in these competencies in Indian scenario is to be explored at large.

    AIMS: The present study aims at assessing the self-perceived level of competencies and their importance for future practice and the extent to which the competencies are taught in the curriculum among interns of dental college in Pondicherry, India.

    SETTINGS AND DESIGN: The cross-sectional observational study was conducted among dental interns of Indira Gandhi Institute of Dental Sciences, Pondicherry.

    SUBJECTS AND METHODS: A total of 72 interns participated in the study. A short version of the Freiburg Questionnaire to Assess Competencies in Medicine" was used in this study. Questionnaire has three sections with same set of questions, which the student had to rate: (i) To what extent do you have the following competencies at your disposal? (ii) To what extent will your future job require the following competencies? and (iii) To what extent is competencies taught to you? After the results were obtained a focused group discussion with the responders was done. Focused group discussion consisted of open questions to the groups in all the four domains and the responses of the students were scribed.

    RESULTS: In all four domains, self-perceived level of competency was not satisfactory. However, all students stated that the competencies were highly relevant for their future practice. Despite this, most of the competencies are not taught to necessary extent in the curriculum. The results of the present survey revealed that the participating students perceived deficiencies in all domains of competencies.

    CONCLUSIONS: These results indicate that the core competencies are still barely integrated into dental curricula and that further research in this field is needed.

  2. Kochuieva M, Psarova V, Ruban L, Kyrychenko N, Alypova O, Matlai O, et al.
    Wiad Lek, 2019 Aug 31;72(8):1484-1498.
    PMID: 32003208
    Introduction: The metabolic syndrome is one of the most discussed cross-disciplinary problems of modern medicine. Now there are various definitions and criteria of diagnostics of metabolic syndrome. The abdominal obesity is considered the main component of the metabolic syndrome, as a reflection of visceral obesity which degree is offered to be estimated on an indirect indicator – a waist circumference. Alongside with abdominal obesity, a number of classifications distinguish insulin resistance (IR) as a diagnostic criterion of metabolic syndrome. It is proved that IR is one of the pathophysiological mechanisms influencing the development and the course of arterial hypertension (AH), type 2 DM and obesity. There are two components in the development of IR: genetic (hereditary) and acquired. In spite of the fact that IR has the accurate genetic predisposition, exact genetic disorders of its appearance have not been identified yet, thus demonstrating its polygenic nature.

    The aim: To establish possible associations of the insulin receptor substrate-1 (IRS-1) gene polymorphism with the severity of the metabolic syndrome components in patients with arterial hypertension (AH).

    Material and methods: 187 patients with AH aged 45-55 years and 30 healthy individuals. Methods: anthropometry, reactive hyperemia, color Doppler mapping, biochemical blood analysis, HOMA-insulin resistance (IR), glucose tolerance test, enzyme immunoassay, molecular genetic method.

    Results: Among hypertensive patients, 103 had abdominal obesity, 43 - type 2 diabetes, 131 - increased blood triglycerides, 19 - decreased high density lipoproteins, 59 - prediabetes (33 - fasting hyperglycemia and 26 - impaired glucose tolerance), 126 had IR. At the same time, hypertensive patients had the following distribution of IRS-1 genotypes: Gly/Gly - 47.9%, Gly/Arg - 42.2% and Arg/Arg - 10.7%, whereas in healthy individuals the distribution of genotypes was significantly different: Gly/Gly - 86.8% (p <0.01), Gly/ Arg - 9.9% (p <0.01) and Arg/Arg - 3.3% (p <0.05). Hypertensive patients with Arg/Arg and Gly/Arg genotypes had significantly higher HOMA-IR (p <0.01), glucose, insulin and triglycerides levels (p <0.05), than in Gly/Gly genotype. At the same time, body mass index, waist circumference, blood pressure, adiponectin, HDL, interleukin-6, C-reactive protein, degree of endothelium-dependent vasodilation, as well as the frequency of occurrence of impaired glucose tolerance did not significantly differ in IRS-1 genotypes.

    Conclusions: In hypertensive patients, the genetic polymorphism of IRS-1 gene is associated with such components of the metabolic syndrome as hypertriglyceridemia and fasting hyperglycemia; it is not associated with proinflammatory state, endothelial dysfunction, dysglycemia, an increase in waist circumference and decrease in HDL.

    MeSH terms: Blood Glucose; Diabetes Mellitus, Type 2*; Humans; Insulin Resistance*; Middle Aged; Polymorphism, Genetic; Body Mass Index; Insulin Receptor Substrate Proteins/genetics*
  3. Vairavan R, Abdullah O, Retnasamy PB, Sauli Z, Shahimin MM, Retnasamy V
    Curr Med Imaging Rev, 2019;15(2):85-121.
    PMID: 31975658 DOI: 10.2174/1573405613666170912115617
    BACKGROUND: Breast carcinoma is a life threatening disease that accounts for 25.1% of all carcinoma among women worldwide. Early detection of the disease enhances the chance for survival.

    DISCUSSION: This paper presents comprehensive report on breast carcinoma disease and its modalities available for detection and diagnosis, as it delves into the screening and detection modalities with special focus placed on the non-invasive techniques and its recent advancement work done, as well as a proposal on a novel method for the application of early breast carcinoma detection.

    CONCLUSION: This paper aims to serve as a foundation guidance for the reader to attain bird's eye understanding on breast carcinoma disease and its current non-invasive modalities.

    MeSH terms: Algorithms; Breast/anatomy & histology; Breast/pathology; Breast Neoplasms/pathology; Carcinoma/pathology; Contrast Media; Diagnostic Imaging/methods; Diagnostic Imaging/trends; Female; Humans; Magnetic Resonance Imaging/methods; Mammography/methods; Mammography/trends; Medical Illustration; Neoplasm Staging/methods; Thermography/methods; Tomography, X-Ray Computed/methods; Transillumination/methods; Ultrasonography, Mammary/methods; Electric Impedance; Diffusion Magnetic Resonance Imaging/methods; Positron-Emission Tomography/methods; Elasticity Imaging Techniques/methods; Early Detection of Cancer/methods*
  4. Jatoi MA, Kamel N, Musavi SHA, López JD
    Curr Med Imaging Rev, 2019;15(2):184-193.
    PMID: 31975664 DOI: 10.2174/1573405613666170629112918
    BACKGROUND: Electrical signals are generated inside human brain due to any mental or physical task. This causes activation of several sources inside brain which are localized using various optimization algorithms.

    METHODS: Such activity is recorded through various neuroimaging techniques like fMRI, EEG, MEG etc. EEG signals based localization is termed as EEG source localization. The source localization problem is defined by two complementary problems; the forward problem and the inverse problem. The forward problem involves the modeling how the electromagnetic sources cause measurement in sensor space, while the inverse problem refers to the estimation of the sources (causes) from observed data (consequences). Usually, this inverse problem is ill-posed. In other words, there are many solutions to the inverse problem that explains the same data. This ill-posed problem can be finessed by using prior information within a Bayesian framework. This research work discusses source reconstruction for EEG data using a Bayesian framework. In particular, MSP, LORETA and MNE are compared.

    RESULTS: The results are compared in terms of variational free energy approximation to model evidence and in terms of variance accounted for in the sensor space. The results are taken for real time EEG data and synthetically generated EEG data at an SNR level of 10dB.

    CONCLUSION: In brief, it was seen that MSP has the highest evidence and lowest localization error when compared to classical models. Furthermore, the plausibility and consistency of the source reconstruction speaks to the ability of MSP technique to localize active brain sources.

    MeSH terms: Algorithms*; Bayes Theorem; Brain/physiology*; Brain Mapping/methods; Electroencephalography/methods*; Humans; Magnetic Resonance Imaging/methods*; Models, Neurological; Reproducibility of Results; Magnetoencephalography/methods*; Event-Related Potentials, P300
  5. Ismail NE, Jimam NS, Dapar MLP, Ahmad S
    Front Pharmacol, 2019;10:1521.
    PMID: 31998125 DOI: 10.3389/fphar.2019.01521
    Background: This study assessed the validity and reliability of healthcare workers' knowledge, attitudes, and practices instrument for uncomplicated malaria (HKAPIUM) for evaluation of healthcare workers' knowledge, attitudes, and practices (KAP) on uncomplicated malaria management in primary healthcare (PHC) facilities in Plateau state, Nigeria. Methods: Relevant variables from literature, malaria treatment guidelines for Nigeria, and World Health Organization (WHO) were used to generate and present the items for the draft HKAPIUM scale, which was first screened by six experts before administered to 121 respondents who filled and returned immediately. The data were sorted and analyzed using Rasch measurement model (Bond & Fox software®). Results: The outcome of the initial screening showed high items content validity indices (I-CVI) (0.83-1.00) and high scale-CVI (S-CVI) {universal agreement (UA) within the experts (S-CVI/UA) (0.67-0.89) and the average CVI [S-CVI/Ave (0.94-0.98)]} for relevance, clarity, simplicity, and comprehensiveness. The Rasch analysis outputs showed good items' reliability for the three factors (KAP) > 0.9 with high separation index values of > 2.0; however person reliability were poor (< 0.6) which were confirmed by their low separation values. Goodness of fit statistics indicated nine items not fitting the model based on the suggested fit index values of 0.6 to 1.5, and ± 2 for mean square (MNSQ) and standardized Z-score (Zstds) respectively, and 0.3 to 0.7 for "point-measure correlation coefficients" (PTMEA Corr). Deletion of misfit items resulted in the items and persons' reliabilities falling above the minimum accepted limit of 0.6, with their separation values were all in the range of 1 and 2 which were acceptable. Similarly, fit index values for MNSQ infit and outfit, and Zstd parameters items in the new scale were all within the acceptable range of 0.6 to 1.5, and ±2 respectively, in addition to the positive PTMEA Corr as further confirmation of the items' fitness to the model. Conclusion: The reduction of 27-items draft HKAPIUM scale to 18 items was successful with good reliability and fitness to the model.
    MeSH terms: Accidental Falls; Animals; Attitude; Foxes; Health Personnel; Humans; Malaria; Nigeria; Primary Health Care; Publications; Surveys and Questionnaires; Software; World Health Organization; Reproducibility of Results
  6. Chen IC, Teng G, Chen CJ, Lan TH, Liu HJ
    Front Psychiatry, 2019;10:944.
    PMID: 31998160 DOI: 10.3389/fpsyt.2019.00944
    Background: The intrinsic motivation behind the "need to complete" is more influential than external incentives. We introduced a novel progress-bar tool to motivate the completion of programs designed to treat stimulant and cannabis use disorders. We further examined the effectiveness of the progress bar's scoring approach in forecasting consistently negative urine tests. Methods: This study's participants included 568 patients with stimulant, amphetamine-type, and cannabis use disorders who were undergoing 12-month mandatory treatment programs at Taichung Veterans General Hospital in Taiwan. Patients were given scores of 1, -1, or 0 depending on whether they received negative, positive, or missing urinalysis reports, respectively. The autonomic progress bar generated weekly score totals. At the group level, scorei donated scores from all patients for a given week (i denoted the week). Scorei was standardized to adjusted scorei. We then conducted Autoregressive Integrated Moving Average (ARIMA) Model of time-series analyses for the adjusted scorei. Results: A total of 312 patients maintained treatment progress over the 12-month program. The autonomic score calculator totaled the shared achievements of these patients. The coefficients of the lag variables for mean (p), lag variables for residual error term (q), and number of orders for ensuring stationary (d) were estimated at p = 3, d = 4, and q = 7 for the first half of the treatment program, and were estimated at p = 2, d = 2, and q = 3 for the second half. Both models were stationary and tested as fit for prediction (p < 0.05). Sharply raised adjusted scores were predicted during the high-demand treatment phase. Discussion: This study's novel progress-bar tool effectively motivated treatment completion. It was also effective in forecasting continually negative urine tests. The tool's free open-source code makes it easy to implement among many substance-treatment services.
    MeSH terms: Amphetamine; Central Nervous System Stimulants; Cannabis; Hospitals, General; Hospitals, Veterans; Humans; Longitudinal Studies; Motivation; Taiwan; United States; Veterans; Urinalysis; Treatment Outcome; Moclobemide
  7. Saleem Mustafa, Abentin Estim, Syafiqah Saufi
    MyJurnal
    Biodynamics of water quality and related issues in integrated aquatic farming systems, especially the Integrated Multi-Trophic Aquaculture (IMTA), are reviewed in this paper. Combining several species in one system in addition to the microbiological organisms that become part of a production unit achieve biodynamics that is truly remarkable and mimics the processes that nature utilizes through biodiversity and interlinkages. Nutrient cascading is the most visible process in such a system. Some of the features that characterize IMTA include: harmonious functioning of multiple species, self-manuring, in tune with nature, wellbeing of captive stocks and low-carbon processes. Basically, IMTA has three loops: fed species and biofiltration, and the water quality impacted by processes in the first two loops. Maintaining homoeostasis in the system can be challenging for a number of reasons, including species-specific water quality requirements, turnover of dissolved gases (mainly oxygen and nitrogen) and particulate matter. Ammonia fluctuates with pH and temperature. Dissolved oxygen is influenced by temperature. While at neutral pH (7.0), more than 95% of ammonia is in ionized, non-toxic form (NH4+), the percentage of toxic un-ionized ammonia (NH3) increases with pH at a given temperature. NH3 is highly toxic. It produces stress at 0.1 mg/L by damaging the gills and disrupting metabolism, and death at higher concentrations. Nitrite is toxic when its concentration exceeds 0.4 mg/L. Concentration lower than this value can be fatal for more sensitive species. Process of nitrification that converts ammonia to nitrite and nitrite to nitrate requires at least 6 mg/L of dissolved oxygen. The culture system should remain well aerated, at slightly alkaline pH and moderately warm temperature, and must have substrate for nitrifying bacteria. Roles of the various types of filtering devices for organic and inorganic wastes are discussed in this paper.
  8. Muhammad Azeem Yaaqoba, Suhaily Mohd Hairon, Thirumulu Ponnuraj Kannana,, Nurhayu Ab Rahmand
    MyJurnal
    This study was to determine the sociodemographic and clinicopathological factors that were associated with mortality of OSCC patients managed at Hospital USM. The prevalence of high-risk human papillomavirus (HPV) in these patients and its association with epithelial proliferation rate were also determined. A retrospective study was conducted whereby medical records of patients diagnosed with OSCC and tissue specimens from 2005 to 2015 were studied. Formalin-fixed paraffin-embedded (FFPE) tissue specimens were evaluated for histological grading of OSCC, p16 overexpression and Ki-67 immunostaining. Descriptive statistics, simple and multiple logistics regressions were used for data analysis. Prognostic factors for mortality includes male gender (AOR=10.89; 95% CI: 1.99, 59.65; p = 0.006), alcohol consumption (AOR = 16.45; 95% CI: 1.36, 59.65; p = 0.028), not receiving treatment (AOR = 5.88; 95% CI: 1.03, 33.61; p = 0.046) and late stage (T3, T4) at presentation (AOR = 4.85; 95% CI: 1.12, 21.02; p = 0.035). Significant association was found between high-risk HPV positivity and higher epithelial proliferation rate expression (p < 0.003) in the OSCC tissue specimens.
  9. Averistusa, Gretha, Noor Hayati Abdul Razaka, Mohammad Khursheed Alam
    MyJurnal
    The present study aims to investigate and compare the hard and soft tissue cephalometric measurement among Malaysian Malay and Chinese subjects. This study is a cross-sectional study of secondary data. Data consists of 470 standardized lateral cephalometric radiographs of Malaysian Malay and Chinese adults which were picked randomly among orthodontic patients of Hospital USM. The criteria of selection were pre-treatment lateral cephalometric film of Malay and Chinese orthodontic patients aged 18-25 years which has good quality with visible landmarks. All cephalometric landmarks were located and determined and subsequently all measurements were done per COGS analysis using CASSOS software. Independent t-tests were performed for statistical comparison. Out of the 38 measurements, 4 were found significantly different between the sexes for Malaysian Chinese and 18 were found significantly different between the sexes for Malaysian Malay. Statistically significant disparities were also found between Malaysian Malay and Chinese as 16 measurements has p-value of < 0.05. Disparities were observed in COGS values between the two races and each sex group.
  10. Ying, Loo Ying, Lin, Low Mei, Lynn, Mok Hsiao, Norliwati Ibrahim, Asma Alhusna Abang Abdullah
    MyJurnal
    Oral candidiasis is an opportunistic infection of the oral cavity. This study aimed to determine prevalence of oral candidiasis in patients with palatal coverage orthodontic appliances and prostheses and its risk factors. Three groups of patients were recruited after an informed consent. The two tested groups were the denture and the upper removable orthodontic appliance (URA) groups while patients with no prosthesis/appliance acted as control. The assessments included demographic profile, denture/URA age, night-time wearing and duration of wearing. Signs and symptoms of candidiasis were examined intra-orally. Unstimulated salivary flow rate test was also performed. Smears samples were taken from palatal mucosa and impression surfaces of denture/URA and stained with Periodic Acid Schiff (PAS). The data were analysed using descriptive and chi-square tests. A total of 86 patients were recruited; denture (n=30), URA (n=22), control (n=34). Denture and URA groups had significantly more positive cases of candidal infection (56.7 and 72.7% respectively) as compared to control group (20.6%). Among the subjects with oral candidiasis, denture patients were detected to have more severe amount of candidal hyphae (20%) than URA (13.6%) group. The relationships between oral candidiasis and these risk factors i.e. age, night-time wearing, daily total hours of wearing and denture/URA hygiene were statistically significant (p
  11. Fatin Hazwani Fauzia, Nurul Izzati Hamzana, Nurhayu Ab. Rahmana, Irfan Mohamadb, Siti Suraiya, Thomas George Kallarakkald, et al.
    MyJurnal
    Infection of the oral cavity with high-risk human papillomavirus (HPV) has been implicated as one of the risk factors for the development of oral squamous cell carcinoma (OSCC). Among the high-risk HPV types, HPV 16 and 18 are the most common infective agents in oral cancers. This study aimed to compare the presence of high-risk HPV in genetic materials obtained from saliva, blood and tissues of OSCC patients in Malaysia. The genomic DNA was extracted from saliva (n=13), blood (n=59) and tissue (n=63) and subjected to polymerase chain reaction (PCR) amplification of human beta globin gene to confirm the presence and integrity of DNA. Positive amplification was then screened for high-risk HPV by nested PCR using MY11/09 and GP5+/6+ consensus primers, followed by a further confirmation by DNA sequencing of the positive samples. As a result, two saliva samples (2/13; 15.4%) were found to harbour HPV 16 and one tissue sample (1/63; 1.6%) was shown to be positive for HPV 18. However, none of the blood samples were positive for high-risk HPV. Thus, HPV is more likely to be found in the saliva of OSCC patients as compared to blood and tissue samples. The detection of high-risk HPV in OSCC patients is useful in deciding how to manage the patient as HPV-associated OSCC has better prognosis.
  12. Tuan Siti Mastazliha Long Tuan Kechik, Zurairah Berahim, Wan Nazatul Shima Shahidan
    MyJurnal
    Human salivary exosomes have been identified as a highly informative nanovesicle with clinical-relevant information for variation of diagnostic purposes. As a continued effort from previous studies on human salivary exosomes effect at gene expression level, this study is carried out to observe the morphology of human periodontal fibroblast (HPdLF) treated with exosomes cells under the same period of changes in genotypic level occurred. In vitro, HPdLF cells were cultured for 24 hours with 10 μg/ml of human salivary exosomes. The morphology of HPdLF cells was examined under inverted light microscopy and scanning electron microscopy (SEM) for both control samples and samples treated with human salivary exosomes, while the cell count was performed via trypan blue staining. There was no significant difference in the morphology under the inverted light microscopy and the cell number of HPdLF cells for both treated and untreated cells with exosomes. However, for SEM, the treated HPdLF with salivary exosomes showed slight observable changes on the filopodia, lamellipodia, cytoplasmic vesicles and the cytoskeleton of the cells. Even within a short period (24 hours) of culturing time for cells with human salivary exosomes, the samples showed minimal changes which positively suggested a simultaneous event of exchanging materials from human salivary exosomes to cells had occurred, hence, potentially proving that human salivary exosomes can enhance cell proliferation.
  13. Wong, Lishena,, Liew, Amy Kia Cheen, Dalia Abdullah, Chong, Bun San
    MyJurnal
    The study aimed to compare the scope and pattern of practice between general dental practitioners (GDPs), restorative dentistry specialists and endodontists. Self-administered postal questionnaires were distributed to 22 restorative dentistry specialists, 16 endodontists and a random sample of 566 GDPs. The pre-tested questionnaire inquired about demographic data, endodontic practices and referrals. Fisher’s exact test with Bonferroni adjustment was performed for pairwise comparisons. The overall response rate was 73.8%. The restorative dentistry specialists and the endodontists performed a wider array of endodontic procedures than the GDPs and were more consistent in the use of specific armamentarium (p
  14. Murugesu S, Khatib A, Ahmed QU, Ibrahim Z, Uzir BF, Benchoula K, et al.
    Toxicol Rep, 2019;6:1148-1154.
    PMID: 31993329 DOI: 10.1016/j.toxrep.2019.10.020
    Clinacanthus nutans, an herbal shrub belonging to the Acanthaceae family, is traditionally used as a functional food to treat various ailments in Malaysia and Indonesia. Although the polar fraction of this plant shows non-toxic effect, the toxicity of the non-polar extract is not reported so far. The present study aimed to assess the toxic effect and determine the lethal concentration of this non-polar fraction using zebrafish embryos. The n-hexane fraction was partitioned from the crude extract of C. nutans obtained using 80% methanolic solution. After spawning of the adult male and female zebrafish, the eggs were collected, transferred into a 96-well plate and incubated with the n-hexane fraction at concentrations of 15.63 μg/ml, 31.25 μg/ml, 62.5 μg/ml, 125 μg/ml, 250 μg/ml and 500 μg/ml in 2% DMSO. The survival and sublethal endpoint were assessed, the mortality and hatchability rates were calculated based on microscopic observation, while the heartbeat rate was measured using DanioScope software. The median lethal concentration (LC50) of the C. nutans n-hexane fraction, which was determined using probit analysis, was calculated to be 75.49 μg/mL, which is harmful. Moreover, gas chromatography-mass spectrometry (GC-MS) analysis revealed the presence of palmitic acid, phytol, hexadecanoic acid, 1-monopalmitin, stigmast-5-ene, pentadecanoic acid, heptadecanoic acid, 1-linolenoylglycerol and stigmasterol in the n-hexane fraction.
    MeSH terms: Animals; Dimethyl Sulfoxide; Fatty Acids; Female; Indonesia; Malaysia; Male; Gas Chromatography-Mass Spectrometry; Phytol; Stigmasterol; Zebrafish; Palmitic Acid; Acanthaceae; Functional Food
  15. Tagami T, Tanaka H, Shin SD, Ma MH, Ko PC, Karim S, et al.
    Acute medicine & surgery, 2019 05 22;7(1):e430.
    PMID: 31988755 DOI: 10.1002/ams2.430
    Aim: As a population ages, it can impact on the characteristics and outcomes of cardiogenic out-of-hospital cardiac arrest (OHCA) patients. This study aimed to evaluate the relationship between the age incidence of cardiogenic OHCA and population aging.

    Methods: This was a post-hoc analysis of the Pan Asian Resuscitation Outcomes Study (PAROS) database. Data on the population old-age dependency ratio (i.e. elderly/non-elderly) were extracted from publicly accessible sources (United Nations and World Health Organization).

    Results: We analyzed 40,872 OHCA cases from seven PAROS countries over the period 2009 to 2013. We found significant correlation between the population old-age dependency ratio and elderly/non-elderly ratio in OHCA patients (r = 0.92, P = 0.003). There was a significant correlation between the population old-age dependency ratio and risk differences of 30-day survival rates for non-elderly and elderly OHCA patients (r = 0.89, P = 0.007).

    Conclusions: Our findings suggest that the proportion of elderly among OHCA patients will increase, and outcomes could increasingly differ between elderly and non-elderly as a society ages progressively. This has implications for planning and delivery of emergency services as a society ages.

    MeSH terms: Aged; Aging; Asia; Emergency Medical Services; Humans; Middle Aged; Resuscitation; World Health Organization; Incidence; Survival Rate; Out-of-Hospital Cardiac Arrest
  16. Khan SU, Ullah N, Ahmed I, Ahmad I, Mahsud MI
    Curr Med Imaging Rev, 2019;15(3):243-254.
    PMID: 31989876 DOI: 10.2174/1573405614666180726124952
    BACKGROUND: Medical imaging is to assume greater and greater significance in an efficient and precise diagnosis process.

    DISCUSSION: It is a set of various methodologies which are used to capture internal or external images of the human body and organs for clinical and diagnosis needs to examine human form for various kind of ailments. Computationally intelligent machine learning techniques and their application in medical imaging can play a significant role in expediting the diagnosis process and making it more precise.

    CONCLUSION: This review presents an up-to-date coverage about research topics which include recent literature in the areas of MRI imaging, comparison with other modalities, noise in MRI and machine learning techniques to remove the noise.

    MeSH terms: Machine Learning*; Diagnostic Imaging/methods*; Female; Humans; Magnetic Resonance Imaging/methods*; Male; Sensitivity and Specificity; Tomography, X-Ray Computed/methods; Artifacts*
  17. Ali TH, Heidelberg T, Hussen RSD, Tajuddin HA
    Curr Org Synth, 2019;16(8):1143-1148.
    PMID: 31984920 DOI: 10.2174/1570179416666191105152714
    BACKGROUND: High efficiency in terms of reaction yield and purity has led to the extensive utilization of copper-catalyzed azide-alkyne cycloaddition (CuAAC) in various fields of chemistry. Its compatibility with low molecular weight alcohols promotes the application in surfactant synthesis to tackle the miscibility constraints of the reactants.

    OBJECTIVE: For the tuning of surfactant properties, double click coupling of the antipode precursors was attempted. Failure of the CuAAC to provide the targeted product in combination with unexpected reaction outputs led to an investigation of the side reaction.

    METHODS: The CuAAC-based coupling of sugar azide with propargyl building block in the presence of copper- (I) catalyst exclusively led to the mono-coupling product in a respectable yield of almost 80%. Besides the unexpected incomplete conversion, the loss of the remaining propargyl group, as indicated by both NMR and MS. On the other hand, application of substantial amounts of CuSO4 under reducing conditions in refluxing toluene/water furnished the alkyne dimer in a moderate yield of 43%, while no change of azide compound was noticed.

    RESULTS: The Cu(I)-catalyst applied for azide-alkyne cycloadditions enables the homo-coupling of certain terminal alkynes at a higher temperature. Moreover, aromatic propargyl ethers may be cleaved to furnish the corresponding phenol. The copper-catalyzed coupling appeared highly sensitive towards the alkyne compound. Only selected derivatives of propargyl alcohol were successfully dimerized.

    CONCLUSIONS: The observed failure of the Huisgen reaction for the synthesis of sugar-based surfactants may indicate non-recognized constrains of the reaction, which could affect its wide application in bioconjugation. The temperature requirement for the alternative dimerization of terminal alkynes renders this side reaction nonrelevant for typical click couplings, while narrow substrate diversity and moderate yield limit its synthetic application.

  18. Murthy S, Hazli UHAM, Kong KW, Mai CW, Leong CO, Rahman NA, et al.
    Curr Org Synth, 2019;16(8):1166-1173.
    PMID: 31984923 DOI: 10.2174/1570179416666191003095253
    BACKGROUND: Sesamol is a widely used antioxidant for the food and pharmaceutical industries. The oxidation products of this compound may be accumulated in foods or ingested. Little is known about its effect on human health.

    OBJECTIVE: It is of great interest to identify the oxidation products of sesamol that may be beneficial to humans. This study was undertaken to identify the oxidation products of sesamol and investigate their antioxidant and cytotoxic activities.

    MATERIALS AND METHODS: Using the ferricyanide oxidation approach, four oxidation products of sesamol (2, 3, 20 & 21) have been identified. Structural elucidation of these compounds was established on the basis of their detailed NMR spectroscopic analysis, mass spectrometry and x-ray crystallography. Additionally, a formation mechanism of compound 20 was proposed based on high-resolution mass spectrometry-fragmentation method. The antioxidant activities of these compounds were determined by the DPPH, FRAP, and ABTS assays. The in vitro antiproliferative activity of these compounds was evaluated against a panel of human cancer cell lines as well as non-cancerous cells.

    RESULTS: Two oxidation products of sesamol were found to contain an unusual methylenedioxy ring-opening skeleton, as evidenced by spectroscopic and x-ray crystallographic data. Among all compounds, 20 displayed impressive antiproliferative activities against a panel of human cancer cell lines yet remained non-toxic to noncancerous cells. The antioxidant activities of compound 20 are significantly weaker than sesamol as determined by the DPPH, FRAP, and ABTS assays.

    CONCLUSION: The oxidation products of sesamol could be a valuable source of bioactive molecules. Compound 20 may be used as a potential lead molecule for cancer studies.

    MeSH terms: Antineoplastic Agents/pharmacology; Antineoplastic Agents/chemistry; Antioxidants/pharmacology; Antioxidants/chemistry*; Cell Line; Ferricyanides/chemistry; Humans; Oxidation-Reduction; Phenols/pharmacology; Phenols/chemistry*; Molecular Structure; Dimerization; Cell Proliferation/drug effects; Benzodioxoles/pharmacology; Benzodioxoles/chemistry*
  19. Nazir M, Abbasi MA, Aziz-Ur-Rehman -, Siddiqui SZ, Ali Shah SA, Shahid M, et al.
    Pak J Pharm Sci, 2019 Nov;32(6):2585-2597.
    PMID: 31969290
    In the study presented here, the nucleophilic substitution reaction of 5-[3-(1H-indol-3-yl)propyl]-1,3,4-oxadiazol-2-ylhydrosulfide was carried out with different alkyl/aralkyl halides (5a-r) to form its different S-substituted derivatives (6a-r), as depicted in scheme 1. The structural confirmation of all the synthesized compounds was done by IR, 1H-NMR, 13C-NMR and CHN analysis data. Bacterial biofilm inhibitory activity of all the synthesized compounds was carried out against Bacillus subtilis and Escherichia coli. The anticancer activity of these molecules was ascertained using anti-proliferation (SRB) assay on HCT 116 Colon Cancer Cell lines while the cytotoxicity of these molecules was profiled for their haemolytic potential. From this investigation it was rational that most of the compounds exhibited suitable antibacterial and anticancer potential along with a temperate cytotoxicity.
    MeSH terms: Anti-Bacterial Agents/adverse effects; Anti-Bacterial Agents/chemical synthesis*; Anti-Bacterial Agents/therapeutic use; Antineoplastic Agents/adverse effects; Antineoplastic Agents/chemical synthesis*; Antineoplastic Agents/therapeutic use; Bacillus subtilis/drug effects; Escherichia coli/drug effects; Humans; Indoles/adverse effects; Indoles/chemical synthesis*; Indoles/therapeutic use; Structure-Activity Relationship; Biofilms; HCT116 Cells/drug effects
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