Browse publications by year: 2020

  1. Chin JJ, Tham HW
    Front Genet, 2020;11:512582.
    PMID: 33343613 DOI: 10.3389/fgene.2020.512582
    Genetic testing aids patients in making important decisions in the prevention, treatment, or early detection of hereditary disorders. Low awareness of the importance of genetic testing contributes to the increase in the incidence of hereditary disorders. This study aims to explore the knowledge, awareness, and perception of genetic testing for hereditary disorders among local residents of the Klang Valley, Malaysia, and the potential variables that influence their understanding of genetic testing. A survey was conducted in different municipalities of the Klang Valley through self-administered questionnaire assessing the public's knowledge, awareness, and perception of genetic testing. Overall, the results revealed adequate knowledge and positive awareness of genetic testing, in which both were influenced by the respondent's educational level (P < 0.001), field of study (P < 0.001), and status of heard or unheard of genetic testing (P < 0.001). The perception of genetic testing was generally positive and influenced by the respondent's differences in age (P < 0.016), educational level (P < 0.001), field of study (P < 0.001), and status of heard or unheard of genetic testing (P < 0.001). Although positive responses were obtained, ~20.2% of the respondents had never heard of genetic testing. Of the respondents, 24.5% were unwilling to undergo genetic testing, with 25.1% believing that genetic testing tampers with nature and 18% believing that it opposes religion and their beliefs. Such attitude calls for the need to conduct programs to eliminate any misconception, as well as to educate the public to lessen any perceived misunderstanding of the concepts of genetic testing.
    MeSH terms: Cities; Decision Making; Genetic Testing; Humans; Malaysia; Surveys and Questionnaires; Incidence; Knowledge
  2. Alkadhimi A, Dawood OT, Hassali MA
    Pharm Pract (Granada), 2020 12 12;18(4):2095.
    PMID: 33343772 DOI: 10.18549/PharmPract.2020.4.2095
    Objective: This study aimed to understand the dispensing practice of antibiotics in community pharmacy in Iraq, in addition to explore the community pharmacists' perception about dispensing antibiotics without prescription.

    Methods: A qualitative design was conducted on community pharmacists in Baghdad, Iraq. Pharmacists were selected conveniently from different gender, age group, pharmacy type and years of experience. Face-to-face semi-structure interview was used with all the pharmacists to get in-depth understanding about their dispensing practice of antibiotics without prescription in community pharmacy. The data was coded and classified for thematic analysis.

    Results: This study found that dispensing of antibiotics without prescription was a common practice in community pharmacy. Pharmacists' perception towards dispensing antibiotics without prescription was associated with the medical condition, safety and efficacy of antibiotics, patients request antibiotics by name, emergency cases, regular customer, promotions from pharmaceutical companies, saving time and cost, brand medications, and poor healthcare services. In addition, there were inadequate knowledge about antibiotic resistance and lack of awareness about antibiotic stewardship leading to inappropriate dispensing practice.

    Conclusions: Community pharmacists have poor perception towards dispensing antibiotics without prescription. Educational interventions about antibiotics use focusing on community pharmacists are needed. This will help to optimize the practice of dispensing of antibiotics in the community. In addition, training programs about antibiotic resistance are important to enhance pharmacists' understanding about antibiotic stewardship.

    MeSH terms: Anti-Bacterial Agents; Drug Resistance, Microbial; Humans; Iraq; Pharmaceutical Services; Pharmacies; Pharmacists; Pharmacy; Prescriptions
  3. Kamar Affendi NH, Ahmad R, Vahidi F, Hassan MZ, Rahimi SN
    Case Rep Dent, 2020;2020:8883156.
    PMID: 33343944 DOI: 10.1155/2020/8883156
    Introduction: A diode laser is one of the universally compact accepted laser systems used fundamentally for soft tissue applications. Most diode laser devices have a single wavelength of either 810 nm for superior coagulation or 980 nm for tissue ablation. In these case series, the use of dual wavelengths (810 nm and 980 nm) in combination with super pulsing has provided a cleaner cut (no charring) with faster healing that eases the placement of the final restoration in the esthetic zone. Case Description. The present case series describe four cases in the esthetic zone that achieved hemostasis ablation without collateral damage to enhance gingival balance of definitive restoration. The gingivoplasty and gingivectomy modes are used to achieve efficient tissue ablation. Although there is no specific mode indicated in the FDA laser requirement for gingival depigmentation, the procedure could be safely performed with the dual-wavelength diode laser.

    Result: All four patients revealed a good esthetic outcome and reported no pain postoperatively. Healing was uneventful, and definitive restoration was delivered within two to four weeks postoperatively.

    Conclusion: Within the limitation of these case series, the dual-wavelength super pulsed diode laser has the capacity to deliver peak powers resulting in efficient cutting and less tissue charring and also as an alternative tool for removal of gingival pigmentation. Prospective clinical research with larger sample size is needed for conclusive results.

    MeSH terms: Esthetics; Gingiva; Gingival Diseases; Gingivectomy; Gingivoplasty; Hemostasis; Humans; Pain, Postoperative; Pigmentation; Prospective Studies; Sample Size; Lasers, Semiconductor
  4. Ramachandra SS, Gupta VV, Muttalib KA
    J Oral Biol Craniofac Res, 2020 11 10;11(1):1-4.
    PMID: 33344152 DOI: 10.1016/j.jobcr.2020.11.001
    Introduction/Problem: Clinical experience in cases of advanced complexity/rare cases is limited among undergraduate dental students. This commentary narrates a module termed "case sharing", wherein a small group of undergraduate dental students treat/assist, document and present advanced or rare cases to their entire cohort in eight weeks.

    Approach: Undergraduate students perform procedures of straightforward and moderate complexity, and later assisted the clinical specialists during procedures of advanced complexity. students document these cases with clinical photographs and case notes to make presentations that were uploaded in the faculty's online management system to be reviewed by the entire cohort. student groups presented their cases with their entire cohort. an independent assessor assessed the groups for their organization of the case, information collected on the topic, critical analysis of the case, defending the diagnosis and treatment plan, teamwork and presentation skills.

    Evaluation: Students reported improvement in the depth of knowledge on particular diseases/procedures, a better understanding of holistic management of advanced cases, improved rapport, team spirit and communication among group members. they also reported difficulties in recruiting and completing the procedures within eight weeks.

    Discussion: Apart from improving the clinical experience of undergraduate students, the module provides an opportunity for the development of teamwork, communication skills, and ethical presentations among students, which are invaluable to the faculty to meet its programme learning outcomes. case sharing provides a platform for holistic learning and serves as an alternative learning method aside from didactic lectures and routine clinical supervision.

    MeSH terms: Communication; Faculty; Humans; Learning; Specialization; Students, Dental; Cohort Studies; Narration
  5. Sun Z, He G, Huang N, Chen H, Zhang S, Zhao Z, et al.
    Front Public Health, 2020;8:609974.
    PMID: 33344408 DOI: 10.3389/fpubh.2020.609974
    Background: COVID-19 developed into a global pandemic in 2020 and poses challenges regarding the prevention and control capabilities of countries. A large number of inbound travelers from other regions could lead to a renewed outbreak of COVID-19 in the local regions. Globally, as a result of the imbalance in the control of the epidemic, all countries are facing the risk of a renewed COVID-19 outbreak brought about by travelers from epidemic areas. Therefore, studies on a proper management of the inbound travelers are urgent. Methods: We collected a total of 4,733,414 inbound travelers and 174 COVID-19 diagnosed patients in Yunnan province from 21 January 2020 to 20 February 2020. Data on place of origin, travel history, age, and gender, as well as whether they had suspected clinical manifestations for inbound travelers in Yunnan were collected. The impact of inbound travelers on the local epidemic was analyzed with a collinear statistical analysis and the effect of the control measures on the epidemic was evaluated with a sophisticated modeling approach. Results: Of the 174 COVID-19 patients, 60.9% were not from Yunnan, and 76.4% had a history of travel in Hubei. The amount of new daily cases in Yunnan was significant correlated with the number of inbound travelers from Hubei and suspected cases among them. Using Susceptible-Exposed-Infectious-Recovered (SEIR) model analysis, we found that the prevention and control measures dropped the local R0 down to 1.07 in Yunnan province. Conclusions: Our preliminary analysis showed that the proper management of inbound travelers from outbreak areas has a significantly positive effect on the prevention and control of the virus. In the process of resettlement, some effective measures taken by Yunnan province may provide an important reference for preventing the renewed COVID-19 outbreak in other regions.
    MeSH terms: China; Communicable Diseases; Disease Outbreaks; Humans; Travel; Pandemics
  6. Rufai Y, Chandren S, Basar N
    Front Chem, 2020;8:597980.
    PMID: 33344417 DOI: 10.3389/fchem.2020.597980
    Nanotechnology is one of the most interesting areas of research due to its flexibility to improve or form new products from nanoparticles (NPs), and as a fast, greener, more eco-friendly and sustainable solution to technological and environmental challenges. Among metal oxides of photocatalytic performance, the use of titania (TiO2) as photocatalyst is most popular due to its unique optical and electronic properties. Despite the wide utilization, the synthesis of TiO2 NPs bears many disadvantages: it utilizes various less environmental-friendly chemicals, high cost, requires high pressure and energy, and potentially hazardous physical and chemical methods. Hence, the development of green synthesis approach with eco-friendly natural products can be used to overcome these adverse effects. In this work, TiO2 NPs have been prepared by using Deinbollia pinnata leaves extracts, obtained by different solvents (n-hexane, ethyl acetate, and ethanol) with different polarities. The extracts acted as the reducing agent, while titanium isopropoxide as the precursor and water as the solvent. X-ray diffraction (XRD) pattern confirmed the synthesized TiO2 consist of anatase phase in high purity, with average crystallite size in the range of 19-21 nm. Characterization by using field emission scanning electron microscopy (FESEM) showed the TiO2 NPs possess a uniform semi-spherical shape in the size range of 33-48 nm. The energy dispersive X-ray (EDX) spectra of green TiO2 NPs showed two peaks for the main elements of Ti (61 Wt.%) and O (35 Wt.%). The band-gap energy of 3.2 eV was determined using UV-Vis spectroscopy. From the nitrogen sorption analysis, type V isotherm of the material was obtained, with BET surface area of 31.77 m2/g. The photocatalytic activity of synthesized TiO2 was evaluated for photodegradation of methyl orange (MO) under UV light irradiation. Based on the results, it is shown that TiO2 NPs synthesized with D. pinnata leaves extracted using ethyl acetate showed the most effective photodegradation performance, achieving 98.7% of MO conversion within 150 min. It can be concluded that the use of plant extracts in synthesis with TiO2 managed to produce highly crystalline anatase TiO2 with superior photocatalytic activity in the photodegradation of organic dye.
    MeSH terms: Animals; Ursidae; Microscopy, Electron, Scanning; Nitrogen; Photolysis; Solvents; Titanium; Ultraviolet Rays; Water; X-Ray Diffraction; X-Rays
  7. Curren E, Leaw CP, Lim PT, Leong SCY
    Front Bioeng Biotechnol, 2020;8:562760.
    PMID: 33344429 DOI: 10.3389/fbioe.2020.562760
    Microplastic pollution is a global issue that has a detrimental impact on food safety. In marine environments, microplastics are a threat to marine organisms, as they are often the same size range as prey and are mistaken as food. Consumption of microplastics has led to the damage of digestive organs and a reduction in growth and reproductive output. In this study, microplastic pollution was assessed across three commercially available shrimp species that were obtained from the supermarkets of Singapore. A total of 93 individuals were studied from the Pacific white leg shrimp, Litopenaeus vannamei, the Argentine red shrimp Pleoticus muelleri and the Indian white shrimp Fenneropenaeus indicus. Microplastic fibers, fragments, film and spheres were identified from the digestive tract of these organisms. Microplastic abundance ranged from 13.4 to 7050 items. F. indicus exhibited the highest number of microplastics. Microplastic film was the most abundant in L. vannamei individuals (93-97%) and spheres were the most abundant in P. muelleri (70%) and F. indicus (61%) individuals. This study demonstrates that microplastic contamination is evident in commonly consumed shrimp and highlights the role of shrimp in the trophic transfer and accumulation of microplastics in seafood. The consumption of microplastic-containing seafood is a route of exposure to humans and has implications on human health and food security. Capsule: Microplastics were examined in three shrimp species from the supermarkets of Singapore. Microplastics ranged from 13.4 to 7050 items of shrimp.
    MeSH terms: Food Supply; Humans; Leg; Plastics; Singapore; Seafood; Gastrointestinal Tract; Aquatic Organisms; Food Safety
  8. Naes SM, Ab-Rahim S, Mazlan M, Abdul Rahman A
    Biomed Res Int, 2020;2020:5197626.
    PMID: 33344638 DOI: 10.1155/2020/5197626
    Equilibrative nucleoside transporter 2 (ENT2) is a bidirectional transporter embedded in the biological membrane and is ubiquitously found in most tissue and cell types. ENT2 mediates the uptake of purine and pyrimidine nucleosides and nucleobase besides transporting a variety of nucleoside-derived drugs, mostly in anticancer therapy. Since high expression of ENT2 has been correlated with advanced stages of different types of cancers, consequently, this has gained significant interest in the role of ENT2 as a potential therapeutic target. Furthermore, ENT2 plays critical roles in signaling pathway and cell cycle progression. Therefore, elucidating the physiological roles of ENT2 and its properties may contribute to a better understanding of ENT2 roles beyond their transportation mechanism. This review is aimed at highlighting the main roles of ENT2 and at providing a brief update on the recent research.
    MeSH terms: Biological Transport; Cell Cycle; Neoplasms; Nucleosides; Purines; Pyrimidine Nucleosides; Signal Transduction; Equilibrative-Nucleoside Transporter 2
  9. Pramanik A, Basak AK, Littlefair G, Debnath S, Prakash C, Singh MA, et al.
    Heliyon, 2020 Dec;6(12):e05554.
    PMID: 33344787 DOI: 10.1016/j.heliyon.2020.e05554
    Titanium alloys are difficult to machine using conventional methods, therefore, nonconventional processes are often chosen in many applications. Electrical discharge machining (EDM) is one of those nonconventional processes that is used frequently for shaping titanium alloys with their respective pros and cons. However, a good understanding of this process is very difficult to achieve as research results are not properly connected and presented. Therefore, this study investigates different types of EDM processes such as, wire EDM, die-sink EDM, EDM drill and hybrid EDM used to machine titanium alloys. Machining mechanism, tool electrode, dielectric, materials removal rate (MRR), and surface integrity of all these processes are critically analysed and correlated based on the evidence accessible in literature. Machining process suffer from lower material removal rate and high tool wear while applied on titanium alloys. Formation of recast layer, heat affected zone and tool wear is common in all types of EDM processes. Additional challenge in wire EDM of titanium alloys is wire breakage under severe machining conditions. The formation of TiC and TiO2 are noticed in recast layer depending on the type of dielectrics. Removal of debris from small holes during EDM drilling is a challenge. All these restricts the applications EDMed titanium alloys in high-tech applications such as, aerospace and biomedical areas. Most of these challenges come up due to extraordinary properties such as, low thermal conductivity, high melting point and high hardness, of titanium alloys. Though hybrid EDM has been introduced and there is some work on simulation of EDM process, further developments in EDM of this alloy is required for widening the application of this methods.
    MeSH terms: Alloys; Electric Wiring; Electricity; Electrodes; Hardness; Hot Temperature; Thermal Conductivity; Titanium
  10. James C, Girard O
    PMID: 33345057 DOI: 10.3389/fspor.2020.00066
    Repeated-sprint training in hypoxia (RSH) studies conducted "in-season" are scarce. This study investigated the effect of discontinuous, running-based RSH, on repeated-sprint treadmill performance in hypoxia in a team sport cohort, prior to international competition. Over a 6-week "in-season" period, 11 elite male players (Malaysia national team) completed eight multi-set RSH sessions on a non-motorized treadmill in a normobaric hypoxic chamber (FiO2 = 13.8%). Three testing sessions (Sessions 1, 5, and 8), involved three sets of 5 × 8-s sprints, with 52-s recovery between sprints and 4-5 min between sets. Training sessions (Sessions 2, 3, 4, 6, and 7) consisted of four to five sets of 4-5 × 8-s sprints. During testing sessions, maximum sprinting speed was recorded for each sprint with values averaged for each set. For each set, a peak speed and fatigue index were calculated. Data were compared using two-way repeated measures ANOVA (sessions × sets). Average speed per set increased between testing sessions (p = 0.001,


    η


    p


    2


    = 0.49), with higher values in Session 8 (25.1 ± 0.9 km.h-1, +4 ± 3%, p = 0.005), but not Session 5 (24.8 ± 1.0 km.h-1, +3 ± 3%, p = 0.405), vs. Session 1 (24.2 ± 1.5 km.h-1). Peak sprinting speed in each set also increased across testing sessions (p = 0.008,


    η


    p


    2


    = 0.382), with Session 8 (26.5 ± 1.1 km.h-1) higher than Session 5 (25.8 ± 1.0 km.h-1, +1 ± 4%, p = 0.06) and Session 1 (25.7 ± 1.5 km.h-1, +4 ± 4%, p = 0.034). Fatigue index differed between sessions (p = 0.04,


    η


    p


    2


    = 0.331, Session 1; -6.8 ± 4.8%, Session 5; -3.8 ± 2%, Session 8; -5.3 ± 2.6%). In international field hockey players, a 6-week in-season RSH program improved average and peak, repeated treadmill sprint speeds following eight, but not five sessions.
    MeSH terms: Analysis of Variance; Exercise Test; Fatigue; Hockey; Malaysia; Male; Running; Seasons; Cohort Studies
  11. Singh VA
    J Orthop Surg (Hong Kong), 2020 12 22;28(3):2309499020972483.
    PMID: 33345709 DOI: 10.1177/2309499020972483
    MeSH terms: Hip Prosthesis*; Humans; Bone Transplantation; Arthroplasty, Replacement, Hip*; Cortical Bone
  12. Cong CW, Ling WS, Fitriana M
    J Child Adolesc Ment Health, 2020 12 22;32(2-3):131-140.
    PMID: 33345736 DOI: 10.2989/17280583.2020.1848852
    Background and aim: Adolescent suicide has become a central issue around the world, including in Malaysia, which needs attention. The current study investigated the mediating effect of coping strategy in the association between family functioning and suicidal ideation among adolescents in Kuala Lumpur, Malaysia. Method: A total of 852 school-attending adolescents aged 13-17 years were recruited by multistage cluster sampling. The relationships between all the study variables were analysed using Pearson's correlation. Moreover, the mediation model was tested using SPSS PROCESS macro, while sex differences in suicidal ideation were examined using independent samples t-test. Results: Results showed that family cohesion, family flexibility, and problem-focused coping negatively correlated with adolescents' suicidal ideation. Problem-focused coping also mediated the association between family flexibility and suicidal ideation. There was a significant difference in suicidal ideation for males and females. Conclusion: Family functioning and coping strategy are related to adolescents' suicidal ideation, while problem-focused coping plays a crucial role in the relationship between family flexibility and suicidal ideation.
    MeSH terms: Adaptation, Psychological*; Adolescent; Adolescent Behavior/psychology*; Family/psychology*; Female; Humans; Malaysia; Male; Sex Factors; Suicidal Ideation*
  13. Periasamy U, Mohd-Sidik S, Akhtari-Zavare M, Rampal L, Ismail SIF, Mahmud R
    Iran J Public Health, 2020 Oct;49(10):1902-1911.
    PMID: 33346212 DOI: 10.18502/ijph.v49i10.4693
    Background: We aimed to assess whether "Managing Patients on Chemotherapy" book is effective to improve quality of life (QOL) of cancer patient via counselling by pharmacist.

    Methods: A randomized control trial study was run among 2120 cancer patients in public hospitals in Peninsular Malaysia, from Apr 2016-Jan 2018. The treatment group received counselling regarding chemotherapy by using developed module. The data were collected at three time-points: baseline, 1st, 2nd and 3rd follow-ups after counseling by Validated Malay version of the WHOQOL-BREF of questionnaire. Data analyses were done using χ2 and two-way repeated measure ANOVA.

    Results: The treatment group improved significantly as compared to control group in physical health, psychological health, social relationship, environment and overall QOL (P<0.00).

    Conclusion: The "Managing Patients on Chemotherapy" book along with repetitive counselling by pharmacists is a useful intervention for improving QOL of cancer patients undergoing treatment.

    MeSH terms: Analysis of Variance; Books; Counseling; Hospitals, Public; Humans; Malaysia; Mental Health; Neoplasms; Pharmacists; Quality of Life; Surveys and Questionnaires; Control Groups
  14. Farizan NH, Sutan R, Mani KK
    Iran J Public Health, 2020 Oct;49(10):1921-1930.
    PMID: 33346223 DOI: 10.18502/ijph.v49i10.4695
    Background: We aimed to assess the effectiveness of the health educational booklet intervention in improving parents/guardian's knowledge on prevention of child drowning and, the perception of drowning risk and water safety practice.

    Methods: A quasi-experimental study was conducted in year 2017 in Selangor, Malaysia among 719 parents/guardians of primary school children. The parent/guardians were randomly assigned as the intervention groups and were given a health educational Be-SAFE booklet on drowning prevention and water safety. The pretest was conducted before the intervention and posttest was done one month of intervention. The data collection tool was using a validated questionnaire on knowledge, attitude and practice for drowning prevention and water safety.

    Results: There were 719 respondents (response rate of 89.9%) participated at baseline and 53.7% at end line (after the intervention). Significant differences found in knowledge, attitudes and practice on drowning prevention and water safety for the intervention and control groups after the intervention (P<0.001). There was a significant difference in mean scores for knowledge and attitude before and after the intervention, whereas no significant findings noted for practices (P<0.001).

    Conclusion: Be SAFE booklet contributed to the increase in parents/guardian's knowledge and attitudes towards drowning prevention and water safety to prevent the risk of child drowning.

    MeSH terms: Attitude; Child; Drowning; Humans; Malaysia; Pamphlets; Parent-Child Relations; Parents; Surveys and Questionnaires; Schools; Control Groups
  15. Ting CY, Ho CC, Yee HJ
    Big Data, 2020 12;8(6):519-527.
    PMID: 33347366 DOI: 10.1089/big.2020.0028
    Recommending a retail business given a particular location of interest is nontrivial. Such a recommendation process requires careful study of demographics, trade area characteristics, sales performance, traffic, and environmental features. It is not only human effort taxing but often introduces inconsistency due to subjectivity in expert opinions. The process becomes more challenging when no sales data can be used to make a recommendation. As an attempt to overcome the challenges, this study used the machine learning approach that utilizes similarity measures to perform the recommendation. However, two challenges required careful attention when using the machine learning approach: (1) how to prepare a feature set that can commonly represent different types of retail business and (2) which similarity measure approach produces optimal recommendation accuracy? The data sets used in this study consist of points of interest, population, property, job type, and education level. Empirical studies were conducted to investigate (1) the overall accuracy of proposed similarity measure approaches to the retail business recommendation, and (2) whether the proposed approaches have a bias toward certain retail categories. In summary, the findings suggested that the proposed similarity-based techniques elicited an accuracy of above 70% and demonstrated higher accuracy when the recommendation was made within a set of similar retail businesses.
    MeSH terms: Machine Learning; Attention; Commerce; Demography; Educational Status; Expert Testimony; Humans; Taxes; Marketing
  16. Habib O, Mohd Sakri R, Ghazalli N, Chau DM, Ling KH, Abdullah S
    PLoS One, 2020;15(12):e0244386.
    PMID: 33347482 DOI: 10.1371/journal.pone.0244386
    CpG-free pDNA was reported to facilitate sustained transgene expression with minimal inflammation in vivo as compared to CpG-containing pDNA. However, the expression potential and impact of CpG-free pDNA in in vitro model have never been described. Hence, in this study, we analyzed the transgene expression profiles of CpG-free pDNA in vitro to determine the influence of CpG depletion from the transgene. We found that in contrast to the published in vivo studies, CpG-free pDNA expressed a significantly lower level of luciferase than CpG-rich pDNA in several human cell lines. By comparing novel CpG-free pDNA carrying CpG-free GFP (pZGFP: 0 CpG) to CpG-rich GFP (pRGFP: 60 CpGs), we further showed that the discrepancy was not influenced by external factors such as gene transfer agent, cell species, cell type, and cytotoxicity. Moreover, pZGFP exhibited reduced expression despite having equal gene dosage as pRGFP. Analysis of mRNA distribution revealed that the mRNA export of pZGFP and pRGFP was similar; however, the steady state mRNA level of pZGFP was significantly lower. Upon further investigation, we found that the CpG-free transgene in non-integrating CpG-free pDNA backbone acquired increased nucleosome enrichment as compared with CpG-rich transgene, which may explain the observed reduced level of steady state mRNA. Our findings suggest that nucleosome enrichment could regulate non-integrating CpG-free pDNA expression and has implications on pDNA design.
    MeSH terms: Animals; Cell Line; Humans; Luciferases/genetics; Nucleosomes/genetics*; Plasmids/genetics*; Species Specificity; Transfection; Gene Expression; CpG Islands*; Transgenes*; NIH 3T3 Cells; Green Fluorescent Proteins/genetics; Mice; HEK293 Cells; MCF-7 Cells
  17. Ng CM, Satvinder K, Koo HC, Yap RWK, Mukhtar F
    Matern Child Nutr, 2020 Dec;16 Suppl 3(Suppl 3):e13054.
    PMID: 33347728 DOI: 10.1111/mcn.13054
    The involvement of children in healthy meal preparation activities has emerged as a potential strategy to promote healthy eating behaviour among children. However, there is a lack of understanding of children's internal (psychosocial factors) and external factors (home food availability) that may support the practice of preparing healthy meals. This study aimed to determine children's psychosocial factors of healthy meal preparation within themselves and their external environment of home food availability as predictors for the practice of healthy meal preparation. Public schools (n = 8) from all three zones (Bangsar-Pudu, Keramat and Sentul) in Kuala Lumpur, Malaysia, were selected through stratified random sampling. Two hundred children aged 9-11 and their parents participated. Children's psychosocial factors towards healthy meal preparation and their home food availability were assessed through children and parents, respectively, using validated questionnaires. Majority of the schoolchildren (86.5%) had poor practice of healthy meal preparation. Increased attitude (r = 0.344, P < 0.001) and self-efficacy (r = 0.501, P < 0.001) of healthy meal preparation and the availability of fruits (r = 0.304, P < 0.001), vegetables (r = 0.243, P < 0.001) and healthful ready-to-eat foods (r = 0.227, P = 0.001) at home were positively correlated with the practice of preparing healthy meals. After adjusting for age, sex and monthly household income, increased self-efficacy (P < 0.001), availability of fruits (P = 0.01) and lower availability of less healthful ready-to-eat food (P = 0.01) were associated with better healthy meal preparation practices. Outcomes revealed that positive self-efficacy of healthy meal preparation, home food availability of fruits and less healthful alternatives were associated with the practice of healthy meal preparation and thus should be targeted in future health-promotion strategy.
    MeSH terms: Child; Cooking; Feeding Behavior; Female; Fruit; Humans; Male; Vegetables*; Meals*; Healthy Diet*
External Links