Browse publications by year: 2021

  1. Illyaaseen Z, Ngeow YF, Yap SF, Ng HF
    Malays J Pathol, 2021 Apr;43(1):55-61.
    PMID: 33903306
    Candida albicans is an important opportunistic fungal pathogen capable of causing fatal systemic infections in humans. Presently in Malaysia, there is little information available on the genetic diversity of this organism and trends in behavioural characteristics. In this project, three genotyping methods: 25S rDNA genotyping, Alternative Lengthening of Telomerase (ALT) sequence typing and Multi-Locus Sequence Typing (MLST) were applied to study the genetic diversity of strains from infected hospital in-patients and asymptomatic individuals in the community. The results showed that, with the 25S rDNA genotyping, as in other parts of the world, the most common genotype was type A which accounted for approximately 70% of the 111 isolates tested. Further typing with the ALT sequence showed type 3 to be the most common in the isolates tested. MLST analysis revealed many possibly novel sequence types, as well as a statistically significant association between pathogenicity and a group of closely related isolates, most of which were from hospital samples. Further work on genotypes associated with enhanced virulence will help to clarify the value of genotyping for clinical and epidemiological investigations.
    MeSH terms: Candida albicans; Candidiasis; DNA, Ribosomal; Genotype; Hospitals; Humans; Malaysia; Genetic Variation; Virulence; Telomerase; Multilocus Sequence Typing
  2. Jamali NS, Raja Sabudin RZA, Alauddin H, Ithnin A, Tumian NR, Jalil N, et al.
    Malays J Pathol, 2021 Apr;43(1):63-68.
    PMID: 33903307
    INTRODUCTION: The advent of BCR-ABL1-targeted therapy with the tyrosine kinase inhibitor (TKI), for example, imatinib and nilotinib, marked a turning point in the therapy of chronic myeloid leukaemia (CML). However, a substantial proportion of patients experience primary or secondary disease resistance to TKI. There are multifactorial causes contributing to the treatment failure of which BCR-ABL1 kinase domain mutation being the most common. Here, we describe a case of a CML patient with H396P mutation following treatment with nilotinib.

    CASE: A 60-year-old woman presented with abdominal discomfort and hyperleukocytosis. She was diagnosed as CML in the chronic phase with positive BCR-ABL1 transcripts. Due to the failure to obtain an optimal response with imatinib treatment, it was switched to nilotinib. She responded well to nilotinib initially and achieved complete haematological and cytogenetic responses, with undetectable BCR-ABL1 transcripts. However, in 4 years she developed molecular relapse. Mutation analysis which was done 70 months after commencement of nilotinib showed the presence of BCRABL1 kinase domain mutation with nucleotide substitution at position 1187 from Histidine(H) to Proline(P) (H396P). Currently, she is on nilotinib 400mg twice daily. Her latest molecular analysis showed the presence of residual BCR-ABL1 transcripts at 0.22%.

    DISCUSSION/CONCLUSION: This case illustrates the importance of BCR-ABL1 mutation analysis in CML patients with persistent BCR-ABL1 positivity in spite of treatment. Early detection and identification of the type of BCRABL1 mutation are important to guide appropriate treatment options as different mutation will have different sensitivity to TKI.

    MeSH terms: Imatinib Mesylate; Female; Histidine; Humans; Middle Aged; Mutation; Neoplasm Recurrence, Local; Nucleotides; Proline; Pyrimidines; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; Fusion Proteins, bcr-abl; Treatment Failure; Cytogenetic Analysis; Protein Kinase Inhibitors; Disease Resistance
  3. Che Jalil NA, Rama Chandran P, Samsudin AHZ, Yahya MM, Wan Abdul Rahman WF
    Malays J Pathol, 2021 Apr;43(1):69-73.
    PMID: 33903308
    Cancer metastasis to the thyroid gland from non-thyroid sites is a rare presentation in clinical practice. The most frequent primary cancers that metastasise to the thyroid are renal cell carcinoma, followed by colorectal, lung and breast. We report a case of a 64-year-old Malay lady who presented with anterior neck swelling 4 years after an initial diagnosis of uterine leiomyosarcoma. She had undergone a hysterectomy procedure four years ago. Fine needle aspiration cytology of the thyroid mass suggested undifferentiated thyroid carcinoma. After multi-disciplinary discussion, the patient underwent thyroidectomy and the final histopathological diagnosis was metastatic leiomyosarcoma of the thyroid. The diagnosis was aided by an immunohistochemistry panel of positive myogenic markers, negative epithelial markers as well as the previous medical history of uterine leiomyosarcoma. Metastatic leiomyosarcoma of the thyroid may mimic primary undifferentiated (anaplastic) thyroid carcinoma (UTC) with a sarcomatoid pattern, medullary thyroid carcinoma (MTC) with spindle cells morphology and spindle cell tumour with thymus-like differentiation (SETTLE). Hence, a multidisciplinary approach must be practised by pathologists, surgeons and radiologists to consider metastatic lesions of the thyroid gland, especially when a previous history of cancer exists or is suspected.
    MeSH terms: Breast Neoplasms; Carcinoma, Renal Cell; Cell Differentiation; Female; Humans; Hysterectomy; Immunohistochemistry; Kidney Neoplasms; Leiomyosarcoma; Lung; Malaysia; Middle Aged; Thyroid Neoplasms; Thyroidectomy; Colorectal Neoplasms; Carcinoma, Neuroendocrine; Biopsy, Fine-Needle; Thyroid Carcinoma, Anaplastic; Surgeons; Pathologists; Radiologists
  4. Mohtarrudin N, Bakrin IH, Ambrose D, Jo Lyn L, Mukhtar NSA
    Malays J Pathol, 2021 Apr;43(1):75-79.
    PMID: 33903309
    Cutaneous multiple myeloma (MM) is a rare disease. It can be primary or secondary in origin. The secondary type is further classified into specific and nonspecific types. The specific type is uncommon and is known as a secondary cutaneous plasmacytoma. We report a case of secondary cutaneous plasmacytoma in a 58-year-old man who had a history of plasma cell tumour of the lung and multiple myeloma. He achieved complete remission after the completion of chemotherapy and autologous stem cell transplant (ASCT). However, five months later, he developed multiple erythematous nodules on the whole body. Skin biopsy revealed diffuse neoplastic cells infiltrate in the reticular dermis with sparing of the upper papillary dermis and epidermis. The neoplastic cells were monotonous and homogenous with variable degrees of cytological atypia. Occasional cells showed distinctive plasma cell features. Plasma cell lineage was confirmed with CD138. The cells were immunoreactive to Kappa. Ki-67 was greater than 90%. They were non-immunoreactive to CD45, CD3, CD20, CD79 alpha and CK AE1/AE3. The findings were consistent with secondary cutaneous plasmacytoma. Our case illustrates that MM may present with nonspecific dermatological manifestations. As specific cutaneous involvement of MM is very uncommon; a high degree of clinical suspicion, detailed medical history and histopathological examination are required to arrive at an early diagnosis.
    MeSH terms: Biopsy; Epidermis; Humans; Lung; Male; Middle Aged; Multiple Myeloma; Plasma; Plasma Cells; Plasmacytoma; Skin; Cell Lineage; Ki-67 Antigen; Dermis; Stem Cell Transplantation; Rare Diseases; Early Diagnosis; Neoplasms, Plasma Cell
  5. Tan LJ, Othman MS, Hiu J, Wong KT, Lai SK
    Malays J Pathol, 2021 Apr;43(1):81-85.
    PMID: 33903310
    BACKGROUND: Small bowel volvulus is defined as the torsion of the small intestine, potentially leading to bowel obstruction, gangrene and perforation. It is a rare condition, especially in adults.

    CASE PRESENTATION: A 30-year-old man was retrieved from the jungle with severe weight loss and abdominal symptoms. He succumbed to death despite 22 days of intensive medical treatment. An autopsy revealed a ruptured gangrenous ileal volvulus with peritonitis and subdiaphragmatic abscess. Further laboratory analysis detected systemic Candida tropicalis and intestinal gramnegative bacterial sepsis, systemic Zika virus viremia, leptospirosis complicating rhabdomyolysis and disseminated intravascular coagulopathy, Type I Herpes Simplex virus infection of the tongue and upper gastrointestinal tract. The cause of death was the ruptured ileal volvulus, complicated with upper gastrointestinal bleeding due to Herpes simplex virus esophagitis in a malnourished patient with resolving leptospirosis and underlying Zika virus co-infection.

    CONCLUSION: Rare clinical scenarios of adult-onset intestinal volvulus with concomitant multiple infections precludes clinical diagnosis and early treatment, leading to devastating consequences of clinical outcome. The positive clinical and postmortem correlation is a good learning lesson in many disciplines of medicine and science.

    MeSH terms: Zika Virus; Adult; Autopsy; Cause of Death; Esophagitis; Gangrene; Gastrointestinal Hemorrhage; Herpes Simplex; Humans; Intestinal Obstruction; Intestine, Small; Laboratories; Leptospirosis; Male; Medicine; Rhabdomyolysis; Subphrenic Abscess; Tongue; Viremia; Weight Loss; Simplexvirus; Candida tropicalis; Upper Gastrointestinal Tract; Intestinal Volvulus; Coinfection
  6. Mohd Kasim NA, Al-Khateeb A, Chua YA, Sanusi AR, Mohd Nawawi H
    Malays J Pathol, 2021 Apr;43(1):87-93.
    PMID: 33903311
    Homozygous familial hypercholesterolaemia (HoFH) is a rare genetic disorder of lipoprotein metabolism mainly due to mutation of the low-density lipoprotein (LDL)-receptor gene (LDLR). It is a life-threatening disease that causes accelerated, multi-vessel atherosclerosis presented in early childhood. Pregnancy in HoFH may pose early coronary morbidity and mortality to both the foetus and mother. The combination of HoFH and pregnancy can be a fatal condition. While statins are very effective in lowering low-density lipoprotein cholesterol (LDL-C) levels, they are generally contraindicated during pregnancy, thus their use during pregnancy is uncommon. On the other hand, lipid apheresis (LA) has turned into an effective treatment to control cholesterol level amid pregnancy. However, the procedure is not widely available in our region. To date, there are scarcely documented case reports of HoFH in pregnancy in which the majority of them underwent LA to keep LDL-C at a low level. We report a rare case of successful pregnancy outcome of HoFH patient treated with lipid-lowering drugs including statin without LA therapy. Apart from that, we also discussed the genetic findings of the proband and all screened family members in which to the best of our knowledge, the first study using the whole-exome sequencing technique to identify the causative gene mutations for familial hypercholesterolaemia among the Malaysian population.
    MeSH terms: Hypolipidemic Agents; Blood Component Removal; Child; Child, Preschool; Pharmaceutical Preparations; Female; Fetus; Humans; Hyperlipoproteinemia Type II; Cholesterol, LDL; Morbidity; Mothers; Mutation; Pregnancy; Pregnancy Outcome; Hydroxymethylglutaryl-CoA Reductase Inhibitors; Atherosclerosis
  7. Wong YY, Alauddin H, Raja Sabudin RZA, Ithnin A, Jalil N, Abdul Latiff Z, et al.
    Malays J Pathol, 2021 Apr;43(1):95-100.
    PMID: 33903312
    The Siriraj I Gγ(Aγδβ)0-thalassaemia is a novel mutation involving a 118kb deletion of the β-globin gene cluster. It was first reported in 2012 in two unrelated families from the southern part of Thailand. The carriers in the heterozygous state are clinically asymptomatic. Nonetheless, its complex interaction with other β-thalassaemia could give rise to different clinical phenotypes, ranging from mild thalassaemia intermedia to thalassaemia major. We report here a case of a six-year-old Malay boy, presented with pallor, growth failure and hepatosplenomegaly. His haemoglobin at presentation was 9.2g/dL with a mean cell haemoglobin of 22.6pg and a mean cell volume of 69.9fl. His peripheral blood smear showed features of thalassaemia intermedia. Haemoglobin (Hb) analysis revealed markedly raised Hb F (83%), normal HbA2 levels and absent HbA. Deoxyribonucleic acid (DNA) analysis showed compound heterozygous IVS1-1 (G→T) β-globin gene mutation and Siriraj I Gγ(Aγδβ)0-deletion (genotype βIVS1-1/ β Siriraj I deletion). Both his father and elder sister are carriers of Siriraj I Gγ(Aγδβ)0-thalassaemia while his mother carries IVS1-1 (G→T) gene mutation. Clinically, the patient is transfusion dependent on six weekly regime. To the best of our knowledge, this is the first reported case in Malaysia involving unique Siriraj I Gγ(Aγδβ)0-thalassaemia and IVS1-1 (G→T) in a compound heterozygous state. In summary, detection of Siriraj I Gγ(Aγδβ)0-thalassaemia is essential as this deletion can lead to severe disease upon interaction with a β-thalassemia point mutation as demonstrated in our case. The establishment of effective carrier screening and genetic counselling is important to prevent its adverse consequences.
    MeSH terms: Aged; Child; DNA; Erythrocyte Indices; Fathers; Female; Multigene Family; Genetic Counseling; Heterozygote; Humans; Malaysia; Male; Mothers; Mutation; Pallor; Phenotype; Thailand; alpha-Thalassemia; beta-Thalassemia; Point Mutation; beta-Globins
  8. Kathiravelu Z, Thambiah SC, Mat Salleh MJ, Samsudin IN
    Malays J Pathol, 2021 Apr;43(1):101-107.
    PMID: 33903313
    INTRODUCTION: Haemolytic specimens are a frequent occurrence in clinical laboratories, and they interfere with the analysis of many tests.

    CASE REPORT: We describe here an unusual case of leptospirosis complicated by haemolytic anaemia in a 70-year-old man with established kidney failure. He presented with an abrupt onset of shortness of breath, flushing and erythematous rash after completing haemodialysis. The patient's biochemistry test samples were however rejected twice as they were grossly haemolysed. The integrated auto-verification alert system implemented in the hospital's laboratory information system alerted the staff of the possibility of in vivo haemolysis.

    DISCUSSION: The auto-verification alert system effectively distinguishes between in vitro and in vivo haemolysis and as such can be utilised as a diagnostic aid in patients with suspected intravascular haemolysis.

    MeSH terms: Aged; Anemia, Hemolytic; Body Fluids; Clinical Laboratory Information Systems; Dyspnea; Exanthema; Hematologic Tests; Renal Dialysis; Hemolysis; Hospitals; Humans; Laboratories; Leptospirosis; Male; Renal Insufficiency
  9. Chua TH, Takano A
    Malays J Pathol, 2021 04;43(1):121-125.
    PMID: 33903314
    No abstract available.
    MeSH terms: Brain
  10. Han HL, Kononenko VS
    Zootaxa, 2021 Apr 06;4951(2):zootaxa.4951.2.7.
    PMID: 33903406 DOI: 10.11646/zootaxa.4951.2.7
    Two new species of the genus Stenoloba Staudinger, 1892 (S. mediana, sp. n. and S. fuscobrunnea, sp. n.) are described from Cambodia and Laos respectively, and a new species of the genus Victrix Staudinger, 1879 (V. noloides, sp. n.) from China is described. Stenoloba chlorographa Kononenko Ronkay, 2001 is reported for the first time from China (Xizang), and new distributional data for recently described Stenoloba species from Malaysia are presented.
    MeSH terms: Animals; Animal Structures; China; Far East; Moths; Animal Distribution
  11. Figueroa A, Low MEY
    Zootaxa, 2021 Apr 01;4951(1):zootaxa.4951.1.10.
    PMID: 33903421 DOI: 10.11646/zootaxa.4951.1.10
    The snake genus Cylindrophis Wagler, 1828 belongs to the  monogeneric family Cylindrophiidae comprising 15 species distributed predominately throughout SE Asia, with one extralimital species occurring in Sri Lanka (Bernstein et al. 2020: 535). Cylindrophis lineatus is a rare species known from only eight museum specimens (discussed herein), and a photograph of one live individual from Kuching, Sarawak, East Malaysia (Stuebing et al. 2014: 63). Despite being originally described with Singapore as the type-locality (Blanford 1881: 217, 218), it is currently understood that C. lineatus is endemic to western Sarawak, East Malaysia (Stuebing et al. 2014: 63). Wallach et al. (2014: 204) stated that C. lineatus is also found in Kalimantan, but did not provide any references. This appears to have been followed by Bernstein et al. (2020: 537), who provide a map indicating C. lineatus occurrences in Kalimantan. The original description of Cylindrophis lineatus is conventionally cited as Blanford (1881: 217, 218, pl. 20). Herein, we demonstrate that the authorship and date of publication of this taxon should correctly be Cylindrophis lineatus Dennys, 1880b, and discuss that the type locality should be changed to "Borneo".
    MeSH terms: Animals; Lizards; Snakes/classification*; Animal Distribution
  12. Salini S, Kment P
    Zootaxa, 2021 Apr 14;4958(1):zootaxa.4958.1.31.
    PMID: 33903478 DOI: 10.11646/zootaxa.4958.1.31
    The genus Surenus Distant, 1901 (Hemiptera: Heteroptera: Pentatomoidea: Pentatomidae: Pentatominae: Halyini) is revisited and found to be a junior subjective synonym of the genus Agathocles Stål, 1876 (currently Pentatominae: Rolstoniellini). The genus Agathocles and its type species, Agathocles limbatus Stål, 1876, are redescribed. Lectotype of Surenus normalis Distant, 1901 (= Agathocles normalis (Distant, 1901) comb. nov.) is designated and the unknown male of the species is described. Agathocles yunnanensis Zhang Lin, 1984, syn. nov., is considered junior subjective synonym of A. limbatus. Two new species are described: Agathocles flavipes sp. nov. from India (Andhra Pradesh, Karnataka, Maharashtra, and Tamil Nadu) and A. joceliae sp. nov. from Malaysia (Kelantan, Perak). The new species differ from their congeners mainly by the morphology of mandibular plates, length of antennomeres I, IIa and IIb, body length, and structure of male genitalia. Agathocles dubius Distant, 1921 is transferred to the genus Caystrus Stål, 1861 (Pentatominae: Caystrini) based on examination of its holotype with the resulting new combination: Caystrus dubius (Distant, 1921), comb. nov. One new combination is proposed, Paramecocoris ruficornis (Fieber, 1851), comb. nov. (from preoccupied Paramecus Fieber, 1851), and its type locality is clarified as Tenasserim (south Myanmar). Gender agreement and authorship of the name Riazocoris niger Ahmad Afzal, 1977 in Ahmad et al. (1977: 161) are corrected and status of its name bearing type is clarified as lectotype. The following new distribution records are given: A. limbatus from Cambodia, China (Guangxi, Tibet), Laos and Thailand, A. normalis, Caystrus obscurus (Distant, 1901a) and Critheus lineatifrons Stål, 1869 from Laos, Amasenus corticalis Stål, 1863 from Cambodia, Indonesia (E Kalimantan), Laos, Myanmar and Thailand, and Rolstoniellus boutanicus (Dallas, 1849) from Vietnam. Based on characters of external morphology and genitalia, the genus Agathocles is compared with representatives of the genera Halys Fabricius, 1803 (Halyini), Caystrus (Caystrini), Laprius Stål, 1861 (Myrocheini), and Exithemus Distant, 1902 (currently in Rolstoniellini). As a result, the genus Agathocles is here transferred to the tribe Caystrini. The genus Kyrtalus Van Duzee, 1929 is tentatively placed in Myrocheini based on the presence of sulcate mesosternum and femora provided with teeth.
    MeSH terms: Animals; China; Hemiptera; India; Male; Animal Distribution
  13. Zhu W, Li S
    Zootaxa, 2021 Apr 16;4963(1):zootaxa.4963.1.5.
    PMID: 33903565 DOI: 10.11646/zootaxa.4963.1.5
    Six new species belonging to Belisana Thorell, 1898 are described from Southeast Asia: Belisana bachma sp. nov. (Vietnam; male, female), B. cucphuong sp. nov. (Vietnam; male, female), B. jaegeri sp. nov. (Malaysia; male, female), B. kachin sp. nov. (Myanmar; male, female), B. putao sp. nov. (Myanmar; male) and B. tarang sp. nov. (Indonesia; male, female). These new species bring the total number of Belisana to 143 species worldwide.
    MeSH terms: Animals; Asia, Southeastern; Myanmar; Female; Indonesia; Malaysia; Male; Spiders; Vietnam
  14. Eriksson K, Strimling P, Gelfand M, Wu J, Abernathy J, Akotia CS, et al.
    Nat Commun, 2021 Apr 26;12(1):2483.
    PMID: 33903599 DOI: 10.1038/s41467-021-22955-x
  15. A'ziz ANA, Minhat FI, Pan HJ, Shaari H, Saelan WNW, Azmi N, et al.
    Sci Rep, 2021 Apr 26;11(1):8890.
    PMID: 33903697 DOI: 10.1038/s41598-021-88404-3
    Pulau Tioman is a famous tourist island off Peninsular Malaysia with beautiful coral reefs. This study aims to assess the health of the coral reefs surrounding Pulau Tioman based on the application of the Foraminifera in Reef Assessment and Monitoring Index (FI). Ten sampling sites around Pulau Tioman were studied with a total of 30 samples. Eight orders, 41 families, 80 genera, and 161 species of benthic foraminifera were identified. The agglutinated type of foraminifera constituted 2-8% of the total assemblages. Calcareous hyaline and porcelaneous groups represented 79% and 19% of the total assemblages, respectively. Symbiont-bearing taxa were the most common foraminifera. The results indicate that most of the sampling sites are conducive for coral reef growth with good recoverability from future stress to the ecosystem. However, several areas with higher coastal development and tourism have reduced water and sediment quality. Therefore, the limit on the number of visitors and tourists should be revised to enable coral growth and health. The FI values in this study showed a positive correlation with good water qualities and a negative correlation with organic matter enrichment. The FI is a good measure to assess the health of a coral reef and can be applied to other reef ecosystems around Malaysia.
    MeSH terms: Animals; Biological Phenomena; Humans; Hyalin; Malaysia; Water; Ecosystem; Anthozoa; Foraminifera; Coral Reefs; Water Quality; Islands
  16. Ghani ASA, Rahim AFA, Yusoff MSB, Hadie SNH
    Med Sci Educ, 2021 Jun;31(3):1199-1211.
    PMID: 33903829 DOI: 10.1007/s40670-021-01292-0
    Problem-based learning (PBL) emphasizes learning behavior that leads to critical thinking, problem-solving, communication, and collaborative skills in preparing students for a professional medical career. However, learning behavior that develops these skills has not been systematically described. This review aimed to unearth the elements of effective learning behavior in a PBL context, using the protocol by Arksey and O'Malley. The protocol identified the research question, selected relevant studies, charted and collected data, and collated, summarized, and reported results. We discovered three categories of elements-intrinsic empowerment, entrustment, and functional skills-proven effective in the achievement of learning outcomes in PBL.
  17. Mai CW, Shu Y, Cheong SK, Chua CW
    Sheng Li Xue Bao, 2021 Apr 25;73(2):181-196.
    PMID: 33903880
    Organoids are self-organized cellular clusters in three-dimensional culture, which can be derived from a single stem cell, progenitor or cell clusters of different lineages resembling in vivo tissue architecture of an organ. In the recent years, organoids technology has contributed to the revolutionary changes in stem cell and cancer fields. In this review, we have briefly overviewed the emerging landscape of prostate organoid technology (POT) in prostate research. In addition, we have also summarized the potential application of POT in the understanding of prostate stem cell and cancer biology and the discovery of novel therapeutic strategies for prostate cancer. Lastly, we have critically discussed key challenges that lie in the current state of POT and provided a future perspective on the second-generation of POT, which should better recapitulate cellular behaviors and drug responses of prostate cancer patients.
    MeSH terms: Gold; Humans; Male; Neoplasms*; Organoids*; Prostate; Stem Cells; Technology
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