Browse publications by year: 2024

  1. Cheah KJ, Abdul Manaf Z, Fitri Mat Ludin A, Razalli NH, Mohd Mokhtar N, Md Ali SH
    JMIR Mhealth Uhealth, 2024 Mar 12;12:e49055.
    PMID: 38532298 DOI: 10.2196/49055
    BACKGROUND: The success of mobile apps in improving the lifestyle of patients with noncommunicable diseases through self-management interventions is contingent upon the emerging growth in this field. While users of mobile health (mHealth) apps continue to grow in number, little is known about the quality of available apps that provide self-management for common noncommunicable diseases such as diabetes, hypertension, and obesity.

    OBJECTIVE: We aimed to investigate the availability, characteristics, and quality of mHealth apps for common noncommunicable disease health management that included dietary aspects (based on the developer's description), as well as their features for promoting health outcomes and self-monitoring.

    METHODS: A systematic search of English-language apps on the Google Play Store (Google LLC) and Apple App Store (Apple Inc) was conducted between August 7, 2022, and September 13, 2022. The search terms used included weight management, obesity, diabetes, hypertension, cardiovascular diseases, stroke, and diet. The selected mHealth apps' titles and content were screened based on the description that was provided. Apps that were not designed with self-management features were excluded. We analyzed the mHealth apps by category and whether they involved health care professionals, were based on scientific testing, and had self-monitoring features. A validated and multidimensional tool, the Mobile App Rating Scale (MARS), was used to evaluate each mHealth app's quality based on a 5-point Likert scale from 1 (inadequate) to 5 (excellent).

    RESULTS: Overall, 42 apps were identified. Diabetes-specific mHealth apps accounted for 7% (n=3) of the market, hypertension apps for 12% (n=5), and general noncommunicable disease management apps for 21% (n=9). About 38% (n=16) of the apps were for managing chronic diseases, while 74% (n=31) were for weight management. Self-management features such as weight tracking, BMI calculators, diet tracking, and fluid intake tracking were seen in 86% (n=36) of the apps. Most mHealth apps (n=37, 88%) did not indicate whether there was involvement of health professionals in app development. Additionally, none of the apps reported scientific evidence demonstrating their efficacy in managing health. The overall mean MARS score was 3.2 of 5, with a range of 2.0 to 4.1. Functionality was the best-rated category (mean score 3.9, SD 0.5), followed by aesthetics (mean score 3.2, SD 0.9), information (mean score 3.1, SD 0.7), and engagement (mean score 2.9, SD 0.6).

    CONCLUSIONS: The quality of mHealth apps for managing chronic diseases was heterogeneous, with roughly half of them falling short of acceptable standards for both quality and content. The majority of apps contained scant information about scientific evidence and the developer's history. To increase user confidence and accomplish desired health outcomes, mHealth apps should be optimized with the help of health care professionals. Future studies on mHealth content analysis should focus on other diseases as well.

  2. Goh SF, Wong SB, Robinson S, Tang MM
    Exp Dermatol, 2024 Mar;33(3):e15060.
    PMID: 38532576 DOI: 10.1111/exd.15060
    Psoriatic arthritis (PsA) is a major comorbidity of psoriasis and may lead to irreversible joint damage and disability. This study aims to describe the clinical profile, treatment and quality of life (QoL) of patients with PsA in Malaysia. This is a multicentre retrospective cross-sectional study of psoriasis patients who were notified to the Malaysian Psoriasis Registry (MPR) from January 2007 to December 2018. Of 21 735 psoriasis patients, 2756 (12.7%) had PsA. The male to female ratio was 1:1. The mean age of psoriasis onset for PsA patients was 34.73 ± 14.44 years. They had a higher rate of family history of psoriasis (26% vs. 22.4%, p 10% and/or Dermatology Life Quality Index (DLQI) >10)]. Most had oligo-/monoarthropathy (40.3%), followed by distal interphalangeal arthropathy (31.3%), symmetrical polyarthropathy (28.3%), spondylitis/sacroiliitis (8.2%) and arthritis mutilans (3.2%). Nearly 40% of PsA patients received systemic treatment, but only 1.6% received biologic agents. QoL was more significantly affected in PsA than in non-PsA patients (mean DLQI 10.12 ± 7.16 vs. 9.52 ± 6.67, p 
    MeSH terms: Adult; Cross-Sectional Studies; Female; Humans; Malaysia; Male; Middle Aged; Quality of Life; Retrospective Studies; Young Adult
  3. Lin GSS, Hashim H
    J Dent Educ, 2024 Jul;88(7):949-956.
    PMID: 38532665 DOI: 10.1002/jdd.13526
    PURPOSE: The present study aims to evaluate students' perceptions of different types of written assessment within the context of dental materials science and to identify how various learning styles exhibited by dental students, as well as gender and ethnicity, may influence their perceptions of these assessment methods.

    METHODS: The study was conducted among second-year dental students in a dental materials science class, during which students participated in a mock assessment that included extended matching questions (EMQs) and single correct answer (SCA) questions. An online questionnaire comprising three sections: demographic information, Honey and Mumford's Learning Styles Questionnaire, and 15 closed-ended questionnaire items employing a 5-point Likert scale were administered to assess students' perceptions of the assessment methods. Statistical analyses were conducted using the Kruskal-Wallis test, Mann-Whitney U test, and a post-hoc Bonferroni test.

    RESULTS: All 70 students participated in the study with the majority being female, Chinese, and activist learners. Median total scores among various learning styles, gender, and ethnicity did not exhibit significant differences. Analysis of individual questionnaire items revealed mixed perceptions regarding EMQs. Reflectors generally held more positive perceptions of EMQs, while theorists showed the lowest total median scores toward EMQs. Most students did not prefer EMQs over SCAs, and some students expressed confusion about EMQs, especially those with a theorist learning style.

    CONCLUSION: Although students generally showed good perceptions towards these assessment methods, further research is needed to better understand the interplay of learning styles, assessment preferences, and educational outcomes in dental education.

    MeSH terms: Female; Humans; Learning; Male; Surveys and Questionnaires; Young Adult
  4. Xu Y, Razak RRA, Xiang M
    Heliyon, 2024 Mar 30;10(6):e28331.
    PMID: 38533043 DOI: 10.1016/j.heliyon.2024.e28331
    BACKGROUND: In the contemporary academic milieu, an array of erudite investigations has meticulously delved into the ramifications of social media paradigms on the nuances of modern societal interactions. Predominantly, these scholarly endeavors have harnessed quantitative methodologies to discern both the advantageous and detrimental implications of social media. Notwithstanding these extensive analyses, there appears to be a conspicuous lacuna in the literature about the subjective repercussions of social media on the well-being and contentment of tertiary education students. In light of this gap, the present qualitative exploration seeks to elucidate the perceptions of Chinese collegiate individuals vis-à-vis the influence of social media platforms on their experiential happiness.

    METHOD: Guided by interpretative phenomenological analysis (Jonathan et al., 2022) [1], we conducted 3 semi-structured interviews with 5 university students.

    RESULT: Using an interpretive phenomenology analysis approach four themes. They are (1) The Paradox of Temperance and Indulgence, (2) Identity Construction and Presentation, (3) Social Support and Connection, and (4) Social Comparison and Self-Evaluation.

    DISCUSSION: Findings suggest that the individual well-being of college students may be affected by ambivalence between moderate and indulgent use of social media, social media interactions to maintain and enhance personal identity, and comparisons between individuals and certain online groups. Therefore, the government, higher education institutions, and college students should work together to build a safe and happy university life.

  5. Deng N, Soh KG, Abdullah BB, Huang D
    Heliyon, 2024 Mar 30;10(6):e28051.
    PMID: 38533062 DOI: 10.1016/j.heliyon.2024.e28051
    Skill-related physical fitness significantly correlates with sports performance. Plyometric training (PT) is an effective method for improving physical fitness in athletes. However, its impact on skill-related physical fitness in badminton players remains uncertain. Therefore, this systematic review and meta-analysis aimed to evaluate the effectiveness of PT on skill-related physical fitness in badminton players. Five electronic databases (Web of Science, PubMed, SCOPUS, MEDLINE, and SPORTSDiscus) were searched until February 2024. A PICOS approach was used to identify inclusion criteria, (1) healthy badminton players, (2) a PT program, (3) an active control group, (4) a measure of skill-related physical fitness before and after PT, and (5) randomized controlled studies. The PEDro scale was used to assess the methodological quality of PT studies, while the level of evidence certainty was determined through the GRADE framework. The calculation of effect sizes (ESs) was based on mean values and standard deviations, and heterogeneity was measured with the I2 statistic. The extended Egger's test was employed to check for publication bias. Eleven studies comprising 445 badminton players were eligible for inclusion. The analysis revealed significant small-to-moderate effects of PT on power (ES = 0.60, p 
  6. Hanafiah A, Abd Aziz SNA, Md Nesran ZN, Wezen XC, Ahmad MF
    Heliyon, 2024 Mar 30;10(6):e28128.
    PMID: 38533069 DOI: 10.1016/j.heliyon.2024.e28128
    The impact of H. pylori resistance on patient's treatment failure is a major concern. Therefore, the development of novel or alternative therapies for H. pylori is urgently needed. The purpose of this study was to investigate the molecular interactions of various antimicrobial peptides (AMPs) to H. pylori proteins. We performed the peptide-protein molecular docking using HADDOCK 2.4 webserver. Fourteen AMPs were tested for their binding efficacy against four H. pylori proteins. Simulation of the peptide-protein complex was performed using molecular dynamic software package AMBER20. From molecular docking analysis, five peptides (LL-37, Tilapia piscidin 4, napin, snakin-1 and EcAMP1) showed strong binding interactions against H. pylori proteins. The strongest binding affinity was observed in the interactions between Snakin-1 and PBP2, TP4 and type I HopQ and EcAMP1 and type I HopQ with -11.1, -13.6 and -13.8 kcal/mol, respectively. The dynamic simulation was performed for two complexes (snakin1-PBP2 and EcAMP1-HopQ). Results of the dynamics simulation showed that EcAMP1 had stable interaction and binding to type I HopQ protein without significant structural changes. In conclusion, both results of docking and simulation showed that EcAMP1 might be useful as a potential therapeutic agent for H. pylori treatment. This molecular approach provides deep understanding of the interaction insights between AMPs and H. pylori proteins. It paves the way for the development of novel anti-H. pylori using antimicrobial peptides.
  7. Feng W, Wang F, Han Y, Li G
    Heliyon, 2024 Mar 30;10(6):e27544.
    PMID: 38533080 DOI: 10.1016/j.heliyon.2024.e27544
    PURPOSE: The purpose of the study was to examine the impact of core strength training on the dynamic balance, agility, and dribbling ability of adolescent basketball players.

    METHODS: A randomized controlled between-subjects design was employed. Forty-four male adolescent basketball players (aged 14.41 ± 3.22 years) were randomly divided into two groups: the core strength training (CST) group and the conventional training (CT) group. The CST program included 1-h sessions, three times/week for 12 weeks. In contrast, the CT group provided a thorough physical training program that targeted general conditioning rather than focusing solely on core strength. Three measurements were used to evaluate performance in players: the Star Excursion Balance Test, the Illinois Agility Test, and the Dribbling Test conducted at T0 (week 0), T1 (week 6), and T2 (week 12), respectively.

    RESULTS: Compared to the CT group, the CST group showed a greater improvement (p  0.05).

    CONCLUSION: The 12-week CST program significantly improved dynamic balance, agility, and dribbling skills in adolescent basketball players, demonstrating its potential as a valuable training component. Future research should explore CST's impact on other sport-specific elements and its applicability to female players.

  8. Lam S, Hoffmann V, Bett B, Fèvre EM, Moodley A, Mohan CV, et al.
    One Health, 2024 Jun;18:100710.
    PMID: 38533195 DOI: 10.1016/j.onehlt.2024.100710
    Adopting One Health approaches is key for addressing interconnected health challenges. Yet, how to best put One Health into practice in research-for-development initiatives aiming to 'deliver impacts' remains unclear. Drawing on the CGIAR Initiative on One Health - a global initiative to address zoonotic diseases, antimicrobial resistance, and food and water safety - we reflect on challenges during program conception and implementation, prompting us to suggest improvements in multisectoral collaboration, coordination, and communication. Our approach involves conducting a researcher-centered process evaluation, comprising individual interviews that are subsequently thematically analyzed and synthesized. The key takeaway is that limited time for planning processes and short program timelines compared to envisioned development impacts may impede research-for-development efforts. Yet, collaborative work can be successful when adequate time and resources are allocated for planning with minimal disruption throughout implementation. Additionally, due to the multifaceted nature of One Health initiatives, it is important to pay attention to co-benefits and trade-offs, where taking action in one aspect may yield advantages and disadvantages in another, aiding to identify sustainable One Health development pathways. Forming close partnerships with national governments and local stakeholders is essential not only to promote sustainability but also to ensure local relevance, enhancing the potential for meaningful impact. Finally, regularly assessing progress toward development goals is critical as development stands as an overarching objective.
  9. Saif-Ali R, Al-Hamodi Z, Salem SD, Al-Habori M, Al-Dubai SA, Ismail IS
    Indian J Endocrinol Metab, 2024;28(1):55-59.
    PMID: 38533286 DOI: 10.4103/ijem.ijem_209_23
    INTRODUCTION: Type 2 diabetes (T2D) candidate genes, protein tyrosine phosphatase receptor type D (PTPRD), and serine racemase (SRR) were suggested by a genome-wide association study (GWAS) in the Chinese population. Association studies have been replicated among East Asian populations. The association of PTPRD and SRR genetic variants with T2D in Southeast Asian populations still needs to be studied. This study aimed to investigate the association of PTPRD and SSR genetic variants with T2D in Malaysian Indian subjects.

    METHODS: The single nucleotide polymorphisms (SNPs) of PTPRD (rs649891 and rs17584499) and SRR (rs4523957, rs391300, and rs8081273) were genotyped in 397 T2D and 285 normal Malaysian Indian subjects.

    RESULTS: The homozygous dominant genotype of rs17584499 is frequent in diabetic patients (56.5%) compared to normal subjects (47.3%). In contrast, the homozygous recessive genotype of rs8081273 is more frequent among normal subjects (12.5%) than diabetic patients (5.6%). The dominant genetic model showed that PTPRD rs17584499 (CC) is a risk factor for T2D (OR = 1.42, P = 0.029), whereas the recessive genetic model showed that SRS SNP rs8081273 was protective for T2D (OR = 0.42, P = 0.003).

    CONCLUSION: This study confirmed the association of PTPRD rs17584499 genetic variations with T2D in Malaysian Indians. While the SRR rs8081273 (TT) genotype showed protection against T2D, more investigation in different populations is required to confirm this protection.

  10. Chandran S, Louis NE, Amran SI, Ab Latif N, Hamza MA, Alonazi M
    Biomedicine (Taipei), 2024;14(1):47-59.
    PMID: 38533298 DOI: 10.37796/2211-8039.1431
    An inherent genetic enzyme disorder in humans, known as glucose-6-phosphate dehydrogenase (G6PD) deficiency, arises due to specific mutations. While the prevailing approach for investigating G6PD variants involves biochemical analysis, the intricate structural details remain limited, impeding a comprehensive understanding of how different G6PD variants of varying classes impact their functionality. This study 22 examined the dynamic properties of G6PD wild types and six G6PD variants from 23 different classes using molecular dynamic simulation (MDS). The wild-type and variant 24 G6PD structures unveil high fluctuations within the amino acid range of 274-515, the structural NADP+ binding site, pivotal for enzyme dimerization. Specifically, two variants, G6PDZacatecas (R257L) and G6PDDurham (K238R), demonstrate compromised structural stability at the dimer interface, attributable to the disruption of a salt bridge involving Glu 206 and Lys 407, along with the disturbance of hydrogen bonds formed by Asp 421 at the βN-βN sheets. Consequently, this impairment cascades to affect the binding affinity of crucial interactions, such as Lys 171-Glucose-6-Phosphate (G6P) and Lys 171-catalytic NADP+, leading to diminished enzyme activity. This study underscores the utility of computational in silico techniques in predicting the structural alterations and flexibility of G6PD variants. This insight holds promise for guiding future endeavors in drug development targeted at mitigating the impacts of G6PD deficiency.
  11. Khalid K, Lim HX, Anwar A, Tan SH, Hwang JS, Ong SK, et al.
    AAPS PharmSciTech, 2024 Mar 12;25(3):60.
    PMID: 38472523 DOI: 10.1208/s12249-024-02778-x
    The protective efficacies of current licensed vaccines against COVID-19 have significantly reduced as a result of SARS-CoV-2 variants of concern (VOCs) which carried multiple mutations in the Spike (S) protein. Considering that these vaccines were developed based on the S protein of the original SARS-CoV-2 Wuhan strain, we designed a recombinant plasmid DNA vaccine based on highly conserved and immunogenic B and T cell epitopes against SARS-CoV-2 Wuhan strain and the Omicron VOC. Literature mining and bioinformatics were used to identify 6 immunogenic peptides from conserved regions of the SARS-CoV-2 S and membrane (M) proteins. Nucleotide sequences encoding these peptides representing highly conserved B and T cell epitopes were cloned into a pVAX1 vector to form the pVAX1/S2-6EHGFP recombinant DNA plasmid vaccine. The DNA vaccine was intranasally or intramuscularly administered to BALB/c mice and evaluations of humoral and cellular immune responses were performed. The intramuscular administration of pVAX1/S2-6EHGFP was associated with a significantly higher percentage of CD8+ T cells expressing IFN-γ when compared with the empty vector and PBS controls. Intramuscular or intranasal administrations of pVAX1/S2-6EHGFP resulted in robust IgG antibody responses. Sera from mice intramuscularly immunized with pVAX1/S2-6EHGFP were found to elicit neutralizing antibodies capable of SARS-CoV-2 Omicron variant with the ACE2 cell surface receptor. This study demonstrated that the DNA vaccine construct encoding highly conserved immunogenic B and T cell epitopes was capable of eliciting potent humoral and cellular immune responses in mice.
    MeSH terms: Animals; Antibodies, Viral; Humans; Mice, Inbred BALB C; Peptides; CD8-Positive T-Lymphocytes; Epitopes, T-Lymphocyte; Vaccines, DNA*; Mice
  12. Ali A, Yasin MNM, Adam I, Ismail AM, Jack SP, Alghaihab A, et al.
    Heliyon, 2024 Mar 30;10(6):e27782.
    PMID: 38524620 DOI: 10.1016/j.heliyon.2024.e27782
    An improved mutual coupling compensation in circularly polarized (CP) multi-input multi-output (MIMO) dielectric resonator antenna (DRA) is presented in this paper. Using trimming approach, the mutual coupling (MC) between closely spaced DRA units at 0.3λ has been significantly reduced while axial ratio performance has been maintained. Mutual coupling reduction is obtained by trimming the DRA to ensure low mutual coupling below -20dB. The exclusive features of the proposed MIMO DRA include wide impedance matching bandwidth (BW), triple band circular polarization, and suppressed MC between the radiating elements. The impedance bandwidth matches perfectly with a triple band's 3 dB axial ratio (AR). It is designed with characteristic mode analysis with good agreement of the measurement that has been obtained. Using the probe feed method, the DRA and patch strip are coupled together to allow bandwidth widening of the pro-posed DRA. An impedance bandwidth of 34% at a lower frequency to around 2% at a higher frequency was achieved in all resonance frequencies. Thus, we refer to our newly designed DRA as a proposed method for effectively reducing the mutual coupling between DRAs. Additionally, the 3 dB AR bandwidth matched at 3.3 GHz, 4.6 GHz, and 6.3 GHz with a percentage of 11.66%, 3.04%, and 2.22% obtained at the three different frequencies. Note that the proposed DRA exhibits low mutual coupling (below -20 dB) at the targeted frequencies, which is suitable for better signal reception for MIMO applications. By computing, the metrics envelop correlation coefficient, diversity gain, channel capacity loss, and total active reflection coefficient, the MIMO performance of the proposed antenna is verified. The experiments show a close result between simulated and computed validation of the proposed DRA.
  13. Wong JHD, Ismail WH
    Phys Eng Sci Med, 2024 Mar 25.
    PMID: 38526646 DOI: 10.1007/s13246-024-01407-y
    The use of Al2O3:C-based optically stimulated luminescent dosimeters (OSLDs) in diagnostic X-ray is a challenge because of their energy dependence (ED) and variability of element sensitivity factors (ESFs). This study aims to develop a method to determine ED and ESFs of Landauer nanoDot™ OSLDs for clinical X-ray and investigate the uncertainties associated with ESF and ED correction factors. An area of 2 × 2 cm2 at the central axis of the X-ray field was used to establish the ESFs. A total of 80 OSLDs were categorized into "controlled" (n = 40) and "less-controlled" groups (n = 40). The ESFs of the OSLDs were determined using an 80 kVp X-ray beam quality in free-air geometry. The OSLDs were cross-calibrated with an ion chamber to establish the average calibration coefficient and ESFs. The OSLDs were then irradiated at tube potentials ranging from 50 to 150 kVp to determine their ED. The uniformity of the X-ray field was ± 1.5% at 100 cm source-to-surface distance. The batch homogeneities of user-defined ESFs were 2.4% and 8.7% for controlled and less-controlled OSLDs, respectively. The ED of OSLDs ranged from 1.125 to 0.812 as tube potential increased from 50 kVp to 150 kVp. The total uncertainty of OSLDs, without ED correction, could be as high as 16%. After applying ESF and ED correction, the total uncertainties were reduced to 6.3% in controlled OLSDs and 11.6% in less-controlled ones. OSLDs corrected with user-defined ESF and ED can reduce the uncertainty of dose measurements in diagnostic X-rays, particularly in managing less-controlled OSLDs.
  14. Zhong W, Osther P, Pearle M, Choong S, Mazzon G, Zhu W, et al.
    World J Urol, 2024 Mar 25;42(1):189.
    PMID: 38526675 DOI: 10.1007/s00345-024-04816-6
    BACKGROUND: The stone burden based management strategy reported in the guidelines published by different associations is well known for a long time. Staghorn calculi, representing the largest burden and most complex stones, is one of the most challenging cases to practicing urologists in clinical practice. The International Alliance of Urolithiasis (IAU) has released a series of guidelines on the management of urolithiasis.

    PURPOSE: To develop a series of recommendations for the contemporary management management of staghorn calculi and to provide a clinical framework for urologists treating patients with these complex stones.

    METHODS: A comprehensive literature search for articles published in English between 01/01/1976 and 31/12/2022 in the PubMed, OVID, Embase and Medline database is performed. A series of recommendations are developed and individually graded following the review of literature and panel discussion.

    RESULTS: The definition, pathogenesis, pathophysiology, preoperative evaluation, intraoperative treatment strategies and procedural advice, early postoperative management, follow up and prevention of stone recurrence are summarized in the present document.

    CONCLUSION: A series of recommendations regarding the management of staghorn calculi, along with related commentary and supporting documentation offered in the present guideline is intended to provide a clinical framework for the practicing urologists in the management of staghorn calculi.

    MeSH terms: Humans
  15. Sahu JN, Dhaouadi F, Sellaoui L, Khor LX, Lee SY, Daud WMAW, et al.
    Environ Sci Pollut Res Int, 2024 Apr;31(19):27980-27987.
    PMID: 38526713 DOI: 10.1007/s11356-024-33002-9
    The adsorption of ammonium from water was studied on an activated carbon obtained using raw oil palm shell and activated with acetic acid. The performance of this adsorbent was tested at different operating conditions including the solution pH, adsorbent dosage, and initial ammonium concentration. Kinetic and equilibrium studies were carried out, and their results were analyzed with different models. For the adsorption kinetics, the pseudo-first order equation was the best model to correlate this system. Calculated adsorption rate constants ranged from 0.071 to 0.074 g/mg min. The ammonium removal was 70-80% at pH 6-8, and it was significantly affected by electrostatic interaction forces. Ammonium removal (%) increased with the adsorbent dosage, and neutral pH condition favored the adsorption of this pollutant. The best ammonium adsorption conditions were identified with a response surface methodology model where the maximum removal was 91.49% with 2.27 g/L of adsorbent at pH 8.11 for an initial ammonium concentration of 36.90 mg/L. The application of a physical monolayer model developed by statistical physics theory indicated that the removal mechanism of ammonium was multi-ionic and involved physical interactions with adsorption energy of 29 kJ/mol. This activated carbon treated with acetic acid is promising to depollute aqueous solutions containing ammonium.
    MeSH terms: Adsorption; Charcoal/chemistry; Hydrogen-Ion Concentration; Kinetics; Water Purification/methods; Arecaceae/chemistry
  16. Song BPC, Lai JY, Lim TS
    Methods Mol Biol, 2024;2793:21-40.
    PMID: 38526721 DOI: 10.1007/978-1-0716-3798-2_2
    Phage display antibody libraries have been successfully used as the essential tool to produce monoclonal antibodies against a plethora of targets ranging from diseases to native biologically important proteins as well as small molecules. It is well documented that diverse antibody genes are the major genetic source for the construction of a high-quality antibody library and selection of high-affinity antibodies. Naïve antibody libraries are derived using the IgM repertoire of healthy donors obtained from B-cells isolated from human peripheral blood mononuclear cell (PBMC). Single-chain fragment variable (scFv) is a routinely used format due to its smaller size and preference for phage display. The process involves the use of a two-step cloning method for library construction. The protocol also covers the biopanning process for target positive clone selection.
    MeSH terms: Antibodies, Monoclonal; Humans; Leukocytes, Mononuclear; Peptide Library; Cell Surface Display Techniques
  17. Wong HY, Rajasuriar R, Wong PL, Lee YK
    AIDS Care, 2024 Mar 25.
    PMID: 38526987 DOI: 10.1080/09540121.2024.2331220
    The proportion of new HIV diagnoses among older adults aged ≥50 years continues to rise. Older adults are at higher risk of late diagnosis which is associated with higher treatment complexity and poorer health outcomes. Few studies in the Asia-Pacific region have explored factors contributing to late presentation and diagnosis in this population. Thus, our study aimed to explore factors influencing late HIV diagnosis among older adults ≥50 years in Malaysia. We conducted in-depth interviews with 16 older adults newly diagnosed with HIV (OPLWH) and focus group discussions with seven healthcare providers (HCPs) from different specialties in an academic tertiary hospital in Malaysia. All sessions were audio-recorded, transcribed verbatim and analysed thematically. Three main themes related to late diagnosis among OPLWH emerged: (1) challenge in recognizing HIV symptoms among older persons, (2) older persons and HCPs having low index of suspicion of HIV and (3) poor acceptance of HIV testing among older persons due to perceived personal and social identities. HCPs often missed HIV symptoms and these collectively culminated in OPLWH experiencing complex diagnostic journeys resulting in late HIV diagnosis. To reduce delays in HIV diagnosis, strategies are needed to improve HIV knowledge and risk perception among both older adults and HCPs.
  18. Wang P, Soh KL, Japar SB, Khazaai HB, Liao J, Ying Y, et al.
    PLoS One, 2024;19(3):e0300067.
    PMID: 38527072 DOI: 10.1371/journal.pone.0300067
    INTRODUCTION: There is currently no gold standard or specific nutritional assessment tool to assess malnutrition in patients with nasopharyngeal carcinoma (NPC). Our study aims to develop a new nutritional assessment tool for NPC patients.

    METHODS AND ANALYSIS: NPC patients will be required to complete a risk factor questionnaire after obtaining their informed consent. The risk factor questionnaire will be used to collect potential risk factors for malnutrition. Univariate and multivariate logistic regression analyses will be used to identify risk factors for malnutrition. A new nutritional assessment tool will be developed based on risk factors. The new tool's performance will be assessed by calibration and discrimination. The bootstrapping will be used for internal validation of the new tool. In addition, external validation will be performed by recruiting NPC patients from another hospital.

    DISCUSSION: If the new tool is validated to be effective, it will potentially save medical staff time in assessing malnutrition and improve their work efficiency. Additionally, it may reduce the incidence of malnutrition and its adverse consequences.

    STRENGTHS AND LIMITATIONS OF THIS STUDY: The study will comprehensively analyze demographic data, disease status, physical examination, and blood sampling to identify risk factors for malnutrition. Furthermore, the new tool will be systematically evaluated, and validated to determine their effectiveness. However, the restricted geographical range may limit the generalizability of the results to other ethnicities. Additionally, the study does not analyze subjective indicators such as psychology.

    ETHICS AND DISSEMINATION: The ethical approval was granted by the Ethical Committee of the First Affiliated Hospital of Guangxi Medical University (NO. 2022-KT-GUI WEI-005) and the Second Affiliated Hospital of Guangxi Medical University (NO. 2022-KY-0752).

    CLINICAL TRIAL REGISTRATION NUMBER: ChiCTR2300071550.

    MeSH terms: China/epidemiology; Humans; Nutritional Status; Nutrition Assessment
  19. Salim SA, Baharudin NH, Ibrahim NS, Abd Ghani Z, Ismail MN
    PMID: 38527182 DOI: 10.1080/19440049.2024.2329614
    Rice is one of the crops cultivated in Malaysia, and it is the main diet for most of the population. In this study, dispersive liquid-liquid micro-extraction (DLLME) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to develop, optimise and validate a reliable, easy-to-use and quick approach to detect aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1) and aflatoxin G2 (AFG2). The extraction recoveries in DLLME were enhanced by the addition of 5% salt, utilising chloroform as the extraction solvent and acetonitrile as the dispersive solvent. The DLLME parameters - the extraction solvent volume, salt concentration and dispersive solvent volume were optimised with Box-Behnken design (BBD) and response surface methodology (RSM). Under optimised experimental conditions, excellent linearity was obtained with a limit of detection (LOD) ranging from 0.125 to 0.25 ng g-1, a limit of quantitation (LOQ) ranging from 0.25 to 0.3 ng g-1 and a correlation value (R2) of 0.990. The matrix effects were between -11.1% and 19.9%, and recoveries ranged from 87.4% to 117.3%. The optimised and validated method was used effectively to assess aflatoxins contamination in 20 commercial rice samples collected from local supermarkets in Penang, Malaysia. AFB1 was detected at 0.41-0.43 ng g-1 in two rice samples, below the regulatory limit.
    MeSH terms: Malaysia; Tandem Mass Spectrometry; Liquid Phase Microextraction*
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