Browse publications by year: 2024

  1. Tan MK, Muhammad AA, Gorochov AV, Abdullah NA
    Zootaxa, 2024 Feb 20;5415(1):117-143.
    PMID: 38480212 DOI: 10.11646/zootaxa.5415.1.5
    Recent collection in Panti Forest Reserve in Johor, Peninsular Malaysia offered us the opportunity to better understand the species in southern Malay Peninsula. Two new species and one new genus of Meconematini are described here: Cercoteratura reflexa sp. n. and Platycercotura caricauda gen. et sp. n. We present new locality records for Peracca macritchiensis Tan & Ingrisch, 2014, Viriacca viridis Ingrisch, 1998; Lipotactes (Dialipotactes) maculatus Hebard, 1922; Asiophlugis rete Gorochov, 1998; and Arnobia pilipes tropica Gorochov, 1998, all of which were previously recorded in Pahang (Peninsular Malaysia) and Singapore, suggest that the distribution of these species is continuous between Pahang and Singapore. New locality record for Asiophlugis temasek temasek Gorochov & Tan, 2011, previously known only from Singapore, indicates that this subspecies is no longer endemic to Singapore.
    MeSH terms: Animals; Malaysia; Orthoptera*; Animal Distribution
  2. Hong Z, Anuar MSS, Grismer LL, Quah ESH
    Zootaxa, 2024 Feb 15;5410(4):519-532.
    PMID: 38480228 DOI: 10.11646/zootaxa.5410.4.3
    Species in the genus Pseudocalotes are generally rare and known only from a few specimens. Pseudocalotes drogon was described based on a single male from Frasers Hill, Pahang, Peninsular Malaysia. During a recent field survey at Frasers Hill, a female specimen was collected and identified as P. drogon based on morphological characters and a molecular phylogenetic analysis. The morphological description of P. drogon is expanded here, based on the male holotype and the newly collected female specimen.
    MeSH terms: Animals; Female; Lizards*; Malaysia; Male; Phylogeny; Snakes*
  3. Tan MK, Muhammad AA, Abdullah NA, Japir R, Chung AYC
    Zootaxa, 2024 Feb 13;5410(2):267-279.
    PMID: 38480244 DOI: 10.11646/zootaxa.5410.2.7
    The taxonomy of the crickets from the genus Odontogryllodes Chopard, 1969 is reviewed. Two species new to science are described here, one from Peninsular Malaysia (part of Malay Peninsula) and another from East Malaysia (part of Borneo): Odontogryllodes undatus Tan, Muhammad & Abdullah sp. nov. from Panti Forest Reserve and Odontogryllodes magnus Tan, Japir & Chung sp. nov. from Tenompok Forest Reserve, respectively. We also present an updated key to the species of Odontogryllodes. In total, nine species are currently known.
    MeSH terms: Animals; Gryllidae*; Animal Distribution
  4. Amarasinghe AAT, Campbell PD, Riyanto A, Hallermann J, Hamidy A, Andayani N, et al.
    Zootaxa, 2024 Mar 13;5424(2):189-202.
    PMID: 38480290 DOI: 10.11646/zootaxa.5424.2.3
    Bronchocela celebensis Gray, 1845 is one of the rarest species of the genus, known only from less than 20 museum specimens collected from northern Sulawesi. It is often confused with its similar congener, B. cristatella, which occurs widely throughout the Indonesian Archipelago and Peninsular Malaysia, except on the Sulawesi mainland. Here, we examine the morphology of B. celebensis based on 46 museum specimens including freshly collected individuals, and redescribe the species based on the holotype (by monotypy). We studied the characters of B. celebensis with morphometric comparison to its allopatric congener B. cristatella from the adjacent islands of southern Sulawesi in Indonesia. Based on the current distribution pattern and the apparent threats, we update the conservation status of B. celebensis using the IUCN Red List Criteria and propose that it be considered as a Vulnerable (VU) species endemic to Sulawesi.
    MeSH terms: Animals; Indonesia; Lizards*; Museums
  5. Tan MK, Grumo KC, Gono AIA, Bahoy DCM, Rivera RR, Nueza OM, et al.
    Zootaxa, 2024 Mar 12;5424(1):61-79.
    PMID: 38480300 DOI: 10.11646/zootaxa.5424.1.3
    A new species of Itara (Phormincter) is described from Mindanao in the Philippines: Itara (Phormincter) mindanao Tan, Grumo, Gono & Bahoy, sp. nov. This represents the first record of this subgenus in the Philippines, having previously known only from Borneo, Java, Malay Peninsula and Sumatra; as well as only the second species of Itara known from the Philippines and the first from Mindanao Island. The male calling song of the holotype was also recorded and is described here. Additionally, the male calling song of another congener, Itara (Singitara) singularis Gorochov, 1997 from Sabah, is described here. A new species of Itara (Bornitara) is also described from Sabah in Borneo: Itara (Bornitara) tenompok Tan, Japir & Chung, sp. nov.
    MeSH terms: Animals; Malaysia; Male; Orthoptera*; Philippines; Animal Distribution
  6. Tan MK, Japir R, Chung AYC
    Zootaxa, 2024 Mar 08;5419(4):584-600.
    PMID: 38480307 DOI: 10.11646/zootaxa.5419.4.6
    Based on recent field sampling in the highlands of western Sabah, a new species of Depressacca Ingrisch, 1998 (Conocephalinae: Agraeciini) is described from Tenompok, Depressacca macrolima sp. nov., that can be easily distinguish from two other known congeners by the presence of numerous large and falcate spines on the legs numerous large and falcate spines on its legs. Based on the new material, we also document here the live images and/or new locality records for other katydids of the tribes Agraeciini and Meconematini: Bispinolakis longicauda Ingrisch, 1998, Palaeoagraecia philippina (Karny, 1926) and Salomona borneensis Willemse, 1959; Alloteratura (Meconemopsis) longa Gorochov, 2008, Borneratura kinabaluana (Bey-Bienko, 1971) and Rhinoteratura pulchra Gorochov, 2008.
    MeSH terms: Animals; Malaysia; Orthoptera*; Animal Distribution
  7. Donworth P, Wesener T
    Zootaxa, 2024 Mar 08;5419(4):545-562.
    PMID: 38480309 DOI: 10.11646/zootaxa.5419.4.4
    Thailand hosts a very rich but underexplored giant pill-millipede (Sphaerotheriida) fauna, with 11 of its 13 species described in the last three years. Currently, all known Thai giant pill-millipedes belong to the genera Zephronia Gray, 1832 (nine species) and Sphaerobelum Verhoeff, 1924 (four species). Here we describe the first two species of the genus Prionobelum Verhoeff, 1924 (previously restricted to Vietnam and China), Prionobelum inthanonense n. sp. and P. naevium n. sp. from Thailand. The species occur at Thailands highest mountain (2500 m) Doi Inthanon and the lowland rainforests at Bang Lang National Park touching the border with Malaysia. Both species are described integratively, utilizing light microscopy, scanning electron microscopy as well as DNA barcoding. Both new species of Prionobelum differ from other Zephroniidae species, as well as from one another, by more than 20% p-distance in the COI barcoding gene suggesting that potential closer related species are still awaiting discovery.
    MeSH terms: Animals; Microscopy, Electron, Scanning; Thailand
  8. Kluge N, Sivaruban T, Srinivasan P, Barathy S, Isack R
    Zootaxa, 2024 Mar 04;5418(5):528-550.
    PMID: 38480342 DOI: 10.11646/zootaxa.5418.5.5
    The European species Nigrobaetis gracilis (Bogoescu & Tabacaru 1957) and more than 19 Asian and African species of Nigrobaetis Kazlauskas (in Novikova & Kluge) 1987 belong to the subgenus Margobaetis Kang & Yang 1994, which is characterized by peculiar asymmetric eggs and narrow paraglossa of larval labium. A new synonymy is established: Nigrobaetis (Margobaetis) minutus (Mller-Liebenau 1984) = N. paramakalyani Kubendran & Balasubramanian in Kubendran et al. 2015 = N. sumbensis Kaltenbach & Gattolliat 2023, synn.n.; winged stages (male and female imagines and subimagines) and eggs of this species are described for the first time. N. (M.) minutus is widely distributed on Oriental Region, being revealed in West Malaysia, Southern India, Sumba and Sulawesi islands in Indonesia. Winged stages (male and female imagines and subimagines) and eggs of Nigrobaetis (Margobaetis) klugei Sivaruban et al. 2022 are described for the first time.
    MeSH terms: Animals; Animal Structures; Female; Larva; Male; Animal Distribution; Ephemeroptera*
  9. Tasnim J, Hashim NM, Han HC
    Cell Biochem Funct, 2024 Mar;42(2):e3967.
    PMID: 38480622 DOI: 10.1002/cbf.3967
    A drug interaction is a condition in which two or more drugs are taken at the same time. Type 2 diabetes mellitus is a significant contributor to polypharmacy. Proton pump inhibitors (PPIs) are often prescribed in combination with metformin or DPP-4 inhibitors (sitagliptin, saxagliptin, linagliptin, and alogliptin) or a combined dose of metformin and DPP-4 inhibitor to treat gastritis in diabetic patients. This review article mainly focused on evaluating the potential drug-drug interactions (DDIs) between PPIs (i.e. esomeprazole, lansoprazole, omeprazole, pantoprazole, and rabeprazole) with metformin and PPIs with DPP-4 inhibitors. The findings demonstrated the existence of pharmacokinetic and pharmacodynamic DDIs between the aforementioned PPIs with metformin and DPP-4 inhibitors, which could impact the biological activities (i.e., hypoglycemia) of these drugs. Moreover, this review suggested that esomeprazole could be the best drug in the PPI group to be prescribed simultaneously with metformin and DPP-4 inhibitors, as most of the antidiabetic drugs of this study did not show any interaction with esomeprazole. The findings of this study also revealed that both antidiabetic drugs and PPIs could have positive interactions as PPIs have the potential to lessen the gastrointestinal side effects of metformin and DPP-4 inhibitors. To achieve the greatest therapeutic impact with the fewest side effects, careful dose control of these drugs is required. So, more extensive research on both human and animal subjects are needed to ascertain the veracity of this hypothesis.
    MeSH terms: Animals; Drug Interactions; Humans; Hypoglycemic Agents/pharmacology; Hypoglycemic Agents/therapeutic use; Proton Pump Inhibitors/pharmacokinetics; Esomeprazole/pharmacology
  10. Mohd Hatta FH, Samsudin EZ, Aimran N, Ismail Z, Sapian NAM
    Risk Manag Healthc Policy, 2024;17:455-471.
    PMID: 38481392 DOI: 10.2147/RMHP.S439914
    INTRODUCTION: Among available workplace violence (WPV) interventions, only data-driven, worksite-based, and risk-based approach WPV interventions had moderate evidence for effectiveness in decreasing the risk of WPV. The Questionnaires to Assess Workplace Violence Risk Factors (QAWRF) had been previously developed to determine the level of WPV risk factors in each healthcare setting based on the tripartite perspective of key stakeholders to enable effective WPV interventions. This study aimed to determine the construct validity and test-retest validity of QAWRF.

    METHODS: QAWRF, a three-component instrument consisting of QAWRF-Administrators, QAWRF-Workers, and QAWRF-Clients, had previously undergone content validation, face validation, and internal consistency reliability testing. 965 respondents were recruited to examine the construct validity of QAWRF, and a subset of these (n = 90) were retested again at an interval of three weeks to assess its test-retest reliability. Confirmatory factor analysis (CFA) was performed, and fitness indices, average variance extracted, correlation coefficient, composite reliability, and intraclass correlation coefficient were determined.

    RESULTS: QAWRF-Administrator, QAWRF-Worker, and QAWRF-Client had acceptable factor loadings (≥0.6), absolute fit (Root Mean Square Error of Approximation > 0.1), incremental fit (Confirmatory Fit Index and Tucker Lewis Index > 0.9), parsimonious fit (Chi-square/degree of freedom < 5), correlation coefficient between construct (≤0.85), discriminant validity index, and construct reliability (≥0.6). CFA supported a four-factor model for QAWRF-Administrator and QAWRF-Worker, and a two-factor model for QAWRF-Client.

    CONCLUSION: QAWRF holds good construct validity and test-retest reliability. By using QAWRF, healthcare managers can identify specific WPV risk factors that are perceived by stakeholders as prevalent at a particular workplace, and these findings can contribute towards data-driven, worksite-specific, and targeted WPV interventions in healthcare settings that are expected to be resource-efficient and more effective than general WPV interventions.

  11. Debnath S, Elgorban AM, Bahkali AH, Eswaramoorthy R, Verma M, Syed A, et al.
    Front Plant Sci, 2024;15:1255979.
    PMID: 38481405 DOI: 10.3389/fpls.2024.1255979
    BACKGROUND: Brassica oleracea var. botrytis is an annual or biennial herbaceous vegetable plant in the Brassicaceae family notable for its edible blossom head. A lot of effort has gone into finding defense-associated proteins in order to better understand how cauliflower and pathogens interact. Endophytes are organisms that live within the host plant and reproduce. Endophytes are bacteria and fungi that reside in plant tissues and can either help or harm the plant. Several species have aided molecular biologists and plant biotechnologists in various ways. Water is essential for a healthy cauliflower bloom. When the weather is hot, this plant dries up, and nitrogen scarcity can be detrimental to cauliflower growth.

    OBJECTIVE: The study sought to discern plant growth promoting (PGP) compounds that can amplify drought resilience and boost productivity in cauliflower.

    METHODS: Investigations were centered on endophytes, microorganisms existing within plant tissues. The dual role of beneficial and detrimental Agrobacterium was scrutinized, particularly emphasizing the ethylene precursor compound, 1-amino-cyclopropane-1-carboxylic acid (ACCA).

    RESULTS: ACCA possessed salient PGP traits, particularly demonstrating a pronounced enhancement of drought resistance in cauliflower plants. Specifically, during the pivotal marketable curd maturity phase, which necessitates defense against various threats, ACCA showcased a binding energy of -8.74 kcal/mol.

    CONCLUSION: ACCA holds a significant promise in agricultural productivity, with its potential to boost drought resistance and cauliflower yield. This could be particularly impactful for regions grappling with high temperatures and possible nitrogen shortages. Future research should explore ACCA's performance under diverse environmental settings and its applicability in other crops.

  12. Hu X, Xie Y, Zhao H, Sheng G, Lai KW, Zhang Y
    PeerJ Comput Sci, 2024;10:e1874.
    PMID: 38481705 DOI: 10.7717/peerj-cs.1874
    Epilepsy is a chronic, non-communicable disease caused by paroxysmal abnormal synchronized electrical activity of brain neurons, and is one of the most common neurological diseases worldwide. Electroencephalography (EEG) is currently a crucial tool for epilepsy diagnosis. With the development of artificial intelligence, multi-view learning-based EEG analysis has become an important method for automatic epilepsy recognition because EEG contains difficult types of features such as time-frequency features, frequency-domain features and time-domain features. However, current multi-view learning still faces some challenges, such as the difference between samples of the same class from different views is greater than the difference between samples of different classes from the same view. In view of this, in this study, we propose a shared hidden space-driven multi-view learning algorithm. The algorithm uses kernel density estimation to construct a shared hidden space and combines the shared hidden space with the original space to obtain an expanded space for multi-view learning. By constructing the expanded space and utilizing the information of both the shared hidden space and the original space for learning, the relevant information of samples within and across views can thereby be fully utilized. Experimental results on a dataset of epilepsy provided by the University of Bonn show that the proposed algorithm has promising performance, with an average classification accuracy value of 0.9787, which achieves at least 4% improvement compared to single-view methods.
  13. Nowalid WFWM, Hamid HA, Giwa SH
    Food Chem (Oxf), 2024 Jul 30;8:100196.
    PMID: 38482042 DOI: 10.1016/j.fochms.2024.100196
    Natural slice jam from the citrus peel is a product that enhances the economic value of the waste of citrus fruit peel. The goal of the study was to improve a recipe for a natural jam made entirely from citrus peel and shaped into a square to make it a more convenient and ready-to-eat product. A two-level factorial design was used to investigate the effects of four independent variables; X1: concentration lemon peel (Citrus limonum), X2: concentration orange peel (Citrus sinensis), X3: storage temperature, and X4: sugar. Sample processing was performed at range between 10 and 20 g of lemon and orange peel concentration and sugar (6-10 g) at a constant gelatine weight of 1.6 g. The samples were stored at 2 °C and 25 °C, and textural properties (hardness and adhesiveness) were evaluated. Results indicated that concentrations of lemon peel (10 g), orange peel (15 g), sugar (8 g), and storage temperature (13.5 °C) significantly influenced jam texture. Interaction effects, including AD, BC, and BD, were notable. Optimal conditions favoured higher orange peel concentration. Validation experiments confirmed a maximum 10 g lemon peel concentration with an error below 10%. Despite room temperature storage causing textural quality deterioration due to syneresis, this study contributes valuable insights for food industry applications of natural products, greener and more cost-effective. The factorial design approach effectively optimized citrus peel slice jam formulation, demonstrating the significance of applied sciences in addressing practical challenges.
  14. Lim BWD, Abdul Latiff AH
    Asia Pac Allergy, 2024 Mar;14(1):1-4.
    PMID: 38482459 DOI: 10.5415/apallergy.0000000000000136
    Inborn errors of immunity (IEI) (also known as primary immunodeficiencies) is an umbrella term for a growing group of over 450 different disorders that are characterized by defects in some of the components of the immune system. IEI are chronic diseases of genetic origin that render individuals suffering from them susceptible to infections. The mainstay of treatments for most patients with IEI, that is, predominantly antibody deficiencies is immunoglobulin replacement therapy (IRT), which is commonly delivered intravenously. Immunoglobulin (IG) therapy contains antibodies to compensate for the defective immune system's inability to produce them. Individuals with IEI need IRT regularly throughout their lives to help combat infections and prevent organ damage. Without IRT, they are in danger of suffering from morbidity, poor quality of life, and reduced life expectancy. In the last 20 years, the use of IG preparation has tripled and this is partly attributed to the growing awareness and improved diagnoses of IEI cases. IG preparations are also used for the treatment of other medical conditions including secondary immunodeficiencies and autoimmune diseases. As IG is derived from human plasma, there are concerns about the availability of supply, particularly to treat life-threatening conditions that cannot be improved with other medications. It is estimated that 75% to 80% of IEI patients do not have access to adequate IG therapy throughout the world. This concern of supply and other challenges faced by patients with IEI in Malaysia are described from the patients' perspective.
  15. Kondo Y, Ohtsuka S, Nawata M, Nishida Y, Komeda S, Iwasaki S, et al.
    Dis Aquat Organ, 2024 Mar 14;157:81-94.
    PMID: 38483243 DOI: 10.3354/dao03773
    Before 2019, adults of the sea louse Caligus undulatus were reported exclusively in plankton from ocean samples worldwide and were not known to parasitize fish hosts. In 2019, the first instance of this caligid parasitizing a fish host, Japanese sardinella Sardinella zunasi, was reported in the Seto Inland Sea, Japan. The presently reported study aimed to investigate the biology and ecology of adult C. undulatus in plankton communities in the Seto Inland Sea and surrounding waters from March 2020 to November 2021. The occurrence of sea lice in plankton communities was restricted to the period of August-January, mainly between October and December with maximum plankton abundance (10.5 ind. per 1000 m3) recorded on 30 November 2020. All post-naupliar stages of C. undulatus were found on the host fish, and they represented a typical life cycle pattern known for Caligus species. The sex ratios in both planktonic and parasitic adults were not significantly different. The frequency of occurrence of planktonic and parasitic adult females with egg strings was 68 and 46%, respectively. The number of eggs per string was significantly higher in parasitic adult females (mean ± SD: 16.9 ± 8.6) than in planktonic females (10.4 ± 10.8). These data suggest that adult females were detached from their hosts and continued to produce eggs without feeding. Seasonal migration of S. zunasi to brackish water for spawning may result in the detachment of mature caligids from the host and may be effective in protecting the offspring, which are less tolerant of less brackish water.
    MeSH terms: Animals; Female; Fishes; Japan; Plankton; Ecosystem; Copepoda*
  16. Yang CH, Li XY, Lv JJ, Hou MJ, Zhang RH, Guo H, et al.
    JMIR Public Health Surveill, 2024 Mar 14;10:e55327.
    PMID: 38483459 DOI: 10.2196/55327
    BACKGROUND: Asthma has become one of the most common chronic conditions worldwide, especially among children. Recent findings show that the prevalence of childhood asthma has increased by 12.6% over the past 30 years, with >262 million people currently affected globally. The reasons for the growing asthma epidemic remain complex and multifactorial.

    OBJECTIVE: This study aims to provide an up-to-date analysis of the changing global and regional asthma prevalence, mortality, disability, and risk factors among children aged <20 years by leveraging the latest data from the Global Burden of Disease Study 2019. Findings from this study can help inform priority areas for intervention to alleviate the rising burden of childhood asthma globally.

    METHODS: The study used data from the Global Burden of Disease Study 2019, concentrating on children aged 0 to 14 years with asthma. We conducted an in-depth analysis of asthma, including its age-standardized prevalence, incidence, mortality, and disability-adjusted life years (DALYs), across diverse demographics, such as region, age, sex, and sociodemographic index, spanning 1990 to 2019. We also projected the future burden of the disease.

    RESULTS: Overall, in the Western Pacific Region, the age-standardized prevalence rate of asthma among children increased slightly, from 3898.4 cases per 100,000 people in 1990 to 3924 per 100,000 in 2019. The age-standardized incidence rate of asthma also increased slightly, from 979.2 to 994.9 per 100,000. In contrast, the age-standardized death rate of asthma decreased from 0.9 to 0.4 per 100,000 and the age-standardized DALY rate decreased from 234.9 to 189.7 per 100,000. At the country level, Japan experienced a considerable decrease in the age-standardized prevalence rate of asthma among children, from 6669.1 per 100,000 in 1990 to 5071.5 per 100,000 in 2019. Regarding DALYs, Japan exhibited a notable reduction, from 300.6 to 207.6 per 100,000. Malaysia also experienced a DALY rate reduction, from 188.4 to 163.3 per 100,000 between 1990 and 2019. We project that the burden of disease in countries other than Japan and the Philippines will remain relatively stable up to 2045.

    CONCLUSIONS: The study indicates an increase in the prevalence and incidence of pediatric asthma, coupled with a decrease in mortality and DALYs in the Western Pacific Region between 1990 and 2019. These intricate phenomena appear to result from a combination of lifestyle shifts, environmental influences, and barriers to health care access. The findings highlight that nations such as Japan have achieved notable success in managing asthma. Overall, the study identified areas of improvement in view of persistent disease burden, underscoring the need for comprehensive collaborative efforts to mitigate the impact of pediatric asthma throughout the region.

    MeSH terms: Adolescent; Child; Child, Preschool; Health Services Accessibility; Humans; Infant; Japan; Cost of Illness; Epidemics*
  17. Mohd Raub AA, Bahru R, Mohamed MA, Latif R, Mohammad Haniff MAS, Simarani K, et al.
    Nanotechnology, 2024 Apr 02;35(24).
    PMID: 38484390 DOI: 10.1088/1361-6528/ad33e8
    Nanostructured metal oxide semiconductors have emerged as promising nanoscale photocatalysts due to their excellent photosensitivity, chemical stability, non-toxicity, and biocompatibility. Enhancing the photocatalytic activity of metal oxide is critical in improving their efficiency in radical ion production upon optical exposure for various applications. Therefore, this review paper provides an in-depth analysis of the photocatalytic activity of nanostructured metal oxides, including the photocatalytic mechanism, factors affecting the photocatalytic efficiency, and approaches taken to boost the photocatalytic performance through structure or material modifications. This paper also highlights an overview of the recent applications and discusses the recent advancement of ZnO-based nanocomposite as a promising photocatalytic material for environmental remediation, energy conversion, and biomedical applications.
  18. Ahmad A, Bromberg DJ, Shrestha R, Salleh NM, Bazazi AR, Kamarulzaman A, et al.
    Int J Drug Policy, 2024 Apr;126:104369.
    PMID: 38484531 DOI: 10.1016/j.drugpo.2024.104369
    BACKGROUND: Incarcerated people with HIV and opioid-dependence often experience poor post-release outcomes in the absence of methadone maintenance treatment (MMT). In a prospective trial, we assessed the impact of methadone dose achieved within prison on linkage to MMT after release.

    METHODS: From 2010 to 2014, men with HIV (N = 212) and opioid dependence before incarceration were enrolled in MMT within 6 months of release from Malaysia's largest prison and followed for 12-months post-release. As a prospective trial, allocation to MMT was at random and later by preference design (predictive nonetheless). MMT dosing was individually targeted to minimally achieve 80 mg/day. Time-to-event analyses were conducted to model linkage to MMT after release.

    FINDINGS: Of the 212 participants allocated to MMT, 98 (46 %) were prescribed higher dosages (≥80 mg/day) before release. Linkage to MMT after release occurred in 77 (36 %) participants and significantly higher for those prescribed higher dosages (46% vs 28 %; p = 0.011). Factors associated with higher MMT dosages were being married, on antiretroviral therapy, longer incarceration periods, having higher levels of depression, and methadone preference compared to randomization. After controlling for other variables, being prescribed higher methadone dosage (aHR: 2.53, 95 %CI: 1.42-4.49) was the only independent predictor of linkage to methadone after release.

    INTERPRETATION: Higher doses of methadone prescribed before release increased the likelihood of linkage to MMT after release. Methadone dosing should be introduced into international guidelines for treatment of opioid use disorder in prisons and further post-release benefits should be explored.

    FUNDING: National Institute of Drug Abuse (NIDA).

    MeSH terms: Adult; Analgesics, Opioid/administration & dosage; Dose-Response Relationship, Drug; Humans; Malaysia; Male; Middle Aged; Prisoners*; Prisons; Prospective Studies; Opiate Substitution Treatment*
  19. Lim JR, Chua LS, Mustaffa AA
    PMID: 38484676 DOI: 10.1016/j.jchromb.2024.124072
    The peroxyl radicals generated by the activity of lipoxygenases (LOX) are mediators to trigger inflammatory diseases. Therefore, it is important to investigate potent LOX inhibitor for modulating the occurrence and resolving inflammatory processes. Artemisa vulgaris, is a herbal plant that is known for flavonoids, potentially inhibiting lipid peroxidation and scavenging radicals. The objectives of the present study were to obtain flavonoids rich extract from A. vulgaris, and determine the inhibitory mode of the extract against LOX. The flavonoids rich extract was optimized in an ultrasound assisted extraction using ionic liquids as extraction solvent. The results found that the optimum conditions; ratio of solid-to-liquid (1:10) and 30 min of extraction time could produce the high yield (10.14 %) and flavonoid content (5.30 mg QE/g). The LOX activity was demonstrated to follow a mixed mode of inhibition in the presence of the flavonoid rich extract as an inhibitor. The Michaelis-Menten constant (Km) was increased from 0.283 µM to 0.435 µM, whereas the maximum velocity was reduced from 0.22 µM/min to 0.058 µM/min in the inhibition. The flavonoids rich extract is likely to be a natural potent non-competitive inhibitor which may bind to free LOX or substrate-bound LOX.
    MeSH terms: Antioxidants/pharmacology; Plant Extracts/pharmacology; Artemisia*; Lipoxygenases
  20. GBD 2021 Demographics Collaborators
    Lancet, 2024 May 18;403(10440):1989-2056.
    PMID: 38484753 DOI: 10.1016/S0140-6736(24)00476-8
    BACKGROUND: Estimates of demographic metrics are crucial to assess levels and trends of population health outcomes. The profound impact of the COVID-19 pandemic on populations worldwide has underscored the need for timely estimates to understand this unprecedented event within the context of long-term population health trends. The Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2021 provides new demographic estimates for 204 countries and territories and 811 additional subnational locations from 1950 to 2021, with a particular emphasis on changes in mortality and life expectancy that occurred during the 2020-21 COVID-19 pandemic period.

    METHODS: 22 223 data sources from vital registration, sample registration, surveys, censuses, and other sources were used to estimate mortality, with a subset of these sources used exclusively to estimate excess mortality due to the COVID-19 pandemic. 2026 data sources were used for population estimation. Additional sources were used to estimate migration; the effects of the HIV epidemic; and demographic discontinuities due to conflicts, famines, natural disasters, and pandemics, which are used as inputs for estimating mortality and population. Spatiotemporal Gaussian process regression (ST-GPR) was used to generate under-5 mortality rates, which synthesised 30 763 location-years of vital registration and sample registration data, 1365 surveys and censuses, and 80 other sources. ST-GPR was also used to estimate adult mortality (between ages 15 and 59 years) based on information from 31 642 location-years of vital registration and sample registration data, 355 surveys and censuses, and 24 other sources. Estimates of child and adult mortality rates were then used to generate life tables with a relational model life table system. For countries with large HIV epidemics, life tables were adjusted using independent estimates of HIV-specific mortality generated via an epidemiological analysis of HIV prevalence surveys, antenatal clinic serosurveillance, and other data sources. Excess mortality due to the COVID-19 pandemic in 2020 and 2021 was determined by subtracting observed all-cause mortality (adjusted for late registration and mortality anomalies) from the mortality expected in the absence of the pandemic. Expected mortality was calculated based on historical trends using an ensemble of models. In location-years where all-cause mortality data were unavailable, we estimated excess mortality rates using a regression model with covariates pertaining to the pandemic. Population size was computed using a Bayesian hierarchical cohort component model. Life expectancy was calculated using age-specific mortality rates and standard demographic methods. Uncertainty intervals (UIs) were calculated for every metric using the 25th and 975th ordered values from a 1000-draw posterior distribution.

    FINDINGS: Global all-cause mortality followed two distinct patterns over the study period: age-standardised mortality rates declined between 1950 and 2019 (a 62·8% [95% UI 60·5-65·1] decline), and increased during the COVID-19 pandemic period (2020-21; 5·1% [0·9-9·6] increase). In contrast with the overall reverse in mortality trends during the pandemic period, child mortality continued to decline, with 4·66 million (3·98-5·50) global deaths in children younger than 5 years in 2021 compared with 5·21 million (4·50-6·01) in 2019. An estimated 131 million (126-137) people died globally from all causes in 2020 and 2021 combined, of which 15·9 million (14·7-17·2) were due to the COVID-19 pandemic (measured by excess mortality, which includes deaths directly due to SARS-CoV-2 infection and those indirectly due to other social, economic, or behavioural changes associated with the pandemic). Excess mortality rates exceeded 150 deaths per 100 000 population during at least one year of the pandemic in 80 countries and territories, whereas 20 nations had a negative excess mortality rate in 2020 or 2021, indicating that all-cause mortality in these countries was lower during the pandemic than expected based on historical trends. Between 1950 and 2021, global life expectancy at birth increased by 22·7 years (20·8-24·8), from 49·0 years (46·7-51·3) to 71·7 years (70·9-72·5). Global life expectancy at birth declined by 1·6 years (1·0-2·2) between 2019 and 2021, reversing historical trends. An increase in life expectancy was only observed in 32 (15·7%) of 204 countries and territories between 2019 and 2021. The global population reached 7·89 billion (7·67-8·13) people in 2021, by which time 56 of 204 countries and territories had peaked and subsequently populations have declined. The largest proportion of population growth between 2020 and 2021 was in sub-Saharan Africa (39·5% [28·4-52·7]) and south Asia (26·3% [9·0-44·7]). From 2000 to 2021, the ratio of the population aged 65 years and older to the population aged younger than 15 years increased in 188 (92·2%) of 204 nations.

    INTERPRETATION: Global adult mortality rates markedly increased during the COVID-19 pandemic in 2020 and 2021, reversing past decreasing trends, while child mortality rates continued to decline, albeit more slowly than in earlier years. Although COVID-19 had a substantial impact on many demographic indicators during the first 2 years of the pandemic, overall global health progress over the 72 years evaluated has been profound, with considerable improvements in mortality and life expectancy. Additionally, we observed a deceleration of global population growth since 2017, despite steady or increasing growth in lower-income countries, combined with a continued global shift of population age structures towards older ages. These demographic changes will likely present future challenges to health systems, economies, and societies. The comprehensive demographic estimates reported here will enable researchers, policy makers, health practitioners, and other key stakeholders to better understand and address the profound changes that have occurred in the global health landscape following the first 2 years of the COVID-19 pandemic, and longer-term trends beyond the pandemic.

    FUNDING: Bill & Melinda Gates Foundation.

    MeSH terms: Adolescent; Adult; Aged; Aged, 80 and over; Child; Child, Preschool; Demography; Female; Humans; Infant; Infant, Newborn; Male; Middle Aged; Mortality/trends; Age Distribution; Young Adult; Pandemics; Global Burden of Disease*
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