Product spectrum analysis and microbial insights of medium-chain fatty acids production from waste biomass during liquor fermentation process: Effects of substrate concentrations and fermentation modes

Zhao J 1 , Ma H 2 , Wu W 1 , Ali Bacar M 1 , Wang Q 1 , Gao M 1 Show all authors , Wu C 1 , Xia C 3 , Qian D 4 , Chong WWF 5 , Lam SS 6

Affiliations 

  • 1 Department of Environmental Science and Engineering, University of Science and Technology, Beijing, Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, Beijing 100083, China
  • 2 Department of Environmental Science and Engineering, University of Science and Technology, Beijing, Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, Beijing 100083, China; Key Laboratory of Pollutant Chemistry and Environmental Treatment, School of Chemistry and Environmental Science, Yili Normal University, Yining 835000, China. Electronic address: mahongzhi@ustb.edu.cn
  • 3 Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, College of Materials Science and Engineering, Nanjing Forestry University, Nanjing, Jiangsu 210037, China
  • 4 Department of Environmental Science and Engineering, University of Science and Technology, Beijing, Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, Beijing 100083, China; Key Laboratory of Pollutant Chemistry and Environmental Treatment, School of Chemistry and Environmental Science, Yili Normal University, Yining 835000, China
  • 5 Automotive Development Centre (ADC), Institute for Vehicle Systems and Engineering (IVeSE), Universiti Teknologi Malaysia (UTM), Johor Bahru 81310, Johor, Malaysia
  • 6 Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia; Automotive Development Centre (ADC), Institute for Vehicle Systems and Engineering (IVeSE), Universiti Teknologi Malaysia (UTM), Johor Bahru 81310, Johor, Malaysia
Bioresour Technol, 2023 Jan;368:128375.
PMID: 36414142 DOI: 10.1016/j.biortech.2022.128375

Abstract

Substrate toxicity would limit the upgrading of waste biomass to medium-chain fatty acids (MCFAs). In this work, two fermentation modes of electro-fermentation (EF) and traditional fermentation (TF) with different concentration of liquor fermentation waste (20%, 40%, 60%) were used for MCFAs production as well as mechanism investigation. The highest caproate (4.04 g/L) and butyrate (13.96 g/L) concentrations were obtained by EF at 40% substrate concentration. TF experiments showed that the substrate concentration above 40% severely inhibited ethanol oxidation and products formation. Compared with TF mode, the total substrates consumption and product yields under EF mode were significantly increased by 2.6%-43.5% and 54.0%-83.0%, respectively. Microbial analysis indicated that EF effectively alleviated substrate toxicity and enriched chain elongation bacteria, particularly Clostridium_sensu_stricto 12, thereby promoting ethanol oxidation and products formation. Caproiciproducens tolerated high-concentration substrates to ensure normal lactate metabolism. This study provides a new way to produce MCFAs from high concentration wastewater.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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