Reactive Green 19 dye-ligand immobilized on the aminated nanofiber membranes for efficient adsorption of lysozyme: Process development and optimization in batch and flow systems

Hsu JL 1 , Wang SS 2 , Ooi CW 3 , Thew XEC 4 , Lai YR 2 , Chiu CY 1 Show all authors , Hsu M 5 , Chen KH 6 , Chang YK 7

Affiliations 

  • 1 Department of Chemical Engineering, Graduate School of Biochemical Engineering, Ming Chi University of Technology, Taishan Dist., New Taipei City 243303, Taiwan
  • 2 Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan
  • 3 Chemical Engineering Discipline, School of Engineering, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor, Malaysia; Advanced Engineering Platform, School of Engineering, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor, Malaysia
  • 4 Chemical Engineering Discipline, School of Engineering, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor, Malaysia
  • 5 Chemist Scientific Corp., Taishan Dist., New Taipei City 243303, Taiwan
  • 6 Department of Chemical Engineering, Graduate School of Biochemical Engineering, Ming Chi University of Technology, Taishan Dist., New Taipei City 243303, Taiwan. Electronic address: khchen@mail.mcut.edu.tw
  • 7 Department of Chemical Engineering, Graduate School of Biochemical Engineering, Ming Chi University of Technology, Taishan Dist., New Taipei City 243303, Taiwan; Department of Chemical Engineering and Materials Science, Yuan Ze University, Zhongli Dist., Taoyuan City 320315, Taiwan. Electronic address: ykchang@mail.mcut.edu.tw
Food Chem, 2023 Apr 16;406:135028.
PMID: 36446280 DOI: 10.1016/j.foodchem.2022.135028

Abstract

The performance of lysozyme adsorption by the aminated nanofiber membrane immobilized with Reactive Green 19 (RG19) dyes was evaluated in batch and flow systems. The physicochemical properties of the dye-immobilized nanofiber membrane were characterized. The parameters of batch-mode adsorption of lysozyme (e.g., pH, initial dye concentration, and lysozyme concentration) were optimized using the Taguchi method. In a flow process, the factors influencing the dynamic binding performance for lysozyme adsorption in the chicken egg white (CEW) solution include immobilized dye concentration, adsorption pH value, feed flow rate, and feed CEW concentration. The impact of these operating conditions on the lysozyme purification process was investigated. Under optimal conditions, the recovery yield and purification factor of lysozyme achieved from the one-step adsorption process were 98.52% and 143 folds, respectively. The dye-affinity nanofiber membrane also did not exhibit any significant loss in its binding capacity and purification performance after five consecutive uses.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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