Effective purification of lysozyme from chicken egg white by tris(hydroxymethyl)aminomethane affinity nanofiber membrane

Liu BL 1 , Ooi CW 2 , Ng IS 3 , Show PL 4 , Lin KJ 5 , Chang YK 6

Affiliations 

  • 1 Department of Applied Chemistry, Chaoyang University of Technology, Taichung 41349, Taiwan
  • 2 Chemical Engineering Discipline, School of Engineering, School of Engineering, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor, Malaysia
  • 3 Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan
  • 4 Department of Chemical and Environmental Engineering, The University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor, Malaysia
  • 5 Department of Chemical Engineering/Graduate School of Biochemical Engineering, Ming Chi University of Technology, New Taipei City 24301, Taiwan
  • 6 Department of Chemical Engineering/Graduate School of Biochemical Engineering, Ming Chi University of Technology, New Taipei City 24301, Taiwan. Electronic address: ykchang@mail.mcut.edu.tw
Food Chem, 2020 Oct 15;327:127038.
PMID: 32447136 DOI: 10.1016/j.foodchem.2020.127038

Abstract

Polyacrylonitrile nanofiber membrane functionalized with tris(hydroxymethyl)aminomethane (P-Tris) was used in affinity membrane chromatography for lysozyme adsorption. The effects of pH and protein concentration on lysozyme adsorption were investigated. Based on Langmuir model, the adsorption capacity of P-Tris nanofiber membrane was estimated to be 345.83 mg/g. For the operation of dynamic membrane chromatography with three-layer P-Tris nanofiber membranes, the optimal operating conditions were at pH 9, 1.0 mL/min of feed flow rate, and 2 mg/mL of feed concentration. Chicken egg white (CEW) was applied as the crude feedstock of lysozyme in the optimized dynamic membrane chromatography. The percent recovery and purification factor of lysozyme obtained from the chromatography were 93.28% and 103.98 folds, respectively. Our findings demonstrated the effectiveness of P-Tris affinity nanofiber membrane for the recovery of lysozyme from complex CEW solution.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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