Affiliations 

  • 1 Department of Chemical Engineering, Faculty of Engineering and Science, Universiti Tunku Abdul Rahman, Sungai Long Campus, Kajang 43000, Selangor, Malaysia
  • 2 Department of Applied Chemistry, Chaoyang University of Technology, Taichung 413310, Taiwan
  • 3 Graduate School of Biochemical Engineering, Ming Chi University of Technology, New Taipei City 243303, Taiwan
  • 4 Graduate School of Biochemical Engineering, Ming Chi University of Technology, New Taipei City 243303, Taiwan. Electronic address: ykchang@mail.mcut.edu.tw
Food Chem, 2021 Feb 15;338:128144.
PMID: 33092004 DOI: 10.1016/j.foodchem.2020.128144

Abstract

A weak ion-exchange membrane (P-COOH) was synthesized by alkaline hydrolysis of a polyacrylonitrile nanofiber membrane prepared by electrospinning process. The P-COOH membrane was characterized for its physical properties and its application for purification of lysozyme from chicken egg white was investigated. The lysozyme adsorption efficiency of the P-COOH membrane operating in a stirred cell contactor (Millipore, Model 8010) was evaluated. The effects of key parameters such as the feed concentration, the rotating speed, the flow rate of feed and the operating pressure were studied. The results showed successful purification of lysozyme with a high recovery yield of 98% and a purification factor of 63 in a single step. The purification strategy was scaled-up to the higher feedstock loading volume of 32.7 and 70 mL using stirred cell contactors of Model 8050 and 8200, respectively. The scale-up processes achieved similar purification results, proving linear scalability of the purification technique adopted.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.