Affiliations 

  • 1 Institute of Advanced Technology, University Putra Malaysia, Serdang, Selangor, Malaysia
  • 2 Institute of Advanced Technology, University Putra Malaysia, Serdang, Selangor, Malaysia; Department of Chemistry, Faculty of Science, University Putra Malaysia, Serdang, Selangor, Malaysia
  • 3 Department of Chemistry, Faculty of Science, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran
PLoS One, 2014;9(5):e96686.
PMID: 24809346 DOI: 10.1371/journal.pone.0096686

Abstract

In this paper, the electrochemical behavior of myricetin on a gold nanoparticle/ethylenediamine/multi-walled carbon-nanotube modified glassy carbon electrode (AuNPs/en/MWCNTs/GCE) has been investigated. Myricetin effectively accumulated on the AuNPs/en/MWCNTs/GCE and caused a pair of irreversible redox peaks at around 0.408 V and 0.191 V (vs. Ag/AgCl) in 0.1 mol L-1 phosphate buffer solution (pH 3.5) for oxidation and reduction reactions respectively. The heights of the redox peaks were significantly higher on AuNPs/en/MWNTs/GCE compare with MWCNTs/GC and there was no peak on bare GC. The electron-transfer reaction for myricetin on the surface of electrochemical sensor was controlled by adsorption. Some parameters including pH, accumulation potential, accumulation time and scan rate have been optimized. Under the optimum conditions, anodic peak current was proportional to myricetin concentration in the dynamic range of 5.0×10-8 to 4.0×10-5 mol L-1 with the detection limit of 1.2×10-8 mol L-1. The proposed method was successfully used for the determination of myricetin content in tea and fruit juices.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.